http://lod.bco-dmo.org/id/dataset/722517
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Closed Oyster Mesocosm 15N Tracer (Oyster Reef N2O Emission project)
2017-12-31
publication
2017-12-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-05-08
publication
https://doi.org/10.1575/1912/bco-dmo.722517.1
Craig Tobias
University of Connecticut
principalInvestigator
Mark J. Brush
Virginia Institute of Marine Science
principalInvestigator
Michael F. Piehler
University of North Carolina at Chapel Hill
principalInvestigator
Bongkeun Song
Virginia Institute of Marine Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Tobias, C., Brush, M., Piehler, M., Song, B. (2017) Closed Oyster Mesocosm 15N Tracer (Oyster Reef N2O Emission project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-12-31 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.722517.1 [access date]
N2O, 15N2, 15N Sediment tracer in oyster mesocosms Dataset Description: <p>Closed Oyster Mesocosm 15N Tracer</p> Methods and Sampling: <p><sup>15</sup>N labeled phytoplankton was continuously pumped for three days into 30.5 cm diameter mesocosms&nbsp; containing&nbsp; 10 cm of sediment and 20 liters of overlying water. &nbsp; Three mesocosms contained twelve 7-cm long oysters (O1, O2, O2) and three mesocosms had no oysters and served as controls (C1,C2,C3). Mesoscosms were flow through with a 0.3 day residence time and open to the atmosphere. After phytoplankton feeding period, oysters were removed and mesocosms allowed to flush for 3 days.&nbsp; Tank feed water was then stopped and mesocosms sealed. Time series N<sub>2</sub>O,&nbsp;<sup>15</sup>N<sub>2</sub>&nbsp;, and sediment&nbsp;<sup>15</sup>N were measured during the period when the mesocom was sealed until the dissolved oxygen reached 2 mgL-1.&nbsp; Then the mesocosms were reopened and the feed water restarted. This first incubation ID is CS1.&nbsp; The mesocosms functioned in flow through mode for several days (rest period) and then the incubation / measurement procedure was repeated.&nbsp; This second incubation period ID is CS2.&nbsp; After CS2, the mesocosms were reopened and put into flow through.&nbsp; In total there were 5 closed system incubation periods.&nbsp; The following ‘rest periods’ (hours) were established between CS1-2, 2-3, 3-4, 4-5: 49 hours, 90 hours, 110.5 hours, 138.5 hours.</p>
<p>Water samples for N<sub>2</sub>O analysis were collected with a peristaltic pump through a syringe needle directly into 12 ml exetainer that had been flushed with N<sub>2</sub> and preserved with KOH to a pH above 12. Approximately six ml sample was collected. N<sub>2</sub>O concentrations in the headspace were measured on a GC-ECD.&nbsp; Water samples for 15N2 samples were collected with a peristaltic pump through a syringe needle directly into 30 ml serum bottles that had been flushed with He and preserved with KOH to a pH above 12. &nbsp; Approximately eight ml sample was collected. <sup>15</sup>N<sub>2</sub> was analyzed by GC - Isotope Ratio Mass Spectrometry (IRMS). Sediment <sup>15</sup>N samples were collected from the mesocoms using a 2cm diameter core.&nbsp; The top 3 cm was retained for analysis.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233372 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233372
completed
Craig Tobias
University of Connecticut
860-405-9140
1080 Shennecossett Rd.
Groton
CT
06340
USA
craig.tobias@uconn.edu
pointOfContact
Mark J. Brush
Virginia Institute of Marine Science
804-684-7402
P.O Box 1346
Gloucester Point
VA
23062
USA
brush@vims.edu
pointOfContact
Michael F. Piehler
University of North Carolina at Chapel Hill
252-726-6841 x160
Institute of Marine Science 3431 Arendell St.
Morehead City
North Carolina
28557
U.S.A.
