http://lod.bco-dmo.org/id/dataset/719172
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-11-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Elemental ratio data from LA ICP-MS analysis of oyster larvae exposed to various temperature, salinity, and elemental concentration treatments conducted in December of 2013 (EstuarineMetaDyn project)
2018-01-25
publication
2018-01-25
revision
BCO-DMO Linked Data URI
2018-01-25
creation
http://lod.bco-dmo.org/id/dataset/719172
F. Joel Fodrie
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fodrie, F. J. (2018) Elemental ratio data from LA ICP-MS analysis of oyster larvae exposed to various temperature, salinity, and elemental concentration treatments conducted in December of 2013 (EstuarineMetaDyn project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2018-01-25 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/719172 [access date]
Oyster larvae elemental ratio experiments Dataset Description: <p>This dataset contains elemental ratio data from LA ICP-MS analysis of oyster larvae exposed to various temperature, salinity, and elemental concentration treatments.</p> Methods and Sampling: <p>Sampling and Analytical Methodology:&nbsp;</p>
<p>To investigate environmental effects on larval (prodissoconch) shell signatures, we manipulated temperature, salinity, and elemental concentration of the water surrounding developing oyster larvae. Individual tanks were set up with the following treatments: low (21°C) or high (26.5°C) temperature; low (12.5 ppt) or high (20 ppt) salinity; and ambient (no addition), mid spike (+16 ppb Mn/0.16 ppb Pb addition), or elevated spike (+32 ppb Mn/0.32 ppb Pb) in concentrations of aqueous Mn and Pb. Temperature and salinity treatments were selected based on representative high and low observations in Pamlico Sound at the time of the experiment (mid-September). Trace metal spikes were calculated to increase the ambient levels of Mn and Pb in seawater, as measured by Becker et al. (2005), by 400% and 800% for mid and elevated spike levels, respectively.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Three-day old <em>C. virginica</em> larvae were obtained from the University of Maryland’s Horn Point Laboratory in Cambridge, Maryland, USA. Upon arrival at the Institute of Marine sciences (IMS) in Morehead City, NC, larvae were divided equally into 2, 1.2 L aerated holding tanks filled with a 12.5 ppt seawater mix (ultrapure H<sub>2</sub>O added to filtered seawater from Bogue Sound, NC). Over the next 4 days, larvae were acclimatized, with one tank receiving a salinity increase of approximately 2 ppt per day, resulting in a final salinity of 20 ppt, while the other tank remained at 12.5 ppt.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; After the acclimatization process was complete, larvae from both holding tanks, now 7 days old, were divided equally into 72 “larval homes”, with approximately 1.6 x 10<sup>4</sup> larvae per home (21.2 larvae cm<sup>-3</sup>). Larval homes were constructed from hollow PVC tubing capped on each side with nitex cloth, with a 30 μm mesh opening, to allow for the flow of water and food into the home, but prevent larvae from escaping. Homes were then placed into 24 aerated aquarium tanks (35 L), with 3 homes per tank. All tubing, PVC, air stones, and nitex were soaked in a HNO<sub>3</sub> solution and then rinsed thoroughly with ultrapure H<sub>2</sub>O prior to its use in the experiment.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Temperatures were maintained at either high or low level by 150 W Aquatop aquarium heaters and salinity levels were established by mixing filtered seawater with ultrapure H<sub>2</sub>O until desired salinity was reached. Mn and Pb concentrations were spiked by the addition of 545 μl of Mn + 5.45 μl of Pb and 1090 μl of Mn + 10.90 μl of Pb, from1000 ppt Fisher Scientific reference standard solutions, for mid and elevated spike treatments, respectively. Water changes were conducted every other day by removing one-third (~12 L) of water from the tank and replacing it with a freshly made mix. Tanks with mid or elevated spike treatments were then re-spiked to maintain consistent trace element concentrations. Immediately following water changes, larvae were fed by depositing dilute Instant Algae Shellfish Diet 1800 (Reed Mariculture; Campbell, California, USA) into larval homes via syringe. Each treatment group was crossed, to produce a full factorial design with 12 total treatment combinations. The experiment ran for 7 days, until the larvae were 14 days old.