http://lod.bco-dmo.org/id/dataset/724554
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Lactate in Antarctic krill, Euphausia superba, tissue from a long-term (7-day) trial maintained at ambient conditions, high temperature and/or high CO2 (OA Krill project)
2018-05-03
publication
2018-05-03
revision
BCO-DMO Linked Data URI
2018-05-03
creation
http://lod.bco-dmo.org/id/dataset/724554
Brad Seibel
University of South Florida
principalInvestigator
Grace Saba
Rutgers University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Seibel, B., Saba, G. (2018) Lactate in Antarctic krill, Euphausia superba, tissue from a long-term (7-day) trial maintained at ambient conditions, high temperature and/or high CO2 (OA Krill project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2018-05-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/724554 [access date]
Krill lactate - long-term trial Dataset Description: <p>This dataset includes intracellular (tissue)&nbsp;lactate concentrations for Antarctic krill, <em>Euphausia </em><em>superba,</em>&nbsp;maintained at ambient and high temperature and CO2 levels over a 7-day period.</p> Methods and Sampling: <p><strong>Capture and husbandry:</strong> Antarctic krill (<em>Euphausia </em><em>superba</em>) were captured during austral summers 2013/2014 and 2014/2015. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end)off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to Palmer Research Station. One to two thousand krill were housed in one 4'wx3'h circular holding tank and two 5’x2’x1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton <em>ad libitum</em> throughout the season.</p>
<p><strong>Experimental treatments</strong>: Four experimental treatments were targeted in this study, (1) ambient temperature and ambient CO2, (2) ambient temperature and high CO2 (800ppm), (3) high temperature (3C) and ambient CO2 (4) high temperature&nbsp;and&nbsp;high CO2.Temperature treatments were obtained using two separate recirculating systems. One 800 L cylindrical polycarbonate carboy was attached to a temperature controlled chiller (Delta Star) and inline pump. The carboy was placed in a flow-through water bath and maintained at 0C. A similar system was set up without a chiller and placed in an environmental chamber set at 3C. The systems were replaced with new water and allowed to acclimate to temperature 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then pumped with minimal disturbance into treatment buckets.</p>
<p>Krill of comparable size (average 1 g) were picked and placed in 19 L plastic buckets with airtight lids up to n = 20. Buckets were filled with one of the four treatment waters as described above. Buckets were immediately closed and placed in environmental chambers set to 0C or 3C. Every 24 hours 80% of the water was siphoned out and replaced to minimize excretory and respiratory effect of the animals on treatment conditions. Time points were set at time = 0, 1, 6, 12, 24, 48 hours and 7, 14, 19 days (168, 336, 456 hours). Each bucket was run in duplicate for each treatment at each time point. During season one two 48 hour experiments and three 24 hour long experiments were run. During season two one 24-hour, one 48-hour and one 19-day experiment were completed. Separate buckets were run in duplicate at 0C and 3C for TMAO analysis. For endpoint respirometry, krill were placed one at a time in 300mL airtight glass jars filled with 2um filtered treatment water. Each jar was paired with a blank containing no individuals, placed in water baths, covered, and put in 0C or 3C environmental chambers for the duration of the experiment.</p>
<p><strong>Analyses</strong>: At the end of an experimental duration, blood was immediately taken from 5-10 krill using a 20 gauge needle and pooled. Blood pH was determined using a temperature controlled pH microelectrode (Microelectrodes, Inc. Bedford, NH, USA). Blood lactate was also measured using a handheld lactate meter (Roche Accutrend). The remaining 10 krill were decapitated and their bodies flash frozen for later analysis. Individual tissue (up to n=5) was weighed and homogenized in 500uL homogenate buffer containing 150 mmol L-¹ Potassium fluoride and 5 mmol L-¹ nitrilotriacetic acid (Portner et al., 1990). 75uL homogenate was injected into the Corning 965 total CO2 analyzer, Midland, MI, USA for TCO2 determination. Remaining homogenate was then used to measure pH using the microelectrode as described above. Alternate individuals (up to n=5) were used to measure tissue lactate. Tissue was homogenized 1:1 in DI water using 3 mL glass homogenizers on ice. The supernatant was then measured for lactate using the Accutrend lactate meter. Individuals used for TMAO determination were homogenized 1:5 in 5% trichloroacetic acid then supernatant used with the ferrous sulfate-EDTA method of TMAO determination described by Wekell and Barnett (1991). Endpoint respirometry measurements were taken using a Strathkelvin oxygen electrode and blanks used to correct for residual bacterial respiration.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1641198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1641198
completed
Brad Seibel
University of South Florida
401-744-4927
College of Marine Science 830 1st St SE
St. Petersburg
FL
33701
USA
seibel@usf.edu
pointOfContact
Grace Saba
Rutgers University
848-932-3466
71 Dudley Rd Room 316
New Brunswick
NJ
08901
US
saba@marine.rutgers.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
time_elapsed
treatment_temp
treatment_CO2
Grinding_Buffer_Lactate_mM
O2_consumption
Weight_mg
Volume_Grinding_Buffer_uL
Lactate_mM
comment
Delta Star chiller
Corning 965 total CO2 analyser, Midland, MI, USA
handheld lactate meter (Roche Accutrend)
theme
None, User defined
time_elapsed
treatment
No BCO-DMO term
O2 consumption
weight
volume
comments
featureType
BCO-DMO Standard Parameters
Aquarium chiller
CO2 Analyzer
Homogenizer
Optode
Plankton Net
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
http://coseenow.net/project-parka/
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
<p><em>NSF Award Abstract:</em><br />
Climate change projections for this century suggest that the Southern Ocean will be the first region to be affected by seawater chemistry changes associated with enhanced carbon dioxide (CO2). Additionally, regions of the Southern Ocean are warming faster than any other locations on the planet. Ocean acidification and warming may act synergistically to impair the performance of different organisms by simultaneously increasing metabolic needs and reducing oxygen transport. However, no studies have measured krill acid-base regulation, metabolism, growth, or reproduction in the context of ocean acidification or synergistic ?greenhouse? conditions of elevated CO2 and temperature. In the present project, the investigators will conduct both short and prolonged exposure experiments at Palmer Station, Antarctica to determine the responses of Euphausia superba to elevated CO2 and temperature. The investigators will test hypotheses related to acid-base compensation and acclimation of various life stages of krill to elevated CO2 and temperature. Furthermore, they will determine these impacts on feeding, respiration, metabolism, growth, and reproduction.</p>
<p>The Antarctic krill, Euphausia superba, is a key species in Antarctic food webs as they are a primary food source for many of the top predators in the Southern Ocean including baleen whales, seals, penguins, and other sea birds. This project will determine the responses of Antarctic krill exposed to elevated CO2 and temperature and whether or not krill have the capacity to fully compensate under future ocean conditions. The proposed field effort will be complemented by an extensive broader impact effort focused on bringing marine science to both rural and urban high school students in the Midwest (Kansas). The core educational objectives of this proposal are to 1) instruct students about potential careers in marine science, 2) engage students and promote their interest in the scientific process, critical thinking, and applications of science, mathematics, and technology, and 3) and increase student and teacher awareness and understanding of the oceans and global climate change, with special focus on the Western Antarctic Peninsula region. Finally, this project will engage undergraduate and graduate students in the production, analysis, presentation and publication of datasets.</p>
OA Krill
largerWorkCitation
project
eng; USA
biota
oceans
-64.0533
-64.0533
-64.7743
-64.7743
2018-05-03
Palmer Station, Antarctica
0
BCO-DMO catalogue of parameters from Lactate in Antarctic krill, Euphausia superba, tissue from a long-term (7-day) trial maintained at ambient conditions, high temperature and/or high CO2 (OA Krill project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724630.rdf
Name: time_elapsed
Units: time_elapsed
Description: <p>time since start of experimental trial</p>
http://lod.bco-dmo.org/id/dataset-parameter/724631.rdf
Name: treatment_temp
Units: unitless
Description: <p>temperature treatment; either ambient or high (3C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/724632.rdf
Name: treatment_CO2
Units: unitless
Description: <p>CO2 treatment: either ambient or high (800 ppm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/724633.rdf
Name: Grinding_Buffer_Lactate_mM
Units: milliMolar (mM)
Description: <p>concentration of lactate in the grinding (homogenizting) buffer (grinding buffer is seawater with 20 mM Tris buffer)</p>
http://lod.bco-dmo.org/id/dataset-parameter/724634.rdf
Name: O2_consumption
Units: micromoles Oxygen/gram/hr (umol O2 g-1h-1)
Description: <p>mean oxygen consumption</p>
http://lod.bco-dmo.org/id/dataset-parameter/724635.rdf
Name: Weight_mg
Units: milligrams (mg)
Description: <p>tissue weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/724636.rdf
Name: Volume_Grinding_Buffer_uL
Units: microliters (uL)
Description: <p>volume of grinding buffer (grinding buffer is seawater with 20 mM Tris buffer)</p>
http://lod.bco-dmo.org/id/dataset-parameter/724637.rdf
Name: Lactate_mM
Units: milliMolar (mM)
Description: <p>lactate concentration of tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/724638.rdf
Name: comment
Units: unitless
Description: <p>other comments or notes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1544
https://datadocs.bco-dmo.org/file/R88XmlnIR1q0B4/long_term_trial.csv
long_term_trial.csv
Primary data file for dataset ID 724554
download
https://osprey.bco-dmo.org/dataset/724554/data/download
download
onLine
dataset
