http://lod.bco-dmo.org/id/dataset/724158
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Effect of phytosterol supplementation on Acartia egg production (PhytosterolsZooplank project)
2018-01-19
publication
2018-01-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-14
publication
https://doi.org/10.26008/1912/bco-dmo.724158.1
Dr R. Patrick Hassett
Ohio University
principalInvestigator
Dr Jose Giner
State University of New York College of Environmental Science and Forestry
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hassett, R. P., Giner, J. (2021) Effect of phytosterol supplementation on Acartia egg production (PhytosterolsZooplank project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.724158.1 [access date]
Sterols and Acartia egg production Dataset Description: <p>This dataset includes viable egg production of the copepod <em>Acartia </em><em>tonsa</em> that were fed a diet supplemented with a variety of phytosterols at two food concentrations.</p>
<p>Statistical results of this experiment:&nbsp;<a href="https://datadocs.bco-dmo.org/d3/data_docs/PhytosterolsZooplank/Hassett_Sterol_stats2017-12-05.pdf" target="_blank">Hassett_Sterol_stats2017-12-05.pdf</a></p> Methods and Sampling: <p>Phytosterols were synthesized by J. Giner. Rhodomonas cultures were labeled by dissolving sterols in 100% ethanol at a concentration of 2 mg/ml, and then adding the sterol solution at a concentration of 20 µl sterol solution per 100 ml stock culture (at stock density 1x106 cells/liter). The cultures were mixed on a LabGenius orbital shaker for 2 hr at 100 rpm to allow the sterols to bind to the algal surfaces. In vivo chlorophyll a was monitored with a Turner handheld fluorometer to ensure that all food was consumed before the subsequent feeding. This avoided the problem of algal growth diluting the phytosterol concentration over time.</p>
<p><em>Acartia&nbsp;</em><em>tonsa</em> were supplied by AlgaGen Inc, Florida. <em>A. </em><em>tonsa</em> were acclimated for 5 d to a diet of Rhodomonas supplemented with phytosterols. After the acclimation period females were removed and placed in 50 ml Petri dishes. After 24 h females were again removed by pipet, eggs were counted, and the eggs were left for 48h and the number of nauplii counted to determine egg viability. Two experiments were conducted, with the first experiment at low food (≈12,000 cells/ml) and the second at high food (≈25,000 cells/ml). 3 phytosterols were used in both experiments.<br />
&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1061973 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1061973
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1061957 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1061957
completed
Dr R. Patrick Hassett
Ohio University
740-593-2356
Department of Biological Sciences Wilson 202 West Green
Athens
OH
45701
USA
hassett@ohio.edu
pointOfContact
Dr Jose Giner
State University of New York College of Environmental Science and Forestry
315-470-6895
307 Stadium Place
Syracuse
NY
13210
USA
jlginer@syr.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
experiment
sterol_id
sterol_name
replicate
eggs_per_female
viability
Turner hand-held fluorometer
theme
None, User defined
experiment id
treatment
replicate
count
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorometer
In-situ incubator
Microscope - Optical
Shaker
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of Marine Algal Sterols on Zooplankton Growth and Reproduction
https://osprey.bco-dmo.org/project/562648
Collaborative Research: Effects of Marine Algal Sterols on Zooplankton Growth and Reproduction
<p><em>Description from NSF award abstract:</em><br />
Autotroph-herbivore interactions in marine food webs are important to fisheries, the global carbon cycle, and, because of harmful algal blooms, human health. The recent hypothesis that harmful algae interfere with the growth and reproduction of zooplankton because of specific structural modifications of the algal sterols will be tested in research on the roles of nutritional factors in planktonic food webs. The effects of marine algal sterols on herbivorous crustaceans will be investigated in three calanoid copepods,<em> Acartia hudsonica</em>,<em> Eurytemora affinis</em>, and <em>Calanus finmarchicus</em>, and brine shrimp, <em>Artemia salina</em>. In this project, studies will be carried out to determine whether marine algal sterols can be