http://lod.bco-dmo.org/id/dataset/720895
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-12-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater NO3+NO2 and Ammonia from samples collected by pushcore from Guaymas Basin hydrothermal sediments on R/V Atlantis cruise AT37-06 in the Guaymas Basin in December 2016
2017-12-07
publication
2017-12-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.720895.1
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Teske, A. (2020) Porewater NO3+NO2 and Ammonia from samples collected by pushcore from Guaymas Basin hydrothermal sediments on R/V Atlantis cruise AT37-06 in the Guaymas Basin in December 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-12-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.720895.1 [access date]
Porewater NO3+NO2 and Ammonia from samples collected by pushcore from Guaymas Basin hydrothermal sediments on cruise AT37-06 Dataset Description: <p>Porewater NO3+NO2 and Ammonia from samples collected by pushcore from Guaymas Basin hydrothermal sediments on cruise AT37-06 in December 2016.</p> Methods and Sampling: <p>Sampling and Analytical Methodology:&nbsp;Freshly collected sediment cores were sliced on the ship into 3 cm layers; porewater was obtained by gently centrifuging freshly collected sediment in 50 ml conical Falcon tubes for ca. 5 to 10 minutes until the sediment had settled; one Falcon tube produced ca. 8 to 10 ml of porewater.&nbsp; For porewater sulfate measurements, 1 ml subsamples of the overlying porewater were drawn into syringes and injected through 0.45 μm filters into screw cap Eppendorf vials, each acidified with 50 µl of 6N HCl, and then gently bubbled with nitrogen for 4 min to remove sulfide; the samples were then stored at 4ºC before shipping and analysis</p>
<p>Geochemical Analyses:&nbsp;Porewaters from Guaymas Basin sediments were analyzed for Nitrate+Nitrite and ammonia concentrations colorimetrically using a Flow Solutions IV segmented flow Auto Analyzer from O.I Analytical, College Station, TX. The samples were preserved in H2SO4 and were neutralized with NaOH before analysis.&nbsp;Nitrate+Nitrite was determined using the cadmium reduction method and ammonia was determined using the phenate method. Both nutrients were diluted to get their concentrations within the linear range of the auto analyzer. Quality control standards from certified stock standard purchased from Environmental Research Associates, were analyzed every 15-20 samples.&nbsp;&nbsp;</p>
<p>Analysis Notes:<br />
Samples with a NH4 or N+N&nbsp; concentration of greater than 20um were repated at a higher dilution.&nbsp;<br />
Samples were run for NH4 first at a dilution of 40 X (0.1 ml sample to 3.9 ml DDI water).<br />
All NH4 samples were repeated by making a 10x dilution from the 40x dilutions resulting in a 400x dilution.<br />
NH4 and N+N were analyzed separately.<br />
A NaOH solution was made to adjust the pH of the sample to between 5 and 9 upon sample dilution.<br />
The N+N samples were run at a 2x dilution (0.75 ml sample to 0.75 ml NaOH solution).<br />
Repeated 4869-21 (0-3) at 140x dilution; value confirmed.<br />
Repated all remaining&nbsp; N+N samples greater than 20um at 20x dilution.<br />
EPA Method:&nbsp;NO2+NO3 = EPA 353.4; NH4 = EPA 350.1.</p>
<p>Data Processing: The porewater data of Guaymas Basin sand Sonora Margin piston cores were tabulated in Excel sheets.</p>
<p>Quality Control: Note that Sample_IDs "QCS" are internal quality control standards (10 uM) and&nbsp;Sample_IDs "ICV" are external quality control standards.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357238 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357238
completed
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
NO2_NO3
NH4
Flow Solutions IV segmented flow Auto Analyzer
theme
None, User defined
sample identification
Nitrate plus nitrite concentrations in sediment pore water
Ammonium
featureType
BCO-DMO Standard Parameters
Alvin tube core
Nutrient Autoanalyzer
instrument
BCO-DMO Standard Instruments
AT37-06
service
Deployment Activity
Guaymas Basin
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
https://osprey.bco-dmo.org/project/474317
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
<p><em>Description from NSF award abstract:</em><br />
Hydrothermally active sediments in the Guaymas Basin are dominated by novel microbial communities that catalyze important biogeochemical processes in these seafloor ecosystems. This project will investigate genomic potential, physiological capabilities and biogeochemical roles of key uncultured organisms from Guaymas sediments, especially the high-temperature anaerobic methane oxidizers that occur specifically in hydrothermally active sediments (ANME-1Guaymas). The study will focus on their role in carbon transformations, but also explore their potential involvement in sulfur and nitrogen transformations. First-order research topics include quantifying anaerobic methane oxidation under high temperature,in situ concentrations of phosphorus and methane , and with alternate electron acceptors; sulfate and sulfur-dependent microbial pathways and isotopic signatures under these conditions; and nitrogen transformations in methane-oxidizing microbial communities, hydrothermal mats and sediments.</p>
