http://lod.bco-dmo.org/id/dataset/724179
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Effect of phytosterol supplementation on Artemia growth (PhytosterolsZooplank project)
2018-01-19
publication
2018-01-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-14
publication
https://doi.org/10.26008/1912/bco-dmo.724179.1
Dr R. Patrick Hassett
Ohio University
principalInvestigator
Dr Jose Giner
State University of New York College of Environmental Science and Forestry
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hassett, R. P., Giner, J. (2021) Effect of phytosterol supplementation on Artemia growth (PhytosterolsZooplank project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.724179.1 [access date]
Sterols and Artemia growth Dataset Description: <p>This dataset includes growth rates measured as biomass&nbsp;change of the brine shrimp <em>Artemia</em> that were fed a diet supplemented with a variety of phytosterols.</p>
<p>Statistical results of this experiment:&nbsp;<a href="https://datadocs.bco-dmo.org/d3/data_docs/PhytosterolsZooplank/Hassett_Sterol_stats2017-12-05.pdf" target="_blank">Hassett_Sterol_stats2017-12-05.pdf</a></p> Methods and Sampling: <p>Phytosterols were synthesized by J. Giner. Rhodomonas cultures were labeled by dissolving sterols in 100% ethanol at a concentration of 2 mg/ml, and then adding the sterol solution at a concentration of 20 µl sterol solution per 100 ml stock culture (at stock density 1x106 cells/liter). The cultures were mixed on a LabGenius orbital shaker for 2 hr at 100 rpm to allow the sterols to bind to the algal surfaces. In vivo chlorophyll a was monitored with a Turner handheld fluorometer to ensure that all food was consumed before the subsequent feeding. This avoided the problem of algal growth diluting the phytosterol concentration over time.</p>
<p>Artemia were hatched in an aerated 2L beaker and the nauplii distributed to 4L beakers containing seawater at 32 ppt. Animals were fed daily with Rhodomonas supplemented with phytosterols at 50,000 cells/ml in 3 replicate, aerated containers for 8 days. In vivo fluorescence was measured with a Turner handheld fluorometer to ensure that the Rhodomonas was consumed between feedings. At the end of the experiment the containers were drained, cleaned of debris, and the number of individuals counted. Samples were then strained on 75µm Nitex and transferred to a preweighed 2.5 cm glass-fiber filter. Filters were dried in a 60 degree C oven overnight and weighed on a Mettler analytical balance. A total of 4 experiments were conducted with Artemia, using 3 replicate treatments of 6 phytosterols, except the last experiment when 5 phytosterols were tested. In the first 3 experiments supplementation was begun 2 days after hatching, while in the fourth experiment supplementation was delayed 2 additional days so that animals began supplementation at a larger size. Results were expressed as both average dry weight per individual and total biomass (mg dry wt) per tank.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1061973 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1061973
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1061957 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1061957
completed
Dr R. Patrick Hassett
Ohio University
740-593-2356
Department of Biological Sciences Wilson 202 West Green
Athens
OH
45701
USA
hassett@ohio.edu
pointOfContact
Dr Jose Giner
State University of New York College of Environmental Science and Forestry
315-470-6895
307 Stadium Place
Syracuse
NY
13210
USA
jlginer@syr.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
experiment
sterol_id
sterol_name
biomass_mg
ind_weight_ug
Turner hand-held fluorometer
Mettler analytical balance
theme
None, User defined
experiment id
treatment
wet_weight_biomass
weight
featureType
BCO-DMO Standard Parameters
Fluorometer
In-situ incubator
Microscope - Optical
scale or balance
Shaker
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of Marine Algal Sterols on Zooplankton Growth and Reproduction
https://osprey.bco-dmo.org/project/562648
Collaborative Research: Effects of Marine Algal Sterols on Zooplankton Growth and Reproduction
<p><em>Description from NSF award abstract:</em><br />
Autotroph-herbivore interactions in marine food webs are important to fisheries, the global carbon cycle, and, because of harmful algal blooms, human health. The recent hypothesis that harmful algae interfere with the growth and reproduction of zooplankton because of specific structural modifications of the algal sterols will be tested in research on the roles of nutritional factors in planktonic food webs. The effects of marine algal sterols on herbivorous crustaceans will be investigated in three calanoid copepods,<em> Acartia hudsonica</em>,<em> Eurytemora affinis</em>, and <em>Calanus finmarchicus</em>, and brine shrimp, <em>Artemia salina</em>. In this project, studies will be carried out to determine whether marine algal sterols can be metabolized to cholesterol by zooplankton and the relative efficiency of this process. This information is critical for assessing the nutritional value of different algal diets. Using the metabolic studies as a foundation, further experiments will seek to determine whether selected sterols, some of which have structural similarities to steroid hormones, have an inhibitory impact on the growth and reproduction of crustaceans. The analytical techniques used in these experiments will be high-field 13C-nuclear magnetic resonance spectrometry (NMR) and gas chromatography-high resolution mass spectrometry (GC-HRMS). Test sterols for these experiments will be labeled with stable isotopes (13C and 2H) in specific positions by chemical synthesis.</p>
