http://lod.bco-dmo.org/id/dataset/723277
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
2018-01-11
publication
2018-01-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-20
publication
https://doi.org/10.1575/1912/bco-dmo.723277.1
Suzanne Strom
Western Washington University - Shannon Point Marine Center
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Strom, S. (2018) Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-11 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.723277.1 [access date]
Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments. Dataset Description: <p>Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments.</p> Methods and Sampling: <p><strong>Dinoflagellate culturing:</strong>&nbsp;<em>Alexandrium fundyense </em>(strain CCMP 1911) was obtained from the National Center for Marine Algae and Microbiota (NCMA). The strain was originally isolated from Sequim Bay, Washington, USA. <em>Heterocapsa rotundata</em> (strain K-0483), obtained from the Scandanavian Culture Collection of Algae and Protozoa, was isolated from the southern Kattegat Sea near Denmark. Dinoflagellate cultures were maintained in f/2 medium without added silicate at 15°C under a 12L:12D light cycle and transferred every two to three weeks into new media. Growth irradiance for <em>A. fundyense</em> was 53 µmol photons m<sup>-2</sup> s<sup>-1</sup>; growth irradiance for <em>H. rotundata</em> was 12 µmol photons m<sup>-2</sup> s<sup>-1</sup>.</p>
<p><strong>Cell concentration and cell volume:</strong> Cell concentration (cells mL<sup>-1</sup>) and cell volume (µm<sup>3</sup> cell<sup>-1</sup>) estimates for <em>H. rotundata</em> were obtained from live samples measured with a Beckman Coulter Z2 Particle Count and Size Analyzer with Z2 AccuComp software. For <em>A. fundyense</em>, samples were preserved in acid Lugol’s solution (final concentration 2%). Cells were counted in a 1 ml Sedgewick Rafter chamber using light microscopy; cell volume was estimated from length and width of cells (n = 42 or 83 cells per sample) measured with Leica Application Suite X image analysis software and assuming cell shape was approximated by an oblate ellipsoid.</p>
<p><strong>Chlorophyll-a: </strong>Chl-a concentrations were measured by filtering samples through 0.7 µm effective pore size 25 mm glass fiber filters. Pigments were then extracted in 90% acetone for 24 h (dark, -20°C) and fluorescence was measured on a Turner 10-AU fluorometer using the acidification method. Concentrations are reported per cell and per unit cell volume.</p>
<p><strong>Dissolved and particulate dimethylsulfoniopropionate (DMSP):&nbsp;</strong> DMSP samples (4 ml) were gravity-filtered through precombusted 0.7 µm effective pore size 25 mm glass fiber filters so as not to rupture the cells. For measurement of particulate (intracellular) DMSP (DMSPp), filters were placed into sealed 20-ml glass vials containing 3 ml of 5 N NaOH. For measurement of DMSP in the dissolved (extracellular) phase (DMSPd), the first 4.5 mL of each sample’s filtrate were caught in a 5 ml polystyrene culture tube, which was capped and stored at -80°C. Later, DMSPd samples were thawed and sparged with N<sub>2</sub> gas for 1 min to remove any dimethyl sulfide (DMS) already present. Each sparged sample (4 ml) was then dispensed into a 20-ml vial containing 1 ml of 5 N NaOH, and sealed. Upon being sealed, prepared vials for either analysis were allowed to equilibrate for at least 24 h, allowing for the 1:1 conversion of DMSP to gaseous DMS, detectable via gas chromatography. Standards for DMSPp were prepared from pre-diluted DMSP solutions at the same time that samples were filtered and sealed into vials, while DMSPd standards were made at the same time that samples were sparged and sealed into vials. Samples and standards were analyzed using a Shimadzu Gas Chromatograph 14-A equipped with a flame photometric detector and a Supelco packed Chromosil 330 column. The chromatograph was operated isothermally at 90°C with flow rates of hydrogen, air, and helium (carrier gas) at 50, 60, and 150 kPa, respectively. DMSPp samples and standards were measured via direct injection while DMSPd samples and standards were measured with a headspace sweep (He flow rate 40 kPa). DMSPp concentrations are reported per cell and per unit cell volume. DMSPd concentrations are reported per cell and per unit seawater volume.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1434842 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1434842
completed
Suzanne Strom
Western Washington University - Shannon Point Marine Center
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment_ID
Experiment_Date
Dinoflagellate_species
Total_PAR_during_exposure_treatment
Sampling_timepoint
Replicate_cell_counts
Replicate_cell_volume
Replicate_determinations_chlorophyll_a
Replicate_determinations_chlorophyll_a_per_unit_cell_volume
Replicate_determinations_particulate_DMSP_fmolcell1
Replicate_determinations_particulate_DMSP_mmolL_cellvol1
Replicate_determinations_dissolved_DMSP_nmolL1
Replicate_determinations_dissolved_DMSP_fmolcell1
Replicate_determinations_total_DMSP
Beckman Coulter Z2 Particle Count and Size Analyzer
Shimadzu Gas Chromatograph
theme
None, User defined
experiment id
date
species scientific name (binomial)
PAR
No BCO-DMO term
cell_concentration
volume
chlorophyll a
dimethylsulphoniopropionate concentration
featureType
BCO-DMO Standard Parameters
Automated Cell Counter
Gas Chromatograph
instrument
BCO-DMO Standard Instruments
Strom_2014
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Environmental stress and signaling based on reactive oxygen species among planktonic protists
https://osprey.bco-dmo.org/project/614838
Environmental stress and signaling based on reactive oxygen species among planktonic protists
<p><em>Description from NSF proposal:</em><br />
This proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of <em>Phaeodactylum tricornutum</em> light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species.</p>
<p>Our work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.</p>
Protist signaling
largerWorkCitation
project
eng; USA
oceans
2015-07-15
2015-09-10
Salish Sea: 48.5, -122.75
0
BCO-DMO catalogue of parameters from Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/723353.rdf
Name: Experiment_ID
Units: unitless
Description: <p>Experiment ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/723354.rdf
Name: Experiment_Date
Units: unitless
Description: <p>Experiment date; YYYY/MM/DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/723355.rdf
Name: Dinoflagellate_species
Units: unitless
Description: <p>Species of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/723356.rdf
Name: Total_PAR_during_exposure_treatment
Units: mol photons m^-2
Description: <p>Total dose of photosynthetically active radiation received during light exposure treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/723357.rdf
Name: Sampling_timepoint
Units: unitless
Description: <p>Time sample was taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/723358.rdf
Name: Replicate_cell_counts
Units: cells ml-1
Description: <p>Cell concentration in seawater media</p>
http://lod.bco-dmo.org/id/dataset-parameter/723359.rdf
Name: Replicate_cell_volume
Units: um^3
Description: <p>Volume of individual dinoflagellate cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/723360.rdf
Name: Replicate_determinations_chlorophyll_a
Units: pg cell-1
Description: <p>Chlorophyll-a content of cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/723361.rdf
Name: Replicate_determinations_chlorophyll_a_per_unit_cell_volume
Units: mg L-1
Description: <p>Intracellular concentration of chlorophyll-a</p>
http://lod.bco-dmo.org/id/dataset-parameter/723362.rdf
Name: Replicate_determinations_particulate_DMSP_fmolcell1
Units: fmol cell-1
Description: <p>Dimethylsulfoniopropionate content of cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/723363.rdf
Name: Replicate_determinations_particulate_DMSP_mmolL_cellvol1
Units: mmol L cell vol-1
Description: <p>Intracellular concentration of dimethylsulfoniopropionate</p>
http://lod.bco-dmo.org/id/dataset-parameter/723364.rdf
Name: Replicate_determinations_dissolved_DMSP_nmolL1
Units: nmol L-1
Description: <p>Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium</p>
http://lod.bco-dmo.org/id/dataset-parameter/723365.rdf
Name: Replicate_determinations_dissolved_DMSP_fmolcell1
Units: fmol cell-1
Description: <p>Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium</p>
http://lod.bco-dmo.org/id/dataset-parameter/723366.rdf
Name: Replicate_determinations_total_DMSP
Units: umol L-1
Description: <p>Concentration of dissolved plus particulate dimethylsulfoniopropionate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
14396
https://darchive.mblwhoilibrary.org/bitstream/1912/23844/1/dataset-723277_dinoflagellate-culturing__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.723277.1
download
onLine
dataset
<p><strong>Dinoflagellate culturing:</strong>&nbsp;<em>Alexandrium fundyense </em>(strain CCMP 1911) was obtained from the National Center for Marine Algae and Microbiota (NCMA). The strain was originally isolated from Sequim Bay, Washington, USA. <em>Heterocapsa rotundata</em> (strain K-0483), obtained from the Scandanavian Culture Collection of Algae and Protozoa, was isolated from the southern Kattegat Sea near Denmark. Dinoflagellate cultures were maintained in f/2 medium without added silicate at 15°C under a 12L:12D light cycle and transferred every two to three weeks into new media. Growth irradiance for <em>A. fundyense</em> was 53 µmol photons m<sup>-2</sup> s<sup>-1</sup>; growth irradiance for <em>H. rotundata</em> was 12 µmol photons m<sup>-2</sup> s<sup>-1</sup>.</p>
<p><strong>Cell concentration and cell volume:</strong> Cell concentration (cells mL<sup>-1</sup>) and cell volume (µm<sup>3</sup> cell<sup>-1</sup>) estimates for <em>H. rotundata</em> were obtained from live samples measured with a Beckman Coulter Z2 Particle Count and Size Analyzer with Z2 AccuComp software. For <em>A. fundyense</em>, samples were preserved in acid Lugol’s solution (final concentration 2%). Cells were counted in a 1 ml Sedgewick Rafter chamber using light microscopy; cell volume was estimated from length and width of cells (n = 42 or 83 cells per sample) measured with Leica Application Suite X image analysis software and assuming cell shape was approximated by an oblate ellipsoid.</p>
<p><strong>Chlorophyll-a: </strong>Chl-a concentrations were measured by filtering samples through 0.7 µm effective pore size 25 mm glass fiber filters. Pigments were then extracted in 90% acetone for 24 h (dark, -20°C) and fluorescence was measured on a Turner 10-AU fluorometer using the acidification method. Concentrations are reported per cell and per unit cell volume.</p>
<p><strong>Dissolved and particulate dimethylsulfoniopropionate (DMSP):&nbsp;</strong> DMSP samples (4 ml) were gravity-filtered through precombusted 0.7 µm effective pore size 25 mm glass fiber filters so as not to rupture the cells. For measurement of particulate (intracellular) DMSP (DMSPp), filters were placed into sealed 20-ml glass vials containing 3 ml of 5 N NaOH. For measurement of DMSP in the dissolved (extracellular) phase (DMSPd), the first 4.5 mL of each sample’s filtrate were caught in a 5 ml polystyrene culture tube, which was capped and stored at -80°C. Later, DMSPd samples were thawed and sparged with N<sub>2</sub> gas for 1 min to remove any dimethyl sulfide (DMS) already present. Each sparged sample (4 ml) was then dispensed into a 20-ml vial containing 1 ml of 5 N NaOH, and sealed. Upon being sealed, prepared vials for either analysis were allowed to equilibrate for at least 24 h, allowing for the 1:1 conversion of DMSP to gaseous DMS, detectable via gas chromatography. Standards for DMSPp were prepared from pre-diluted DMSP solutions at the same time that samples were filtered and sealed into vials, while DMSPd standards were made at the same time that samples were sparged and sealed into vials. Samples and standards were analyzed using a Shimadzu Gas Chromatograph 14-A equipped with a flame photometric detector and a Supelco packed Chromosil 330 column. The chromatograph was operated isothermally at 90°C with flow rates of hydrogen, air, and helium (carrier gas) at 50, 60, and 150 kPa, respectively. DMSPp samples and standards were measured via direct injection while DMSPd samples and standards were measured with a headspace sweep (He flow rate 40 kPa). DMSPp concentrations are reported per cell and per unit cell volume. DMSPd concentrations are reported per cell and per unit seawater volume.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<div>- reformatted column names to comply with BCO-DMO standards</div>
<div>- replaced all blank cells with nd</div>
<div>- reformatted data into long format instead of wide</div>
<div>- date reformatted to yyyy/mm/dd</div>
<div>- spaces replaced with underscores</div>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Beckman Coulter Z2 Particle Count and Size Analyzer
Beckman Coulter Z2 Particle Count and Size Analyzer
PI Supplied Instrument Name: Beckman Coulter Z2 Particle Count and Size Analyzer PI Supplied Instrument Description:Used to determine cell concentration Instrument Name: Automated Cell Counter Instrument Short Name:ACC Instrument Description: An instrument that determines the numbers, types or viability of cells present in a sample.
Shimadzu Gas Chromatograph
Shimadzu Gas Chromatograph
PI Supplied Instrument Name: Shimadzu Gas Chromatograph PI Supplied Instrument Description:Used to analyze samples and standards Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Deployment: Strom_2014
Strom_2014
lab Strom
laboratory
Strom_2014
Suzanne Strom
Western Washington University - Shannon Point Marine Center
lab Strom
laboratory