<p>This integrated biogeochemical and microbiological research will explore the pathways of and environmental controls on the consumption and production of methane, other alkanes, inorganic carbon, organic acids and organic matter that fuel the Guaymas sedimentary microbial ecosystem. The hydrothermal sediments of Guaymas Basin provide a spatially compact, high-activity location for investigating novel modes of methane cycling and carbon assimilation into microbial biomass. In the case of anaerobic methane oxidation, the high temperature and pressure tolerance of Guaymas Basin methane-oxidizing microbial communities, and their potential to uncouple from the dominant electron acceptor sulfate, vastly increase the predicted subsurface habitat space and biogeochemical role for anaerobic microbial methanotrophy in global deep subsurface diagenesis. Further, microbial methane production and oxidation interlocks with syulfur and nitrogen transformations, which will be explored at the organism and process level in hydrothermal sediment microbial communities and mats of Guaymas Basin. In general, first-order research tasks (rate measurements, radiotracer incorporation studies, genomes, in situ microgradients) define the key microbial capabilities, pathways and processes that mediate chemical exchange between the subsurface hydrothermal/seeps and deep ocean waters.</p>
Guaymas Basin Interactions
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin
-111
-111
27
27
2016-12-09
2016-12-27
Guaymas Basin, Gulf of California, 27.00 N, 111.00W
0
BCO-DMO catalogue of parameters from Porewater NO3+NO2 and Ammonia from samples collected by pushcore from Guaymas Basin hydrothermal sediments on R/V Atlantis cruise AT37-06 in the Guaymas Basin in December 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/721248.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identification number</p>
http://lod.bco-dmo.org/id/dataset-parameter/721249.rdf
Name: NO2_NO3
Units: micromolar (uM)
Description: <p>NO2+NO3</p>
http://lod.bco-dmo.org/id/dataset-parameter/721250.rdf
Name: NH4
Units: micromolar (uM)
Description: <p>NH4</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1178
https://darchive.mblwhoilibrary.org/bitstream/1912/26108/1/dataset-720895_at37-06-porewater-no3no2-and-ammonia__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.720895.1
download
onLine
dataset
<p>Sampling and Analytical Methodology:&nbsp;Freshly collected sediment cores were sliced on the ship into 3 cm layers; porewater was obtained by gently centrifuging freshly collected sediment in 50 ml conical Falcon tubes for ca. 5 to 10 minutes until the sediment had settled; one Falcon tube produced ca. 8 to 10 ml of porewater.&nbsp; For porewater sulfate measurements, 1 ml subsamples of the overlying porewater were drawn into syringes and injected through 0.45 μm filters into screw cap Eppendorf vials, each acidified with 50 µl of 6N HCl, and then gently bubbled with nitrogen for 4 min to remove sulfide; the samples were then stored at 4ºC before shipping and analysis</p>
<p>Geochemical Analyses:&nbsp;Porewaters from Guaymas Basin sediments were analyzed for Nitrate+Nitrite and ammonia concentrations colorimetrically using a Flow Solutions IV segmented flow Auto Analyzer from O.I Analytical, College Station, TX. The samples were preserved in H2SO4 and were neutralized with NaOH before analysis.&nbsp;Nitrate+Nitrite was determined using the cadmium reduction method and ammonia was determined using the phenate method. Both nutrients were diluted to get their concentrations within the linear range of the auto analyzer. Quality control standards from certified stock standard purchased from Environmental Research Associates, were analyzed every 15-20 samples.&nbsp;&nbsp;</p>
<p>Analysis Notes:<br />
Samples with a NH4 or N+N&nbsp; concentration of greater than 20um were repated at a higher dilution.&nbsp;<br />
Samples were run for NH4 first at a dilution of 40 X (0.1 ml sample to 3.9 ml DDI water).<br />
All NH4 samples were repeated by making a 10x dilution from the 40x dilutions resulting in a 400x dilution.<br />
NH4 and N+N were analyzed separately.<br />
A NaOH solution was made to adjust the pH of the sample to between 5 and 9 upon sample dilution.<br />
The N+N samples were run at a 2x dilution (0.75 ml sample to 0.75 ml NaOH solution).<br />
Repeated 4869-21 (0-3) at 140x dilution; value confirmed.<br />
Repated all remaining&nbsp; N+N samples greater than 20um at 20x dilution.<br />
EPA Method:&nbsp;NO2+NO3 = EPA 353.4; NH4 = EPA 350.1.</p>
<p>Data Processing: The porewater data of Guaymas Basin sand Sonora Margin piston cores were tabulated in Excel sheets.</p>
<p>Quality Control: Note that Sample_IDs "QCS" are internal quality control standards (10 uM) and&nbsp;Sample_IDs "ICV" are external quality control standards.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:&nbsp;<br />
- modified parameter names (replaced spaces with underscores);<br />
- replaced commas in Sample_ID column with semi-colons.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Alvin tube core Instrument Short Name:Tube Core Instrument Description: A plastic tube, about 40 cm (16 inches) long, is pushed into the sediment by Alvin's manipulator arm to collect a sediment core.
Flow Solutions IV segmented flow Auto Analyzer
Flow Solutions IV segmented flow Auto Analyzer
PI Supplied Instrument Name: Flow Solutions IV segmented flow Auto Analyzer PI Supplied Instrument Description:Porewaters from Guayman Basin sediments were analyzed for Nitrate+Nitrite and ammonia concentrations colorimetrically using a Flow Solutions IV segmented flow Auto Analyzer from O.I Analytical, College Station, TX. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: AT37-06
AT37-06
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT37-06
Andreas Teske
University of North Carolina at Chapel Hill
https://datadocs.bco-dmo.org/d3/data_docs/GuaymasBasin_Interactions/AT37-06_CruiseReport.pdf
Report describing AT37-06
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel