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            <gco:CharacterString>Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-02-07 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.726480.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Carbonate chemistry over a time-course in pH drift Dataset Description: &amp;lt;p&amp;gt;This dataset includes measurements of pH drift of seawater samples taken over 30-hour time periods in the presence or absence of &amp;lt;em&amp;gt;Plocamium cartilagineum&amp;lt;/em&amp;gt;, with two carbonic anhydrase inhibitors, and with three pCO2 levels. Carbonate chemistry is reported at six time intervals.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Related Datasets:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/731187&amp;quot;&amp;gt;Plocamium carbon nitrogen and stable isotopes&amp;lt;/a&amp;gt;: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/726664&amp;quot;&amp;gt;Plocamium culture carbonate chemistry&amp;lt;/a&amp;gt;: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/731237&amp;quot;&amp;gt;Plocamium culture: seawater delta13C&amp;lt;/a&amp;gt;: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/731134&amp;quot;&amp;gt;Plocamium cultures pH and temperature&amp;lt;/a&amp;gt;: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/731452&amp;quot;&amp;gt;Plocamium exptl treatments summary&amp;lt;/a&amp;gt;: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/726452&amp;quot;&amp;gt;Plocamium growth and biomass&amp;lt;/a&amp;gt;: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/731213&amp;quot;&amp;gt;Plocamium pigments&amp;lt;/a&amp;gt;: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/733238&amp;quot;&amp;gt;Rapid Light Curves_PAM&amp;lt;/a&amp;gt;: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;em&amp;gt;Plocamium &amp;lt;/em&amp;gt;&amp;lt;em&amp;gt;cartilagineum&amp;lt;/em&amp;gt;&amp;amp;nbsp;was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seawater samples were collected over a 30-hour time course of pH drift experiments with &amp;lt;em&amp;gt;Plocamium &amp;lt;/em&amp;gt;&amp;lt;em&amp;gt;cartilagineum&amp;lt;/em&amp;gt; to test mechanism of carbon acquisition by alga and the effect on carbonate chemistry parameters reported in these datasets. Samples were collected at 0, 2, 6, 20, 24 and 30 hours and parameters of carbonate chemistry were measured (DIC,CO2, HCO3,CO3-described below). pH drift assay experiments consisted of 2 independent variables; inhibitor treatment and algal presence, with 3 and 2 levels, respectively for each independent variable (see parameters section). Two replicate water samples were collected from each of 9 experimental culture pots of algae grown for 3 weeks at different combinations of pCO2 and temperature during each of 7 experimental trials.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Methodology Reference:&amp;lt;br /&amp;gt;
Dickson AG, Sabine CL, Christian JR (2007). Guide to Best Practices for Ocean CO2 Measurements.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55177.rdf" xlink:title="OCE-1316198" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316198</gmx:Anchor>
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Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
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Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
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Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
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                <gco:CharacterString>&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;Plocamium &amp;lt;/em&amp;gt;&amp;lt;em&amp;gt;cartilagineum&amp;lt;/em&amp;gt;&amp;amp;nbsp;was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seawater samples were collected over a 30-hour time course of pH drift experiments with &amp;lt;em&amp;gt;Plocamium &amp;lt;/em&amp;gt;&amp;lt;em&amp;gt;cartilagineum&amp;lt;/em&amp;gt; to test mechanism of carbon acquisition by alga and the effect on carbonate chemistry parameters reported in these datasets. Samples were collected at 0, 2, 6, 20, 24 and 30 hours and parameters of carbonate chemistry were measured (DIC,CO2, HCO3,CO3-described below). pH drift assay experiments consisted of 2 independent variables; inhibitor treatment and algal presence, with 3 and 2 levels, respectively for each independent variable (see parameters section). Two replicate water samples were collected from each of 9 experimental culture pots of algae grown for 3 weeks at different combinations of pCO2 and temperature during each of 7 experimental trials.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Methodology Reference:&amp;lt;br /&amp;gt;
Dickson AG, Sabine CL, Christian JR (2007). Guide to Best Practices for Ocean CO2 Measurements.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Lavigne H, Epitalon, JM, Gattuso JP, 2011. Seacarb: seawater carbonate chemistry with R.&amp;lt;br /&amp;gt;
R package version 3.0. http://CRAN.R-project.org/package=seacarb&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Processing Notes:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
- added conventional header with dataset name, PI name, version date&amp;lt;br /&amp;gt;
- modified parameter names to conform with BCO-DMO naming conventions&amp;lt;br /&amp;gt;
-&amp;amp;nbsp;combined data from the 8 submitted trial files&amp;lt;/p&amp;gt;</gco:CharacterString>
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