PhytosterolsZooplank
largerWorkCitation
project
eng; USA
biota
oceans
2018-01-19
0
BCO-DMO catalogue of parameters from Effect of phytosterol supplementation on Artemia growth (PhytosterolsZooplank project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724192.rdf
Name: experiment
Units: unitless
Description: <p>experiment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/724193.rdf
Name: sterol_id
Units: unitless
Description: <p>sterol identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/724194.rdf
Name: sterol_name
Units: unitless
Description: <p>full chemical name of sterol</p>
http://lod.bco-dmo.org/id/dataset-parameter/724195.rdf
Name: biomass_mg
Units: milligrams
Description: <p>dry weight of Artemia per tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/724196.rdf
Name: ind_weight_ug
Units: micrograms/individual
Description: <p>average individual dry weight</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3212
https://darchive.mblwhoilibrary.org/bitstream/1912/26580/1/dataset-724179_sterols-and-artemia-growth__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.724179.1
download
onLine
dataset
<p>Phytosterols were synthesized by J. Giner. Rhodomonas cultures were labeled by dissolving sterols in 100% ethanol at a concentration of 2 mg/ml, and then adding the sterol solution at a concentration of 20 µl sterol solution per 100 ml stock culture (at stock density 1x106 cells/liter). The cultures were mixed on a LabGenius orbital shaker for 2 hr at 100 rpm to allow the sterols to bind to the algal surfaces. In vivo chlorophyll a was monitored with a Turner handheld fluorometer to ensure that all food was consumed before the subsequent feeding. This avoided the problem of algal growth diluting the phytosterol concentration over time.</p>
<p>Artemia were hatched in an aerated 2L beaker and the nauplii distributed to 4L beakers containing seawater at 32 ppt. Animals were fed daily with Rhodomonas supplemented with phytosterols at 50,000 cells/ml in 3 replicate, aerated containers for 8 days. In vivo fluorescence was measured with a Turner handheld fluorometer to ensure that the Rhodomonas was consumed between feedings. At the end of the experiment the containers were drained, cleaned of debris, and the number of individuals counted. Samples were then strained on 75µm Nitex and transferred to a preweighed 2.5 cm glass-fiber filter. Filters were dried in a 60 degree C oven overnight and weighed on a Mettler analytical balance. A total of 4 experiments were conducted with Artemia, using 3 replicate treatments of 6 phytosterols, except the last experiment when 5 phytosterols were tested. In the first 3 experiments supplementation was begun 2 days after hatching, while in the fourth experiment supplementation was delayed 2 additional days so that animals began supplementation at a larger size. Results were expressed as both average dry weight per individual and total biomass (mg dry wt) per tank.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- filled in the empty cells in expt, sterol_id and sterol_name columns with the data from the preceding cell.<br />
- replaced special characters with ascii characters<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner hand-held fluorometer
Turner hand-held fluorometer
PI Supplied Instrument Name: Turner hand-held fluorometer PI Supplied Instrument Description:Used to measure chlorophyll-a. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: PI Supplied Instrument Description:Sanyo MIR252 incubator Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
PI Supplied Instrument Name: PI Supplied Instrument Description:Olympus SZH30 stereo microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Mettler analytical balance
Mettler analytical balance
PI Supplied Instrument Name: Mettler analytical balance PI Supplied Instrument Description:Used to measure dry weights. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
PI Supplied Instrument Name: PI Supplied Instrument Description:LabGenius Digital Orbital Shaker Instrument Name: Shaker Instrument Short Name: Instrument Description: A Shaker is a piece of lab equipment used to mix, blend, or to agitate substances in tube(s) or flask(s) by shaking them, which is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board which is used to place the flasks, beakers, test tubes, etc.