http://lod.bco-dmo.org/id/dataset/719459
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-11-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Size of Bugula neritina colonies with and without symbiont grown at different temperatures in laboratory mesocosms at Georgia Tech
2017-11-16
publication
2017-11-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-01
publication
https://doi.org/10.1575/1912/bco-dmo.719459.1
Nicole Lopanik
Georgia Institute of Technology
principalInvestigator
Grace Lim-Fong
Randolph-Macon College
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lopanik, N., Lim-Fong, G. (2017) Size of Bugula neritina colonies with and without symbiont grown at different temperatures in laboratory mesocosms at Georgia Tech. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-11-16 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.719459.1 [access date]
Size of Bugula neritina colonies with and without symbiont grown at different temperatures Dataset Description: <p>Size of <em>Bugula neritina</em> colonies with and without symbiont grown at different temperatures in laboratory mesocosms.</p> Methods and Sampling: <p><em>Bugula neritina</em> colonies were collected by hand off the sides of floating docks around Morehead City, NC (latitude: 34.7, longitude: -76.8). Larvae from individual colonies were collected and settled into 10 mL of filtered seawater with (treated) or without (control) 100 ug/mL of gentamicin.&nbsp;Larvae were allowed to settle overnight. The control and treated seawater was changed the next morning for the next 3 days. The plates were transported to Georgia Tech where they were placed in 2.5 gallon tanks with artificial seawater maintained in environmental chambers at 16 degrees C and 22 degrees C. They were fed <em>ad libitum</em> an artificial diet of the phytoplankton <em>Rhodomonas lens</em> for approximately one month.&nbsp;After the 1-month period, the size of the colonies was measured by counting the number of bifurcations in each colony.&nbsp;A Leica stereomicroscope was used while counting the number of colony bifurcations. Samples of larvae, juvenile, and the grown colonies were placed in RNAlater for symbiont quantification analysis by quantitative PCR (currently in progress).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1608709 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1608709
completed
Nicole Lopanik
Georgia Institute of Technology
404-894-1754
EAS 311 Ferst Drive
Atlanta
GA
30332
USA
nicole.lopanik@eas.gatech.edu
pointOfContact
Grace Lim-Fong
Randolph-Macon College
804-752-3725
304 Caroline St.
Ashland
VA
23005
USA
glim@rmc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
temperature
colony_num
genotype
cont_avg
cont_sd
cont_N
treat_avg
treat_sd
treat_N
tank
Leica stereomicroscope
theme
None, User defined
water temperature at measurement depth
sample identification
sample description
count
featureType
BCO-DMO Standard Parameters
Aquarium
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Biogeography of a marine defensive microbial symbiont: relative importance of host defense vs. abiotic factors
https://osprey.bco-dmo.org/project/645348
Biogeography of a marine defensive microbial symbiont: relative importance of host defense vs. abiotic factors
<p>Recent research has shown that microorganisms can be very important to their eukaryotic hosts, by providing nutrition or contributing to host defense against enemies, such as pathogens or predators. In many cases, however, hosting a bacterial symbiont imposes a physiological cost on the host organism, resulting in reduced growth or reproduction in the presence of the symbiont. Further, these costs may be more pronounced in some habitats than others, causing natural selection to act in eliminating symbiont-containing hosts from the population. In this project, the investigators are studying the relationship between the marine bryozoan invertebrate, Bugula neritina, and its uncultured symbiont. The symbiont produces natural products with activity against cancer, Alzheimer's disease, and HIV. Interestingly, these compounds also are distasteful and protect larvae from predators, indicating that this symbiotic relationship is defensive in nature. Along the East Coast of the US, the investigators have found a much higher proportion of individuals that have the defensive symbiont at lower latitudes, while the symbiont is absent in individuals collected at higher latitudes. This pattern is consistent with the theory that higher predation pressure exists at lower latitudes. Other environmental factors, such as temperature, can also vary over a wide geographical area, and may also play a role in influencing the relationship. In this project, the investigators will evaluate the ecological and environmental parameters that influence the distribution of a defensive symbiont, including predation pressure and temperature. Defensive symbionts represent another level of ecological complexity, and likely play an important role in structuring marine communities. This study will provide insight into how environmental factors can influence host-symbiont interactions and drive partner co-evolution. Furthermore, the bioactive products have pharmaceutical potential, and understanding how environmental factors influence the relationship between B. neritina and its symbiont may improve bioprospecting for novel compounds that could be developed into drugs.</p>
<p>
