http://lod.bco-dmo.org/id/dataset/723984
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sediment oxygen demand and ammonium, nitrate plus nitrate, and phosphate flux data from Little Lagoon, Alabama
2018-01-16
publication
2018-01-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.723984.1
Behzad Mortazavi
National Science Foundation
principalInvestigator
William C. Burnett
Florida State University EOAS
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mortazavi, B., Burnett, W. (2018) Sediment oxygen demand and ammonium, nitrate plus nitrate, and phosphate flux data from Little Lagoon, Alabama. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-16 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.723984.1 [access date]
Sediment oxygen demand and ammonium, nitrate plus nitrate, and phosphate flux data. Dataset Description: <p>Sediment oxygen demand and Ammonium, Nitrate plus Nitrate, and Phosphate flux data.</p> Methods and Sampling: <p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>Benthic fluxes from intact sediment cores</strong></p>
<p>At each site triplicate intact sediment cores (270 mm x 95 mm ID; 190 mm sediment, 50 mm water column) were collected and setup in a flow-through system (Lavrentyev et al. 2000) in a darkened environmental chamber set to the average site temperature within 8 hours of collection. The flow-through system consisted of a multichannel proportioning pump that sent 0.7 micron filtered and ~100 μM Na15NO3 - (99 atom %) enriched site water (“inflow”) at a continuous flow rate (1.2 mL min-1) to the overlying water above the sediment surface. The positive displacement of the overlying water exited the core through an outflow tube (“outflow”) and collected in a reservoir. The volume of water overlying each sediment core was exchanged five times during a 24-hour incubation period to equilibrate the cores (Eyre et al. 2002). After the initial 24 hours, triplicate inflow and outflow samples were collected at 36 hours for dissolved gas and nutrient analysis. Dissolved gas analysis followed the modified Isotope pairing technique (IPT) (Nielsen 1992, Risgaard-Petersen et al. 2003). In this approach, rates of 29N2 and 30N2 production from 15NO3 - are quantified and used to calculate rates of 14N2 production (p14) (equation 1, below); when combined with 15N tracer slurry incubations (below), rates of anammox, and the relative contribution of anammox and denitrification to p14 can be determined (equations 2-4, below).</p>
<p>Water samples were collected in 12 mL Exetainers, allowing the vial to overflow two times the tube volume prior to preservation with 250 μL of 50% (w/v) ZnCl2 before being capped. Samples were stored under water in the environmental chamber until dissolved gas analysis on a membrane inlet mass spectrometer (MIMS) equipped with a copper reduction column set at 600oC to remove oxygen (O2) (Kana et al. 1998, Eyre et al. 2002). Benthic nutrient flux samples were filtered (0.7 micron) and immediately frozen until DIN (NO2 -, NO3 -, NH4 +) and PO4 3- analyses as described above.</p>
<p>Sediment oxygen demand (SOD) was measured from oxygen concentrations in inflow and outflow water analyzed with a calibrated microelectrode and a Unisense ® multimeter analyzer. Denitrification and benthic flux calculations (μmol m-2 hr-1) determined flux into or out of the sediment using the influent and effluent concentrations, flow rate (1.2 ml min-1), and the surface area of the sediment (0.00708 m-2). All rates and fluxes pertaining to N species are expressed on a N atom basis. A positive flux indicates release from the sediments to the water column and a negative flux indicates uptake by the sediment.</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0962008 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0962008
completed
Behzad Mortazavi
National Science Foundation
251-861-2141 X2189
University of Alabama, Dauphin Island Sea Laboratory 101 Bienville Blvd
Dauphin Island
AL
36528
bmortazavi@ua.edu
pointOfContact
William C. Burnett
Florida State University EOAS
850-644-6703
Department of Earth, Ocean and Atmospheric Sciences Florida State University
Tallahassee
FL
32206
USA
wburnett@fsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Value_Description
Date
East
East_SE
Mouth
Mouth_SE
West
West_SE
Unisense multimeter analyzer
MIMS
Multichannel proportioning pump
theme
None, User defined
No BCO-DMO term
date
featureType
BCO-DMO Standard Parameters
