http://lod.bco-dmo.org/id/dataset/726341
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-02-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Rates of primary and bacterial production measured in situ under ambient and elevated pCO2 (750 µatm) from the Hawaiian Ocean Time Series near Station ALOHA from 2010-2011.
2018-03-15
publication
2018-03-15
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-13
publication
https://doi.org/10.1575/1912/bco-dmo.726341.1
Matthew J. Church
University of Hawai'i
principalInvestigator
Ricardo Letelier
Oregon State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Church, M., Letelier, R. (2018) Rates of primary and bacterial production measured in situ under ambient and elevated pCO2 (750 µatm) from the Hawaiian Ocean Time Series near Station ALOHA from 2010-2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-15 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.726341.1 [access date]
array data 2010 and 2011 Dataset Description: <p>This data was used in Viviani et al (2018). For related research of experimental work done on some of the same cruises and drawn from some of the same experiments but reporting different parameters, see Bottjer et al (2014).</p> Methods and Sampling: <p>Rates of primary production were assessed using the 14C-bicarbonate incorporation technique. Rates of bacterial production were assessed using incorporation of 3H-leucine. Whole seawater samples from six discrete depths (5, 25, 45, 75, 100, and 125 m) were collected into duplicate acid-washed 20 L carboys. Control carboys were unamended; 43 mL of 1.0 N HCl and 4 mmol sodium bicarbonate were added to a treatment carboy at each depth, to increase the pCO2 to ~750 µatm, while minimizing changes to total alkalinity. Water from control and treatment carboys were then each subsampled into acid washed 500 mL polycarbonate bottles, with triplicate bottles per depth and treatment. To each bottle, was then added ~1.85 MBq 14C-bicarbonate. Water from each depth and treatment was also added to acid-cleaned 40 mL polycarbonate centrifuge tubes, each tube was then inoculated with 3H-leucine to a final concentration of 20 nmol L-1. For each depth and treatment, there was a dark (in a opaque cloth bag) and light incubation. Time zero blanks were immediately subsampled from each tube, by aliquoting 1.5 mL of seawater into 2 mL microcentrifuge tubes each containing 100 µL of 100% TCA. Following addition of radioactive substrates, the bottles and tubes were affixed to a free-drifting array and incubated&nbsp;<em>in situ</em>&nbsp;at the original depth of sample collection from dawn to dusk.</p>
<p>Upon recovery of the array, the total radioactivity added to each primary production sample bottle was determined by subsampling 250 µL aliquots of seawater into scintillation vials containing 500 µL of β-phenylethylamine. 400 mL from each 500 mL sample bottle was filtered at low vacuum (&lt;50 mm Hg) onto 25 mm diameter, 10 µm porosity polycarbonate membrane filters. The filtrate was collected and filtered onto 25 mm diameter 2 µm porosity polycarbonate membrane filters. 100 mL of that filtrate was then filtered onto 25 mm diameter 0.2 µm porosity polycarbonate membrane filters.&nbsp; Filters were stored frozen in 20 mL scintillation vials until analysis. Analysis consisted of acidification via addition of 1 mL of 2 N hydrochloric acid, and passively venting at least 24 hours in a fume hood to remove all inorganic 14C. Addition of 10 mL Ultima Gold LLT liquid scintillation cocktail and counting on a Perkin Elmer 2600 liquid scintillation counter completed the primary production analysis.</p>
<p>Upon recovery of the array, triplicate 1.5 mL subsamples were removed from each polycarbonate tube for bacterial production rate measurements, and aliquoted into 2 mL microcentrifuge tubes containing 100 µL of 100% TCA. The microcentrifuge tubes were frozen (-20°C) for subsequent processing, following the procedures described in Smith and Azam 1992.</p>
<p>Samples for the determination of dissolved inorganic carbon and total alkalinity were collected from each carboy and analyzed according to the protocols of the Hawaii Ocean Time-series (Dore et al. 2009; Winn et al. 1998). DIC and TA samples were collected into precombusted 300 mL borosilicate bottles. Care was taken to avoid introduction of air bubbles into samples during filling; bottles were allowed to overflow three times during filling. Once filled, samples were immediately fixed with 100 µL of a saturated solution of mercuric chloride; bottles were capped with a grease seal, and stored in the dark for later analysis.</p>
<p>Samples for measurement of fluorometric chlorophyll&nbsp;<em>a</em>&nbsp;were collected according to the protocols of the Hawaii Ocean Time-series; analysis was performed following Letelier et al. (1996).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850827 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850827
completed
Matthew J. Church
University of Hawai'i
(406) 982-3301
Flathead Lake Biological Station, University of Montana 321225 Bio Station Lane
Polson
MT
59860-6815
USA
matt.church@flbs.umt.edu
pointOfContact
Ricardo Letelier
Oregon State University
541-737 3890
College of Earth, Ocean, and Atmospheric Sciences (CEOAS) Oregon State University 104 CEOAS Admin. Bldg.
