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                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/645348.rdf" xlink:title="Project" xlink:actuate="onRequest">Biogeography of a marine defensive microbial symbiont: relative importance of host defense vs. abiotic factors</gmx:Anchor>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/645348.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Biogeography of a marine defensive microbial symbiont: relative importance of host defense vs. abiotic factors</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;Recent research has shown that microorganisms can be very important to their eukaryotic hosts, by providing nutrition or contributing to host defense against enemies, such as pathogens or predators. In many cases, however, hosting a bacterial symbiont imposes a physiological cost on the host organism, resulting in reduced growth or reproduction in the presence of the symbiont. Further, these costs may be more pronounced in some habitats than others, causing natural selection to act in eliminating symbiont-containing hosts from the population. In this project, the investigators are studying the relationship between the marine bryozoan invertebrate, Bugula neritina, and its uncultured symbiont. The symbiont produces natural products with activity against cancer, Alzheimer's disease, and HIV. Interestingly, these compounds also are distasteful and protect larvae from predators, indicating that this symbiotic relationship is defensive in nature. Along the East Coast of the US, the investigators have found a much higher proportion of individuals that have the defensive symbiont at lower latitudes, while the symbiont is absent in individuals collected at higher latitudes. This pattern is consistent with the theory that higher predation pressure exists at lower latitudes. Other environmental factors, such as temperature, can also vary over a wide geographical area, and may also play a role in influencing the relationship. In this project, the investigators will evaluate the ecological and environmental parameters that influence the distribution of a defensive symbiont, including predation pressure and temperature. Defensive symbionts represent another level of ecological complexity, and likely play an important role in structuring marine communities. This study will provide insight into how environmental factors can influence host-symbiont interactions and drive partner co-evolution. Furthermore, the bioactive products have pharmaceutical potential, and understanding how environmental factors influence the relationship between B. neritina and its symbiont may improve bioprospecting for novel compounds that could be developed into drugs.&lt;/p&gt;
&lt;p&gt;
In this research, the investigators will determine the ecological and environmental parameters that influence the distribution of a defensive symbiont in the marine bryozoan, Bugula neritina. The goal of this research is to determine the mechanism that results in the defensive endosymbiont being restricted to hosts that inhabit lower latitudes. This pattern of symbiont distribution could be the result of differing levels of costs and benefits at different latitudes: where predation pressure is low, the costs of hosting the symbiont outweigh the benefits, and aposymbiotic individuals outcompete their symbiotic conspecifics. In areas of higher predation, the defensive benefit outweighs the cost, and symbiotic individuals have higher survival rates than their undefended, aposymbiotic conspecifics. An alternative, but not mutually exclusive hypothesis, is that symbiont growth is inhibited at higher latitudes, where it is not as beneficial, and growth is induced in areas of higher predation. Specific goals are to determine if (1) a biogeographical cline in predation pressure corresponds to a gradient of symbiont frequency associating with the host, (2) symbiotic hosts have a higher fitness at low latitudes, and aposymbiotic hosts have a higher fitness at high latitudes, and (3) symbiont growth is promoted at low latitudes and inhibited at high latitudes. A combination of field and laboratory-based experiments will be conducted using ecological and molecular biology techniques. Bioactive compounds produced by symbionts of marine invertebrates can mediate multi-trophic interactions and potentially influence benthic community structure. There has been almost no research, however, on how ecological and environmental parameters influence the distribution of marine defensive endosymbionts.&lt;/p&gt;
&lt;p&gt;Related Reference:&lt;/p&gt;
&lt;p&gt;Linneman J, Paulus D, Lim-Fong G, Lopanik NB (2014) Latitudinal Variation of a Defensive Symbiosis in the Bugula neritina (Bryozoa) Sibling Species Complex. PLoS ONE 9(10): e108783. doi:&lt;a href=&quot;http://dx.doi.org/10.1371/journal.pone.0108783&quot; target=&quot;_blank&quot;&gt;10.1371/journal.pone.0108783&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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        <gmd:MD_TopicCategoryCode>biota</gmd:MD_TopicCategoryCode>
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        <gmd:MD_TopicCategoryCode>oceans</gmd:MD_TopicCategoryCode>
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                      <gco:CharacterString>Western Atlantic coast, ranging from latitudes 38.61283 to 29.753272</gco:CharacterString>
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            <gco:CharacterString>BCO-DMO catalogue of parameters from Distribution of benthic invertebrates along the East Coast, USA, ranging from latitudes 38.61283 to 29.753272.</gco:CharacterString>