Gas Analyzer
Membrane Inlet Mass Spectrometer
Pump
instrument
BCO-DMO Standard Instruments
LittleLagoon
service
Deployment Activity
Little Lagoon, Alabama
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
https://osprey.bco-dmo.org/project/491318
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
<p> </p>
<p>This project investigated the link between submarine groundwater discharge (SGD) and microalgal dynamics in Little Lagoon, Alabama. In contrast to most near-shore environments, it is fully accessible; has no riverine inputs; and is large enough to display ecological diversity (c. 14x 0.75 km) yet small enough to be comprehensively sampled on appropriate temporal and spatial scales. The PIs have previously demonstrated that the lagoon is a hot-spot for toxic blooms of the diatom <em>Pseudo-nitzchia spp.</em> that are correlated with discharge from the surficial aquifer. This project assessed variability in SGD, the dependence of benthic nutrient fluxes on microphytobenthos (MPB) abundance and productivity, and the response of the phytoplankton to nutrient enrichment and dilution. The work integrated multiple temporal and spatial scales and demonstrated both the relative importance of SGD vs. benthic recycling as a source of nutrients, and the role of SGD in structuring the microalgal community. (<em>paraphrased from Award abstract</em>)</p>
LittleLagoonGroundwater
largerWorkCitation
project
eng; USA
oceans
Little Lagoon, Alabama
-87.773756
-87.773756
30.241929
30.241929
2010-01-01
2013-12-31
southern Alabama, east of Mobile
0
BCO-DMO catalogue of parameters from Sediment oxygen demand and ammonium, nitrate plus nitrate, and phosphate flux data from Little Lagoon, Alabama
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724253.rdf
Name: Year
Units: unitless
Description: <p>Year ID that samples were taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/724254.rdf
Name: Value_Description
Units: unitless
Description: <p>Description of the measurment taken; description includes relevant units for each sample taken.</p>
http://lod.bco-dmo.org/id/dataset-parameter/724255.rdf
Name: Date
Units: unitless
Description: <p>Month and day that samples were taken; MMM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/724256.rdf
Name: East
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Benthic flux values from the East site; location of site is 30.253347, -87.724729</p>
http://lod.bco-dmo.org/id/dataset-parameter/724257.rdf
Name: East_SE
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Standard error of the benthic flux values from the East site</p>
http://lod.bco-dmo.org/id/dataset-parameter/724258.rdf
Name: Mouth
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Benthic flux values from the Mouth site; location of site is 30.243683, -87.738407</p>
http://lod.bco-dmo.org/id/dataset-parameter/724259.rdf
Name: Mouth_SE
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Standard error of the benthic flux values from the Mouth site</p>
http://lod.bco-dmo.org/id/dataset-parameter/724260.rdf
Name: West
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Benthic flux values from the West site; location of site is 30.247181, -87.767856</p>
http://lod.bco-dmo.org/id/dataset-parameter/724261.rdf
Name: West_SE
Units: umol m-2 hr-1; umol m-2 d-1
Description: <p>Standard error of the benthic flux values from the West site</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5340
https://darchive.mblwhoilibrary.org/bitstream/1912/23808/1/dataset-723984_benthic-flux-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.723984.1
download
onLine
dataset
<p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>Benthic fluxes from intact sediment cores</strong></p>
<p>At each site triplicate intact sediment cores (270 mm x 95 mm ID; 190 mm sediment, 50 mm water column) were collected and setup in a flow-through system (Lavrentyev et al. 2000) in a darkened environmental chamber set to the average site temperature within 8 hours of collection. The flow-through system consisted of a multichannel proportioning pump that sent 0.7 micron filtered and ~100 μM Na15NO3 - (99 atom %) enriched site water (“inflow”) at a continuous flow rate (1.2 mL min-1) to the overlying water above the sediment surface. The positive displacement of the overlying water exited the core through an outflow tube (“outflow”) and collected in a reservoir. The volume of water overlying each sediment core was exchanged