http://lod.bco-dmo.org/id/dataset/726283
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-02-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Eukaryotic phytoplankton abundance and composition from nitrate and vitamin-B enriched treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project)
2018-02-05
publication
2018-02-05
revision
BCO-DMO Linked Data URI
2018-02-05
creation
http://lod.bco-dmo.org/id/dataset/726283
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Feixue Fu
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sanudo-Wilhelmy, S. A., Fu, F., Hutchins, D. A. (2018) Eukaryotic phytoplankton abundance and composition from nitrate and vitamin-B enriched treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-02-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/726283 [access date]
Eukaryote plankton abundance and composition with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015 Dataset Description: <p>This dataset includes&nbsp;abundance and percent composition for eukaryote phytoplankton collected in water samples&nbsp;from the San Pedro Ocean Time-series (SPOT), 2015. They were incubated with six treatments of nitrate and vitamin B.</p> Methods and Sampling: <p>Water samples were collected from 3 meters depth at the San Pedro Ocean Time-series (SPOT) station (33°33'N, 118°24'W) off the coast of Southern California in March 2015. Six treatments were used: control, nitrate, nitrate+B1, nitrate+B7, nitrate+B12, and nitrate+B1+B7+B12 with triplicate 10L incubations.&nbsp;Growth was tracked daily. Samples were collected initially, and at two points during the experiment: exponential growth and stationary phase. Exponential growth occurred at day 7 and stationary growth varied between treatment ranging from 10-12 days. The incubations were co-limited by nitrate and B12. Samples were flash frozen and stored at -80C until analysis. For further details on the methodology, see Suffridge et al (2017).</p>
<p>Samples of 50 ml volume were collected from each replicate of six treatments and preserved at 4°C in the dark with the addition of acidified Lugol’s solution an enumerated using an Accu-Scope 3032 inverted microscope.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1435666 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1435666
completed
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample_id
treatment
timepoint
taxon
organisms_ml
organisms_ml_sd
percent_composition
Accu-Scope 3032 inverted microscope
theme
None, User defined
sample identification
treatment
time_point
taxon
abundance
relative abundance
featureType
BCO-DMO Standard Parameters
CTD - profiler
Inverted Microscope
Niskin bottle
instrument
BCO-DMO Standard Instruments
lab_Sanudo_2015
service
Deployment Activity
near Los Angeles, California
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
https://osprey.bco-dmo.org/project/516960
Can the availability of B-vitamins control phyto-and-bacterioplankton successions in a coastal upwelling region?
<p><em>Description from NSF award abstract:</em><br />
B-vitamins (thiamin (B1), biotin (B7), and cobalamin (B12)) are organic molecules used by all organisms for many biochemical reactions ranging from DNA and amino acid synthesis to carbon dioxide assimilation. Despite their metabolic importance, many marine organisms cannot make them and need to obtain them from the environment. Because the requirement for a specific vitamin is different for different organisms, changes in the species composition of algae could be explained by their different B-vitamin requirements. For example, changes in the biological properties of waters during an algal bloom (removal of needed vitamins and release of other vitamins) may favor algae that require the vitamin released by the previous bloom (setting up a floral succession). This selective preconditioning of the waters may be one factor in the seasonal succession of algal species. However, evaluating the role of vitamins in marine ecology has been difficult. No study to date has been comprehensive enough to estimate the importance of vitamins in primary productivity and species succession. This is especially true in coastal upwelling regions that although relatively small in area, are orders of magnitude more productive than their open-ocean counterparts. In fact, those regions contribute a significant portion of the world fisheries. Therefore, in order to try to predict future changes in the world ocean due to human activity, the variables that influence or control the algal communities that dominate the very productive food chains of upwelling regions need to be identified.</p>