Corvallis
OR
97331-5503
USA
letelier@coas.oregonstate.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruise_id
station
cast
date
year
month
day
time
lat
lon
depth
PP_mean_10um
PP_std_dev_10um
PP_num_obs_10um
PP_mean_750uatm_pco2_10um
PP_std_dev_750uatm_pco2_10um
PP_num_obs_750uatm_pco2_10um
PP_mean_2um
PP_std_dev_2um
PP_num_obs_2um
PP_mean_750uatm_pco2_2um
PP_std_dev_750uatm_pco2_2um
PP_num_obs_750uatm_pco2_2um
PP_mean_0pt2um
PP_std_dev_0pt2um
PP_num_obs_0pt2um
PP_mean_750uatm_pco2_0pt2um
PP_std_dev_750uatm_pco2_0pt2um
PP_num_obs_750uatm_pco2_0pt2um
dissolved_inorganic_carbon
dissolved_inorganic_carbon_750uatm_pco2
total_alkalinity
total_alkalinity_750uatm_pco2
chlorophyll
leuc_3H_light_incorp_mean
leuc_3H_light_incorp_std_dev
leuc_3H_light_num_obs
leuc_3H_light_incorp_mean_750uatm_pco2
leuc_3H_light_incorp_std_dev_750uatm_pco2
leuc_3H_light_num_obs_750uatm_pco2
leuc_3H_dark_incorp_mean
leuc_3H_dark_incorp_std_dev
leuc_3H_dark_num_obs
leuc_3H_dark_incorp_mean_750uatm_pco2
leuc_3H_dark_incorp_std_dev_750uatm_pco2
leuc_3H_dark_num_obs_750uatm_pco2
Perkin Elmer 2600 liquid scintillation counter
theme
None, User defined
cruise id
station
cast
date
year
month of year
day of month
time of day
latitude
longitude
depth
primary production
standard deviation
numb_obs
dissolved inorganic Carbon
total alkalinity (TA)
chlorophyll a
leucine incorporation rate
featureType
BCO-DMO Standard Parameters
CTD - profiler
Liquid Scintillation Counter
instrument
BCO-DMO Standard Instruments
KM1110
KM1016
service
Deployment Activity
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Oceanic diazotroph community structure and activities in a high carbon dioxide world
https://osprey.bco-dmo.org/project/2102
Oceanic diazotroph community structure and activities in a high carbon dioxide world
<p>The North Pacific Subtropical Gyre (NPSG) is the largest ocean ecosystem on Earth, playing a prominent role in global carbon cycling and forming an important reservoir of marine biodiversity. Nitrogen (N2) fixing bacteria (termed diazotrophs) provide a major source of new nitrogen to the oligotrophic waters of the NPSG, thereby exerting direct control on the carbon cycle. Oceanic uptake of CO2 causes long-term changes in the partial pressure of CO2 (pCO2) in the seawater of this ecosystem. Therefore, understanding how carbon system perturbations may influence ocean biogeochemistry is an important and timely undertaking.</p>
<p>In this project, the investigators will examine how natural assemblages of N2 fixing microorganisms respond to perturbations in seawater carbon chemistry. Laboratory and field-based experiments will be placed in the context of monthly time series measurements on the activities and abundances of N2 fixing microorganism abundances. Together, the project will provide insight into the dependence of N2 fixing microorganism physiology on variations in CO2. The broad objectives of the research are: (1) Quantify the responses and consequences of changes in seawater pCO2 on the growth and community structure of naturally-occurring assemblages of ocean diazotrophs; (2) Identify why and how changes in seawater pCO2 influence the growth and carbon acquisition strategies of two model marine diazotrophs (Trichodesmium and Crocosphaera); and (3) Quantify temporal variability in diazotroph community structure and activities at Station ALOHA.</p>
<p>This is a Collaborative Research award.</p>
DIAZOTROPHS-CO2
largerWorkCitation
project
eng; USA
oceans
North Pacific Subtropical Gyre near Station ALOHA (22° 45'N, 158° 00'W)
-167.9648
-160.7528
22.7485
25.4193
2010-08-21
2011-03-16
0
BCO-DMO catalogue of parameters from Rates of primary and bacterial production measured in situ under ambient and elevated pCO2 (750 µatm) from the Hawaiian Ocean Time Series near Station ALOHA from 2010-2011.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/731046.rdf
Name: cruise_id
Units: no units
Description: <p>cruise identification number</p>
