http://lod.bco-dmo.org/id/dataset/723966
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013
2018-01-16
publication
2018-01-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.723966.1
Behzad Mortazavi
National Science Foundation
principalInvestigator
William C. Burnett
Florida State University EOAS
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mortazavi, B., Burnett, W. (2018) Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-16 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.723966.1 [access date]
Denitrification and DNRA data. Dataset Description: <p>Denitrification and DNRA data from Little Lagoon, Alabama.</p> Methods and Sampling: <p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>DNRA&nbsp;</strong><br />
Approximately 1 L of outflow water was collected from the inflow water and each core forDNRA analysis. Appropriate sample volume was determined after NH4 + nutrient analysis and expected atom % enrichment. δ15N-NH4 + was measured in samples, constructed blanks, and standards that bracketed the NH4 + concentration of the samples following a modified ammonium diffusion procedure (Holmes et al. 1998) that collects NH4 + dissolved in water by converting NH4 + to NH3 under basic conditions and then traps the NH3 on an acidified glass fiber filter. Non diffused standards were prepared according to Stark and Hart (1996) to account for blank corrections. After 15N analysis on a Europa Scientific SL-2020 system (Stable Isotope Lab, Utah State University), DNRA was calculated from the production rate of 15NH4 + (p15NH4 +) during the incubation according to Christensen et. al (2000): (7) where is the production of 15N-NH4 + and D14 and D15 are the denitrification rates of 14N-NO3 - and 15N-NO3 -, respectively. This assumes that DNRA takes place in the same sediment layers as denitrification and that the 15N labeling of NO3 - being reduced to NH4 + equals the 15N labeling of NO3 - being reduced to N2 (Christensen et al. 2000).</p>
<p><strong>Denitrification and anammox from slurry assays</strong></p>
<p>Volumetric rates of denitrification, anammox, and the relative contribution of anammox to gross N2 production were determined from sediment slurry incubations. Slurry rates for depth-integrated sediments (0-50 mm) were prepared in Exetainers (Thamdrup &amp; Dalsgaard 2002) with artificial seawater (ASW) (70.2g NaCl, 3.0g KCl, 49.4 g MgSO4*7H2O, 5.8g CaCl2*2H2O L-1) constructed at a salinity of 52 and diluted with deionized water to match the salinity of each site. After dilution, homogenized sediment from 0 to 50 mm was added to the ASW and the incubation bottle was sparged with N2 and amended with 100 μmol L-1 Na15NO3 - (99 atom %). Sediment slurry was dispensed to 12 ml Exetainers, yielding approximately 1 ml of sediment and 11 ml ASW with no headspace. For each site, 12 vials total were incubated with three vials stopped at time points 0 to 36 h. Incubations were stopped by adding 250 μL of ZnCl2 and resealing the vials without headspace. Denitrification and anammox rates in slurries were calculated according to equations 5 and 6 described below.&nbsp;</p>
<p>Excess 29N2 and 30N2 concentrations for intact core and slurry incubations were calculated from dissolved 29N2:28N2 and 30N2:28N2 measured using a MIMS. Rates of excess 29N2 (p29) and 30N2 (p30) production were calculated from the flux calculation described above. Rates of ambient 14N2 production (p14) in core incubations with 15NO3 - tracer addition were determined as (Nielsen 1992, Risgaard-Petersen et al. 2003):&nbsp;</p>
<p>(1) p14 = 2 x r14 ± [p29 + p30 ± (1 - r16)]</p>
<p>The 14N:15N ratio of NO3 - undergoing reduction to N2 (r14) was determined as follows:&nbsp;</p>
<p>(2) r14 = [R29 x (1 - ra) - ra] x (2 - ra) ^-1</p>
<p>where R29 was the ratio of p29 to p30 determined for the cores and ra was the relative contribution of anammox to gross N2 production determined in vial slurry incubations. Gross denitrification and anammox rates within intact sediment cores with 15NO3 - tracer addition were calculated as follows:&nbsp;</p>
<p>(3) denitrification = p14 ± (1 - ra)</p>
<p>(4) anammox = p14 ± ra</p>