<p>This study will investigate how the availability of B-vitamins affects the dynamics of algal- and bacterioplankton population growth in coastal waters of an upwelling region off Southern California. This comprehensive field investigation will determine in situ temporal concentrations of several dissolved and particulate B-vitamins, inorganic micro- and macronutrients, concurrently with seasonal changes in phytoplankton and bacterial abundances and species composition at a long-term time series station within the San Pedro Basin near Los Angeles. Those measurements will be complemented with field incubation experiments with natural plankton assemblages to study the effect of organic and inorganic nutrient amendments on phytoplankton and bacterial community structure. This study will establish for the first time that the availability of ambient B-vitamins influence algal and bacterial species succession in a highly productive coastal upwelling region and that multiple and differing B-vitamin requirements limit growth of some phytoplankton species in those areas. Furthermore, this study will try to show that coastal upwelling transports some B-vitamins to the phytoplankton community in the photic zone from bacterially-influenced source waters within the upper mesopelagic zone.</p>
B-vitamin plankton succession
largerWorkCitation
project
eng; USA
biota
oceans
near Los Angeles, California
-118.3983
-118.3983
33.5478
33.5478
2015-03-11
2015-03-24
Southern California Bight
0
BCO-DMO catalogue of parameters from Eukaryotic phytoplankton abundance and composition from nitrate and vitamin-B enriched treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/726297.rdf
Name: sample_id
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/726298.rdf
Name: treatment
Units: unitless
Description: <p>treatments: control; nitrate (N); nitrate+B1 (N+B1); nitrate+B7 (N+B7); nitrate+B12 (N+B12); and nitrate+B1+B7+B12 (All)</p>
http://lod.bco-dmo.org/id/dataset-parameter/726299.rdf
Name: timepoint
Units: unitless
Description: <p>code for sampling period during one of three growth phases of the population: 0=initial sampling; 1=exponential phase; 2=stationary phase. Dates: 2015-03-12 (time 0); 2015-03-20 (time 1); 2015-03-25 (time 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/726300.rdf
Name: taxon
Units: unitless
Description: <p>taxon that was counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/726301.rdf
Name: organisms_ml
Units: individuals/milliliter
Description: <p>abundance of the organism/taxon</p>
http://lod.bco-dmo.org/id/dataset-parameter/726302.rdf
Name: organisms_ml_sd
Units: individuals/milliliter
Description: <p>standard deviation of abundance</p>
http://lod.bco-dmo.org/id/dataset-parameter/726303.rdf
Name: percent_composition
Units: unitless
Description: <p>relative abundance (percent) of each taxon in the sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2352
https://datadocs.bco-dmo.org/file/m77kmqzTPwpq9g/Vitacopss_taxon_abund.csv
Vitacopss_taxon_abund.csv
Primary data file for dataset ID 726283
download
https://osprey.bco-dmo.org/dataset/726283/data/download
download
onLine
dataset
<p>Water samples were collected from 3 meters depth at the San Pedro Ocean Time-series (SPOT) station (33°33'N, 118°24'W) off the coast of Southern California in March 2015. Six treatments were used: control, nitrate, nitrate+B1, nitrate+B7, nitrate+B12, and nitrate+B1+B7+B12 with triplicate 10L incubations.&nbsp;Growth was tracked daily. Samples were collected initially, and at two points during the experiment: exponential growth and stationary phase. Exponential growth occurred at day 7 and stationary growth varied between treatment ranging from 10-12 days. The incubations were co-limited by nitrate and B12. Samples were flash frozen and stored at -80C until analysis. For further details on the methodology, see Suffridge et al (2017).</p>
<p>Samples of 50 ml volume were collected from each replicate of six treatments and preserved at 4°C in the dark with the addition of acidified Lugol’s solution an enumerated using an Accu-Scope 3032 inverted microscope.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- added conventional header with dataset name, PI name, version date<br />
- renamed parameters to BCO-DMO standard<br />
- reduced decimal precision<br />
-&nbsp;changed proportions to percents</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Accu-Scope 3032 inverted microscope
Accu-Scope 3032 inverted microscope
PI Supplied Instrument Name: Accu-Scope 3032 inverted microscope PI Supplied Instrument Description:Used for cell counts: Samples of 50 ml volume were collected from each replicate of six treatments and preserved at 4°C in the dark with the addition of acidified Lugol’s solution an enumerated using an Accu-Scope 3032 inverted microscope. Instrument Name: Inverted Microscope Instrument Short Name: Instrument Description: An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: lab_Sanudo_2015
lab_Sanudo_2015
R/V Yellowfin
R/V Yellowfin
vessel
lab_Sanudo_2015
Sergio A. Sanudo-Wilhelmy
University of Southern California
R/V Yellowfin
R/V Yellowfin
vessel