http://lod.bco-dmo.org/id/dataset/719420
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-11-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments.
2017-11-14
publication
2017-11-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2017-11-15
publication
https://doi.org/10.1575/1912/bco-dmo.719449
Hannes Baumann
University of Connecticut
principalInvestigator
Janet Nye
Stony Brook University - SoMAS
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Baumann, H., Nye, J. (2017) Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2017-11-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.719449 [access date]
Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments. Dataset Description: <p><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">Wild-caught M.&nbsp;</span></span><span style="color:rgb(0, 0, 0); font-family:times new roman; font-size:12pt"><span style="font-family:arial,helvetica,sans-serif">menidia&nbsp;adults were spawned to test whether offspring from different mothers differ in their average survival and size responses to elevated CO2&nbsp;conditions.&nbsp;The experiment quantified three related survival and three size traits for each replicate, female, and CO2&nbsp;treatment: embryo survival (fertilization to 1&nbsp;dph), larval survival (1 to 16&nbsp;dph), and overall survival (fertilization to 16&nbsp;dph); and size (SL) at hatch (1&nbsp;dph), SL at 16&nbsp;dph</span></span><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">, and larval growth rate&nbsp;(GR = (SL16dph&nbsp;– SL1dph)/15).</span></span></p>
<p><strong>These data are&nbsp;associated with the corresponding paper:</strong></p>
<p><a href="https://doi.org/10.1016/j.jembe.2017.11.002" target="_blank">Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. <em>Journal of Experimental Marine Biology and Ecology</em> (in press).</a></p>
<p><strong>Other datasets related to this paper:</strong></p>
<p><a href="https://www.bco-dmo.org/dataset/719379" target="_blank"><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">Fatty acid profiles of M. menidia females and their unfertilized eggs.</span></a></p> Methods and Sampling: <p>Methodology from<strong>&nbsp;Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. <em>Journal of Experimental Marine Biology and Ecology</em> (in press).</strong></p>
<p>Five randomly selected females were strip-spawned onto cutout sections of window screen (1-mm mesh) that were placed into separate seawater-filled spawning dishes (Murray et al., 2014). To ensure full fertilization success and randomize potential paternal effects, eggs were fertilized with a mixture of milt from 22 males, thus producing full-sib and maternal half-sib embryos from each female. Adults were measured for total length (TL; mean TLmale = 9.14 cm, mean TLfemale = 10.4 cm) and frozen for later analysis of FA. Mesh screens with attached embryos were subsequently cut into smaller sections to allow precise enumeration, and within 2-hr post-fertilization 100 embryos were placed into each of three replicate rearing containers (20 L) per female and CO2 treatment (i.e., 600 embryos for each of five females, 3 × 100 in ambient and 3 × 100 in acidified treatments). Rearing containers were filled with 1-um filtered, UV-sterilized seawater (~30 psu) from Long Island Sound and placed in temperature-controlled water baths set to 24 deg C, the known thermal optimum for survival and growth in this species (Middaugh et al., 1987). Offspring were reared for 24 d post fertilization under a 15h light:9h dark lighting regime. After hatch, larvae were fed ad libitum rations of newly hatched brine shrimp nauplii Artemia salina (brineshrimpdirect.com), and 50% of water was replaced every 5 d to ensure safe ammonia levels (&lt; 0.25 ppm). Hatched larvae were counted and subsampled (n = 10 per replicate) at 1 d post hatch (dph) by gently scooping them into identical 20 L containers, and final samples were taken at 16 dph. All samples were preserved in 5% buffered formalin for later measurements of larval standard length (SL, 0.01 mm) via calibrated digital images (ImagePro Premier, MediaCybernetics). The experiment thus quantified three related survival and three size traits for each replicate, female, and CO2 treatment: embryo survival (fertilization to 1 dph), larval survival (1 to 16 dph), overall survival (fertilization to 16 dph), size (SL) at hatch (1 dph), SL at 16 dph, and larval growth rate (GR = (SL16dph – SL1dph)/15).</p>
<p><strong>CO2 regime:&nbsp;</strong></p>
<p>Offspring were reared at ambient (~ 400 uatm, pHNBS = 8.18) and acidified CO2 conditions (~2,300 uatm, pHNBS = 7.50). The higher value was set to a level commonly used in OA research (consistent with projections of future pCO2 values for open oceans over in the next 200 yr (IPCC, 2007)) and represents current conditions experienced during seasonal extremes by this species in nature (Murray et al., 2014). Ambient conditions were achieved by bubbling partially CO2-stripped air into each rearing container, thereby offsetting metabolic CO2 accumulation. Acidified conditions were achieved via gas proportioners (Cole Parmer®) that mixed CO2 stripped air with 100% bone-dry CO2 delivered to the bottom of each rearing container via air stones. Target pH and temperature were monitored daily via a handheld pH probe (Hach® HQ40d portable meter with a PHC201 standard pH-probe) calibrated regularly via two-point National Bureau of Standards (NBS) pH buffers (electronic supplementary material, Fig.S1). To characterize actual pCO2 levels and related water chemistry parameters, water was sampled from four randomly chosen rearing containers per treatment three times over the course of the experiment and immediately measured for total alkalinity (AT) via endpoint titration (Mettler Toledo™ G20 Potentiometric Titrator). The instrument has previously been shown to quantify AT in Dr. Andrew Dickson’s reference material (batch 147, AT= 2231.39 umol kg seawater-1) with an average error of 0.6%. Actual levels of total dissolved inorganic carbon (CT), partial pressure of CO2 (pCO2), fugacity of CO2 (fCO2), and carbonate ion concentration were calculated in CO2SYS (http://cdiac.ornl.gov/ftp/co2sys) based on measured AT, pH (NBS), temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987) and Dickson (1990) for KHSO4 (Table 1).<strong> </strong></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536336
completed
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Janet Nye
Stony Brook University - SoMAS
252-726-6841 ext 158
Institute of Marine Sciences 3431 Arendell St.
