<div><p><strong>Sampling and Analytical Methodology: </strong></p>
<p>Experiments were conducted between July 2011 and April 2013 during five research cruises to Station ALOHA (22.75°N, 158°W), the well-characterized study site of the Hawaii Ocean Time-series (HOT) program. Sampling occurred during four HOT cruises and one Center for Microbial Oceanography: Research and Education (C-MORE) cruise (termed HOE-DYLAN 5) aboard the R/V Kilo Moana. Seawater was collected in 12 L polyvinylchloride bottles affixed to a 24-bottle rosette sampler equipped with a Sea-Bird 911+ conductivity, temperature, and depth profiler. Nine 20-L polycarbonate carboys were filled with 25 m Station ALOHA seawater pre-filtered off the rosette sampler through a Nitex mesh (pore size ~202 μm) to exclude larger zooplankton. Of these, 3 carboys received additions of nitrate (target ~2.8 μM N final concentration as NaNO<span style="font-family:inherit; font-size:inherit">3</span>) and three carboys received additions of ammonium (target ~2.8 μM N final concentration as NH<span style="font-family:inherit; font-size:inherit">4</span>Cl). All carboys, including three ‘Control’ carboys, received additions of phosphate (target ~0.2 μM P final concentration as KH<span style="font-family:inherit; font-size:inherit">2</span>PO<span style="font-family:inherit; font-size:inherit">4</span>) and silicic acid (target ~2.8 μM Si final concentration as Na<span style="font-family:inherit; font-size:inherit">2</span>SiO<span style="font-family:inherit; font-size:inherit">3</span>) to achieve a final N:P:Si stoichiometric ratio (14:1:14). Carboys were incubated for 120 to 144 hours and subsampled at approximately daily scales throughout the experiment (Table 1). All sampling was conducted before sunrise in order to allow productivity rate measurements to span the full photoperiod.</p>
<p>Seawater samples (2 mL) for photosynthetic picoeukaryote cell abundance measurements were collected for each experiment into cryotubes (Corning) containing 30 µl of 16% paraformaldehyde for a final concentration of 0.24% (w/v), kept for 15 minutes in the dark, flash-frozen in liquid nitrogen, and stored at -80°C until analyzed. Photosynthetic picoeukaryote cells were distinguished using a BD InfluxTM flow cytometer (triggered on forward scatter) with the data acquisition software Spigot. Cells were enumerated based on forward scatter, side scatter, chlorophyll-based red fluorescence (692 ± 20 nm), and phycoerythrin-based orange fluorescence (585 ± 20 nm) on two lasers, 488 nm and 457 nm. Cell counts were determined using the data analysis software FlowJo 10.0.7.</p></div>
photosynthetic picoplankton abundances
<div><p>These data have been submitted to BCO-DMO and are in the process of being served.</p>
<p>These data contain photosynthetic picoplankton abundances from nitrogen perturbation experiments conducted at Station ALOHA using 25 m seawater during HOT and CMORE cruises between 2011 and 2013. </p></div>
Perturbation experiments: FCM
728072
Perturbation experiments: FCM
2018-02-20T16:01:40-05:00
2018-02-20T16:01:40-05:00
2018-02-20T16:07:56-05:00
urn:bcodmo:dataset:728072
Photosynthetic picoplankton abundances from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)
false
Church, M. J., Kolber, Z., Zehr, J. P., Arrigo, K. R. () Photosynthetic picoplankton abundances from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/728072 [access date]
true
false
HTML
https://www.bco-dmo.org/dataset/728072
text/html
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/728072/datapackage.json
application/vnd.datapackage+json
PDF
https://www.bco-dmo.org/dataset/728072/Dataset_description.pdf
application/pdf
JSON-LD
https://www.bco-dmo.org/dataset/728072.json
application/ld+json
Turtle
https://www.bco-dmo.org/dataset/728072.ttl
text/turtle
RDF/XML
https://www.bco-dmo.org/dataset/728072.rdf
application/rdf+xml
ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/728072/iso
application/xml
http://www.isotc211.org/2005/gmd-noaa
Dublin Core
https://www.bco-dmo.org/dataset/728072/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
728072
http://lod.bco-dmo.org/id/dataset/728072