http://lod.bco-dmo.org/id/dataset/725929
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-02-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bacteria, picoeukaryote, and Synecoccus cell counts and chlorophyll-a from nitrate and vitamin-B treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project)
2018-01-26
publication
2018-01-26
revision
BCO-DMO Linked Data URI
2018-01-26
creation
http://lod.bco-dmo.org/id/dataset/725929
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sanudo-Wilhelmy, S. A., Hutchins, D. A., Fu, F. (2018) Bacteria, picoeukaryote, and Synecoccus cell counts and chlorophyll-a from nitrate and vitamin-B treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-26 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/725929 [access date]
Picoplankton growth with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015 Dataset Description: <p>This dataset includes chlorophyll-a concentrations and cell counts for picoplankton collected in water samples&nbsp;from the San Pedro Ocean Time-series (SPOT), 2015. They were incubated with six treatments of nitrate and vitamin B.</p> Methods and Sampling: <p>Water samples were collected from 3 meters depth at the San Pedro Ocean Time-series (SPOT) station (33°33'N, 118°24'W) off the coast of Southern California in March 2015. Six treatments were used: control, nitrate, nitrate+B1, nitrate+B7, nitrate+B12, and nitrate+B1+B7+B12 with triplicate 10L incubations.&nbsp;Growth was tracked daily. Samples were collected initially, and at two points during the experiment: exponential growth and stationary phase. Exponential growth occurred at day 7 and stationary growth varied between treatment ranging from 10-12 days. The incubations were co-limited by nitrate and B12. Samples were flash frozen and stored at -80C until analysis. For further details on the methodology, see Suffridge et al (2017).</p>
<p>Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company).</p>
<p>Chl-a concentrations were measured using the protocol described by Welschmeyer (1994). 40 ml of water samples from each replicate were filtered through GF/F glass fiber filters, 3.0-μm and 8.0-μm polycarbonate membrane for size fractionated Chl-a analyses. After adding 6 ml of 90% acetone, Chl-a was extracted in the freezer at -20°C and measured using the non-acidification method with a Turner Designs 10-AUTM fluorometer after 24 hours.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1435666 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1435666
completed
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
treatment
date
time_point_code
organism
chla
cell_count
BD Accuri C6 flow cytometer (Becton Dickerson and Company)
theme
None, User defined
treatment
date
time_point
taxon
chlorophyll a
count
featureType
BCO-DMO Standard Parameters
CTD - profiler
Flow Cytometer
Fluorometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
lab_Sanudo_2015
service
Deployment Activity
near Los Angeles, California
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
https://osprey.bco-dmo.org/project/516960
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
<p><em>Description from NSF award abstract:</em><br />
B-vitamins (thiamin (B1), biotin (B7), and cobalamin (B12)) are organic molecules used by all organisms for many biochemical reactions ranging from DNA and amino acid synthesis to carbon dioxide assimilation. Despite their metabolic importance, many marine organisms cannot make them and need to obtain them from the environment. Because the requirement for a specific vitamin is different for different organisms, changes in the species composition of algae could be explained by their different B-vitamin requirements. For example, changes in the biological properties of waters during an algal bloom (removal of needed vitamins and release of other vitamins) may favor algae that require the vitamin released by the previous bloom (setting up a floral succession). This selective preconditioning of the waters may be one factor in the seasonal succession of algal species. However, evaluating the role of vitamins in marine ecology has been difficult. No study to date has been comprehensive enough to estimate the importance of vitamins in primary productivity and species succession. This is especially true in coastal upwelling regions that although relatively small in area, are orders of magnitude more productive than their open-ocean counterparts. In fact, those regions contribute a significant portion of the world fisheries. Therefore, in order to try to predict future changes in the world ocean due to human activity, the variables that influence or control the algal communities that dominate the very productive food chains of upwelling regions need to be identified.</p>
<p>This study will investigate how the availability of B-vitamins affects the dynamics of algal- and bacterioplankton population growth in coastal waters of an upwelling region off Southern California. This comprehensive field investigation will determine in situ temporal concentrations of several dissolved and particulate B-vitamins, inorganic micro- and macronutrients, concurrently with seasonal changes in phytoplankton and bacterial abundances and species composition at a long-term time series station within the San Pedro Basin near Los Angeles. Those measurements will be complemented with field incubation experiments with natural plankton assemblages to study the effect of organic and inorganic nutrient amendments on phytoplankton and bacterial community structure. This study will establish for the first time that the availability of ambient B-vitamins influence algal and bacterial species succession in a highly productive coastal upwelling region and that multiple and differing B-vitamin requirements limit growth of some phytoplankton species in those areas. Furthermore, this study will try to show that coastal upwelling transports some B-vitamins to the phytoplankton community in the photic zone from bacterially-influenced source waters within the upper mesopelagic zone.</p>
B-vitamin plankton succession
largerWorkCitation
project
eng; USA
biota
oceans
near Los Angeles, California
-118.3983
-118.3983
33.5478
33.5478
2015-03-12
2015-03-24
Southern California Bight
0
BCO-DMO catalogue of parameters from Bacteria, picoeukaryote, and Synecoccus cell counts and chlorophyll-a from nitrate and vitamin-B treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/726019.rdf
Name: treatment
Units: unitless
Description: <p>treatments: control; nitrate (N); nitrate+B1 (N+B1); nitrate+B7 (N+B7); nitrate+B12 (N+B12); and nitrate+B1+B7+B12 (All)</p>
http://lod.bco-dmo.org/id/dataset-parameter/726020.rdf
Name: date
Units: unitless
Description: <p>date of experiment formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/726021.rdf
Name: time_point_code
Units: unitless
Description: <p>code for sampling period during one of three growth phases of the population: 0=initial sampling; 1=exponential phase; 2=stationary phase. Dates: 2015-03-12 (time 0); 2015-03-20 (time 1); 2015-03-25 (time 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/726022.rdf
Name: organism
Units: unitless
Description: <p>type of microorganism counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/726023.rdf
Name: chla
Units: unitless
Description: <p>chl-a Rf value</p>
http://lod.bco-dmo.org/id/dataset-parameter/726024.rdf
Name: cell_count
Units: cells/milliliter
Description: <p>cell count of microorganism</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
20854
https://datadocs.bco-dmo.org/file/DwwxDqlc5ryZV7/Vitacopss_cell_chla_conc.csv
Vitacopss_cell_chla_conc.csv
Primary data file for dataset ID 725929
download
https://osprey.bco-dmo.org/dataset/725929/data/download
download
onLine
dataset
<p>Water samples were collected from 3 meters depth at the San Pedro Ocean Time-series (SPOT) station (33°33'N, 118°24'W) off the coast of Southern California in March 2015. Six treatments were used: control, nitrate, nitrate+B1, nitrate+B7, nitrate+B12, and nitrate+B1+B7+B12 with triplicate 10L incubations.&nbsp;Growth was tracked daily. Samples were collected initially, and at two points during the experiment: exponential growth and stationary phase. Exponential growth occurred at day 7 and stationary growth varied between treatment ranging from 10-12 days. The incubations were co-limited by nitrate and B12. Samples were flash frozen and stored at -80C until analysis. For further details on the methodology, see Suffridge et al (2017).</p>
<p>Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company).</p>
<p>Chl-a concentrations were measured using the protocol described by Welschmeyer (1994). 40 ml of water samples from each replicate were filtered through GF/F glass fiber filters, 3.0-μm and 8.0-μm polycarbonate membrane for size fractionated Chl-a analyses. After adding 6 ml of 90% acetone, Chl-a was extracted in the freezer at -20°C and measured using the non-acidification method with a Turner Designs 10-AUTM fluorometer after 24 hours.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- reformatted columns by adding chla, organism, and cell_count -replacing type, units, and value in order to keep one type of data in each column<br />
- changed 'syn' to 'Synechococcus', 'picoeuk' to 'picoeukaryotes'<br />
- changed B1, B7, B12 to N+B1, N+B7, N+B12</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
BD Accuri C6 flow cytometer (Becton Dickerson and Company)
BD Accuri C6 flow cytometer (Becton Dickerson and Company)
PI Supplied Instrument Name: BD Accuri C6 flow cytometer (Becton Dickerson and Company) PI Supplied Instrument Description:Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company). Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: lab_Sanudo_2015
lab_Sanudo_2015
R/V Yellowfin
R/V Yellowfin
vessel
lab_Sanudo_2015
Sergio A. Sanudo-Wilhelmy
University of Southern California
R/V Yellowfin
R/V Yellowfin
vessel