Morehead City
NC
28557
USA
jnye@email.unc.edu
pointOfContact
asNeeded
Dataset Version: Final
Unknown
Species
Collection_site
Lat
Lon
Collection_date
Female
Total_length
pH
pCO2
Replicate
Embryo_survival
Larval_survival
Overall_survival
Hatch_length
Larval_length
Growth
Hach® HQ40d portable meter with a PHC201 standard pH-probe
Mettler Toledo™ G20 Potentiometric Titrator
theme
None, User defined
species scientific name (binomial)
site
latitude
longitude
date
sample identification
No BCO-DMO term
pH
Partial pressure of CO2
replicate
length
growth
featureType
BCO-DMO Standard Parameters
pH Sensor
Titrator
instrument
BCO-DMO Standard Instruments
AP_Rankin
service
Deployment Activity
eastern Long Island Sound
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
https://osprey.bco-dmo.org/project/650184
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
<p><em>Description from NSF award abstract:</em><br />
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.</p>
<p>This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.</p>
<p><strong>Related references:</strong><br />
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:<a href="http://dx.doi.org/10.1098/rsbl.2015.0976" target="_blank">10.1098/rsbl.2015.0976</a></p>
<p>Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:<a href="http://dx.doi.org/10.1002/loe2.10002" target="_blank">10.1002/loe2.10002</a></p>
<p>Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:<a href="http://dx.doi.org/10.3354/meps11142" target="_blank">10.3354/meps11142</a></p>
HYPOA
largerWorkCitation
project
eng; USA
oceans
eastern Long Island Sound
-72.0148361
-72.0148361
41.3213389
41.3213389
2017-11-14
Eastern Long Island Sound, CT, USA
0
BCO-DMO catalogue of parameters from Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/719432.rdf
Name: Species
Units: unitless
Description: <p>Atlantic silverside; Menidia menidia</p>
http://lod.bco-dmo.org/id/dataset-parameter/719433.rdf
Name: Collection_site
Units: unitless
Description: <p>Mumford Cove Connecticut USA</p>
http://lod.bco-dmo.org/id/dataset-parameter/719434.rdf
Name: Lat
Units: decimal degrees
Description: <p>Latitude of field collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/719435.rdf
Name: Lon
Units: decimal degrees
Description: <p>Longitude of field collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/719436.rdf
Name: Collection_date
Units: unitless
Description: <p>Date of field collection for the spawners used in the experiment; YYYY/MM/DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/719437.rdf
Name: Female
Units: unitless
Description: <p>Five females denoted by letter A B C D E</p>
http://lod.bco-dmo.org/id/dataset-parameter/719438.rdf
Name: Total_length
Units: centimeters
Description: <p>Fish were measured to the lower half centimeter; lower 0.5 centimeters</p>
http://lod.bco-dmo.org/id/dataset-parameter/719439.rdf
Name: pH
Units: NIST units
Description: <p>pH units reported on the NIST scale</p>
http://lod.bco-dmo.org/id/dataset-parameter/719440.rdf
Name: pCO2
Units: uatm
Description: <p>Calculated CO2 partial pressure</p>
http://lod.bco-dmo.org/id/dataset-parameter/719441.rdf
Name: Replicate
Units: unitless
Description: <p>There were 3 replicates labled 1 2 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/719442.rdf
Name: Embryo_survival
Units: percent
Description: <p>Relative survival of offspring from fertilization to 1 day post hatch</p>
http://lod.bco-dmo.org/id/dataset-parameter/719443.rdf
Name: Larval_survival
Units: percent
Description: <p>Relative survival of offspring from data 1 to day 16 post hatch</p>
http://lod.bco-dmo.org/id/dataset-parameter/719444.rdf
Name: Overall_survival
Units: percent
Description: <p>Relative survival of offspring from fertilization to day 16 post hatch</p>
http://lod.bco-dmo.org/id/dataset-parameter/719445.rdf
Name: Hatch_length
Units: millimeters
Description: <p>Mean length of newly hatched larvae per replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/719446.rdf
Name: Larval_length
Units: millimeters
Description: <p>Mean length of larvae at the end of the experiment per replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/719447.rdf
Name: Growth
Units: millimeters per day
Description: <p>Average growth of larvae from d1 to d16 per replicate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3479
https://darchive.mblwhoilibrary.org/bitstream/1912/9366/1/data_survival-and-size.tsv
download
https://doi.org/10.1575/1912/bco-dmo.719449
download
onLine
dataset
<p>Methodology from<strong>&nbsp;Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. <em>Journal of Experimental Marine Biology and Ecology</em> (in press).</strong></p>
<p>Five randomly selected females were strip-spawned onto cutout sections of window screen (1-mm mesh) that were placed into separate seawater-filled spawning dishes (Murray et al., 2014). To ensure full fertilization success and randomize potential paternal effects, eggs were fertilized with a mixture of milt from 22 males, thus producing full-sib and maternal half-sib embryos from each female. Adults were measured for total length (TL; mean TLmale = 9.14 cm, mean TLfemale = 10.4 cm) and frozen for later analysis of FA. Mesh screens with attached embryos were subsequently cut into smaller sections to allow precise enumeration, and within 2-hr post-fertilization 100 embryos were placed into each of three replicate rearing containers (20 L) per female and CO2 treatment (i.e., 600 embryos for each of five females, 3 × 100 in ambient and 3 × 100 in acidified treatments). Rearing containers were filled with 1-um filtered, UV-sterilized seawater (~30 psu) from Long Island Sound and placed in temperature-controlled water baths set to 24 deg C, the known thermal optimum for survival and growth in this species (Middaugh et al., 1987). Offspring were reared for 24 d post fertilization under a 15h light:9h dark lighting regime. After hatch, larvae were fed ad libitum rations of newly hatched brine shrimp nauplii Artemia salina (brineshrimpdirect.com), and 50% of water was replaced every 5 d to ensure safe ammonia levels (&lt; 0.25 ppm). Hatched larvae were counted and subsampled (n = 10 per replicate) at 1 d post hatch (dph) by gently scooping them into identical 20 L containers, and final samples were taken at 16 dph. All samples were preserved in 5% buffered formalin for later measurements of larval standard length (SL, 0.01 mm) via calibrated digital images (ImagePro Premier, MediaCybernetics). The experiment thus quantified three related survival and three size traits for each replicate, female, and CO2 treatment: embryo survival (fertilization to 1 dph), larval survival (1 to 16 dph), overall survival (fertilization to 16 dph), size (SL) at hatch (1 dph), SL at 16 dph, and larval growth rate (GR = (SL16dph – SL1dph)/15).</p>
<p><strong>CO2 regime:&nbsp;</strong></p>
<p>Offspring were reared at ambient (~ 400 uatm, pHNBS = 8.18) and acidified CO2 conditions (~2,300 uatm, pHNBS = 7.50). The higher value was set to a level commonly used in OA research (consistent with projections of future pCO2 values for open oceans over in the next 200 yr (IPCC, 2007)) and represents current conditions experienced during seasonal extremes by this species in nature (Murray et al., 2014). Ambient conditions were achieved by bubbling partially CO2-stripped air into each rearing container, thereby offsetting metabolic CO2 accumulation. Acidified conditions were achieved via gas proportioners (Cole Parmer®) that mixed CO2 stripped air with 100% bone-dry CO2 delivered to the bottom of each rearing container via air stones. Target pH and temperature were monitored daily via a handheld pH probe (Hach® HQ40d portable meter with a PHC201 standard pH-probe) calibrated regularly via two-point National Bureau of Standards (NBS) pH buffers (electronic supplementary material, Fig.S1). To characterize actual pCO2 levels and related water chemistry parameters, water was sampled from four randomly chosen rearing containers per treatment three times over the course of the experiment and immediately measured for total alkalinity (AT) via endpoint titration (Mettler Toledo™ G20 Potentiometric Titrator). The instrument has previously been shown to quantify AT in Dr. Andrew Dickson’s reference material (batch 147, AT= 2231.39 umol kg seawater-1) with an average error of 0.6%. Actual levels of total dissolved inorganic carbon (CT), partial pressure of CO2 (pCO2), fugacity of CO2 (fCO2), and carbonate ion concentration were calculated in CO2SYS (http://cdiac.ornl.gov/ftp/co2sys) based on measured AT, pH (NBS), temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987) and Dickson (1990) for KHSO4 (Table 1).<strong> </strong></p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong><br />
- added underscores to column headers<br />
- replaced blank cells with nd<br />
- added underscores to site name<br />
- removed commas from data<br />
- changed date format to yyyy/mm/dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hach® HQ40d portable meter with a PHC201 standard pH-probe
Hach® HQ40d portable meter with a PHC201 standard pH-probe
PI Supplied Instrument Name: Hach® HQ40d portable meter with a PHC201 standard pH-probe PI Supplied Instrument Description:handheld pH probe Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Mettler Toledo™ G20 Potentiometric Titrator
Mettler Toledo™ G20 Potentiometric Titrator
PI Supplied Instrument Name: Mettler Toledo™ G20 Potentiometric Titrator PI Supplied Instrument Description:Used to measure total alkalinity Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: AP_Rankin
AP_Rankin
Avery_Point
Avery_Point
laboratory
Avery_Point
Avery_Point
laboratory