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Dissolved oxygen, temperature and salinity were monitored daily with a HACH HQ40d dual input, multi-parameter portable water quality meter. Dissolved oxygen, pH, salinity and temperature measures remained consistent among the treatments throughout our laboratory experiments. Mean dissolved oxygen and pH were 8.68 ± 0.025 mg L<sup>-1</sup> and 7.72 ± 0.032, respectively. Mean salinity for high and low salinity treatments were 20.7 ±0.091 ppt and 12.8± 0.120 ppt, respectively. Mean temperature for high temperature treatments was 25.7 ± 0.157 °C and 21.3 ±0.104 °C for low temperature treatments.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; At the conclusion of these mesocosm incubations, larvae from each home were filtered using nitex cloth (30 μm) and then resuspended in 15 mL of water from their respective tank. A 0.5-1 mL subsample of each larval resuspension was removed and the number of whole larvae were counted. The remaining larval solution was then frozen at -23°C until sample preparation for geochemical analysis.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Frozen larvae were thawed and approximately 1000 larvae were obtained representing each replicate home. The larvae were then rinsed with ultrapure H<sub>2</sub>O and mounted as a concentrated mass on a labeled glass microscope side covered in double-sided tape. This process continued until the contents of each larval home was mounted on a slide in haphazard order (total N=72). The slides were left to dry overnight in a laminar flow hood and then stored under the hood until analysis.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Samples were analyzed using a Thermo-Fisher Element2 inductively coupled plasma mass spectrometer with a Teledyne ATLex 300si-x 193nm Excimer laser ablation unit (LA ICP-MS). To correct for mass bias and instrument drift, National Institute of Technology Standards-certified standards (Reference Material 612, 614, and 616) were run at the beginning and end of every 4 slide sequence (~140 burns). Concentrations of the following elements were quantified from laboratory larval samples: <sup>48</sup>Ca, <sup>55</sup>Mn, <sup>88</sup>Sr, <sup>138</sup>Ba, and <sup>208</sup>Pb; and from field-collected spat: <sup>26</sup>Mg, <sup>48</sup>Ca, <sup>55</sup>Mn, <sup>63</sup>Cu, <sup>88</sup>Sr, <sup>118</sup>Sn,<sup> 138</sup>Ba, and <sup>208</sup>Pb. These elements were all analyzed in low-resolution mode, and were chosen because of their previous use in uptake and tagging studies of fish otoliths and bivalve shells (Bath Martin &amp; Thorrold 2005; Strasser et al. 2008a,b; Fodrie et al. 2011).</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Larval slide-mounts from the laboratory experiment were ablated in bulk using a line transect of 150 μm with 40 μm spot size and 80% laser intensity. Isotope intensities were converted into elemental ratios (X:Ca) following Becker et al. (2007).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1155609 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1155609
completed
F. Joel Fodrie
University of North Carolina at Chapel Hill
252-726-6841
Institute of Marine Sciences & Department of Marine Sciences 3431 Arendell Street
Morehead City
NC
28557
USA
jfodrie@unc.edu
pointOfContact
asNeeded
Unknown
Burn_Number
Burn_Date
Name
Sequence
Mg26_to_Ca48
Mn55_to_Ca48
Co59_to_Ca48
Cu63_to_Ca48
Sr88_to_Ca48
Cd112_to_Ca48
Ba138_to_Ca48
Sn118_to_Ca48
La139_to_Ca48
Ce140_to_Ca48
Pb208_to_Ca48
Temp_Treat
Sal_Treat
Elemental_Conc_Treat
Home
Teledyne ATLex 300si-x 193nm Excimer laser ablation unit
Thermo-Fisher Element2 inductively coupled plasma mass spectrometer
theme
None, User defined
sampling_sequence
date
sample identification
sample description
ratio
treatment
featureType
BCO-DMO Standard Parameters
Laser
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Fodrie_EstuarineMetaDyn
service
Deployment Activity
Back Sound, North Carolina
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Interacting Effects of Local Demography and Larval Connectivity on Estuarine Metapopulation Dynamics
https://osprey.bco-dmo.org/project/559332
Interacting Effects of Local Demography and Larval Connectivity on Estuarine Metapopulation Dynamics
<p>Description from NSF award abstract:<br />
The PIs will use the eastern oyster (<em>Crassostrea virginica</em>) in Pamlico Sound, North Carolina, as a model system and will attempt to optimize the design of networks of no-take reserves as a strategy for maintaining metapopulations of this commercially harvested species. The project specifically recognizes that network persistence depends on (1) the potential for growth, survival, and reproduction within reserves, and (2) the potential to distribute offspring among reserves. Thus, demographic processes within reserves and settling areas play important roles, along with variability of physical transport. The PIs plan to:<br />
