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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/723551.rdf" xlink:actuate="onRequest">Acartia tonsa mortality from Niskin bottles and associated CTD data from R/V Hugh Sharp HRS1316, HRS1317 in mid-Chesapeake Bay, Aug-Sept. 2013 (CopesPopDynHypoZone project)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Roman, M. R., Pierson, J. J. (2018) Acartia tonsa mortality from Niskin bottles and associated CTD data from R/V Hugh Sharp HRS1316, HRS1317 in mid-Chesapeake Bay, Aug-Sept. 2013 (CopesPopDynHypoZone project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2018-01-12 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/723551 [access date]</gco:CharacterString>
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        <gco:CharacterString>Acartia tonsa mortality from Niskin and associated CTD data Dataset Description: &amp;lt;p&amp;gt;This dataset includes&amp;amp;nbsp;&amp;lt;em&amp;gt;Acartia tonsa&amp;lt;/em&amp;gt; mortality from Niskin bottle samples and associated CTD information.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related Datasets:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/707526&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Niskin count and CTD data&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;This is mortality data was obtained from two week-long cruises (1301 in August and 1302 in September) during which Niskin samples were taken from the mid-bay of the Chesapeake from 9 stations in a box formation; 3 stations in a northern transect across the bay (N1-N3), 3 in a midline transect (M1-M3), and 3 in a southern transect (S1-S3).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The Niskin bottles used were General Oceanics 1010x External Spring Water Sampler with a 10L capacity (part number: 101010X). 12 of these were deployed rosette style on the SBE 32 Carousel Water Sampler from the starboard winch of the RV Sharp, along with the SBE 9plus unit which was attached to the rosette. On each downcast, instrument readings were sent from the SBE 9plus unit on the rosette to the SBE 11plus V2 Deck Unit. Based on these data, sampling depths were selected which fell into three areas of interest: above, within, and below the pycnocline.&amp;amp;nbsp; If a pycnocline was not evident and density seemed consistent, only two samples were taken. On the upcast, Niskins were triggered to close electronically from the dry lab.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For mortality assessment, three Niskin bottles were triggered at each chosen depth to give a total sampled volume of 30L. Once on board, Niskin bottles were carefully drained through sieves with 64µm mesh, stained, and preserved following the procedure below, as developed by Elliott and Tang. Sieves with 64µm mesh were selected to catch all life stages of the copepod &amp;lt;em&amp;gt;Acartia tonsa&amp;lt;/em&amp;gt; since &amp;lt;em&amp;gt;Acartia tonsa&amp;lt;/em&amp;gt; eggs are about 75µm in diameter and all subsequent life stages are larger.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sieves were placed in a water bath with the salinity and temperature to which the animals were accustomed. To reduce stress on the copepods, tubes were connected to the Niskin stopcock and squeezed briefly to force air bubbles to flow out; once the water bath began to fill, the flow rate was reduced. Overflow from the water bath was collected in a squirt bottle for later transferring the animals.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Once one Niskin drained, the sieve tube was transferred to the next Niskin to begin draining while the bottom of the drained Niskin was emptied into a wide-mouth plastic beaker, poured into the corresponding sieve, and rinsed with the water collected earlier. After 30L were drained, the contents of each sieve were gently transferred to numbered jars for incubation; date, time, station, depth sampled, and cast numbers were entered into a log.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Neutral red stain was added based on the volume in the jar- the desired concentration was 150 μl per 100ml (for a stock solution of 0.1g Neutral Red powder per 10 ml DI water). The jars were returned to the water baths and incubated in the shade for 15-20 minutes.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After incubation, each sample was vacuum filtered onto a 64μm mesh filter which was transferred to a small petri dish. The dish was capped and labeled with the date, CTD number, and depth of the sample, then wrapped in parafilm to seal. The samples were placed in a Ziploc baggie labeled with the date and given a burst of Flash Freeze, then stored in a -20°C freezer for later analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After returning from the cruises, samples were stored in -20°C freezers; one set (1301) with David Elliott at the Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA, USA and the other (1302) with Jamie Pierson at University of Maryland Center for Environmental Science, Horn Point Laboratory, Cambridge, MD, USA. Samples were individually processed using the procedure developed by Elliott and Tang:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The Petri dish containing the sample was thawed at room temperature, then the mesh was submerged in filtered seawater and gently shaken to resuspend the sample. The sample was then transferred to a counting wheel where it was checked for density and subsampled if necessary.