five times during a 24-hour incubation period to equilibrate the cores (Eyre et al. 2002). After the initial 24 hours, triplicate inflow and outflow samples were collected at 36 hours for dissolved gas and nutrient analysis. Dissolved gas analysis followed the modified Isotope pairing technique (IPT) (Nielsen 1992, Risgaard-Petersen et al. 2003). In this approach, rates of 29N2 and 30N2 production from 15NO3 - are quantified and used to calculate rates of 14N2 production (p14) (equation 1, below); when combined with 15N tracer slurry incubations (below), rates of anammox, and the relative contribution of anammox and denitrification to p14 can be determined (equations 2-4, below).</p>
<p>Water samples were collected in 12 mL Exetainers, allowing the vial to overflow two times the tube volume prior to preservation with 250 μL of 50% (w/v) ZnCl2 before being capped. Samples were stored under water in the environmental chamber until dissolved gas analysis on a membrane inlet mass spectrometer (MIMS) equipped with a copper reduction column set at 600oC to remove oxygen (O2) (Kana et al. 1998, Eyre et al. 2002). Benthic nutrient flux samples were filtered (0.7 micron) and immediately frozen until DIN (NO2 -, NO3 -, NH4 +) and PO4 3- analyses as described above.</p>
<p>Sediment oxygen demand (SOD) was measured from oxygen concentrations in inflow and outflow water analyzed with a calibrated microelectrode and a Unisense ® multimeter analyzer. Denitrification and benthic flux calculations (μmol m-2 hr-1) determined flux into or out of the sediment using the influent and effluent concentrations, flow rate (1.2 ml min-1), and the surface area of the sediment (0.00708 m-2). All rates and fluxes pertaining to N species are expressed on a N atom basis. A positive flux indicates release from the sediments to the water column and a negative flux indicates uptake by the sediment.</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data were flagged as below detection limits if no measurable rates were returned after calculations. See equations in methodology section of:</p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
<p><strong>Statistical Analysis</strong></p>
<p>To test the seasonal flux variability between sites in Little Lagoon, two-way ANOVAs with site and date as independent variables were performed. When data could not be transformed to meet ANOVA assumptions, Wilcoxon/Kruskal-Wallis nonparametric tests were used. When significant differences occurred, Tukey HSD or Steel-Dwass post hoc tests were used to determine significant interactions. A Principal component analysis (PCA) was conducted on all biogeochemical parameters to identify underlying multivariate components that may be influencing N fluxes. Spearman’s rho correlation analysis was used to examine the relationship between the principal components and fluxes. Statistical significance of the data set was determined at α=0.05 and error is reported as standard error. All statistical analyses were performed in SAS JMP 10 (SAS Institute Inc.).</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- Data reorganized into one table under one set of column names from both original data files<br />
- Units removed from column names<br />
- Column names reformatted to meet BCO-DMO standards<br />
- Information captured in original column names entered under column "Value_Description" where units are also described<br />
- Created column Year to describe to capture the metadata in the file name</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Unisense multimeter analyzer
Unisense multimeter analyzer
PI Supplied Instrument Name: Unisense multimeter analyzer PI Supplied Instrument Description:Used to analyze sediment oxygen demand Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
MIMS
MIMS
PI Supplied Instrument Name: MIMS PI Supplied Instrument Description:Used for dissolved gas analysis Instrument Name: Membrane Inlet Mass Spectrometer Instrument Short Name:MIMS Instrument Description: Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.
Multichannel proportioning pump
Multichannel proportioning pump
PI Supplied Instrument Name: Multichannel proportioning pump PI Supplied Instrument Description:Used to filter sediment cores Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Deployment: LittleLagoon
LittleLagoon
SmallBoat_FSU
stationary vessel
LittleLagoon
William C. Burnett
Florida State University EOAS
SmallBoat_FSU
stationary vessel