<p>Denitrification stimulated by the added 15N-NO3 - (D15) was calculated from the classical IPT (Nielsen 1992) and these amended rates are a measure of the denitrification capacity under field conditions when NO3 - is not limiting.&nbsp;</p>
<p>Rates of denitrification and anammox in vial slurry incubations with 15NO3 - amendments were calculated from the equations of Thamdrup and Dalsgaard (2002): (5) (6) where FN was the fraction of 15N in NO3 -. For months when anammox slurry incubations were not performed (August and November 2012), p14 is calculated as D14 from the IPT (Nielsen 1992). Potential denitrification and anammox rates were converted to an areal basis using the wet weight of the sediment in the slurry. All rates and fluxes pertaining to N species in this study were normalized to one atom N.</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0962008 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0962008
completed
Behzad Mortazavi
National Science Foundation
251-861-2141 X2189
University of Alabama, Dauphin Island Sea Laboratory 101 Bienville Blvd
Dauphin Island
AL
36528
bmortazavi@ua.edu
pointOfContact
William C. Burnett
Florida State University EOAS
850-644-6703
Department of Earth, Ocean and Atmospheric Sciences Florida State University
Tallahassee
FL
32206
USA
wburnett@fsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Value_Description
Date
East
East_SE
Mouth
Mouth_SE
West
West_SE
Continuous Flow Analyzer
Europa Scientific SL-2020 system
MIMS
Multichannel proportioning pump
theme
None, User defined
No BCO-DMO term
date
Nitrogen
featureType
BCO-DMO Standard Parameters
Continuous Flow Analyzer
Isotope-ratio Mass Spectrometer
Membrane Inlet Mass Spectrometer
Pump
instrument
BCO-DMO Standard Instruments
LittleLagoon
service
Deployment Activity
Little Lagoon, Alabama
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
https://osprey.bco-dmo.org/project/491318
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
<p> </p>
<p>This project investigated the link between submarine groundwater discharge (SGD) and microalgal dynamics in Little Lagoon, Alabama. In contrast to most near-shore environments, it is fully accessible; has no riverine inputs; and is large enough to display ecological diversity (c. 14x 0.75 km) yet small enough to be comprehensively sampled on appropriate temporal and spatial scales. The PIs have previously demonstrated that the lagoon is a hot-spot for toxic blooms of the diatom <em>Pseudo-nitzchia spp.</em> that are correlated with discharge from the surficial aquifer. This project assessed variability in SGD, the dependence of benthic nutrient fluxes on microphytobenthos (MPB) abundance and productivity, and the response of the phytoplankton to nutrient enrichment and dilution. The work integrated multiple temporal and spatial scales and demonstrated both the relative importance of SGD vs. benthic recycling as a source of nutrients, and the role of SGD in structuring the microalgal community. (<em>paraphrased from Award abstract</em>)</p>
LittleLagoonGroundwater
largerWorkCitation
project
eng; USA
oceans
Little Lagoon, Alabama
-87.773756
-87.773756
30.241929
30.241929
2012-01-01
2013-12-31
southern Alabama, east of Mobile
0
BCO-DMO catalogue of parameters from Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724262.rdf
Name: Year
Units: unitless
Description: <p>Year ID that samples were taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/724263.rdf
Name: Value_Description
Units: unitless
Description: <p>Description of the measurment taken; description includes relevant units for each sample taken; Descriptions include: DIN:DIP = ratio of dissolved inorganic nitrogen to dissolved inorganic phosphate; Denitrification = Denitrification; p14 ambient denitrification = ambient denitrification rates; DNRA = dissimilatory nitrate reduction to ammonium; D15 denitrification = denitrification from added heavy labeled isotope.</p>
http://lod.bco-dmo.org/id/dataset-parameter/724264.rdf
Name: Date
Units: unitless
Description: <p>Month and day that samples were taken; MMM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/724265.rdf
Name: East
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Denitrification and DNRA values collected at the East site; location of site is 30.253347, -87.724729</p>
http://lod.bco-dmo.org/id/dataset-parameter/724266.rdf