mpiehler@email.unc.edu
pointOfContact
Bongkeun Song
Virginia Institute of Marine Science
804-684-7411
P.O Box 1346
Gloucester Point
VA
23692
USA
songb@vims.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Incubation
Time_Hours
O1_15N2
O1_N2O
O1_O2
O1_Sed_15N
O2_15N2
O2_N2O
O2_O2
O2_Sed_15N
O3_15N2
O3_N2O
O3_O2
O3_Sed_15N
C1_15N2
C1_N2O
C1_O2
C1_Sed_15N
C2_15N2
C2_N2O
C2_O2
C2_Sed_15N
C3_15N2
C3_N2O
C3_O2
C3_Sed_15N
Costech Elemental Analyzer
Agilent 7890B GC with a Poropak Column
Thermo Delta V IRMS
theme
None, User defined
sample description
duration
Nitrogen
Nitrous Oxide
dissolved Oxygen
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Gas Chromatograph
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbial Regulation of Greenhouse Gas N2O Emission from Intertidal Oyster Reefs
https://osprey.bco-dmo.org/project/527289
Microbial Regulation of Greenhouse Gas N2O Emission from Intertidal Oyster Reefs
<p><em>Extracted from the NSF award abstract:</em></p>
<p>Oyster reefs are biogeochemical hot spots and prominent estuarine habitats that provide disproportionate ecological function. Suspension-feeding eastern oysters, Crassostrea virginica, are capable of improving water quality and diminishing eutrophication by filtering nutrients and particles from the water and depositing them in the sediments. Remineralization of these deposits may enhance sedimentary denitrification that facilitates nitrogen removal in tidal estuaries. However, the scientific underpinning of oyster reef function has been challenged in various studies. In addition, recent studies of filter feeding invertebrates reported the production of nitrous oxide (N2O), a greenhouse gas, as an end product of incomplete denitrification by gut microbes. C. virginica could be another source of N2O flux from intertidal habitats. Preliminary work indicated substantial N2O production from individual oysters. The estimated N2O production from high density oyster reefs may exceed the N2O flux measured from some estuaries. With the new discovery of N2O emission and uncertainty regarding eutrophication control, the ecological value of oyster reef restoration may become equivocal.</p>
<p>This project will quantify N2O fluxes to understand the factors controlling N2O emission from oyster reefs. Sedimentary N processes will be examined to develop an oyster reef N model to estimate N2O emission from tidal creek estuaries relative to other N cycling processes. The PIs hypothesize that intertidal oyster reefs are a substantial source of N2O emission from estuarine ecosystems and the magnitude of emission may be linked to water quality. If substantial N2O flux from oyster reefs is validated, ecological benefits of oyster reef restoration should be reevaluated. This interdisciplinary research team includes a microbial ecologist, a biogeochemist, an ecologist and an ecosystem modeler. They will utilize stable isotope and molecular microbiological techniques to quantify oyster N2O production, elucidate microbial sources of N2O emission from oysters and sediments, and estimate seasonal variation of N2O fluxes from oyster reefs. Measurements from this study will be integrated into a coupled oyster bioenergetics-sediment biogeochemistry model to compare system level rates of N cycling on oyster reefs as a function of oyster density and water quality. Modeling results will be used to assess the relative trade-offs of oyster restoration associated with N cycling. They expect to deliver the following end products:1) estimation of annual N2O flux from oyster reefs as an additional source of greenhouse gases from estuaries, 2) a better understanding of the environmental and microbial factors influencing N2O and N2 fluxes in tidal estuaries, 3) transformative knowledge for the effect of oyster restoration on water quality enhancement and ecosystem function, 4) direct guidance for oyster restoration projects whose goals include water quality enhancement, and 5) a modeling tool for use in research and restoration planning.</p>
Oyster Reef N2O Emission
largerWorkCitation
project
eng; USA
oceans
2017-12-31
0
BCO-DMO catalogue of parameters from Closed Oyster Mesocosm 15N Tracer (Oyster Reef N2O Emission project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/722533.rdf
Name: Incubation
Units: unitless
Description: <p>First Closed Incubation or Second Closed Incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/722534.rdf
Name: Time_Hours
Units: hours
Description: <p>Incubation time per incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/722535.rdf
Name: O1_15N2
Units: permil (/mL)
Description: <p>Oyster mecosm 1 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722536.rdf
Name: O1_N2O
Units: nanomole (nM)
Description: <p>Oyster mecosm 1 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722537.rdf
Name: O1_O2
Units: mgL-1
Description: <p>Oyster mecosm 1 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722538.rdf
Name: O1_Sed_15N
Units: permil (/mL)
Description: <p>Oyster mecosm 1 15N enrichment sediment (0-3 cm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/722539.rdf
Name: O2_15N2
Units: permil (/mL)
Description: <p>Oyster mecosm 2 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722540.rdf
Name: O2_N2O
Units: nanomole (nM)
Description: <p>Oyster mecosm 2 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722541.rdf
Name: O2_O2
Units: mgL-1
Description: <p>Oyster mecosm 2 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722542.rdf
Name: O2_Sed_15N
Units: permil (/mL)
Description: <p>Oyster mecosm 2 15N enrichment sediment (0-3 cm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/722543.rdf
Name: O3_15N2
Units: permil (/mL)
Description: <p>Oyster mecosm 3 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722544.rdf
Name: O3_N2O
Units: nanomole (nM)
Description: <p>Oyster mecosm 3 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722545.rdf
Name: O3_O2
Units: mgL-1
Description: <p>Oyster mecosm 3 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722546.rdf
Name: O3_Sed_15N
Units: permil (/mL)
Description: <p>Oyster mecosm 3 15N enrichment sediment (0-3 cm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/722547.rdf
Name: C1_15N2
Units: permil (/mL)
Description: <p>Control mecosm 1 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722548.rdf
Name: C1_N2O
Units: nanomole (nM)
Description: <p>Control mecosm 1 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722549.rdf
Name: C1_O2
Units: mgL-1
Description: <p>Control mecosm 1 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722550.rdf
Name: C1_Sed_15N
Units: permil (/mL)
Description: <p>Control mecosm 1 15N enrichment sediment (0-3 cm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/722551.rdf
Name: C2_15N2
Units: permil (/mL)
Description: <p>Control mecosm 2 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722552.rdf
Name: C2_N2O
Units: nanomole (nM)
Description: <p>Control mecosm 2 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722553.rdf
Name: C2_O2
Units: mgL-1
Description: <p>Control mecosm 2 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722554.rdf
Name: C2_Sed_15N
Units: permil (/mL)
Description: <p>Control mecosm 2 15N enrichment sediment (0-3 cm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/722555.rdf
Name: C3_15N2
Units: permil (/mL)
Description: <p>Control mecosm 3 15N enrichment of dissolved N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/722556.rdf
Name: C3_N2O
Units: nanomole (nM)
Description: <p>Control mecosm 3 Aqueous N2O concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/722557.rdf
Name: C3_O2
Units: mgL-1
Description: <p>Control mecosm 3 dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/722558.rdf
Name: C3_Sed_15N
Units: permil (/mL)
Description: <p>Control mecosm 3 15N enrichment sediment (0-3 cm)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2083
https://darchive.mblwhoilibrary.org/bitstream/1912/24094/1/dataset-722517_closed-mesocosm-15n-tracer__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.722517.1
download
onLine
dataset
<p><sup>15</sup>N labeled phytoplankton was continuously pumped for three days into 30.5 cm diameter mesocosms&nbsp; containing&nbsp; 10 cm of sediment and 20 liters of overlying water. &nbsp; Three mesocosms contained twelve 7-cm long oysters (O1, O2, O2) and three mesocosms had no oysters and served as controls (C1,C2,C3). Mesoscosms were flow through with a 0.3 day residence time and open to the atmosphere. After phytoplankton feeding period, oysters were removed and mesocosms allowed to flush for 3 days.&nbsp; Tank feed water was then stopped and mesocosms sealed. Time series N<sub>2</sub>O,&nbsp;<sup>15</sup>N<sub>2</sub>&nbsp;, and sediment&nbsp;<sup>15</sup>N were measured during the period when the mesocom was sealed until the dissolved oxygen reached 2 mgL-1.&nbsp; Then the mesocosms were reopened and the feed water restarted. This first incubation ID is CS1.&nbsp; The mesocosms functioned in flow through mode for several days (rest period) and then the incubation / measurement procedure was repeated.&nbsp; This second incubation period ID is CS2.&nbsp; After CS2, the mesocosms were reopened and put into flow through.&nbsp; In total there were 5 closed system incubation periods.&nbsp; The following ‘rest periods’ (hours) were established between CS1-2, 2-3, 3-4, 4-5: 49 hours, 90 hours, 110.5 hours, 138.5 hours.</p>
<p>Water samples for N<sub>2</sub>O analysis were collected with a peristaltic pump through a syringe needle directly into 12 ml exetainer that had been flushed with N<sub>2</sub> and preserved with KOH to a pH above 12. Approximately six ml sample was collected. N<sub>2</sub>O concentrations in the headspace were measured on a GC-ECD.&nbsp; Water samples for 15N2 samples were collected with a peristaltic pump through a syringe needle directly into 30 ml serum bottles that had been flushed with He and preserved with KOH to a pH above 12. &nbsp; Approximately eight ml sample was collected. <sup>15</sup>N<sub>2</sub> was analyzed by GC - Isotope Ratio Mass Spectrometry (IRMS). Sediment <sup>15</sup>N samples were collected from the mesocoms using a 2cm diameter core.&nbsp; The top 3 cm was retained for analysis.</p>
Specified by the Principal Investigator(s)
<p>N<sub>2</sub>O concentrations were calculated from N<sub>2</sub>O calibration curve and corrected for N<sub>2</sub>O solubility in the aqueous phase using the Bunsen coefficient. <sup>15</sup>N<sub>2</sub> was normalized to air and air saturated water standards and reported in the delta notation. Sediment <sup>15</sup>N values were normalized to reference materials and reported in the delta notation.</p>
<p><strong>BCO-DMO Processing:</strong></p>
<ul>
<li>added conventional header with dataset name, PI name, version date</li>
<li>modified parameter names to conform with BCO-DMO naming conventions</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech Elemental Analyzer
Costech Elemental Analyzer
PI Supplied Instrument Name: Costech Elemental Analyzer PI Supplied Instrument Description:Sediment 15N Phyto sample was analyzed on the IRMS coupled to a Costech Elemental Analyzer. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Agilent 7890B GC with a Poropak Column
Agilent 7890B GC with a Poropak Column
PI Supplied Instrument Name: Agilent 7890B GC with a Poropak Column PI Supplied Instrument Description:N2O was measured on a Agilent 7890B GC with a Poropak Column. Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Thermo Delta V IRMS
Thermo Delta V IRMS
PI Supplied Instrument Name: Thermo Delta V IRMS PI Supplied Instrument Description:15N2 was measured on a Thermo Delta V IRMS fitted with a Gas Bench II interface following separation from O2 and Ar on a mol sieve 5A column. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/