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            <gco:CharacterString>Cite this dataset as: Andersson, A., Bates, N. R. (2017) Carbonate chemistry and light data from bottle samples. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-11-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/719535 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;Surface seawater samples were collected approximately monthly and analyzed for carbonate chemistry and nutrients.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;These data were utilized in the following publication:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;span style=&amp;quot;color:rgb(34, 34, 34); font-family:arial,sans-serif&amp;quot;&amp;gt;Courtney, T. A., Lebrato, M., Bates, N. R., Collins, A., de Putron, S. J., Garley, R., ... &amp;amp;amp; Andersson, A. J. (2017). Environmental controls on modern scleractinian coral and reef-scale calcification.&amp;amp;nbsp;&amp;lt;/span&amp;gt;&amp;lt;em&amp;gt;Science advances&amp;lt;/em&amp;gt;&amp;lt;span style=&amp;quot;color:rgb(34, 34, 34); font-family:arial,sans-serif&amp;quot;&amp;gt;,&amp;amp;nbsp;&amp;lt;/span&amp;gt;&amp;lt;em&amp;gt;3&amp;lt;/em&amp;gt;&amp;lt;span style=&amp;quot;color:rgb(34, 34, 34); font-family:arial,sans-serif&amp;quot;&amp;gt;(11), e1701356.&amp;amp;nbsp;&amp;lt;/span&amp;gt;&amp;lt;span style=&amp;quot;font-size:13.3333px&amp;quot;&amp;gt;doi:&amp;amp;nbsp;&amp;lt;a href=&amp;quot;http://doi.org/10.1126/sciadv.1701356&amp;quot; style=&amp;quot;color: rgb(17, 85, 204);&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1126/sciadv.1701356&amp;lt;/a&amp;gt;&amp;lt;/span&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;At each sample site, a 5 liter Niskin bottle was deployed at 0.5-1.0 m depth according to best practices. Samples for DIC and TA were collected in 200 mL Kimax glass sample bottles, and immediately&amp;amp;nbsp;fixed using 100 µL saturated solution of HgCl2. TA samples were analyzed via closed cell potentiometric titrations using a VINDTA 3S system and DIC was analyzed using coulometric methods on a VINDTA 3c or an infrared based analysis on an AIRICA system. Accuracy and precision of TA and DIC measurements were evaluated by certified reference materials provided by the laboratory of Prof. A. Dickson at Scripps Institution of Oceanography yielding typical precision and accuracy of ±1–2 µmol/kg. A YSI Handheld Multiparameter Instrument was used to measure in situ temperature (±0.15°C), salinity (±1%), dissolved oxygen (±2 %), and percent saturation dissolved oxygen (±2%). Nutrient samples were filtered upon sampling using a 0.4 µM filter and frozen in opaque plastic bottles until processing at Woods Hole Oceanographic Institution Nutrient Analytical Facility using agency methods of analysis for ammonium (detection limit 0.034 µmol/L, method G-172-96), nitrite+nitrate (detection limit 0.010 µmol/L, method G-177-96), silicate (detection limit 0.016 µmol/L, method G-177-96), and phosphate (detection limit 0.025 µmol/L, method G-297-03).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sampling locations: Bermuda Platform: Hog Reef (32.45733, -64.83476) and Crescent Reef (32.40078, -64.79747).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54811.rdf" xlink:title="OCE-0928406" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0928406 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0928406</gmx:Anchor>
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&lt;p&gt;Ocean acidification owing to anthropogenic emission of CO2 is a significant and imminent threat to marine calcifying organisms and ecosystems such as corals and coral reefs. As a result of future ocean acidification, i.e., increasing seawater CO2, and decreasing pH, carbonate ion concentration [CO3], and carbonate saturation state, it is likely that marine calcifiers will have difficulty growing their shells and skeletons of calcium carbonate (CaCO3) at their present rates. Dissolution of carbonate sediments and structures are also likely to increase, and could ultimately exceed calcification and CaCO3 production, leading to a transition from net accumulation to a net loss in carbonate material of individual coral colonies, coral communities and coral reef ecosystems. Because of Bermuda's relatively high-latitude location (32° N), the annual average surface seawater [CO3] is lower in Bermuda than regions closer to the tropics. As a consequence, the Bermuda coral reef is likely to experience critical [CO3] values and net dissolution before its tropical counterparts as a result of continued ocean acidification. Furthermore, a natural gradient in [CO3] exists along the Bermuda reef with environmental parameters such as, light, temperature, and nutrients being near identical. This gradient allows for unique cross-comparisons of calcification of individual calcifiers and calcifying communities under different [CO3] in a natural environment. &lt;/p&gt;
&lt;p&gt;In this study, researchers at the Bermuda Institute of Ocean Science (BIOS) will launch the BEACON project to further our understanding of the consequences of ocean acidification to the process of calcification and CaCO3 production at three different spatial scales including (1) individual coral colonies, (2) local reef communities, and (3) regional coral reef ecosystems. They will conduct (1) in situ and in vitro experiments to assess growth and evaluate net calcification of individual coral colonies of three different species common to Bermuda and the Caribbean exposed to different [CO3] under both natural and controlled experimental conditions; (2) diel and quasi-lagrangian calcification experiments to evaluate net calcification of local reef communities and in moving water masses along the natural [CO3] gradient existent on the Bermuda platform; and (3) time series data collected across the Bermuda platform and offshore, to evaluate net calcification and CaCO3 production of the Bermuda coral reef ecosystem and platform over seasonal and annual cycles. &lt;/p&gt;