Name: East_SE
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Standard error of denitrification and DNRA values collected at the East site</p>
http://lod.bco-dmo.org/id/dataset-parameter/724267.rdf
Name: Mouth
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Denitrification and DNRA values collected at the Mouth site; location of site is 30.243683, -87.738407</p>
http://lod.bco-dmo.org/id/dataset-parameter/724268.rdf
Name: Mouth_SE
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Standard error of denitrification and DNRA values collected at the Mouth site</p>
http://lod.bco-dmo.org/id/dataset-parameter/724269.rdf
Name: West
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Denitrification and DNRA values collected at the West site; location of site is 30.247181, -87.767856</p>
http://lod.bco-dmo.org/id/dataset-parameter/724270.rdf
Name: West_SE
Units: umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
Description: <p>Standard error of denitrification and DNRA values collected at the West site</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2793
https://darchive.mblwhoilibrary.org/bitstream/1912/23830/1/dataset-723966_denitrification-and-dnra__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.723966.1
download
onLine
dataset
<p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>DNRA&nbsp;</strong><br />
Approximately 1 L of outflow water was collected from the inflow water and each core forDNRA analysis. Appropriate sample volume was determined after NH4 + nutrient analysis and expected atom % enrichment. δ15N-NH4 + was measured in samples, constructed blanks, and standards that bracketed the NH4 + concentration of the samples following a modified ammonium diffusion procedure (Holmes et al. 1998) that collects NH4 + dissolved in water by converting NH4 + to NH3 under basic conditions and then traps the NH3 on an acidified glass fiber filter. Non diffused standards were prepared according to Stark and Hart (1996) to account for blank corrections. After 15N analysis on a Europa Scientific SL-2020 system (Stable Isotope Lab, Utah State University), DNRA was calculated from the production rate of 15NH4 + (p15NH4 +) during the incubation according to Christensen et. al (2000): (7) where is the production of 15N-NH4 + and D14 and D15 are the denitrification rates of 14N-NO3 - and 15N-NO3 -, respectively. This assumes that DNRA takes place in the same sediment layers as denitrification and that the 15N labeling of NO3 - being reduced to NH4 + equals the 15N labeling of NO3 - being reduced to N2 (Christensen et al. 2000).</p>
<p><strong>Denitrification and anammox from slurry assays</strong></p>
<p>Volumetric rates of denitrification, anammox, and the relative contribution of anammox to gross N2 production were determined from sediment slurry incubations. Slurry rates for depth-integrated sediments (0-50 mm) were prepared in Exetainers (Thamdrup &amp; Dalsgaard 2002) with artificial seawater (ASW) (70.2g NaCl, 3.0g KCl, 49.4 g MgSO4*7H2O, 5.8g CaCl2*2H2O L-1) constructed at a salinity of 52 and diluted with deionized water to match the salinity of each site. After dilution, homogenized sediment from 0 to 50 mm was added to the ASW and the incubation bottle was sparged with N2 and amended with 100 μmol L-1 Na15NO3 - (99 atom %). Sediment slurry was dispensed to 12 ml Exetainers, yielding approximately 1 ml of sediment and 11 ml ASW with no headspace. For each site, 12 vials total were incubated with three vials stopped at time points 0 to 36 h. Incubations were stopped by adding 250 μL of ZnCl2 and resealing the vials without headspace. Denitrification and anammox rates in slurries were calculated according to equations 5 and 6 described below.&nbsp;</p>
<p>Excess 29N2 and 30N2 concentrations for intact core and slurry incubations were calculated from dissolved 29N2:28N2 and 30N2:28N2 measured using a MIMS. Rates of excess 29N2 (p29) and 30N2 (p30) production were calculated from the flux calculation described above. Rates of ambient 14N2 production (p14) in core incubations with 15NO3 - tracer addition were determined as (Nielsen 1992, Risgaard-Petersen et al. 2003):&nbsp;</p>
<p>(1) p14 = 2 x r14 ± [p29 + p30 ± (1 - r16)]</p>
