<div><p>This data set is associated with PI Douglas Bartlett (NSF OCE-1536776) and R/V Thomas G. Thompson from Apr. 10 - May 20 to the Kermadec Trench adjacent to New Zealand and Schmidt Ocean Institute R/V Falkor cruise FK141109 from Nov. 9 - Dec. 9, 2014, and FK141215 from Dec. 15-21, 2014 to the Mariana Trench. During the cruises, sediment and water samples were collected. Additional details can be found at: <a href="https://schmidtocean.org/cruise/expanding-mariana-trench-perspectives/" target="_blank">https://schmidtocean.org/cruise/expanding-mariana-trench-perspectives/</a> and <a href="https://scripps.ucsd.edu/labs/dbartlett/contact/challenger-deep-cruise-2014/" target="_blank">https://scripps.ucsd.edu/labs/dbartlett/contact/challenger-deep-cruise-2014/</a></p>
<p>Microbes were cultured at 4C on agar plates at 0.1 MPa or in transfer bulbs (Samco, Thermo Fisher Scientific) at either 0.1 MPa or high pressure. Enrichments from the Kermadec Trench were conducted using 2216 Marine Medium (2216; BD DifcoTM), A1 Medium, or a seawater minimal medium, while those from the Mariana Trench were conducted in 2216 only. For incubations at high pressure the media was inoculated, mixed with gelatin at a final concentration of 4%, transferred into bulbs, and incubated at the desired pressure (Yayanos, 2001). Kermadec Trench samples were incubated at 100 MPa while those from the Mariana Trench were incubated at in situ pressure (40-110 MPa). After ~2 months, colony forming units (CFUs) were calculated and representative isolates identified via PCR using the primers 27F and 1492R.</p></div>
Isolates from Mariana and Kermadec trenches, 2014
<div><p>This dataset includes identifications of microbes isolated from hadopelagic seawater and sediment samples from the Mariana and Kermadec trenches from April, November, and December 2014.</p>
<p>NOTE: sample RG10 in this dataset is equivalent to RG09 in the event log, due to at-sea recording error. RG10-2 is equivalent to RG10 in the event log.</p></div>
Isolates
<div><p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reduced Latitude and Longitude precision to 4 decimal places<br />
- replaced commas with semicolons<br />
- added cruise_id, cruise_name, station, date and time deployed and recovered, and local/UTC flag - from ship deployment log datasets</p></div>
721437
Isolates
2017-12-18T16:00:04-05:00
2017-12-18T16:00:04-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:721437
Isolation of microbes from hadal water and sediments from Mariana and Kermadec trenches from R/V Falkor, R/V Thomas G. Thompson FK141109, TN309, FK141215, April to December 2014 (Mariana Perspectives project)
false
Bartlett, D. (2017) Isolation of microbes from hadal water and sediments from Mariana and Kermadec trenches from R/V Falkor, R/V Thomas G. Thompson FK141109, TN309, FK141215, April to December 2014 (Mariana Perspectives project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-12-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/721437 [access date]
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2017-12-18
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