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The sample was then slightly acidified by adding 10% HCl drop-wise until the stained animals distinctly changed from faded pink to bright pink. The sample was then tallied for live or dead status and general stage under dissecting microscope with darkfield illumination. Copepods which were bright red were considered alive at the time of staining; copepods which had no stain, were cloudy white, or were only light pink were considered dead.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To account for individual discrepancies between counters, a calibration count was done during which David Elliott and Catherine Fitzgerald processed the same sample and tallied the live and dead one after the other, with no comparisons until all five samples were completed (a full profile of 3 samples, plus two random samples).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Data were entered into an Excel spreadsheet and checked for transcription errors, then imported into MatLab for data analysis.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/555545.rdf" xlink:title="OCE-1259691" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1259691 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1259691</gmx:Anchor>
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The PIs will develop a mechanistic understanding of how circulation interacts with hypoxia-induced behavioral and physiological changes to affect the population dynamics of coastal zooplankton. They will do this by assessing two potentially contrasting mechanisms influencing the dynamics of the copepod &lt;em&gt;Acartia tonsa&lt;/em&gt; in the hypoxic zone of Chesapeake Bay. The first hypothesis is that maintenance of copepod populations in the hypoxic region requires replenishment by advection (immigration) of animals through wind-driven lateral transport processes. The second, counteractive, hypothesis is that bottom water hypoxia alters the vertical distribution of &lt;em&gt;A. tonsa&lt;/em&gt;, thereby making them more susceptible to advective losses from the region (emigration) via surface water transport in the estuarine circulation. They will take advantage of a current NSF-funded physical oceanography research program in Chesapeake Bay that will comprehensively measure and model axial and lateral water exchanges in the mid-Bay region.&lt;/p&gt;
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	Name: cruise
	Units: unitless
	Description: &lt;p&gt;cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723690.rdf
	Name: date
	Units: unitless
	Description: &lt;p&gt;Gergorian calendar date as recorded in the cruise log formatted as mm/dd/yyyy&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723691.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/723692.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/723693.rdf
	Name: EDT_DOY
	Units: decimal day
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http://lod.bco-dmo.org/id/dataset-parameter/723694.rdf
	Name: station
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/723695.rdf
	Name: CTD_num
	Units: unitless
	Description: &lt;p&gt;Number ID of each CTD/niskin sampling rig cast&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723696.rdf
	Name: Bottle_num
	Units: unitless
	Description: &lt;p&gt;Number ID of niskin bottle in sampling rosette/CTD rig from which the sample was taken&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723697.rdf
	Name: Depth
	Units: meters
	Description: &lt;p&gt;Depth at which sample was taken as recorded in the cruise log&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723698.rdf
	Name: Genus
	Units: unitless
	Description: &lt;p&gt;Genus or least specific identifier of organism in sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723699.rdf
	Name: Species
	Units: unitless
	Description: &lt;p&gt;Species or most specific identifier of organism in sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723700.rdf
	Name: Stage
	Units: unitless
	Description: &lt;p&gt;Life stage of organism in sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723701.rdf
	Name: Alive
	Units: specimens
	Description: &lt;p&gt;Number of organism stained in sample and therefore live&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723702.rdf
	Name: Dead
	Units: specimens
	Description: &lt;p&gt;Number of organism unstained in sample and therefore dead&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723703.rdf
	Name: Percent_live
	Units: unitless
	Description: &lt;p&gt;Number of live organism out of total&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723704.rdf
	Name: Percent_dead
	Units: unitless
	Description: &lt;p&gt;Number of dead organism out of total&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723705.rdf
	Name: Initials_of_counter
	Units: unitless
	Description: &lt;p&gt;Initials of tech or student who counted the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723706.rdf
	Name: Counter_comments_1
	Units: unitless
	Description: &lt;p&gt;Additional comments from the counter about the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723707.rdf
	Name: Counter_comments_2
	Units: unitless
	Description: &lt;p&gt;Additional counter comments&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723708.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Average latitude traveled during collection for each CTD cast&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723709.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Average longitude traveled during collection for each CTD cast&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723710.rdf
	Name: GMT_DOY
	Units: decimal day
	Description: &lt;p&gt;Day of year calculation using CTD recorded GMT time for each cast&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723711.rdf
	Name: Density00
	Units: ?
