http://lod.bco-dmo.org/id/dataset/726452
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-02-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)
2018-03-23
publication
2018-03-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-03
publication
https://doi.org/10.1575/1912/bco-dmo.726452.1
Janet E. Kubler
California State University Northridge
principalInvestigator
Steve Dudgeon
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.726452.1 [access date]
Plocamium growth and biomass Dataset Description: <p>This dataset includes measurements of frond area of <em>Plocamium cartilagineum</em> grown under various temperatures and CO2 levels. Biomass-specific growth and dynamic average of weekly growth is also calculated.</p>
<p><strong>Related Datasets:</strong><br />
<a href="https://www.bco-dmo.org/dataset/731187">Plocamium carbon nitrogen and stable isotopes</a>: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726664">Plocamium culture carbonate chemistry</a>: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731237">Plocamium culture: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731134">Plocamium cultures pH and temperature</a>: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731452">Plocamium exptl treatments summary</a>: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731213">Plocamium pigments</a>: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726480">Plocamium: pH drift</a>: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/733238">Rapid Light Curves_PAM</a>: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions</p> Methods and Sampling: <p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Areal growth: Frond pieces were placed under glass without overlap of branches adjacent to a ruler for scale and imaged using an Infinity 2 camera with attached macro lens mounted on a copy stand. Images and calibrated and surface area of fronds measured using Image J. Measurements were taken at the start and end of the trial. For all measures of growth (by surface area [adj_growth], and biomass [growth_biom] and [growth_dyn]) the calculations are specific growth rates, meaning LOG(measure@t2 / measure@t1)/(t2-t1).&nbsp;For surface area measures (adj_growth) and biomass (as growth_biom), t1 is the initial measure at the start of the experiment and t2 is the final measure at the end of the experiment. For biomass (asgrowth_dyn), t1 is the measure at the start of each weeklong interval and t2 is the measure at the end of the interval. Thus, there were typically 3 such intervals during a trial in which growth was measured and the datum in the column growth_dyn represents the average of those interval specific measures over the course of a trial.</p>
<p>Note: Since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.</p>
<p>Biomass: Frond pieces were gently blotted dry with paper toweling to remove surface water and weighed at weekly intervals to estimate biomass specific growth rates (growth_biom and growth_dyn). Note: since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316198
completed
Janet E. Kubler
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
janet.kubler@csun.edu
pointOfContact
Steve Dudgeon
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
steve.dudgeon@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
pot
pCO2
temp
trial
date_start
date_end
area_t0
area_end
days
adj_growth
growth_biom
growth_dyn
pCO2_target_range
Aqua Logic Cyclone Chiller
Lumenera Corp. Infinity 2 2.0 Megapixel Color CCD Camera, Navitar TV Zoom 7000 Macro lens
Qubit Systems Mass Flow Controllers (MFC)
Mettler Toledo AG204 Delta Range Analytical Balance
theme
None, User defined
sample identification
Partial pressure of CO2
water temperature at measurement depth
experiment id
date_start
date end
surface_area
days
growth
featureType
BCO-DMO Standard Parameters
Aquarium chiller
Camera
Mass Flow Controller
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
https://osprey.bco-dmo.org/project/2275
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
<p>Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, <em>Ulva</em> spp. and <em>Plocamium cartilagineum</em>, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in <em>Plocamium</em>, which relies solely on diffusive uptake of CO2, and populations of<em> Ulva</em> spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of<em> Ulva</em> spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on <em>Ulva</em> spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.</p>
Seaweed OA Resilience
largerWorkCitation
project
eng; USA
biota
oceans
-119
-118
33
34
2014-06-04
2015-02-23
Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
0
BCO-DMO catalogue of parameters from Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/726468.rdf
Name: pot
Units: unitless
Description: <p>culture pot identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/726469.rdf
Name: pCO2
Units: microatmospheres (uatm)
Description: <p>partial pressure of dissolved CO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/726470.rdf
Name: temp