In this research, the investigators will determine the ecological and environmental parameters that influence the distribution of a defensive symbiont in the marine bryozoan, Bugula neritina. The goal of this research is to determine the mechanism that results in the defensive endosymbiont being restricted to hosts that inhabit lower latitudes. This pattern of symbiont distribution could be the result of differing levels of costs and benefits at different latitudes: where predation pressure is low, the costs of hosting the symbiont outweigh the benefits, and aposymbiotic individuals outcompete their symbiotic conspecifics. In areas of higher predation, the defensive benefit outweighs the cost, and symbiotic individuals have higher survival rates than their undefended, aposymbiotic conspecifics. An alternative, but not mutually exclusive hypothesis, is that symbiont growth is inhibited at higher latitudes, where it is not as beneficial, and growth is induced in areas of higher predation. Specific goals are to determine if (1) a biogeographical cline in predation pressure corresponds to a gradient of symbiont frequency associating with the host, (2) symbiotic hosts have a higher fitness at low latitudes, and aposymbiotic hosts have a higher fitness at high latitudes, and (3) symbiont growth is promoted at low latitudes and inhibited at high latitudes. A combination of field and laboratory-based experiments will be conducted using ecological and molecular biology techniques. Bioactive compounds produced by symbionts of marine invertebrates can mediate multi-trophic interactions and potentially influence benthic community structure. There has been almost no research, however, on how ecological and environmental parameters influence the distribution of marine defensive endosymbionts.</p>
<p>Related Reference:</p>
<p>Linneman J, Paulus D, Lim-Fong G, Lopanik NB (2014) Latitudinal Variation of a Defensive Symbiosis in the Bugula neritina (Bryozoa) Sibling Species Complex. PLoS ONE 9(10): e108783. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0108783" target="_blank">10.1371/journal.pone.0108783</a></p>
BiogeogDefensiveSymb
largerWorkCitation
project
eng; USA
biota
oceans
-76.8
-76.8
34.7
34.7
2017-11-16
Western Atlantic coast, ranging from latitudes 38.61283 to 29.753272
0
BCO-DMO catalogue of parameters from Size of Bugula neritina colonies with and without symbiont grown at different temperatures in laboratory mesocosms at Georgia Tech
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/719469.rdf
Name: temperature
Units: degrees Celsius
Description: <p>Temperature in microcosm</p>
http://lod.bco-dmo.org/id/dataset-parameter/719470.rdf
Name: colony_num
Units: unitless
Description: <p>Specific colony identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/719471.rdf
Name: genotype
Units: unitless
Description: <p>Host genotype/symbiont presence: S+ means Type S host bryozoan (S), with the symbiont (+, as opposed to -); N+ is Type N host (N), with the symbiont (+).</p>
http://lod.bco-dmo.org/id/dataset-parameter/719472.rdf
Name: cont_avg
Units: unitless
Description: <p>Average size in bifurcations in control colonies</p>
http://lod.bco-dmo.org/id/dataset-parameter/719473.rdf
Name: cont_sd
Units: unitless
Description: <p>Standard deviation of average size in bifurcations in control colonies</p>
http://lod.bco-dmo.org/id/dataset-parameter/719474.rdf
Name: cont_N
Units: unitless
Description: <p>Number of control colonies in which size was measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/719475.rdf
Name: treat_avg
Units: unitless
Description: <p>Average size in bifurcations in antibiotic treated colonies</p>
http://lod.bco-dmo.org/id/dataset-parameter/719476.rdf
Name: treat_sd
Units: unitless
Description: <p>Standard deviation of average size in bifurcations in treated colonies</p>
http://lod.bco-dmo.org/id/dataset-parameter/719477.rdf
Name: treat_N
Units: unitless
Description: <p>Number of treated colonies in which size was measured</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
648
https://darchive.mblwhoilibrary.org/bitstream/1912/24391/1/dataset-719459_mesocosm-growth-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.719459.1
download
onLine
dataset
<p><em>Bugula neritina</em> colonies were collected by hand off the sides of floating docks around Morehead City, NC (latitude: 34.7, longitude: -76.8). Larvae from individual colonies were collected and settled into 10 mL of filtered seawater with (treated) or without (control) 100 ug/mL of gentamicin.&nbsp;Larvae were allowed to settle overnight. The control and treated seawater was changed the next morning for the next 3 days. The plates were transported to Georgia Tech where they were placed in 2.5 gallon tanks with artificial seawater maintained in environmental chambers at 16 degrees C and 22 degrees C. They were fed <em>ad libitum</em> an artificial diet of the phytoplankton <em>Rhodomonas lens</em> for approximately one month.&nbsp;After the 1-month period, the size of the colonies was measured by counting the number of bifurcations in each colony.&nbsp;A Leica stereomicroscope was used while counting the number of colony bifurcations. Samples of larvae, juvenile, and the grown colonies were placed in RNAlater for symbiont quantification analysis by quantitative PCR (currently in progress).</p>
Specified by the Principal Investigator(s)
<p>Data were&nbsp;analyzed using IBM SPSS Statistics 24.</p>
<p>BCO-DMO Processing:<br />
- created a column for temperature;<br />
- combined the two tables into one;<br />
-modified parameter names.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
tank
tank
PI Supplied Instrument Name: tank Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
Leica stereomicroscope
Leica stereomicroscope
PI Supplied Instrument Name: Leica stereomicroscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/