	Description: &lt;p&gt;Density recorded by CTD sensors for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723712.rdf
	Name: Sigma_t00
	Units: ?
	Description: &lt;p&gt;Density recorded by CTD sensors  for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723713.rdf
	Name: Sal00
	Units: PSU
	Description: &lt;p&gt;Salinity recorded by CTD sensor  for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723714.rdf
	Name: Sal11
	Units: PSU
	Description: &lt;p&gt;Salinity recorded by second CTD sensor  for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723715.rdf
	Name: Sbeox0MgL
	Units: mg/L
	Description: &lt;p&gt;Dissolved oxygen calculated in SEB post-processing from oxygen data recorded by CTD sensors for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723716.rdf
	Name: Sbeox0PS
	Units: unitless
	Description: &lt;p&gt;Dissolved oxygen pressure saturation calculated in SEB post-processing from oxygen data recorded by CTD sensors  for each bottle (percent)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723717.rdf
	Name: OxsatMgL
	Units: unitless
	Description: &lt;p&gt;Dissolved oxygen percent saturation calculated in SEB post-processing from oxygen data recorded by CTD sensors  for each bottle (percent)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723718.rdf
	Name: PrDM
	Units: decibars
	Description: &lt;p&gt;Pressure recorded by CTD sensors  for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723719.rdf
	Name: T090C
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature recorded by CTD sensor for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723720.rdf
	Name: T190C
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature recorded by secondary CTD sensor for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723721.rdf
	Name: FLECO_AFL
	Units: ?
	Description: &lt;p&gt;Fluorescence recorded by CTD sensors for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723722.rdf
	Name: CStarTr0
	Units: unitless
	Description: &lt;p&gt;Transmissometer data recorded for each bottle&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/723723.rdf
	Name: Upoly0
	Units: ?
	Description: &lt;p&gt;Turbidity recorded by CTD sensors for each bottle&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;This is mortality data was obtained from two week-long cruises (1301 in August and 1302 in September) during which Niskin samples were taken from the mid-bay of the Chesapeake from 9 stations in a box formation; 3 stations in a northern transect across the bay (N1-N3), 3 in a midline transect (M1-M3), and 3 in a southern transect (S1-S3).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The Niskin bottles used were General Oceanics 1010x External Spring Water Sampler with a 10L capacity (part number: 101010X). 12 of these were deployed rosette style on the SBE 32 Carousel Water Sampler from the starboard winch of the RV Sharp, along with the SBE 9plus unit which was attached to the rosette. On each downcast, instrument readings were sent from the SBE 9plus unit on the rosette to the SBE 11plus V2 Deck Unit. Based on these data, sampling depths were selected which fell into three areas of interest: above, within, and below the pycnocline.&amp;amp;nbsp; If a pycnocline was not evident and density seemed consistent, only two samples were taken. On the upcast, Niskins were triggered to close electronically from the dry lab.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For mortality assessment, three Niskin bottles were triggered at each chosen depth to give a total sampled volume of 30L. Once on board, Niskin bottles were carefully drained through sieves with 64µm mesh, stained, and preserved following the procedure below, as developed by Elliott and Tang. Sieves with 64µm mesh were selected to catch all life stages of the copepod &amp;lt;em&amp;gt;Acartia tonsa&amp;lt;/em&amp;gt; since &amp;lt;em&amp;gt;Acartia tonsa&amp;lt;/em&amp;gt; eggs are about 75µm in diameter and all subsequent life stages are larger.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sieves were placed in a water bath with the salinity and temperature to which the animals were accustomed. To reduce stress on the copepods, tubes were connected to the Niskin stopcock and squeezed briefly to force air bubbles to flow out; once the water bath began to fill, the flow rate was reduced. Overflow from the water bath was collected in a squirt bottle for later transferring the animals.