http://lod.bco-dmo.org/id/dataset/720234
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-12-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
High Resolution sampling of dissolved organic matter (DOM) around Mo'orea coupled with macroalgal collections (Coral DOM2 project)
2017-12-01
publication
2017-12-01
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-28
publication
https://doi.org/10.1575/1912/bco-dmo.720234.1
Craig A. Carlson
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Carlson, C. (2017) High Resolution sampling of dissolved organic matter (DOM) around Mo'orea coupled with macroalgal collections (Coral DOM2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-12-01 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.720234.1 [access date]
High resolution DOM, Moorea, 2016 Dataset Description: Methods and Sampling: <p><strong>Procedural overview:</strong></p>
<p>- All samples were collected via boat, using 1L square Polycarbonate bottles (surface grabs).<br />
- Bottles were gravity filtered (combusted 47mm GF/F) into glass vials.<br />
- Samples were returned to the on-shore MCR LTER laboratory, acidified and stored at Room Temperature.<br />
- Samples were shipped to Craig Carlson’s Laboratory at UCSB for analysis using the HTCO Method (Carlson, et al. 2010 DSRII).</p>
<p><strong>DOC analysis methodology</strong>&nbsp;(from Carlson et al (2010)).</p>
<p>All samples were analyzed via high-temperature combustion on Shimadzu TOC-V analyzers that were slightly modified from the manufacturer’s model system. The condensation coil was removed and the head space of an internal water trap was reduced to minimize system dead space. The combustion tube contained 0.5 cm Pt pillows placed on top of Pt alumina beads to improve peak shape and to reduce alteration of the combustion matrix throughout the analytical run. CO2-free carrier gas was delivered to the TOC-V systems via commercial ultra high purity gas cylinders or a Whatmans gas generator. Three milliliters of sample were drawn into a 5 ml injection syringe, acidified with 2 M HCL (1.5%), and sparged for 1.5 min with CO2-free gas. Three to five replicate 100 ml of sample were injected into the combustion tube heated to 680 1C. A magnesium perchlorate trap was added to the existing water and halide traps to ensure removal of water vapor from the gas line prior to entering a nondispersive infrared detector. The resulting peak area was integrated with Shimadzu chromatographic software.<br />
Extensive conditioning of the combustion tube with repeated injections of low carbon water (LCW) and deep seawater was essential to minimize the machine blanks. The system response was standardized daily with a four-point calibration curve of potassium hydrogen phthalate solution in LCW. Sample and reference swapping and intercalibration exercises were conducted periodically between the UCSB and University of Miami to ensure comparability between sample sets. All samples were systematically referenced against low carbon water, deep Sargasso Sea reference waters (2600 m), and surface Sargasso Sea water every 6-8 analyses (Hansell and Carlson, 1998; Carlson et al., 2004). Daily reference waters were calibrated with DOC Consensus Reference Waters (Hansell, 2005). The standard deviation of the deep and surface references analyzed throughout a run generally had a coefficient of variation ranging between 1-2% over the 3-7 independent analyses (number of references depended on the size of the run), allowing resolution of approximately 1 mmol/kg in the deep waters.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538567 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538567
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538393 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538393
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538428 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538428
completed
Craig A. Carlson
University of California-Santa Barbara
(805) 893-2541
University of California Department of Ecology, Evolution and Marine Biology
Santa Barbara
CA
93106-6150
USA
craig_carlson@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
CRUISE
STATION
TYPE
ISO_DateTime_Local
Latitude
Longitude
Depth
SAMPLE_ID
DOC_FINAL_uM
DOC_SD
FLAG
Shimadzu TOC-V analyzers, modified
theme
None, User defined
Cruise identfier
station
sample type
ISO_DateTime_Local
latitude
longitude
depth
sample identification
dissolved organic Carbon
quality flag
featureType
BCO-DMO Standard Parameters
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
MCR16-1
service
Deployment Activity
Moorea
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts
https://osprey.bco-dmo.org/project/675025
Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts
<p><em>NSF award abstract:</em><br />
Coral reef degradation, whether driven by overfishing, nutrient pollution, declining water quality, or other anthropogenic factors, is associated with a phase shift towards a reefs dominated by fleshy algae. In many cases managing and ameliorating these stressors does not lead to a return to coral dominance, and reefs languish in an algal-dominated state for years. Nearly a decade of research has demonstrated that trajectories toward increasing algal dominance are restructuring microbial community composition and metabolism; the investigators hypothesize that microbial processes facilitate the maintenance of algal dominance by metabolizing organic compounds released by algae thereby stressing corals through hypoxia and disease. The resilience of reefs to these phase shifts is a critical question in coral reef ecology, and managing reefs undergoing these community shifts requires developing an understanding of the role of microbial interactions in facilitating algal overgrowth and altering reef ecosystem function. The research proposed here will investigate the organics produced by algae, the microbes that metabolize the organics, and the impacts of these processes on coral health and growth. This research has implications for managing reef resilience to algal phase shifts by testing the differential resistance of coral-associated microbial communities to algae and defining thresholds of algal species cover which alter ecosystem biogeochemistry. This project provides mentoring across multiple career levels, linking underrepresented undergraduates, two graduate students, a postdoctoral researcher, and a beginning and established investigators.</p>
<p>This project will integrate dissolved organic matter (DOM) geochemistry, microbial genomics and ecosystem process measurements at ecologically-relevant spatial and temporal scales to test hypothetical mechanisms by which microbially-mediated feedbacks may facilitate the spread of fleshy algae on Pacific reef ecosystems. A key product of this research will be understanding how the composition of corals and algae on reefs interact synergistically with complex microbial communities to influence reef ecosystem resilience to algal phase shifts. Emerging molecular and biogeochemical methods will be use to investigate mechanisms of microbial-DOM interactions at multiple spatial and temporal scales. This project will leverage the background environmental data, laboratory facilities and field logistical resources of the Mo'orea Coral Reef Long Term Ecological Research Project in French Polynesia and contribute to the mission of that program of investigating coral reef resilience in the face of global change. The investigators will quantify bulk diel patterns of DOM production and characterize the composition of chromophoric components and both free and acid-hydrolyzable neutral monosaccharides and amino acids from varying benthic algae sources. The team will also characterize planktonic and coral-associated microbial community changes in taxonomic composition and gene expression caused by algal DOM amendments in on-site controlled environmental chambers using phylogenetics and metatranscriptomics, including tracking algal exudate utilization by specific microbial lineages. Field-deployed 100 liter tent mesocosms will be used to examine in situ diel patterns of coupled DOM production and consumption, microbial community genomics and ecosystem metabolism over representative benthic communities comprising combinations of algal and coral species. Together these experimental results will guide interpretation of field surveys of centimeter-scale spatial dynamics of planktonic and coral-associated microbial genomics and metabolism at zones of coral-algal interaction, including boundary layer dynamics of oxygen, bacteria and DOM using planar optodes, high-throughput flow cytometry and fluorescence spectroscopy.</p>
Coral DOM2
largerWorkCitation
project
eng; USA
oceans
Moorea
-149.92
-149.76
-17.6
-17.47
2016-07-26
2016-09-01
Pacific Coral Reefs
0
BCO-DMO catalogue of parameters from High Resolution sampling of dissolved organic matter (DOM) around Mo'orea coupled with macroalgal collections (Coral DOM2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/720243.rdf
Name: CRUISE
Units: unitless
Description: <p>cruise name</p>
http://lod.bco-dmo.org/id/dataset-parameter/720244.rdf
Name: STATION
Units: unitless
Description: <p>MCR site number</p>
http://lod.bco-dmo.org/id/dataset-parameter/720245.rdf
Name: TYPE
Units: unitless
Description: <p>B for bottle - surface bottle grabs</p>
http://lod.bco-dmo.org/id/dataset-parameter/720246.rdf