http://lod.bco-dmo.org/id/dataset-parameter/731047.rdf
Name: station
Units: text
Description: <p>station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/731048.rdf
Name: cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/731049.rdf
Name: date
Units: unitless
Description: <p>date sampling began; format: YYYYMMDD</p>
http://lod.bco-dmo.org/id/dataset-parameter/731050.rdf
Name: year
Units: unitless
Description: <p>year of sample; format: YYYY</p>
http://lod.bco-dmo.org/id/dataset-parameter/731051.rdf
Name: month
Units: unitless
Description: <p>month of sample; format: MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/731052.rdf
Name: day
Units: unitless
Description: <p>day of sample; format: DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/731053.rdf
Name: time
Units: unitless
Description: <p>time of sampling; format: hhmm</p>
http://lod.bco-dmo.org/id/dataset-parameter/731054.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/731055.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/731056.rdf
Name: depth
Units: meters
Description: <p>depth from which sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/731057.rdf
Name: PP_mean_10um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 10 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731058.rdf
Name: PP_std_dev_10um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 10 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731059.rdf
Name: PP_num_obs_10um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731060.rdf
Name: PP_mean_750uatm_pco2_10um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 10 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731061.rdf
Name: PP_std_dev_750uatm_pco2_10um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 10 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731062.rdf
Name: PP_num_obs_750uatm_pco2_10um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731063.rdf
Name: PP_mean_2um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 2 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731064.rdf
Name: PP_std_dev_2um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 2 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731065.rdf
Name: PP_num_obs_2um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731066.rdf
Name: PP_mean_750uatm_pco2_2um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 2 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731067.rdf
Name: PP_std_dev_750uatm_pco2_2um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 2 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731068.rdf
Name: PP_num_obs_750uatm_pco2_2um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731069.rdf
Name: PP_mean_0pt2um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 0.2 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731070.rdf
Name: PP_std_dev_0pt2um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 0.2 micron filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/731071.rdf
Name: PP_num_obs_0pt2um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731072.rdf
Name: PP_mean_750uatm_pco2_0pt2um
Units: micromol C/liter/day
Description: <p>mean 14C-Primary Production rate from 0.2 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731073.rdf
Name: PP_std_dev_750uatm_pco2_0pt2um
Units: micromol C/liter/day
Description: <p>standard deviation of 14C-Primary Production rate from 0.2 micron filters incubated at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731074.rdf
Name: PP_num_obs_750uatm_pco2_0pt2um
Units: unitless
Description: <p>number of samples used in calculation of PP rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731075.rdf
Name: dissolved_inorganic_carbon
Units: micromol/kilogram seawater
Description: <p>dissolved inorganic carbon of seawater used for PP and 3H_leuc incubations at ambient conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/731076.rdf
Name: dissolved_inorganic_carbon_750uatm_pco2