metabolized to cholesterol by zooplankton and the relative efficiency of this process. This information is critical for assessing the nutritional value of different algal diets. Using the metabolic studies as a foundation, further experiments will seek to determine whether selected sterols, some of which have structural similarities to steroid hormones, have an inhibitory impact on the growth and reproduction of crustaceans. The analytical techniques used in these experiments will be high-field 13C-nuclear magnetic resonance spectrometry (NMR) and gas chromatography-high resolution mass spectrometry (GC-HRMS). Test sterols for these experiments will be labeled with stable isotopes (13C and 2H) in specific positions by chemical synthesis.</p>
PhytosterolsZooplank
largerWorkCitation
project
eng; USA
biota
oceans
2018-01-19
0
BCO-DMO catalogue of parameters from Effect of phytosterol supplementation on Acartia egg production (PhytosterolsZooplank project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724172.rdf
Name: experiment
Units: unitless
Description: <p>experiment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/724173.rdf
Name: sterol_id
Units: unitless
Description: <p>sterol identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/724174.rdf
Name: sterol_name
Units: unitless
Description: <p>full chemical name of sterol</p>
http://lod.bco-dmo.org/id/dataset-parameter/724175.rdf
Name: replicate
Units: unitless
Description: <p>replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/724176.rdf
Name: eggs_per_female
Units: eggs
Description: <p>number of eggs produced per female</p>
http://lod.bco-dmo.org/id/dataset-parameter/724177.rdf
Name: viability
Units: unitless
Description: <p>percent of eggs that were viable</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5824
https://darchive.mblwhoilibrary.org/bitstream/1912/26581/1/dataset-724158_sterols-and-acartia-egg-production__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.724158.1
download
onLine
dataset
<p>Phytosterols were synthesized by J. Giner. Rhodomonas cultures were labeled by dissolving sterols in 100% ethanol at a concentration of 2 mg/ml, and then adding the sterol solution at a concentration of 20 µl sterol solution per 100 ml stock culture (at stock density 1x106 cells/liter). The cultures were mixed on a LabGenius orbital shaker for 2 hr at 100 rpm to allow the sterols to bind to the algal surfaces. In vivo chlorophyll a was monitored with a Turner handheld fluorometer to ensure that all food was consumed before the subsequent feeding. This avoided the problem of algal growth diluting the phytosterol concentration over time.</p>
<p><em>Acartia&nbsp;</em><em>tonsa</em> were supplied by AlgaGen Inc, Florida. <em>A. </em><em>tonsa</em> were acclimated for 5 d to a diet of Rhodomonas supplemented with phytosterols. After the acclimation period females were removed and placed in 50 ml Petri dishes. After 24 h females were again removed by pipet, eggs were counted, and the eggs were left for 48h and the number of nauplii counted to determine egg viability. Two experiments were conducted, with the first experiment at low food (≈12,000 cells/ml) and the second at high food (≈25,000 cells/ml). 3 phytosterols were used in both experiments.<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- filled in the empty cells in expt, sterol_id and sterol_name columns with the data from the preceding cell.<br />
- replaced special characters with ascii characters<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner hand-held fluorometer
Turner hand-held fluorometer
PI Supplied Instrument Name: Turner hand-held fluorometer PI Supplied Instrument Description:Used to measure chlorophyll-a. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: PI Supplied Instrument Description:Sanyo MIR252 incubator Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
PI Supplied Instrument Name: PI Supplied Instrument Description:Olympus SZH30 stereo microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
PI Supplied Instrument Name: PI Supplied Instrument Description:LabGenius Digital Orbital Shaker Instrument Name: Shaker Instrument Short Name: Instrument Description: A Shaker is a piece of lab equipment used to mix, blend, or to agitate substances in tube(s) or flask(s) by shaking them, which is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board which is used to place the flasks, beakers, test tubes, etc.