Units: degrees Celsius
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/726471.rdf
Name: trial
Units: unitless
Description: <p>experimental trial consisting of 3 PCO2 levels with one always ~380 as control</p>
http://lod.bco-dmo.org/id/dataset-parameter/726472.rdf
Name: date_start
Units: unitless
Description: <p>start date for trial formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/726473.rdf
Name: date_end
Units: unitless
Description: <p>end date for trial formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/726474.rdf
Name: area_t0
Units: square centimeters (cm^2)
Description: <p>frond surface area on day 0 (start)</p>
http://lod.bco-dmo.org/id/dataset-parameter/726475.rdf
Name: area_end
Units: square centimeters (cm^2)
Description: <p>frond surface area at expt. end</p>
http://lod.bco-dmo.org/id/dataset-parameter/726476.rdf
Name: days
Units: days
Description: <p>Number of days for experimental trial</p>
http://lod.bco-dmo.org/id/dataset-parameter/726477.rdf
Name: adj_growth
Units: square centimeters/square centimeters/day (cm^2/cm^2/day)
Description: <p>Area-specific growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/726478.rdf
Name: growth_biom
Units: milligrams/gram/day (mg/g/day)
Description: <p>biomass-specific growth between t=0 and t=19 or 20 or 21 days</p>
http://lod.bco-dmo.org/id/dataset-parameter/726479.rdf
Name: growth_dyn
Units: milligrams/gram/day (mg/g/day)
Description: <p>dynamic average of weekly growth rates over the 3-4 week experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/769289.rdf
Name: pCO2_target_range
Units: unitless
Description: <p>Relative range for the target pCO2. See Supplemental Files for the table of culture conditions for each of the 8 trials.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5438
https://darchive.mblwhoilibrary.org/bitstream/1912/24186/1/dataset-726452_plocamium-growth-and-biomass__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.726452.1
download
onLine
dataset
<p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Areal growth: Frond pieces were placed under glass without overlap of branches adjacent to a ruler for scale and imaged using an Infinity 2 camera with attached macro lens mounted on a copy stand. Images and calibrated and surface area of fronds measured using Image J. Measurements were taken at the start and end of the trial. For all measures of growth (by surface area [adj_growth], and biomass [growth_biom] and [growth_dyn]) the calculations are specific growth rates, meaning LOG(measure@t2 / measure@t1)/(t2-t1).&nbsp;For surface area measures (adj_growth) and biomass (as growth_biom), t1 is the initial measure at the start of the experiment and t2 is the final measure at the end of the experiment. For biomass (asgrowth_dyn), t1 is the measure at the start of each weeklong interval and t2 is the measure at the end of the interval. Thus, there were typically 3 such intervals during a trial in which growth was measured and the datum in the column growth_dyn represents the average of those interval specific measures over the course of a trial.</p>
<p>Note: Since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.</p>
<p>Biomass: Frond pieces were gently blotted dry with paper toweling to remove surface water and weighed at weekly intervals to estimate biomass specific growth rates (growth_biom and growth_dyn). Note: since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- combined data from 3 submitted files: CO2trt_x_pot_trial.csv, Plo_areal_growth_CO2_Temp.csv, Plo_bio_growth_CO2_Temp.csv<br />
- added columns for date_start and date_end; moved&nbsp;trial&nbsp;to&nbsp;first&nbsp;column<br />
-&nbsp;reduced precision of area and growth columns from 7 to 2 decimal places and adj_growth from 9 to 5 places.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Aqua Logic Cyclone Chiller
Aqua Logic Cyclone Chiller
PI Supplied Instrument Name: Aqua Logic Cyclone Chiller PI Supplied Instrument Description:Used to control the temperature of the growth chambers. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
Lumenera Corp. Infinity 2 2.0 Megapixel Color CCD Camera, Navitar TV Zoom 7000 Macro lens
Lumenera Corp. Infinity 2 2.0 Megapixel Color CCD Camera, Navitar TV Zoom 7000 Macro lens
PI Supplied Instrument Name: Lumenera Corp. Infinity 2 2.0 Megapixel Color CCD Camera, Navitar TV Zoom 7000 Macro lens PI Supplied Instrument Description:To take images of fronds for area measurements. Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Qubit Systems Mass Flow Controllers (MFC)
Qubit Systems Mass Flow Controllers (MFC)
PI Supplied Instrument Name: Qubit Systems Mass Flow Controllers (MFC) PI Supplied Instrument Description:Used to mix gasses: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
Mettler Toledo AG204 Delta Range Analytical Balance
Mettler Toledo AG204 Delta Range Analytical Balance
PI Supplied Instrument Name: Mettler Toledo AG204 Delta Range Analytical Balance PI Supplied Instrument Description:To measure biomass of fronds Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/