Units: micromol/kilogram seawater
Description: <p>dissolved inorganic carbon of seawater used for PP and 3H_leuc incubations at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731077.rdf
Name: total_alkalinity
Units: microequivalents/kilogram seawater
Description: <p>total alkalinity of seawater used for PP and 3H_leuc incubations at ambient conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/731078.rdf
Name: total_alkalinity_750uatm_pco2
Units: microequivalents/kilogram seawater
Description: <p>total alkalinity of seawater used for PP and 3H_leuc incubations at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731079.rdf
Name: chlorophyll
Units: micrograms/liter
Description: <p>chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/731080.rdf
Name: leuc_3H_light_incorp_mean
Units: picomol leucine/liter/hour
Description: <p>mean 3H-Leucine (light incubated) incorporation rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/731081.rdf
Name: leuc_3H_light_incorp_std_dev
Units: picomol leucine/liter/hour
Description: <p>standard deviation 3H-Leucine (light incubated) incorporation rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/731082.rdf
Name: leuc_3H_light_num_obs
Units: unitless
Description: <p>number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731083.rdf
Name: leuc_3H_light_incorp_mean_750uatm_pco2
Units: picomol leucine/liter/hour
Description: <p>mean 3H-Leucine (light incubated) incorporation rates at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731084.rdf
Name: leuc_3H_light_incorp_std_dev_750uatm_pco2
Units: picomol leucine/liter/hour
Description: <p>standard deviation 3H-Leucine (light incubated) incorporation rates at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731085.rdf
Name: leuc_3H_light_num_obs_750uatm_pco2
Units: unitless
Description: <p>number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731086.rdf
Name: leuc_3H_dark_incorp_mean
Units: picomol leucine/liter/hour
Description: <p>Mean 3H-Leucine (dark incubated) incorporation rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/731087.rdf
Name: leuc_3H_dark_incorp_std_dev
Units: picomol leucine/liter/hour
Description: <p>standard deviation 3H-Leucine (dark incubated) incorporation rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/731088.rdf
Name: leuc_3H_dark_num_obs
Units: unitless
Description: <p>number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/731089.rdf
Name: leuc_3H_dark_incorp_mean_750uatm_pco2
Units: picomol leucine/liter/hour
Description: <p>mean 3H-Leucine (dark incubated) incorporation rates at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731090.rdf
Name: leuc_3H_dark_incorp_std_dev_750uatm_pco2
Units: picomol leucine/liter/hour
Description: <p>standard deviation 3H-Leucine dark incubated) incorporation rates at 750 microatm pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/731091.rdf
Name: leuc_3H_dark_num_obs_750uatm_pco2
Units: unitless
Description: <p>number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6938
https://darchive.mblwhoilibrary.org/bitstream/1912/24269/1/dataset-726341_situ-pco2-manipulation-experiments-2010-2011__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.726341.1
download
onLine
dataset
<p>Rates of primary production were assessed using the 14C-bicarbonate incorporation technique. Rates of bacterial production were assessed using incorporation of 3H-leucine. Whole seawater samples from six discrete depths (5, 25, 45, 75, 100, and 125 m) were collected into duplicate acid-washed 20 L carboys. Control carboys were unamended; 43 mL of 1.0 N HCl and 4 mmol sodium bicarbonate were added to a treatment carboy at each depth, to increase the pCO2 to ~750 µatm, while minimizing changes to total alkalinity. Water from control and treatment carboys were then each subsampled into acid washed 500 mL polycarbonate bottles, with triplicate bottles per depth and treatment. To each bottle, was then added ~1.85 MBq 