<p><strong>Capture and husbandry:</strong> Antarctic krill (<em>Euphausia </em><em>superba</em>) were captured during austral summers 2013/2014 and 2014/2015. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end)off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to Palmer Research Station. One to two thousand krill were housed in one 4'wx3'h circular holding tank and two 5’x2’x1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton <em>ad libitum</em> throughout the season.</p>
<p><strong>Experimental treatments</strong>: Four experimental treatments were targeted in this study, (1) ambient temperature and ambient CO2, (2) ambient temperature and high CO2 (800ppm), (3) high temperature (3C) and ambient CO2 (4) high temperature&nbsp;and&nbsp;high CO2.Temperature treatments were obtained using two separate recirculating systems. One 800 L cylindrical polycarbonate carboy was attached to a temperature controlled chiller (Delta Star) and inline pump. The carboy was placed in a flow-through water bath and maintained at 0C. A similar system was set up without a chiller and placed in an environmental chamber set at 3C. The systems were replaced with new water and allowed to acclimate to temperature 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then pumped with minimal disturbance into treatment buckets.</p>
<p>Krill of comparable size (average 1 g) were picked and placed in 19 L plastic buckets with airtight lids up to n = 20. Buckets were filled with one of the four treatment waters as described above. Buckets were immediately closed and placed in environmental chambers set to 0C or 3C. Every 24 hours 80% of the water was siphoned out and replaced to minimize excretory and respiratory effect of the animals on treatment conditions. Time points were set at time = 0, 1, 6, 12, 24, 48 hours and 7, 14, 19 days (168, 336, 456 hours). Each bucket was run in duplicate for each treatment at each time point. During season one two 48 hour experiments and three 24 hour long experiments were run. During season two one 24-hour, one 48-hour and one 19-day experiment were completed. Separate buckets were run in duplicate at 0C and 3C for TMAO analysis. For endpoint respirometry, krill were placed one at a time in 300mL airtight glass jars filled with 2um filtered treatment water. Each jar was paired with a blank containing no individuals, placed in water baths, covered, and put in 0C or 3C environmental chambers for the duration of the experiment.</p>
<p><strong>Analyses</strong>: At the end of an experimental duration, blood was immediately taken from 5-10 krill using a 20 gauge needle and pooled. Blood pH was determined using a temperature controlled pH microelectrode (Microelectrodes, Inc. Bedford, NH, USA). Blood lactate was also measured using a handheld lactate meter (Roche Accutrend). The remaining 10 krill were decapitated and their bodies flash frozen for later analysis. Individual tissue (up to n=5) was weighed and homogenized in 500uL homogenate buffer containing 150 mmol L-¹ Potassium fluoride and 5 mmol L-¹ nitrilotriacetic acid (Portner et al., 1990). 75uL homogenate was injected into the Corning 965 total CO2 analyzer, Midland, MI, USA for TCO2 determination. Remaining homogenate was then used to measure pH using the microelectrode as described above. Alternate individuals (up to n=5) were used to measure tissue lactate. Tissue was homogenized 1:1 in DI water using 3 mL glass homogenizers on ice. The supernatant was then measured for lactate using the Accutrend lactate meter. Individuals used for TMAO determination were homogenized 1:5 in 5% trichloroacetic acid then supernatant used with the ferrous sulfate-EDTA method of TMAO determination described by Wekell and Barnett (1991). Endpoint respirometry measurements were taken using a Strathkelvin oxygen electrode and blanks used to correct for residual bacterial respiration.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
version 1: 2018-01-23:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- added columns for time_elapsed, treatment_temp, and treatment_CO2 which were combined in a column 'Description'<br />
- replaced spaces with underscores in treatment_temp and treatment_CO2 column</p>
<p>version 2: 2018-05-03:<br />
- added O2_consumption column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Delta Star chiller
Delta Star chiller
PI Supplied Instrument Name: Delta Star chiller PI Supplied Instrument Description:Used to cool water to ambient temperature. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
Corning 965 total CO2 analyser, Midland, MI, USA
Corning 965 total CO2 analyser, Midland, MI, USA
PI Supplied Instrument Name: Corning 965 total CO2 analyser, Midland, MI, USA PI Supplied Instrument Description:Used to measure CO2 in tissues. Instrument Name: CO2 Analyzer Instrument Short Name:CO2 Analyzer Instrument Description: Measures atmospheric carbon dioxide (CO2) concentration. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to homogenize krill tissues. Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.
handheld lactate meter (Roche Accutrend)
handheld lactate meter (Roche Accutrend)
PI Supplied Instrument Name: handheld lactate meter (Roche Accutrend) PI Supplied Instrument Description:Used to measure lactate concentrations in krill blood and tissue. Instrument Name: Optode Instrument Short Name: Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
PI Supplied Instrument Name: PI Supplied Instrument Description:Net with 2 m diameter, 1000 m mesh, non-filtering cod end. Used to collected krill for experimental analyses. Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/