http://lod.bco-dmo.org/id/dataset/723422
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Comparative genomics of color morphs in the coral Montastraea cavernosa (NitroFixCorals project)
2018-01-12
publication
2018-01-12
revision
BCO-DMO Linked Data URI
2018-01-12
creation
http://lod.bco-dmo.org/id/dataset/723422
Michael P. Lesser
University of New Hampshire
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lesser, M. P. (2018) Comparative genomics of color morphs in the coral Montastraea cavernosa (NitroFixCorals project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2018-01-12 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/723422 [access date]
Comparative genomics of color morphs in the coral Montastraea cavernosa Dataset Description: <p>Montastraea&nbsp;cavernosa&nbsp;is a common coral in the Caribbean basin found in several color morphs. To investigate the causes for brown and orange morphs we undertook a genomics approach on corals collected at the same time and depth in the Bahamas. The coral&nbsp;holobiont&nbsp;includes the host, symbiotic dinoflagellates (Symbiodinium spp.), and a diverse microbiome. While the coral host showed significant genetic differentiation between color morphs both the composition of the Symbiodinium spp. communities and the prokaryotic communities did not. Both targeted and global gene expression differences in the transcriptome of the host show no difference in fluorescent proteins while the metatranscriptome of the microbiome shows that pigments such as phycoerythrin and orange carotenoid protein of cyanobacterial origin are significantly greater in orange morphs, which is also consistent with the significantly greater number of cyanobacteria quantified by 16S rRNA reads and flow cytometry. The microbiome of orange color morphs expressed significantly more nitrogenase (nifH) transcripts consistent with their known ability to fix nitrogen. Both coral and Symbiodinium spp. transcriptomes from orange morphs had significantly increased expression of genes related to immune response and apoptosis, which may potentially be involved in maintaining and regulating the unique symbiont population in orange morphs.</p> Methods and Sampling: <p>The methodology can be found in the publication associated with these data:</p>
<p>Jarett JK, MacManes MD, Morrow KM, Pankey MS, Lesser MP (2017) Comparative Genomics of Color Morphs In the Coral Montastraea cavernosa. Scientific Reports 7(1): 16039.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1437054 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1437054
completed
Michael P. Lesser
University of New Hampshire
603-862-3442
Department of Molecular, Cellular and Biomedical Sciences
Durham
NH
03824
USA
mpl@unh.edu
pointOfContact
asNeeded
Dataset Version: Final
Unknown
Data_Link
Dataset_Name
Dataset_Description
theme
None, User defined
external_link
dataset_id
brief description
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Nitrogen Fixing Prokaryotes in Corals: Is Nitrogen Fixation a Core Function of the Coral Microbiome?
https://osprey.bco-dmo.org/project/645509
Nitrogen Fixing Prokaryotes in Corals: Is Nitrogen Fixation a Core Function of the Coral Microbiome?
<p><em>Description from NSF award abstract:</em><br />
Coral reefs provide crucial services for people and are becoming increasingly threatened by climate change. But many important questions about coral reefs remain unanswered, such as how their incredibly high biodiversity has developed and is maintained. It is well known that reef-forming corals consist of many different groups of bacteria and algae that live in the tissues of corals, but the function of most of these microbes remain unknown. One of the reported microbial functions is the process called nitrogen fixation. Nitrogen fixation is better known as a very important process on land. For example, alfalfa in agricultural fields has microbes associated with its roots that can capture nitrogen from the air and replace nitrogen lost from soils in water. The nitrogen cycle in the oceans is less well known, especially in tropical waters where nitrogen levels are low, although nitrogen in coral reef systems is known to derive mainly from nitrogen fixation. Recently nitrogen-fixing bacteria have been discovered in the tissues of corals, which begs the overarching question of this project: how important is the contribution of this fixed nitrogen to corals and to the surrounding environment? This research will integrate with an educational program that fosters critical thinking and cooperative learning among participants by involving undergraduate students who will work closely with the scientists. Students from underrepresented backgrounds will be recruited; the undergraduates will become part of an existing undergraduate research opportunities program where they receive mentoring, financial support, and intensive writing support while conducting original research projects of their own. Also a new program will be developed with the university's veterans office to attract and transition veterans into science fields across campus. Finally, outreach activities will be organized at local public high schools, and some public environmental education lectures about the ecology and biology of coral reefs are planned.</p>
<p>Significant gaps in understanding exist about the role of prokaryotes in the physiology, biochemistry and ecology of reef-forming corals. This project will assess how different prokaryotic communities affect the biogeochemistry of nitrogen, specifically nitrogen fixation, within the coral holobiont and how nitrogen fixation contributes to the dissolved organic nitrogen (DON) content of excreted mucus. The introduction of "new nitrogen" though nitrogen fixation and release of DON could have significant impacts on the biogeochemistry of nitrogen in the adjacent benthic communities surrounding corals. High throughput sequencing tools will be used to quantify the taxonomic and functional diversity of these symbioses in corals from the Pacific and Caribbean, as well as the taxonomic diversity of nifH genes in the tissues of corals. Rates of nitrogen fixation on several coral species from Australia, Hawaii and Curacao will be measured, along with the translocation of fixed nitrogen to all compartments of the coral holobiont, and the contribution of "new nitrogen" to the DON of mucus released to the environment determined. The results will increase our understanding of the taxonomic and functional biodiversity of symbiotic prokaryotes in corals. This project will also help guide future studies on the biogeochemical cycling of coral-derived nitrogen on coral reefs.</p>
NitroFixCorals
largerWorkCitation
project
eng; USA
oceans
2018-01-12
Heron Island, Australia; Kaneohe Bay, Hawaii; Curacao
0
BCO-DMO catalogue of parameters from Comparative genomics of color morphs in the coral Montastraea cavernosa (NitroFixCorals project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/723796.rdf
Name: Data_Link
Units: unitless
Description: <p>Link to data. All data is either available to download directly from BCO-DMO, or the link redirects to an external site where the data are available for download.</p>
http://lod.bco-dmo.org/id/dataset-parameter/723797.rdf
Name: Dataset_Name
Units: unitless
Description: <p>Dataset name</p>
http://lod.bco-dmo.org/id/dataset-parameter/723798.rdf
Name: Dataset_Description
Units: unitless
Description: <p>Dataset description</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3223
https://datadocs.bco-dmo.org/file/7DDMm9DIWRDP8q/nitro_fix.csv
nitro_fix.csv
Primary data file for dataset ID 723422
download
https://osprey.bco-dmo.org/dataset/723422/data/download
download
onLine
dataset
<p>The methodology can be found in the publication associated with these data:</p>
<p>Jarett JK, MacManes MD, Morrow KM, Pankey MS, Lesser MP (2017) Comparative Genomics of Color Morphs In the Coral Montastraea cavernosa. Scientific Reports 7(1): 16039.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>The data processing methodology can be found in the publication associated with these data:</p>
<p>Jarett JK, MacManes MD, Morrow KM, Pankey MS, Lesser MP (2017) Comparative Genomics of Color Morphs In the Coral Montastraea&nbsp;cavernosa. Scientific Reports 7(1): 16039.&nbsp;&nbsp;</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- Data from original publication were compiled into one table with dataset descriptions.<br />
- The PI has not verfied&nbsp;any of the information present on the BCO-DMO website.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact