http://lod.bco-dmo.org/id/dataset/716979
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-10-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010.
2017-10-12
publication
2017-10-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-19
publication
https://doi.org/10.1575/1912/bco-dmo.716979.1
Dr Brian Binder
University of Georgia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Binder, B. (2017) Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-10-12 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.716979.1 [access date]
Prochlorococcus and Synechococcus cell counts in dilution experiment treatments. Dataset Description: <p>Prochlorococcus and Synechococcus cell counts in dilution experiment treatments.</p> Methods and Sampling: <div>
<div>Dilution Experiments: For an overview of the purpose and interpretation of dilution experiments see Landry (1993) and Worden &amp; Binder (2003). Seawater samples were taken with Go-Flo bottles suspended on non-metallic cable. A portion of this water was gravity-filtered through 0.2 um pore-size capsule filters (Whatman Polycap 36 TC), and appropriate volumes of filtered and unfiltered seawater were added to 500 ml polycarbonate bottles to achieve the indicated dilutions. Time-0 (T0) samples were removed from each bottle and preserved for later flow cytometric analysis as described below. Incubation bottles were then distributed to nylon mesh bags and resuspended in the water column at the depths indicated. After 24 hours, the bottles were recovered and sampled for time-final (TF) counts. All sampling gear, filters, and incubation bottles were acid-washed per Fitzwater et al. (1982). The dates, times, and locations of the experiments were as follows:</div>
<div>&nbsp;</div>
<div>Cruise&nbsp; &nbsp; &nbsp; Exper&nbsp; &nbsp; Date (UTC)&nbsp; &nbsp; T0 (UTC)&nbsp; &nbsp; &nbsp;E Long&nbsp; &nbsp; &nbsp;N Lat</div>
<div>CH0409&nbsp; &nbsp; X1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;26-May-09&nbsp; &nbsp; &nbsp;17:58:30&nbsp; &nbsp; &nbsp;-71.998&nbsp; &nbsp; 30.171</div>
<div>CH0409&nbsp; &nbsp; X2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;29-May-09&nbsp; &nbsp; &nbsp;13:28:09&nbsp; &nbsp; &nbsp;-72.002&nbsp; &nbsp; 30.173</div>
<div>CH0510&nbsp; &nbsp; X2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;27-May-10&nbsp; &nbsp; &nbsp;13:33:30&nbsp; &nbsp; &nbsp;-72.683&nbsp; &nbsp; 30.699</div>
<div>&nbsp;</div>
<div>Flow Cytometric Cell Counts: Samples were fixed with freshly titrated paraformaldehyde (pH 7.4–8.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a ‑80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order and defrosted in a 30 deg C water bath (just long enough to melt, ~5 min). Prior to analysis, polystyrene fluorescent beads (Flow Check® 0.494 um “BB”; Polysicences Inc., Washington, PA, USA), were added to each sample, and used to normalize cellular light scatter and fluorescence. Samples were typically run at an infusion rate of 20 uL min-1 for 1 to 20 min, depending on cell abundance within the sample. A minimum of 1,000 Prochlorococcus cells were analyzed, except for samples in which low cell concentrations made this impractical. Final cell concentrations tabulated here were calculated from 1-7 replicate counts (Count.n).</div>
</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0751672 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0751672
completed
Dr Brian Binder
University of Georgia
706-742-2399
Department of Marine Sciences Marine Science Building University of Georgia
Athens
GA
30602-3636
USA
bbinder@uga.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Exper
Bottle
Depth_Sample
Depth_Incubate
Dilution
Time
Pro
Syn
Count
Coulter-EPICS 753 flow cytometer
Go-flo bottle
theme
None, User defined
Cruise identfier
experiment id
bottle
depth_w
depth incubated
dilution
No BCO-DMO term
cell_concentration
count
featureType
BCO-DMO Standard Parameters
Flow Cytometer
GO-FLO Bottle
instrument
BCO-DMO Standard Instruments
CH0409
CH0510
service
Deployment Activity
Beaufort, North Carolina
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
https://osprey.bco-dmo.org/project/709339
Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
<p>The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general.<br />
Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum.<br />
The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.</p>
Picophytoplankton_Regulation
largerWorkCitation
project
eng; USA
oceans
Beaufort, North Carolina
-72.8769
-71.8634
30.1464
30.9082
2009-01-01
2010-12-31
Western Sargasso Sea (vicinity of 30 N 72 W)
0
BCO-DMO catalogue of parameters from Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/716989.rdf
Name: Cruise
Units: unitless
Description: <p>R/V Cape Hatteras Cruise Designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/716990.rdf
Name: Exper
Units: unitless
Description: <p>Experiment Designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/716991.rdf
Name: Bottle
Units: unitless
Description: <p>Unique incubation bottle identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/716992.rdf
Name: Depth_Sample
Units: meters
Description: <p>Depth of source water</p>
http://lod.bco-dmo.org/id/dataset-parameter/716993.rdf
Name: Depth_Incubate
Units: meters
Description: <p>Depth of incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/716994.rdf
Name: Dilution
Units: number
Description: <p>Fraction of unfiltered seawater in bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/716995.rdf
Name: Time
Units: unitless
Description: <p>Sampling time; timepoints</p>
http://lod.bco-dmo.org/id/dataset-parameter/716996.rdf
Name: Pro
Units: cells per milliliter
Description: <p>Prochlorococcus Cell Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/716997.rdf
Name: Syn
Units: cells per milliliter
Description: <p>Synechococcus Cell Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/716998.rdf
Name: Count
Units: number
Description: <p>Number of counts underlying cell concentrations</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11043
https://darchive.mblwhoilibrary.org/bitstream/1912/23860/1/dataset-716979_cell-counts-dilution-experiment__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.716979.1
download
onLine
dataset
<div>
<div>Dilution Experiments: For an overview of the purpose and interpretation of dilution experiments see Landry (1993) and Worden &amp; Binder (2003). Seawater samples were taken with Go-Flo bottles suspended on non-metallic cable. A portion of this water was gravity-filtered through 0.2 um pore-size capsule filters (Whatman Polycap 36 TC), and appropriate volumes of filtered and unfiltered seawater were added to 500 ml polycarbonate bottles to achieve the indicated dilutions. Time-0 (T0) samples were removed from each bottle and preserved for later flow cytometric analysis as described below. Incubation bottles were then distributed to nylon mesh bags and resuspended in the water column at the depths indicated. After 24 hours, the bottles were recovered and sampled for time-final (TF) counts. All sampling gear, filters, and incubation bottles were acid-washed per Fitzwater et al. (1982). The dates, times, and locations of the experiments were as follows:</div>
<div>&nbsp;</div>
<div>Cruise&nbsp; &nbsp; &nbsp; Exper&nbsp; &nbsp; Date (UTC)&nbsp; &nbsp; T0 (UTC)&nbsp; &nbsp; &nbsp;E Long&nbsp; &nbsp; &nbsp;N Lat</div>
<div>CH0409&nbsp; &nbsp; X1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;26-May-09&nbsp; &nbsp; &nbsp;17:58:30&nbsp; &nbsp; &nbsp;-71.998&nbsp; &nbsp; 30.171</div>
<div>CH0409&nbsp; &nbsp; X2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;29-May-09&nbsp; &nbsp; &nbsp;13:28:09&nbsp; &nbsp; &nbsp;-72.002&nbsp; &nbsp; 30.173</div>
<div>CH0510&nbsp; &nbsp; X2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;27-May-10&nbsp; &nbsp; &nbsp;13:33:30&nbsp; &nbsp; &nbsp;-72.683&nbsp; &nbsp; 30.699</div>
<div>&nbsp;</div>
<div>Flow Cytometric Cell Counts: Samples were fixed with freshly titrated paraformaldehyde (pH 7.4–8.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a ‑80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order and defrosted in a 30 deg C water bath (just long enough to melt, ~5 min). Prior to analysis, polystyrene fluorescent beads (Flow Check® 0.494 um “BB”; Polysicences Inc., Washington, PA, USA), were added to each sample, and used to normalize cellular light scatter and fluorescence. Samples were typically run at an infusion rate of 20 uL min-1 for 1 to 20 min, depending on cell abundance within the sample. A minimum of 1,000 Prochlorococcus cells were analyzed, except for samples in which low cell concentrations made this impractical. Final cell concentrations tabulated here were calculated from 1-7 replicate counts (Count.n).</div>
</div>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- removed rows that had all blank cells<br />
- replaced all decimal points in column headers with underscores<br />
- removed units from column names</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Coulter-EPICS 753 flow cytometer
Coulter-EPICS 753 flow cytometer
PI Supplied Instrument Name: Coulter-EPICS 753 flow cytometer PI Supplied Instrument Description:Used to analyze preserved samples Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Go-flo bottle
Go-flo bottle
PI Supplied Instrument Name: Go-flo bottle PI Supplied Instrument Description:Used to take seawater samples Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
Cruise: CH0409
CH0409
R/V Cape Hatteras
Community Standard Description
International Council for the Exploration of the Sea
R/V Cape Hatteras
vessel
CH0409
https://ezid.cdlib.org/id/doi:10.7284/902620
Report describing CH0409
Cruise: CH0510
CH0510
R/V Cape Hatteras
Community Standard Description
International Council for the Exploration of the Sea
R/V Cape Hatteras
vessel
CH0510
https://ezid.cdlib.org/id/doi:10.7284/901958
Report describing CH0510
R/V Cape Hatteras
Community Standard Description
International Council for the Exploration of the Sea
R/V Cape Hatteras
vessel