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Water samples were collected from 3 meters depth at the San Pedro Ocean Time-series (SPOT) station (33\u00b033'N, 118\u00b024'W) off the coast of Southern California in March 2015. Six treatments were used: control, nitrate, nitrate+B1, nitrate+B7, nitrate+B12, and nitrate+B1+B7+B12 with triplicate 10L incubations.\u00a0Growth was tracked daily. Samples were collected initially, and at two points during the experiment: exponential growth and stationary phase. Exponential growth occurred at day 7 and stationary growth varied between treatment ranging from 10-12 days. The incubations were co-limited by nitrate and B12. Samples were flash frozen and stored at -80C until analysis. For further details on the methodology, see Suffridge et al (2017).<\/p>\n
Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company).<\/p>\n
Chl-a concentrations were measured using the protocol described by Welschmeyer (1994). 40 ml of water samples from each replicate were filtered through GF\/F glass fiber filters, 3.0-\u03bcm and 8.0-\u03bcm polycarbonate membrane for size fractionated Chl-a analyses. After adding 6 ml of 90% acetone, Chl-a was extracted in the freezer at -20\u00b0C and measured using the non-acidification method with a Turner Designs 10-AUTM fluorometer after 24 hours.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Picoplankton growth with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
This dataset includes chlorophyll-a concentrations and cell counts for picoplankton collected in water samples\u00a0from the San Pedro Ocean Time-series (SPOT), 2015. They were incubated with six treatments of nitrate and vitamin B.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Vitacopss: cell counts and chla","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
BCO-DMO Processing:<\/strong><\/p>\n - added conventional header with dataset name, PI name, version date
\n- renamed parameters to BCO-DMO standard
\n- reformatted columns by adding chla, organism, and cell_count -replacing type, units, and value in order to keep one type of data in each column
\n- changed 'syn' to 'Synechococcus', 'picoeuk' to 'picoeukaryotes'
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