(1) test and refine 3D bio-physical models of connectivity due to oyster larval transport in a shallow, wind-dominated system;<br />
(2) test, refine, and apply technology to detect natal origins of larvae using geochemical tags in larval shell; and<br />
(3) integrate regional connectivity and demographic rates to model metapopulation dynamics.</p>
<p>This study will produce new tools and test and refine others used for studying larval connectivity, a fundamentally important process in the maintenance of natural populations, and thus in biological conservation and resource management. The tools include a hydrodynamic modeling tool coupled with an open-source particle tracking model that will be available on-line with computer code and user guide. The project will use integrated modeling approaches to evaluate the design of reserve networks: results will be directly useful to improving oyster and ecosystem-based management in Pamlico Sound, and the methods will inform approaches to network design in other locations.</p>
EstuarineMetaDyn
largerWorkCitation
project
eng; USA
oceans
Back Sound, North Carolina
2018-01-25
North Carolina Estuaries
0
BCO-DMO catalogue of parameters from Elemental ratio data from LA ICP-MS analysis of oyster larvae exposed to various temperature, salinity, and elemental concentration treatments conducted in December of 2013 (EstuarineMetaDyn project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/734304.rdf
Name: Burn_Number
Units: unitless
Description: <p>Number corresponding to the order in which the samples were burned (in a given sequence) during LA ICP-MS analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/734305.rdf
Name: Burn_Date
Units: unitless
Description: <p>Date the sample was run through the mass spectometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/734306.rdf
Name: Name
Units: unitless
Description: <p>Sample ID: ##_X#_X. ## = box sample held in. X# = position in box. X = replicate burn</p>
http://lod.bco-dmo.org/id/dataset-parameter/734307.rdf
Name: Sequence
Units: unitless
Description: <p>Name of folder in which all ICPMS chromatograms are stored in on instrument.</p>
http://lod.bco-dmo.org/id/dataset-parameter/734308.rdf
Name: Mg26_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Mg:Ca). Mg26 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734309.rdf
Name: Mn55_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Mn:Ca). Mn55 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734310.rdf
Name: Co59_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Co:Ca). Co59 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734311.rdf
Name: Cu63_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Cu:Ca). Cu63 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734312.rdf
Name: Sr88_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Sr:Ca). Sr88 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734313.rdf
Name: Cd112_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Cd:Ca). Cd112 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734314.rdf
Name: Ba138_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Ba:Ca). Ba138 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734315.rdf
Name: Sn118_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Sn:Ca). Sn118 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734316.rdf
Name: La139_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (La:Ca). La139 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734317.rdf
Name: Ce140_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Ce:Ca). Ce140 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734318.rdf
Name: Pb208_to_Ca48
Units: dimensionless
Description: <p>Elemental ratio (Pb:Ca). Pb208 isotope intensity in counts per second divided by the Ca48 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/734319.rdf
Name: Temp_Treat
Units: unitless
Description: <p>Temperature treatment (T+ = 26*C; T- = 21*C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/734320.rdf
Name: Sal_Treat
Units: unitless
Description: <p>Salinity treatment (S+ = 20 ppt; S- = 12 ppt)</p>
http://lod.bco-dmo.org/id/dataset-parameter/734321.rdf
Name: Elemental_Conc_Treat
Units: unitless
Description: <p>[++] = 32 ppb Mn and 0.32 ppb Pb spikes; [+] = 16 ppb Mn and 0.16 ppb Mg spikes; [0] = ambient</p>
http://lod.bco-dmo.org/id/dataset-parameter/734322.rdf
Name: Home
Units: unitless
Description: <p>Replicate PVC larval "home" label within each experimental aquaria</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
34840
https://datadocs.bco-dmo.org/file/0AAMNYZS2WZxBz/lablarvae.csv
lablarvae.csv