&lt;p&gt;Broader impacts: This project will provide fundamental data on the consequences of ocean acidification to coral reefs on different temporal and spatial scales. Combined, knowledge at each of the scales will contribute to an improved understanding of this problem in a broader context, i.e., the effect on coral reefs as a global entity and role in the global carbon cycle during past, present and future seawater chemical conditions. As the meaning implies, the research team envisions BEACON to serve as a guiding light to assist researchers and policymakers in framing future strategies and making decisions regarding the management of coral reefs and CO2 emission policies in order to establish CO2 stabilization targets. Scientific understanding and research products from the project will be specifically used in the BIOS explorer program, CoE POGO ocean acidification module, and the coral reef ecology class taught at BIOS. It will also contribute to developing the research and technical skills of one graduate student and one research technician at BIOS, and will include the involvement of interns and NSF REU fellowship students each year of the project.&lt;/p&gt;
&lt;p&gt;Based on the findings of the BEACON project, and especially the results published in Andersson et al. (Nature Climate Change, 4, 56-61, 2014) and Yeakel et al. (PNAS, 112, 14512-14517, 2015), BEACON II (&lt;a href=&quot;https://www.bco-dmo.org/project/737955&quot;&gt;https://www.bco-dmo.org/project/737955&lt;/a&gt;) aims to assess the links between offshore and reef biogeochemistry by continuing and expanding on the physical and chemical measurements on the Bermuda coral reef and in the surrounding Sargasso Sea.&lt;/p&gt;</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/686.rdf" xlink:title="MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser" xlink:actuate="onRequest">VINDTA 3c (Marianda Inc.)</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>VINDTA 3c (Marianda Inc.)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: VINDTA 3c (Marianda Inc.) PI Supplied Instrument Description:DIC was analyzed using coulometric methods on a VINDTA 3c or an infrared-based analysis on an AIRICA system.
The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric determination of total dissolved inorganic carbon. The instrument analyzes alkalinity and dissolved inorganic carbon content of a sample in parallel. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically ±1 µmol/kg for TA. DIC is analyzed with a UIC coulometer. Please see http://marianda.com/index.php?site=products&amp;subsite=vindta3c&gt; for a complete instrument description. Instrument Name: MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser Instrument Short Name:inorganic carbon and alkalinity analyser   Instrument Description: The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/413.rdf" xlink:title="Niskin bottle" xlink:actuate="onRequest">Niskin Bottle</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Niskin Bottle</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Niskin Bottle PI Supplied Instrument Description:From 2007 to 2012, surface seawater samples from the Bermuda coral reef platform were collected once a month at 0.5–1-m depth using a 5-L Niskin bottle. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle   Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/682.rdf" xlink:title="Titrator" xlink:actuate="onRequest">MARIANDA VINDTA 3S</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
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            <gco:CharacterString>MARIANDA VINDTA 3S</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: MARIANDA VINDTA 3S PI Supplied Instrument Description:Total alkalinity (TA) samples were analyzed via closed cell potentiometric titrations using a VINDTA 3S system. VINDTA (Versatile INstrument for the Determination of Titration Alkalinity). The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA. Please see  for a complete instrument description. Instrument Name: Titrator Instrument Short Name:Titrator   Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/678.rdf" xlink:title="Water Quality Multiprobe" xlink:actuate="onRequest">YSI 556 Handheld Multiparameter Instrument</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>YSI 556 Handheld Multiparameter Instrument</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: YSI 556 Handheld Multiparameter Instrument PI Supplied Instrument Description:A YSI 556 Handheld Multiparameter Instrument was used to measure in situ temperature (accuracy ± 0.15°C), salinity (accuracy ± 1%), DO_mg (accuracy ± 2%), and DO_% (accuracy ± 2%). Instrument Name: Water Quality Multiprobe Instrument Short Name:Water Quality Multiprobe   Instrument Description: An instrument which measures multiple water quality parameters based on the sensor configuration.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: BIOS_BEACON</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/626096.rdf" xlink:title="Cruise" xlink:actuate="onRequest">BIOS_BEACON</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/626095.rdf"
           xlink:actuate="onRequest">BIOS_Small_Boat</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/626095.rdf" xlink:title="BIOS_Small_Boat" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/626096.rdf" xlink:title="Cruise" xlink:actuate="onRequest">BIOS_BEACON</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
                    <gmd:citedResponsibleParty>
                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51444.rdf" xlink:actuate="onRequest">Andreas Andersson</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/26.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">University of California-San Diego Scripps</gmx:Anchor>
                        </gmd:organisationName>
                        <gmd:role>
                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
                      </gmd:role>
                      </gmd:CI_ResponsibleParty>
                    </gmd:citedResponsibleParty>
                   </gmd:CI_Citation>
                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:platform>
  <gmi:MI_Platform>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/626095.rdf"
           xlink:actuate="onRequest">BIOS_Small_Boat</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/626095.rdf" xlink:title="BIOS_Small_Boat" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
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