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    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
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            http://lod.bco-dmo.org/id/dataset-parameter/719566.rdf
	Name: date
	Units: unitless
	Description: &lt;p&gt;Date of collection, formatted as mm/dd/yyyy&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719567.rdf
	Name: location
	Units: unitless
	Description: &lt;p&gt;Location samples collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719568.rdf
	Name: location_descrip
	Units: unitless
	Description: &lt;p&gt;Specific area within location where samples were collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719569.rdf
	Name: location_code
	Units: unitless
	Description: &lt;p&gt;Location code&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719570.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719571.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude of location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719572.rdf
	Name: temp
	Units: degrees Celsius
	Description: &lt;p&gt;Water temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719573.rdf
	Name: salinity
	Units: parts per thousand
	Description: &lt;p&gt;Water salinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719574.rdf
	Name: grid_num
	Units: unitless
	Description: &lt;p&gt;Sampling effort replicate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719575.rdf
	Name: sponge_random
	Units: unitless
	Description: &lt;p&gt;Random sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719576.rdf
	Name: sponge_orange
	Units: unitless
	Description: &lt;p&gt;Orange sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719577.rdf
	Name: sponge_yellow
	Units: unitless
	Description: &lt;p&gt;Yellow sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719578.rdf
	Name: sponge_purp_gray
	Units: unitless
	Description: &lt;p&gt;Purple/gray sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719579.rdf
	Name: sponge_pink
	Units: unitless
	Description: &lt;p&gt;Pink sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719580.rdf
	Name: sponge_green
	Units: unitless
	Description: &lt;p&gt;Green sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719581.rdf
	Name: spong_orange_encr
	Units: unitless
	Description: &lt;p&gt;Orange encr. sponge count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719582.rdf
	Name: Anemone
	Units: unitless
	Description: &lt;p&gt;Anemone count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719583.rdf
	Name: hydroid
	Units: unitless
	Description: &lt;p&gt;Hydroid count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719584.rdf
	Name: hydroid_tubularia
	Units: unitless
	Description: &lt;p&gt;Hydroid (tubularia) count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719585.rdf
	Name: crab
	Units: unitless
	Description: &lt;p&gt;Crab count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719586.rdf
	Name: barnacle
	Units: unitless
	Description: &lt;p&gt;Barnacle count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719587.rdf
	Name: oyster
	Units: unitless
	Description: &lt;p&gt;Oyster count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719588.rdf
	Name: mussel
	Units: unitless
	Description: &lt;p&gt;Mussel count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719589.rdf
	Name: snail
	Units: unitless
	Description: &lt;p&gt;Snail count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719590.rdf
	Name: nudibranch
	Units: unitless
	Description: &lt;p&gt;Nudibranch count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719591.rdf
	Name: limpet
	Units: unitless
	Description: &lt;p&gt;Limpet count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719592.rdf
	Name: worm_red
	Units: unitless
	Description: &lt;p&gt;Red worm count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719593.rdf
	Name: worm_green
	Units: unitless
	Description: &lt;p&gt;Green worm count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719594.rdf
	Name: tubeworm
	Units: unitless
	Description: &lt;p&gt;Tubeworm count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719595.rdf
	Name: ragworm
	Units: unitless
	Description: &lt;p&gt;Ragworm count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719596.rdf
	Name: tunicate_orange
	Units: unitless
	Description: &lt;p&gt;Orange tunicate count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719597.rdf
	Name: tunicate_black
	Units: unitless
	Description: &lt;p&gt;Black tunicate count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719598.rdf
	Name: molgula
	Units: unitless
	Description: &lt;p&gt;Molgula count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719599.rdf
	Name: styela
	Units: unitless
	Description: &lt;p&gt;Styela count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719600.rdf
	Name: ecteinascidia_like_tunicate
	Units: unitless
	Description: &lt;p&gt;Ecteinascidia-like tunicate count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719601.rdf
	Name: white_tunicate_clavelina
	Units: unitless
	Description: &lt;p&gt;White tunicate/clavelina count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719602.rdf
	Name: clear_tunicate
	Units: unitless
	Description: &lt;p&gt;Clear tunicate count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719603.rdf
	Name: stalked_tunicate
	Units: unitless
	Description: &lt;p&gt;Stalked tunicate count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719604.rdf
	Name: encrusting_bryozoan
	Units: unitless
	Description: &lt;p&gt;Encrusting bryozoan count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719605.rdf
	Name: bugula_sp
	Units: unitless
	Description: &lt;p&gt;Bugula sp. count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719606.rdf
