http://lod.bco-dmo.org/id/dataset/729480
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-03-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Measurements of dissolved organic nitrogen concentration and d15N from R/V Atlantis and R/V Melville cruises in the Eastern Tropical South Pacific from 2010 to 2011.
2018-03-06
publication
2018-03-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-19
publication
https://doi.org/10.1575/1912/bco-dmo.729480.1
Angela N. Knapp
Florida State University EOAS
principalInvestigator
Karen L. Casciotti
Stanford University
principalInvestigator
Maria Prokopenko
Pomona College
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Knapp, A., Casciotti, K., Prokopenko, M. (2018) Measurements of dissolved organic nitrogen concentration and d15N from R/V Atlantis and R/V Melville cruises in the Eastern Tropical South Pacific from 2010 to 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-06 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.729480.1 [access date]
Measurements of dissolved organic nitrogen concentration and d15N Dataset Description: <p>Measurements of dissolved organic nitrogen concentration and d15N.</p> Methods and Sampling: <p><strong>NO3- + NO2- concentration and isotopic composition analysis</strong></p>
<p>The NO3- + NO2- concentration of samples was determined using chemiluminescent analysis (Braman &amp; Hendrix, 1989) in a configuration with a detection limit of 0.05 uM, and +/- 0.1 uM for 1 standard deviation (S.D.). The d15N of NO3- + NO2- was determined using the “denitrifier” method (K. L. Casciotti, Sigman, Hastings, Bohlke, &amp; Hilkert, 2002; Sigman et al., 2001) with modifications (McIlvin &amp; Casciotti, 2011) on samples with NO3- + NO2- concentration &gt;0.3 uM (typically &lt;0.2‰ 1 S.D.) (Supp. Table 1) (Knapp, Casciotti, Berelson, Prokopenko, &amp; Capone, 2016).</p>
<p><strong>DON concentration and isotopic analysis</strong></p>
<p>The DON concentration of samples was determined using persulfate oxidation to convert DON to NO3- (Solorzano &amp; Sharp, 1980), adapted according to (Knapp et al., 2005). The resulting NO3- concentration was then measured using chemiluminescence as described above. In cases where NO3- + NO2- (and/or ammonium, NH4+) was above the detection limit, DON was determined by subtracting the concentration of NO3- + NO2-+NH4+ from the concentration of total dissolved N (TDN). The average standard deviation for duplicate DON concentration analyses of individual samples that have undetectable levels of NO3- in the sample was +/- 0.30 uM, and the propagated error for DON concentration in the presence of detectable NO3- was +/- 0.32 uM.</p>
<p>The d15N of DON was determined according to (Knapp et al., 2005), where DON samples were oxidized to NO3- by persulfate oxidation (as described above in section 2.2), acidified to a pH range of 3 to 4, and measured as NO3- by the denitrifier method. In samples with measurable NO3- + NO2-, the d15N of DON is calculated by mass balance by subtracting the NO3- + NO2- concentration and d15N of NO3- + NO2- from the TDN concentration and TDN d15N measurements. In surface samples with undetectable NO3- + NO2- concentration, the standard deviation of duplicate analyses of DON d15N in a sample is +/- 0.3‰. For subsurface samples with NO3- + NO2- concentration approximately equal to the DON concentration, the propagated error for the calculation of DON d15N using a Monte Carlo method (Press, Teukolsky, Vetterling, &amp; Flannery, 1992), and assuming duplicate analysis of a single sample and the standard deviations for TN concentration, NO3- + NO2- concentration and d15N of NO3- + NO2- given above, is +/- 0.6 0/00. The 15N of DON in samples with NO3- + NO2- concentration exceeding DON concentration, and/or with NH4+ concentration &gt; 0.2 uM, was not determined (i.e., Stations 9, 10, 11, and 12 from the 2010 cruise).</p>
<p><strong>Sampling</strong></p>
<p>Samples were collected on the R/V Atlantis in January through February 2010, and the R/V Melville in March through April 2011 between 10 and 20 ºS and 80º W and 100º W (Fig. 1), with station locations and sample depths, salinities, sigma theta values, chlorophyll a concentrations, nitrate+nitrite concentration, NO3-+ NO2- d15N, DON concentrations, and DON d15N reported in Supplementary Information Table 1. Water column samples were collected by Niskin bottles deployed on a rosette equipped with conductivity-temperature-depth (CTD) sensors. All samples were collected into acid-washed, sample-rinsed HDPE bottles, and samples from the upper 400 m passed a 0.2 um filter before collection, and were stored at -20º C until analysis on land.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850801 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850801