Primary data file for dataset ID 719172
download
https://osprey.bco-dmo.org/dataset/719172/data/download
download
onLine
dataset
<p>Sampling and Analytical Methodology:&nbsp;</p>
<p>To investigate environmental effects on larval (prodissoconch) shell signatures, we manipulated temperature, salinity, and elemental concentration of the water surrounding developing oyster larvae. Individual tanks were set up with the following treatments: low (21°C) or high (26.5°C) temperature; low (12.5 ppt) or high (20 ppt) salinity; and ambient (no addition), mid spike (+16 ppb Mn/0.16 ppb Pb addition), or elevated spike (+32 ppb Mn/0.32 ppb Pb) in concentrations of aqueous Mn and Pb. Temperature and salinity treatments were selected based on representative high and low observations in Pamlico Sound at the time of the experiment (mid-September). Trace metal spikes were calculated to increase the ambient levels of Mn and Pb in seawater, as measured by Becker et al. (2005), by 400% and 800% for mid and elevated spike levels, respectively.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Three-day old <em>C. virginica</em> larvae were obtained from the University of Maryland’s Horn Point Laboratory in Cambridge, Maryland, USA. Upon arrival at the Institute of Marine sciences (IMS) in Morehead City, NC, larvae were divided equally into 2, 1.2 L aerated holding tanks filled with a 12.5 ppt seawater mix (ultrapure H<sub>2</sub>O added to filtered seawater from Bogue Sound, NC). Over the next 4 days, larvae were acclimatized, with one tank receiving a salinity increase of approximately 2 ppt per day, resulting in a final salinity of 20 ppt, while the other tank remained at 12.5 ppt.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; After the acclimatization process was complete, larvae from both holding tanks, now 7 days old, were divided equally into 72 “larval homes”, with approximately 1.6 x 10<sup>4</sup> larvae per home (21.2 larvae cm<sup>-3</sup>). Larval homes were constructed from hollow PVC tubing capped on each side with nitex cloth, with a 30 μm mesh opening, to allow for the flow of water and food into the home, but prevent larvae from escaping. Homes were then placed into 24 aerated aquarium tanks (35 L), with 3 homes per tank. All tubing, PVC, air stones, and nitex were soaked in a HNO<sub>3</sub> solution and then rinsed thoroughly with ultrapure H<sub>2</sub>O prior to its use in the experiment.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Temperatures were maintained at either high or low level by 150 W Aquatop aquarium heaters and salinity levels were established by mixing filtered seawater with ultrapure H<sub>2</sub>O until desired salinity was reached. Mn and Pb concentrations were spiked by the addition of 545 μl of Mn + 5.45 μl of Pb and 1090 μl of Mn + 10.90 μl of Pb, from1000 ppt Fisher Scientific reference standard solutions, for mid and elevated spike treatments, respectively. Water changes were conducted every other day by removing one-third (~12 L) of water from the tank and replacing it with a freshly made mix. Tanks with mid or elevated spike treatments were then re-spiked to maintain consistent trace element concentrations. Immediately following water changes, larvae were fed by depositing dilute Instant Algae Shellfish Diet 1800 (Reed Mariculture; Campbell, California, USA) into larval homes via syringe. Each treatment group was crossed, to produce a full factorial design with 12 total treatment combinations. The experiment ran for 7 days, until the larvae were 14 days old.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Dissolved oxygen, temperature and salinity were monitored daily with a HACH HQ40d dual input, multi-parameter portable water quality meter. Dissolved oxygen, pH, salinity and temperature measures remained consistent among the treatments throughout our laboratory experiments. Mean dissolved oxygen and pH were 8.68 ± 0.025 mg L<sup>-1</sup> and 7.72 ± 0.032, respectively. Mean salinity for high and low salinity treatments were 20.7 ±0.091 ppt and 12.8± 0.120 ppt, respectively. Mean temperature for high temperature treatments was 25.7 ± 0.157 °C and 21.3 ±0.104 °C for low temperature treatments.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; At the conclusion of these mesocosm incubations, larvae from each home were filtered using nitex cloth (30 μm) and then resuspended in 15 mL of water from their respective tank. A 0.5-1 mL subsample of each larval resuspension was removed and the number of whole larvae were counted. The remaining larval solution was then frozen at -23°C until sample preparation for geochemical analysis.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Frozen larvae were thawed and approximately 1000 larvae were obtained representing each replicate home. The larvae were then rinsed with ultrapure H<sub>2</sub>O and mounted as a concentrated mass on a labeled glass microscope side covered in double-sided tape. This process continued until the contents of each larval home was mounted on a slide in haphazard order (total N=72). The slides were left to dry overnight in a laminar flow hood and then stored under the hood until analysis.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Samples were analyzed using a Thermo-Fisher Element2 inductively coupled plasma mass spectrometer with a Teledyne ATLex 300si-x 193nm Excimer laser ablation unit (LA ICP-MS). To correct for mass bias and instrument drift, National Institute of Technology Standards-certified standards (Reference Material 612, 614, and 616) were run at the beginning and end of every 4 slide sequence (~140 burns). Concentrations of the following elements were quantified from laboratory larval samples: <sup>48</sup>Ca, <sup>55</sup>Mn, <sup>88</sup>Sr, <sup>138</sup>Ba, and <sup>208</sup>Pb; and from field-collected spat: <sup>26</sup>Mg, <sup>48</sup>Ca, <sup>55</sup>Mn, <sup>63</sup>Cu, <sup>88</sup>Sr, <sup>118</sup>Sn,<sup> 138</sup>Ba, and <sup>208</sup>Pb. These elements were all analyzed in low-resolution mode, and were chosen because of their previous use in uptake and tagging studies of fish otoliths and bivalve shells (Bath Martin &amp; Thorrold 2005; Strasser et al. 2008a,b; Fodrie et al. 2011).</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Larval slide-mounts from the laboratory experiment were ablated in bulk using a line transect of 150 μm with 40 μm spot size and 80% laser intensity. Isotope intensities were converted into elemental ratios (X:Ca) following Becker et al. (2007).</p>
Specified by the Principal Investigator(s)
<p>Data Processing:</p>
<p>A 2-way ANOVA was used to test the effects of salinity and temperature on elemental ratios for the elements that were <u>not spiked</u> during the laboratory experiment (Sr and Ba). Due to the large amount of zero values in certain cases (e.g., undetectable amounts of Ba), Sr ratios were transformed using a Box-Cox transformation and Ba ratios were transformed to meet assumptions of normality and homogenous variances. After ensuring no interactive effects of Mn and Pb spikes with Sr or Ba signatures, or nesting effects for homes within individual tanks (p&gt;0.05), all tanks were included in this analysis with individual larval homes treated as replicates (N=6) and temperature and salinity treated as fixed factors.</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For spiked elements (Mn and Pb), a three-way ANOVA was used to test the effects of salinity, temperature, and spike level on elemental ratios. Mn ratios were transformed with a Box-Cox transformation, while Pb ratios were transformed logarithmically to meet assumptions of normality. After ensuring no nesting effects of individual tanks, homes were treated as replicates (N=6) temperature, salinity and spike level were treated as fixed factors. For all 4 elements, Tukey’s HSD tests were used post-hoc to explore differences within and among treatment groups</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Teledyne ATLex 300si-x 193nm Excimer laser ablation unit
Teledyne ATLex 300si-x 193nm Excimer laser ablation unit
PI Supplied Instrument Name: Teledyne ATLex 300si-x 193nm Excimer laser ablation unit PI Supplied Instrument Description:http://www.cetac.com/product_dashboard/laser-ablation.htm Instrument Name: Laser Instrument Short Name: Instrument Description: A device that generates an intense beam of coherent monochromatic light (or other electromagnetic radiation) by stimulated emission of photons from excited atoms or molecules.
Thermo-Fisher Element2 inductively coupled plasma mass spectrometer
Thermo-Fisher Element2 inductively coupled plasma mass spectrometer
PI Supplied Instrument Name: Thermo-Fisher Element2 inductively coupled plasma mass spectrometer PI Supplied Instrument Description:Both larval and spat samples were analyzed using a Thermo-Fisher Element2 inductively coupled plasma mass spectrometer with a Teledyne ATLex 300si-x 193nm Excimer laser ablation unit (LA ICP-MS). To correct for mass bias and instrument drift, National Institute of Technology Standards-certified standards (Reference Material 612, 614, and 616) were run at the beginning and end of every 4 slide sequence (~140 burns). Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Fodrie_EstuarineMetaDyn
Fodrie_EstuarineMetaDyn
Back_Sound_NC
shoreside
Fodrie_EstuarineMetaDyn
F. Joel Fodrie
University of North Carolina at Chapel Hill
Back_Sound_NC
shoreside