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Once one Niskin drained, the sieve tube was transferred to the next Niskin to begin draining while the bottom of the drained Niskin was emptied into a wide-mouth plastic beaker, poured into the corresponding sieve, and rinsed with the water collected earlier. After 30L were drained, the contents of each sieve were gently transferred to numbered jars for incubation; date, time, station, depth sampled, and cast numbers were entered into a log.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Neutral red stain was added based on the volume in the jar- the desired concentration was 150 μl per 100ml (for a stock solution of 0.1g Neutral Red powder per 10 ml DI water). The jars were returned to the water baths and incubated in the shade for 15-20 minutes.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After incubation, each sample was vacuum filtered onto a 64μm mesh filter which was transferred to a small petri dish. The dish was capped and labeled with the date, CTD number, and depth of the sample, then wrapped in parafilm to seal. The samples were placed in a Ziploc baggie labeled with the date and given a burst of Flash Freeze, then stored in a -20°C freezer for later analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After returning from the cruises, samples were stored in -20°C freezers; one set (1301) with David Elliott at the Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA, USA and the other (1302) with Jamie Pierson at University of Maryland Center for Environmental Science, Horn Point Laboratory, Cambridge, MD, USA. Samples were individually processed using the procedure developed by Elliott and Tang:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The Petri dish containing the sample was thawed at room temperature, then the mesh was submerged in filtered seawater and gently shaken to resuspend the sample. The sample was then transferred to a counting wheel where it was checked for density and subsampled if necessary.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The sample was then slightly acidified by adding 10% HCl drop-wise until the stained animals distinctly changed from faded pink to bright pink. The sample was then tallied for live or dead status and general stage under dissecting microscope with darkfield illumination. Copepods which were bright red were considered alive at the time of staining; copepods which had no stain, were cloudy white, or were only light pink were considered dead.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To account for individual discrepancies between counters, a calibration count was done during which David Elliott and Catherine Fitzgerald processed the same sample and tallied the live and dead one after the other, with no comparisons until all five samples were completed (a full profile of 3 samples, plus two random samples).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Data were entered into an Excel spreadsheet and checked for transcription errors, then imported into MatLab for data analysis.&amp;lt;/p&amp;gt;</gco:CharacterString>
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Niskin electronic data was post processed using a series of MATLAB scripts to read the raw and processed data, and SBE Data Processing software was used to calculate summary statistics for each bottle.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Live zooplankton samples were stained immediately after capture with Neutral Red vital stain, incubated at ambient collection temperature and salinity for dye uptake, then flash-frozen for later analysis. Samples were sorted under a stereo dissecting microscope within 4 months of collection. General Acartia tonsa stages were identified, and other zooplankton were identified to lowest possible taxonomic level. All zooplankton were classed as live or dead according to staining.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Data Processing Notes:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
- added conventional header with dataset name, PI name, version date&amp;lt;br /&amp;gt;
- modified parameter names to conform with BCO-DMO naming conventions&amp;lt;br /&amp;gt;
- reduced decimal precision&amp;lt;br /&amp;gt;
- replaced commas with semicolons&amp;lt;br /&amp;gt;
- formatted time to 4 digits (added a&amp;amp;nbsp;preceding&amp;amp;nbsp;0 for times less than 1000)&amp;lt;br /&amp;gt;
-&amp;amp;nbsp;moved the long comment columns to the end of the data table&amp;lt;/p&amp;gt;</gco:CharacterString>
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This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/</gco:CharacterString>
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         <gmx:Anchor xlink:href="http://www.ceoe.udel.edu/marine/rvSharp.shtml" xlink:actuate="onRequest">R/V Hugh R. Sharp</gmx:Anchor>
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