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850905 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850905
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961207 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961207
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961098 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961098
completed
Angela N. Knapp
Florida State University EOAS
(979) 862-5235
797 Lamar St. Dept of Oceanography, Eller O&M Room 1204
College Station
TX
77843
USA
aknapp@tamu.edu
pointOfContact
Karen L. Casciotti
Stanford University
650-721-5545
Department of Environmental Earth System Science 473 Via Ortega, Y2E2 Building, Room 140
Stanford
CA
94305
USA
kcasciotti@stanford.edu
pointOfContact
Maria Prokopenko
Pomona College
323-630-4068
185 E Sixth St
Claremont
CA
91711-4434
US
Maria.Prokopenko@pomona.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
deployment
cruise_year
date
station
lat
lon
depth
NO3_NO2
NO3_NO2_d15N
DON
DON_stdev
DON_d15N
DON_d15N_stdev
CTD
Sievers 280i Nitric Oxide Analyzer
Teledyne API Model 200EU Chemiluminescence NO/NOx/NOX analyzer
Thermo Delta V Plus isotope ratio mass spectrometer
Niskin
theme
None, User defined
deployment number
year
date
station
latitude
longitude
depth
nitrate plus nitrite
d15N_NO3
Dissolved Organic Nitrogen
d15N
featureType
BCO-DMO Standard Parameters
CTD - profiler
Gas Analyzer
Gas Analyzer
Isotope-ratio Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
AT15-61
MV1104
service
Deployment Activity
Eastern Tropical South Pacific
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Documenting N2 fixation in N deficient waters of the Eastern Tropical South Pacific
https://osprey.bco-dmo.org/project/555496
Collaborative Research: Documenting N2 fixation in N deficient waters of the Eastern Tropical South Pacific
<p><em>Description from NSF award abstract:</em><br />
Several independent lines of geochemical and remote sensing evidence suggest that dinitrogen (N2) fixation may be associated with surface waters downstream of major oxygen minimum zones (OMZs) and in particular in the Eastern Tropical South Pacific (ETSP). However, little direct evidence supports these inferences. Besides substantiating these indirect assessments, documenting significant N2 fixation in the ETSP would provide insight into two longstanding controversies: Is the marine N budget balanced, as implied by modeling and paleoceanographic data, and if so, how are the processes that add and remove N spatially, and thus temporally coupled?</p>
<p>In this project researchers at the University of Southern California and the University of Miami will test the hypothesis that fixation occurs in the ETSP at areal rates that equal or exceed those previously documented in more well-studied regions such as the oligotrophic waters of the sub/tropical North Atlantic. If scaled to the surface area of ETSP waters, this could add an additional 10-50 Tg N per year of inputs to the global marine N budget. They will undertake two cruises in the ETSP during early and late summer in two consecutive years to assess the quantitative significance of N2 fixation as a source of new N to surface waters using complementary biological and geochemical tools. N2 fixation rates will be evaluated on two temporal/spatial scales: daily/local (bottle 15N2 incubations and floating sediment traps); and seasonal/regional (d15N budget using moored sediment traps and water column TDN d15N). These estimates provide detailed observations of potential N2 fixation during station occupation in two summer seasons, when rates are expected to be greatest, as well as prolonged observation over lower expected N2 fixation periods. A combination of these different estimates will aim to determine if N2 fixation in this region can help balance the marine N budget. If all goes as planned, this study will determine the quantitative importance of N2 fixation in the ETSP, and whether these previously undocumented rates can help resolve the marine N budget. Implications include the ability of the marine N cycle to maintain homeostasis, and thus the global C cycle on glacial/interglacial time scales.</p>
N2 fixation ETSP
largerWorkCitation
project
Expression of Microbial Nitrification in the Stable Isotopic Systematics of Oceanic Nitrite and Nitrate
https://osprey.bco-dmo.org/project/555516
Expression of Microbial Nitrification in the Stable Isotopic Systematics of Oceanic Nitrite and Nitrate
<p><em>Description from NSF award abstract:</em><br />