Name: ISO_DateTime_Local
Units: unitless
Description: <p>Local date and time (UTC-10) in the format yyyy-mm-ddThh:mm:ss</p>
http://lod.bco-dmo.org/id/dataset-parameter/720247.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/720248.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/720249.rdf
Name: Depth
Units: meters
Description: <p>Sampling depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/720250.rdf
Name: SAMPLE_ID
Units: unitless
Description: <p>Unique Identified Code for each sample collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/720251.rdf
Name: DOC_FINAL_uM
Units: Micromol Carbon
Description: <p>Dissolved organic carbon concentration by HTCO. Glass fiber filtrate type GF/F (Whatman). Methodological reference is Carlson et al. 2010 DSRII</p>
http://lod.bco-dmo.org/id/dataset-parameter/720252.rdf
Name: DOC_SD
Units: Micromol Carbon
Description: <p>standard deviation for DOC</p>
http://lod.bco-dmo.org/id/dataset-parameter/720253.rdf
Name: FLAG
Units: unitless
Description: <p>quality flag for DOC [1= acceptable; 4= questionable]</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15482
https://darchive.mblwhoilibrary.org/bitstream/1912/24465/1/dataset-720234_mcrhigh-resdoc__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.720234.1
download
onLine
dataset
<p><strong>Procedural overview:</strong></p>
<p>- All samples were collected via boat, using 1L square Polycarbonate bottles (surface grabs).<br />
- Bottles were gravity filtered (combusted 47mm GF/F) into glass vials.<br />
- Samples were returned to the on-shore MCR LTER laboratory, acidified and stored at Room Temperature.<br />
- Samples were shipped to Craig Carlson’s Laboratory at UCSB for analysis using the HTCO Method (Carlson, et al. 2010 DSRII).</p>
<p><strong>DOC analysis methodology</strong>&nbsp;(from Carlson et al (2010)).</p>
<p>All samples were analyzed via high-temperature combustion on Shimadzu TOC-V analyzers that were slightly modified from the manufacturer’s model system. The condensation coil was removed and the head space of an internal water trap was reduced to minimize system dead space. The combustion tube contained 0.5 cm Pt pillows placed on top of Pt alumina beads to improve peak shape and to reduce alteration of the combustion matrix throughout the analytical run. CO2-free carrier gas was delivered to the TOC-V systems via commercial ultra high purity gas cylinders or a Whatmans gas generator. Three milliliters of sample were drawn into a 5 ml injection syringe, acidified with 2 M HCL (1.5%), and sparged for 1.5 min with CO2-free gas. Three to five replicate 100 ml of sample were injected into the combustion tube heated to 680 1C. A magnesium perchlorate trap was added to the existing water and halide traps to ensure removal of water vapor from the gas line prior to entering a nondispersive infrared detector. The resulting peak area was integrated with Shimadzu chromatographic software.<br />
Extensive conditioning of the combustion tube with repeated injections of low carbon water (LCW) and deep seawater was essential to minimize the machine blanks. The system response was standardized daily with a four-point calibration curve of potassium hydrogen phthalate solution in LCW. Sample and reference swapping and intercalibration exercises were conducted periodically between the UCSB and University of Miami to ensure comparability between sample sets. All samples were systematically referenced against low carbon water, deep Sargasso Sea reference waters (2600 m), and surface Sargasso Sea water every 6-8 analyses (Hansell and Carlson, 1998; Carlson et al., 2004). Daily reference waters were calibrated with DOC Consensus Reference Waters (Hansell, 2005). The standard deviation of the deep and surface references analyzed throughout a run generally had a coefficient of variation ranging between 1-2% over the 3-7 independent analyses (number of references depended on the size of the run), allowing resolution of approximately 1 mmol/kg in the deep waters.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- corrected ISO_DateTime to include reported time rather than 00:00:00; removed separate Time column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Shimadzu TOC-V analyzers, modified
Shimadzu TOC-V analyzers, modified
PI Supplied Instrument Name: Shimadzu TOC-V analyzers, modified Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Deployment: MCR16-1
MCR16-1
Richard B Gump Research Station - Moorea LTER
Richard B Gump Research Station - Moorea LTER
island
MCR16-1
Craig A. Carlson
University of California-Santa Barbara
Richard B Gump Research Station - Moorea LTER
Richard B Gump Research Station - Moorea LTER
island