http://lod.bco-dmo.org/id/dataset/719916
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-11-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sea star sample information from Ketchikan, AK in February of 2017 (Sea Star Microbiology project)
2017-12-01
publication
2017-12-01
revision
BCO-DMO Linked Data URI
2017-12-01
creation
http://lod.bco-dmo.org/id/dataset/719916
Ian Hewson
Cornell University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hewson, I. (2017) Sea star sample information from Ketchikan, AK in February of 2017 (Sea Star Microbiology project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-12-01 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/719916 [access date]
Sea star sample information Dataset Description: <p>This dataset includes sea star sampling information collected for a survey of sea star-associated densovirus (SSaDV) prevalence and load in asymptomatic animals from near Ketchikan, AK in February of 2017.&nbsp; The data include sample number, species,&nbsp;collection time, depth, latitude, and longitude.&nbsp;</p>
<p>The survey was done to&nbsp;determine the presence/load of SSaDV&nbsp;in SE Alaska during a reported incidence of sea cucumber wasting.</p> Methods and Sampling: <p><strong>Methodology: </strong>This population was surveyed in February 2017 to determine the presence/load of SSaDV in SE Alaska during a&nbsp;reported incidence of sea cucumber wasting.</p>
<p><strong>Sampling and analytical procedures:</strong> Animals were collected by hand at 3 locations near Ketchikan, AK in February 2017. Body wall tissues were biopsied on site to determine the presence/load of SSaDV. Biopsy punches were immediately frozen in liquid N2 before transport back to the lab at Cornell University.</p>
<p>SSaDV abundance/load will be determined by qPCR following the approach of Hewson et al., 2014 (PNAS).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537111 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537111
completed
Ian Hewson
Cornell University
1-607-255-0151
Department of Microbiology Wing Hall 403
Ithaca
NY
14853
USA
hewson@cornell.edu
pointOfContact
asNeeded
Unknown
Sample_num
Date
Time
ISO_DateTime_UTC
Location
Depth
Species
Lat
Long
theme
None, User defined
sample identification
date
time of day
ISO_DateTime_UTC
site
depth
species scientific name (binomial)
latitude
longitude
featureType
BCO-DMO Standard Parameters
SeaStarMicrobiology_Hewson
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbial ecology of sea star wasting disease
http://seastarwastingdisease.wordpress.com
Microbial ecology of sea star wasting disease
<p>Beginning in June 2013 and continuing to present (May 2015), over 20 species of sea stars (Asteroidea, Echinodermata) have been affected by sea star wasting disease (SSWD), affecting populations from central Alaska to Baja California. The disease has lead to greatly reduced abundance or disappearance of these keystone predators, which may result in profound alteration to benthic community structure. Recent work has identified the sea star associated densovirus (SSaDV) as the most likely causative agent of the disease. SSaDV is related to densoviruses inhabiting other echinoderms worldwide, and has been present in West Coast asteroid populations for at least 72 years. Hence, there remain significant knowledge gaps in our understanding of how SSaDV actually elicits SSWD symptoms, especially how the echinoderm host, densovirus and microbiome constituents interact. This project will address three major questions: 1) does viral infection change the composition of the sea star microbiome?, 2) what is the variation of viral genomes and their associated virulence?, and 3) does larval dispersal spread the disease between habitats? This project will address these hypotheses through time-course measurements of host, pathogen and associated microorganisms, genome-genome comparisons between historical and contemporary viral strains, and through experiments targeting larvae and juvenile asteroids in aquaria and in nature. </p>
<p>This project will address three fundamental questions relating to Sea Star Wasting Disease (SSWD): 1) How does SSaDV causes SSWD symptoms and how does the disease progress from primary infection through animal mortality; 2) How do current genotypes of SSaDV vary from those present historically, and is virulence related to genome polymorphisms; and 3) Are larvae and juvenile asteroids differentially affected by SSaDV, and are broadcast-spawned bipinarria a viable mechanism for SSaDV dispersal between distant habitats. The first question will be addressed by experimental inoculation of naïve sea stars with SSaDV, then time-course monitoring of host transcription (i.e. transcriptomics via RNAseq), microbiome composition via 16S rRNA sequencing and quantitative PCR, and viral load and prevalence using quantitatice PCR. The second question will be addressed by amplifying the entire genome of SSaDV and related densoviruses, then perform genome-genome comparisons to identify polymorphic DNA in key protein-encoding regions. The third question will be addressed by collecting bipinarria from plankton at field locations adjacent to spawning asteroid populations, and by performing time-course observations of captive juvenile sea stars and monitoring their bacterial and viral loads using quantitative PCR. This work will be performed primarily in the Salish Sea region, with SSaDV - naïve asteroids collected from Alaskan waters.</p>
Sea Star Microbiology
largerWorkCitation
project
eng; USA
oceans
-131.8306
-131.5677
55.30607
55.44876
2017-02-09
2017-02-12
Salish Sea and Alaskan Waters
0
BCO-DMO catalogue of parameters from Sea star sample information from Ketchikan, AK in February of 2017 (Sea Star Microbiology project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/720254.rdf
Name: Sample_num
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/720255.rdf
Name: Date
Units: unitless
Description: <p>Collection date in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/720256.rdf
Name: Time
Units: unitless
Description: <p>Collection date in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/720257.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Collection timestamp using the ISO 8601:2004(E) standard in format YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/720258.rdf
Name: Location
Units: unitless
Description: <p>Collection location</p>
http://lod.bco-dmo.org/id/dataset-parameter/720259.rdf
Name: Depth
Units: meters
Description: <p>Collection depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/720260.rdf
Name: Species
Units: untiless
Description: <p>Scientific name of collected seastar</p>
http://lod.bco-dmo.org/id/dataset-parameter/720261.rdf
Name: Lat
Units: decimal degrees
Description: <p>Collection latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/720262.rdf
Name: Long
Units: decimal degrees
Description: <p>Collecction longitude</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9931
https://datadocs.bco-dmo.org/file/p99np8MsyL5kQ9/Ketchikan.csv
Ketchikan.csv
Primary data file for dataset ID 719916
download
https://osprey.bco-dmo.org/dataset/719916/data/download
download
onLine
dataset
<p><strong>Methodology: </strong>This population was surveyed in February 2017 to determine the presence/load of SSaDV in SE Alaska during a&nbsp;reported incidence of sea cucumber wasting.</p>
<p><strong>Sampling and analytical procedures:</strong> Animals were collected by hand at 3 locations near Ketchikan, AK in February 2017. Body wall tissues were biopsied on site to determine the presence/load of SSaDV. Biopsy punches were immediately frozen in liquid N2 before transport back to the lab at Cornell University.</p>
<p>SSaDV abundance/load will be determined by qPCR following the approach of Hewson et al., 2014 (PNAS).</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* Species name changed from Pycnopodia helianthodies&nbsp;to accepted name&nbsp;Pycnopodia helianthoides&nbsp; &nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=240764" target="_blank">240764</a>.&nbsp; Based on World Register of Marine Species (WoRMS, accessed 2017-10-17) and communication with the data contributor.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: SeaStarMicrobiology_Hewson
SeaStarMicrobiology_Hewson
shoreside Alaska
shoreside
SeaStarMicrobiology_Hewson
Ian Hewson
shoreside Alaska
shoreside