14C-bicarbonate. Water from each depth and treatment was also added to acid-cleaned 40 mL polycarbonate centrifuge tubes, each tube was then inoculated with 3H-leucine to a final concentration of 20 nmol L-1. For each depth and treatment, there was a dark (in a opaque cloth bag) and light incubation. Time zero blanks were immediately subsampled from each tube, by aliquoting 1.5 mL of seawater into 2 mL microcentrifuge tubes each containing 100 µL of 100% TCA. Following addition of radioactive substrates, the bottles and tubes were affixed to a free-drifting array and incubated&nbsp;<em>in situ</em>&nbsp;at the original depth of sample collection from dawn to dusk.</p>
<p>Upon recovery of the array, the total radioactivity added to each primary production sample bottle was determined by subsampling 250 µL aliquots of seawater into scintillation vials containing 500 µL of β-phenylethylamine. 400 mL from each 500 mL sample bottle was filtered at low vacuum (&lt;50 mm Hg) onto 25 mm diameter, 10 µm porosity polycarbonate membrane filters. The filtrate was collected and filtered onto 25 mm diameter 2 µm porosity polycarbonate membrane filters. 100 mL of that filtrate was then filtered onto 25 mm diameter 0.2 µm porosity polycarbonate membrane filters.&nbsp; Filters were stored frozen in 20 mL scintillation vials until analysis. Analysis consisted of acidification via addition of 1 mL of 2 N hydrochloric acid, and passively venting at least 24 hours in a fume hood to remove all inorganic 14C. Addition of 10 mL Ultima Gold LLT liquid scintillation cocktail and counting on a Perkin Elmer 2600 liquid scintillation counter completed the primary production analysis.</p>
<p>Upon recovery of the array, triplicate 1.5 mL subsamples were removed from each polycarbonate tube for bacterial production rate measurements, and aliquoted into 2 mL microcentrifuge tubes containing 100 µL of 100% TCA. The microcentrifuge tubes were frozen (-20°C) for subsequent processing, following the procedures described in Smith and Azam 1992.</p>
<p>Samples for the determination of dissolved inorganic carbon and total alkalinity were collected from each carboy and analyzed according to the protocols of the Hawaii Ocean Time-series (Dore et al. 2009; Winn et al. 1998). DIC and TA samples were collected into precombusted 300 mL borosilicate bottles. Care was taken to avoid introduction of air bubbles into samples during filling; bottles were allowed to overflow three times during filling. Once filled, samples were immediately fixed with 100 µL of a saturated solution of mercuric chloride; bottles were capped with a grease seal, and stored in the dark for later analysis.</p>
<p>Samples for measurement of fluorometric chlorophyll&nbsp;<em>a</em>&nbsp;were collected according to the protocols of the Hawaii Ocean Time-series; analysis was performed following Letelier et al. (1996).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Perkin Elmer 2600 liquid scintillation counter
Perkin Elmer 2600 liquid scintillation counter
PI Supplied Instrument Name: Perkin Elmer 2600 liquid scintillation counter Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Cruise: KM1110
KM1110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1110
Matthew J. Church
University of Hawai'i
http://dmoserv3.bco-dmo.org/jg/serv/BCO-DMO/DIAZOTROPHS_CO2/726342.html1%7Bdir=dmoserv3.whoi.edu/jg/dir/BCO-DMO/DIAZOTROPHS_CO2/,info=dmoserv3.bco-dmo.org/jg/info/BCO-DMO/DIAZOTROPHS_CO2/CO2_experimental%7D?cruise_id_eq_km1110
Report describing KM1110
Cruise: KM1016
KM1016
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1016
Matthew J. Church
University of Hawai'i
http://dmoserv3.bco-dmo.org/jg/serv/BCO-DMO/DIAZOTROPHS_CO2/726342.html1%7Bdir=dmoserv3.whoi.edu/jg/dir/BCO-DMO/DIAZOTROPHS_CO2/,info=dmoserv3.bco-dmo.org/jg/info/BCO-DMO/DIAZOTROPHS_CO2/CO2_experimental%7D?cruise_id_eq_km1016
Report describing KM1016
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel