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&lt;p&gt;N2 fixation is a critical and intensely studied metabolism in the marine photic zone. Much less is known about N2 fixation in deep-sea sediments, but it could be an important factor in both benthic productivity and ocean-scale elemental cycling. Several observations have suggested or directly detected N2 fixation at localized areas of enhanced productivity on the seafloor (e.g., methane seeps and hydrothermal vents), raising the possibility that deep-sea N2 fixation is widespread. However, few measurements of N2 fixation have been made outside of these anomalous areas, and thus little is known about N2 fixation in the vast majority of the deep ocean floor. Preliminary data suggest N2 fixation does occur in typical deep marine sediment, and is mediated by a diverse set of yet unidentified microorganisms. This project will combine techniques from molecular biology and geochemistry to systematically investigate N2 fixation in representative deep-sea sediments collected along a depth profile (500 to 4500 m water depth) offshore California. The project will determine the (1) rates and distribution of N2 fixation (2) abundance, diversity, and distribution of genes and transcripts associated with N2 fixation (nif) (3) phylogenetic identity of the biological mediators (diazotrophs) and (4) physiochemical controls on diazotrophic community structure and activity. For context, the activity of the non-diazotrophic bacterial community will also be characterized. The results may lead to upward revisions of the estimates of new nitrogen production in the seafloor, and therefore change our understanding of the current balance of the marine nitrogen cycle. Together, this hypothesis-driven characterization of N2 fixation in deep-sea sediments will shed light on an expansive, climatically important, and traditionally understudied habitat, and facilitate more accurate extrapolation of the rates and distribution of N2 fixation on the whole seafloor as well as the metabolic response of the seafloor community to environmental change.&lt;/p&gt;</gco:CharacterString>
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&amp;amp;nbsp;*&amp;amp;nbsp;&amp;lt;a href=&amp;quot;http://dmoserv3.bco-dmo.org/data_docs/Deep_Sediment_N_Fix/OC1703A_CTD.zip&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;OC1703A_CTD.zip&amp;lt;/a&amp;gt;&amp;amp;nbsp;(11.2 MB)&amp;lt;br /&amp;gt;
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&amp;lt;br /&amp;gt;
&amp;lt;strong&amp;gt;BCO-DMO Data Manager Processing Notes:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
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