<p>The 14N:15N ratio of NO3 - undergoing reduction to N2 (r14) was determined as follows:&nbsp;</p>
<p>(2) r14 = [R29 x (1 - ra) - ra] x (2 - ra) ^-1</p>
<p>where R29 was the ratio of p29 to p30 determined for the cores and ra was the relative contribution of anammox to gross N2 production determined in vial slurry incubations. Gross denitrification and anammox rates within intact sediment cores with 15NO3 - tracer addition were calculated as follows:&nbsp;</p>
<p>(3) denitrification = p14 ± (1 - ra)</p>
<p>(4) anammox = p14 ± ra</p>
<p>Denitrification stimulated by the added 15N-NO3 - (D15) was calculated from the classical IPT (Nielsen 1992) and these amended rates are a measure of the denitrification capacity under field conditions when NO3 - is not limiting.&nbsp;</p>
<p>Rates of denitrification and anammox in vial slurry incubations with 15NO3 - amendments were calculated from the equations of Thamdrup and Dalsgaard (2002): (5) (6) where FN was the fraction of 15N in NO3 -. For months when anammox slurry incubations were not performed (August and November 2012), p14 is calculated as D14 from the IPT (Nielsen 1992). Potential denitrification and anammox rates were converted to an areal basis using the wet weight of the sediment in the slurry. All rates and fluxes pertaining to N species in this study were normalized to one atom N.</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data were flagged as below detection limits if no measurable rates were returned after calculations. See equations in methodology section of:</p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
<p><strong>Statistical Analysis</strong></p>
<p>To test the seasonal flux variability between sites in Little Lagoon, two-way ANOVAs with site and date as independent variables were performed. When data could not be transformed to meet ANOVA assumptions, Wilcoxon/Kruskal-Wallis nonparametric tests were used. When significant differences occurred, Tukey HSD or Steel-Dwass post hoc tests were used to determine significant interactions. A Principal component analysis (PCA) was conducted on all biogeochemical parameters to identify underlying multivariate components that may be influencing N fluxes. Spearman’s rho correlation analysis was used to examine the relationship between the principal components and fluxes. Statistical significance of the data set was determined at α=0.05 and error is reported as standard error. All statistical analyses were performed in SAS JMP 10 (SAS Institute Inc.).</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- Data reorganized into one table under one set of column names from both original files<br />
- Units removed from column names<br />
- Column names reformatted to meet BCO-DMO standards<br />
- Information captured in original columns entered under column "Value_Description" where units are also described<br />
- Created column Year to describe to capture the metadata in the file name</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Continuous Flow Analyzer
Continuous Flow Analyzer
PI Supplied Instrument Name: Continuous Flow Analyzer PI Supplied Instrument Description:Used to measure continuous flow rate Instrument Name: Continuous Flow Analyzer Instrument Short Name:CFA Instrument Description: A sample is injected into a flowing carrier solution passing rapidly through small-bore tubing.
Europa Scientific SL-2020 system
Europa Scientific SL-2020 system
PI Supplied Instrument Name: Europa Scientific SL-2020 system PI Supplied Instrument Description:Used for 15N analysis Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
MIMS
MIMS
PI Supplied Instrument Name: MIMS PI Supplied Instrument Description:Used to measure dissolved gas Instrument Name: Membrane Inlet Mass Spectrometer Instrument Short Name:MIMS Instrument Description: Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.
Multichannel proportioning pump
Multichannel proportioning pump
PI Supplied Instrument Name: Multichannel proportioning pump PI Supplied Instrument Description:Used to filter sediment Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Deployment: LittleLagoon
LittleLagoon
SmallBoat_FSU
stationary vessel
LittleLagoon
William C. Burnett
Florida State University EOAS
SmallBoat_FSU
stationary vessel