	Name: B_neritina
	Units: unitless
	Description: &lt;p&gt;B. neritina count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719607.rdf
	Name: total_count
	Units: unitless
	Description: &lt;p&gt;Total count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719608.rdf
	Name: sponge_tot
	Units: unitless
	Description: &lt;p&gt;Total sponge count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719609.rdf
	Name: anemone_tot
	Units: unitless
	Description: &lt;p&gt;Total anemone count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719610.rdf
	Name: hydroid_tot
	Units: unitless
	Description: &lt;p&gt;Total hydroid count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719611.rdf
	Name: barnacle_tot
	Units: unitless
	Description: &lt;p&gt;Total barnacle count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719612.rdf
	Name: oyster_tot
	Units: unitless
	Description: &lt;p&gt;Total oyster count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719613.rdf
	Name: mussel_tot
	Units: unitless
	Description: &lt;p&gt;Total mussel count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719614.rdf
	Name: other_mollusk_tot
	Units: unitless
	Description: &lt;p&gt;Total other mollusk count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719615.rdf
	Name: worm_tot
	Units: unitless
	Description: &lt;p&gt;Total worm count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719616.rdf
	Name: colonial_tun_tot
	Units: unitless
	Description: &lt;p&gt;Total colonial tunicate count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719617.rdf
	Name: solitary_tun_tot
	Units: unitless
	Description: &lt;p&gt;Total solitary tunicate count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719618.rdf
	Name: encr_bry_tot
	Units: unitless
	Description: &lt;p&gt;Total encrusting bryozoan count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719619.rdf
	Name: B_stolon_tot
	Units: unitless
	Description: &lt;p&gt;Total B. stolon count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719620.rdf
	Name: B_neritina_tot
	Units: unitless
	Description: &lt;p&gt;Total B. neritina count (counts summed by taxonomic grouping)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719621.rdf
	Name: total
	Units: unitless
	Description: &lt;p&gt;Total of counts summed by taxonomic grouping&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719622.rdf
	Name: total_minus_bry
	Units: unitless
	Description: &lt;p&gt;Total of counts summed by taxonomic grouping minus the bryozoans&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719623.rdf
	Name: sponge_prop
	Units: unitless
	Description: &lt;p&gt;sponge_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719624.rdf
	Name: anemone_prop
	Units: unitless
	Description: &lt;p&gt;anemone_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719625.rdf
	Name: hydroid_prop
	Units: unitless
	Description: &lt;p&gt;hydroid_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719626.rdf
	Name: barnacle_prop
	Units: unitless
	Description: &lt;p&gt;barnacle_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719627.rdf
	Name: oyster_prop
	Units: unitless
	Description: &lt;p&gt;oyster_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719628.rdf
	Name: mussel_prop
	Units: unitless
	Description: &lt;p&gt;mussel_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719629.rdf
	Name: other_mollusk_prop
	Units: unitless
	Description: &lt;p&gt;other_mullusk_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719630.rdf
	Name: worm_prop
	Units: unitless
	Description: &lt;p&gt;worm_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719631.rdf
	Name: colonial_tun_prop
	Units: unitless
	Description: &lt;p&gt;colonial_tun_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719632.rdf
	Name: solitary_tun_prop
	Units: unitless
	Description: &lt;p&gt;solitary_tun_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719633.rdf
	Name: encr_bry_prop
	Units: unitless
	Description: &lt;p&gt;encr_bry_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719634.rdf
	Name: B_stolon_prop
	Units: unitless
	Description: &lt;p&gt;B_stolon_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719635.rdf
	Name: B_neritina_prop
	Units: unitless
	Description: &lt;p&gt;B_neritina_tot as a proportion&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719636.rdf
	Name: total_prop
	Units: unitless
	Description: &lt;p&gt;Total of proportions&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719637.rdf
	Name: sponge_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Sponge proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719638.rdf
	Name: anemone_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Anemone proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719639.rdf
	Name: hydroid_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Hydroid proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719640.rdf
	Name: barnacle_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Barnacle proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719641.rdf
	Name: mollusks_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Mollusks proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719642.rdf
	Name: worm_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Worm proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719643.rdf
	Name: tunicates_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Tunicates proportion after removing bryozoan counts&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/719644.rdf
	Name: total_minus_bry_prop
	Units: unitless
	Description: &lt;p&gt;Total of proportions after removing bryozoan counts&lt;/p&gt; 
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