Closing the marine budgets of nitrate and nitrous oxide are central goals for researchers interested in nutrient-driven changes in primary productivity and climate change. With the implementation of new methods for oxygen isotopic analysis of seawater nitrate, it will be possible to construct a budget for nitrate based on its oxygen isotopic distribution that is complementary to nitrogen isotope budgets. Before we can effectively use oxygen isotopes in nitrate to inform the current understanding of the marine nitrogen cycle, we must first understand how different processes that produce (nitrification) and consume (assimilation, denitrification) nitrate affect its oxygen isotopic signature.</p>
<p>In this study, researchers at the Woods Hole Oceanographic Institution will provide a quantitative assessment of the oxygen isotopic systematics of nitrification in the field and thus fill a key gap in our understanding of 18O variations in nitrate, nitrite, and nitrous oxide. The primary goal is to develop a quantitative prediction of the oxygen isotopic signatures of nitrite and nitrate produced during nitrification in the sea. The researchers hypothesize that oxygen isotopic fractionation during nitrification is the primary factor setting the 18O values of newly produced nitrate and nitrite. Secondly, they hypothesize that oxygen atom exchange is low where ammonia oxidation and nitrite oxidation are tightly coupled, but may increase in regions with nitrite accumulation, such as in the primary and secondary nitrite maxima. They will test these hypotheses with a series of targeted laboratory and field experiments, as well as with measurements of nitrite and nitrate isotopic distributions extending through the euphotic zone, primary nitrite maximum, and secondary nitrite maximum of the Eastern Tropical South Pacific. The results of these experiments are expected to provide fundamental information required for the interpretation of 18O isotopic signatures in nitrite, nitrate, and N2O in the context of underlying microbial processes. A better understanding of these features and the processes involved is important for quantifying new production, controls on the N budget, and N2O production in the ocean -- which should lead to a better understanding of the direct and indirect interactions among the nitrogen cycle, marine chemistry, and climate.</p>
Microbial Nitrification
largerWorkCitation
project
eng; USA
oceans
Eastern Tropical South Pacific
-101.3576
-80
-20
-9.3059
2010-02-01
2011-04-18
Eastern Tropical South Pacific
0
BCO-DMO catalogue of parameters from Measurements of dissolved organic nitrogen concentration and d15N from R/V Atlantis and R/V Melville cruises in the Eastern Tropical South Pacific from 2010 to 2011.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/729504.rdf
Name: deployment
Units: unitless
Description: <p>Deployment name</p>
http://lod.bco-dmo.org/id/dataset-parameter/729505.rdf
Name: cruise_year
Units: unitless
Description: <p>Year of cruise; yyyy</p>
http://lod.bco-dmo.org/id/dataset-parameter/729506.rdf
Name: date
Units: unitless
Description: <p>Date of sampling; yyyy/mm/dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/729507.rdf
Name: station
Units: unitless
Description: <p>Station where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/729508.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/729509.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/729510.rdf
Name: depth
Units: meters
Description: <p>Depth of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/729511.rdf
Name: NO3_NO2
Units: uM
Description: <p>NO3- + NO2- values</p>
http://lod.bco-dmo.org/id/dataset-parameter/729512.rdf
Name: NO3_NO2_d15N
Units: ppm vs air
Description: <p>No3- + NO2- d15N values</p>
http://lod.bco-dmo.org/id/dataset-parameter/729513.rdf
Name: DON
Units: uM
Description: <p>Dissolved organic nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/729514.rdf
Name: DON_stdev
Units: uM
Description: <p>Standard deviation of dissolved organic nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/729515.rdf
Name: DON_d15N
Units: ppm vs air
Description: <p>Dissolved organic nitrogen d15N</p>
http://lod.bco-dmo.org/id/dataset-parameter/729516.rdf
Name: DON_d15N_stdev
Units: ppm vs air
Description: <p>Standard deviation of dissolved organic nitrogen d15N</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5634
https://darchive.mblwhoilibrary.org/bitstream/1912/23873/1/dataset-729480_don-concentration-and-don-d15n__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.729480.1
download
onLine
dataset
<p><strong>NO3- + NO2- concentration and isotopic composition analysis</strong></p>
<p>The NO3- + NO2- concentration of samples was determined using chemiluminescent analysis (Braman &amp; Hendrix, 1989) in a configuration with a detection limit of 0.05 uM, and +/- 0.1 uM for 1 standard deviation (S.D.). The d15N of NO3- + NO2- was determined using the “denitrifier” method (K. L. Casciotti, Sigman, Hastings, Bohlke, &amp; Hilkert, 2002; Sigman et al., 2001) with modifications (McIlvin &amp; Casciotti, 2011) on samples with NO3- + NO2- concentration &gt;0.3 uM (typically &lt;0.2‰ 1 S.D.) (Supp. Table 1) (Knapp, Casciotti, Berelson, Prokopenko, &amp; Capone, 2016).</p>
<p><strong>DON concentration and isotopic analysis</strong></p>
<p>The DON concentration of samples was determined using persulfate oxidation to convert DON to NO3- (Solorzano &amp; Sharp, 1980), adapted according to (Knapp et al., 2005). The resulting NO3- concentration was then measured using chemiluminescence as described above. In cases where NO3- + NO2- (and/or ammonium, NH4+) was above the detection limit, DON was determined by subtracting the concentration of NO3- + NO2-+NH4+ from the concentration of total dissolved N (TDN). The average standard deviation for duplicate DON concentration analyses of individual samples that have undetectable levels of NO3- in the sample was +/- 0.30 uM, and the propagated error for DON concentration in the presence of detectable NO3- was +/- 0.32 uM.</p>
<p>The d15N of DON was determined according to (Knapp et al., 2005), where DON samples were oxidized to NO3- by persulfate oxidation (as described above in section 2.2), acidified to a pH range of 3 to 4, and measured as NO3- by the denitrifier method. In samples with measurable NO3- + NO2-, the d15N of DON is calculated by mass balance by subtracting the NO3- + NO2- concentration and d15N of NO3- + NO2- from the TDN concentration and TDN d15N measurements. In surface samples with undetectable NO3- + NO2- concentration, the standard deviation of duplicate analyses of DON d15N in a sample is +/- 0.3‰. For subsurface samples with NO3- + NO2- concentration approximately equal to the DON concentration, the propagated error for the calculation of DON d15N using a Monte Carlo method (Press, Teukolsky, Vetterling, &amp; Flannery, 1992), and assuming duplicate analysis of a single sample and the standard deviations for TN concentration, NO3- + NO2- concentration and d15N of NO3- + NO2- given above, is +/- 0.6 0/00. The 15N of DON in samples with NO3- + NO2- concentration exceeding DON concentration, and/or with NH4+ concentration &gt; 0.2 uM, was not determined (i.e., Stations 9, 10, 11, and 12 from the 2010 cruise).</p>
<p><strong>Sampling</strong></p>
<p>Samples were collected on the R/V Atlantis in January through February 2010, and the R/V Melville in March through April 2011 between 10 and 20 ºS and 80º W and 100º W (Fig. 1), with station locations and sample depths, salinities, sigma theta values, chlorophyll a concentrations, nitrate+nitrite concentration, NO3-+ NO2- d15N, DON concentrations, and DON d15N reported in Supplementary Information Table 1. Water column samples were collected by Niskin bottles deployed on a rosette equipped with conductivity-temperature-depth (CTD) sensors. All samples were collected into acid-washed, sample-rinsed HDPE bottles, and samples from the upper 400 m passed a 0.2 um filter before collection, and were stored at -20º C until analysis on land.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<p>- dates reformatted to yyyy/mm/dd<br />
- no data replaced with nd<br />
- N/A replaced with NA<br />
- blanks replaced with nd<br />
- converted from wide to long format</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD
CTD
PI Supplied Instrument Name: CTD Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Sievers 280i Nitric Oxide Analyzer
Sievers 280i Nitric Oxide Analyzer
PI Supplied Instrument Name: Sievers 280i Nitric Oxide Analyzer PI Supplied Instrument Description:Used to collect the NO3- + NO2- concentration and DON concentration data Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
Teledyne API Model 200EU Chemiluminescence NO/NOx/NOX analyzer
Teledyne API Model 200EU Chemiluminescence NO/NOx/NOX analyzer
PI Supplied Instrument Name: Teledyne API Model 200EU Chemiluminescence NO/NOx/NOX analyzer PI Supplied Instrument Description:Used to collect the NO3- + NO2- concentration and DON concentration data Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
Thermo Delta V Plus isotope ratio mass spectrometer
Thermo Delta V Plus isotope ratio mass spectrometer
PI Supplied Instrument Name: Thermo Delta V Plus isotope ratio mass spectrometer PI Supplied Instrument Description:Used to collect NO3- + NO2- d15N and DON d15N data Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Niskin
Niskin
PI Supplied Instrument Name: Niskin PI Supplied Instrument Description:Used to collect water samples Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AT15-61
AT15-61
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT15-61
Douglas G. Capone
University of Southern California
Cruise: MV1104
MV1104
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
MV1104
William M. Berelson
University of Southern California
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel