http://lod.bco-dmo.org/id/dataset/730722
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-03-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Total spectral count of proteins from R/V Kilo Moana cruise KM1128 for the METZYME expedition in the tropical North Pacific in 2011.
2022-06-06
publication
2022-06-06
revision
BCO-DMO Linked Data URI
2022-06-06
creation
http://lod.bco-dmo.org/id/dataset/730722
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saito, M. A. (2022) Total spectral count of proteins from R/V Kilo Moana cruise KM1128 for the METZYME expedition in the tropical North Pacific in 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 5) Version Date 2022-06-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/730722 [access date]
Global metaproteomic dataset for KM1128 the METZYME expedition. Dataset Description: <p>These data are part of the Ocean Protein Portal "MetZyme 0.2" dataset version 5 (https://proteinportal.whoi.edu/; Saito et al., 2020).</p> Methods and Sampling: <p>Ocean metaproteomes were sampled and extracted as described in Saito et al., 2014. The protocols are reproduced here:</p>
<p>Samples were collected during the KM1128 METZYME research expedition (Metals and Enzymes in the Pacific) on the R/V Kilo Moana October 1-25, 2011 from Oahu, Hawaii to Apia, Samoa, with Carl Lamborg and Mak Saito as Chief Scientists. Microbial biomass for protein analyses was collected on vertical profiles using in situ high volume particle filtration pumps with a focus on the North Pacific and Equatorial regions. Protein samples were collected by a suite of 4 L/min and 8 L/min McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA) outfitted with custom Mini-MULVS multiple filter head systems. The 0.2-3.0 micron size fraction was collected on 142 mm filters (Supor, Pall Corp.) for analyses used in this study. The volume of water was pumped until a minimal flow rate was achieved or the allotted cast time period expired, typically ~300-500 L. Filters were sectioned immediately after pump retrieval, and protein samples (¼ filter) were stored in RNAlater reagent (Ambion, Life Technologies), which has been shown to be an effective preservative for cyanobacterial biomass (Saito et al., 2011), frozen at -80C, transported back to the laboratory on dry ice, and stored at -80C until analysis.</p>
<p>Total microbial protein (0.2-3.0um fraction) was extracted using detergent-based methods, described below. Total protein showed enhanced concentrations in the photic zone, particularly in the Equatorial and South Pacific portions of the transect (Fig. S1F). For protein extraction, samples were thawed and the filter and RNAlater preservative (Ambion Life Technologies) were separated. The removed preservative was spin-concentrated by a 5K MWCO membrane (Sartorius Stedim Biotech 6 mL, 5 K MWCO Vivaspin units; Goettingen, Germany), and rinsed with 0.1M Tris buffer to recover, desalt, and concentrate any suspended material. The sample filter was unfolded and placed in a larger tube to which 1% SDS extraction buffer (1% SDS, 0.1M Tris/HCL pH 7.5, 10mM EDTA) and the rinsed/desalted RNAlater fraction was added back. Each sample was incubated at room temperature for 15 minutes, heated at 95C for 10 minutes, and shaken at room temperature for 350 rpm for 1 h. The protein extract was decanted and placed in a new tube and centrifuged for 30 min x 3220 g at room temperature. The supernatants were removed and filtered through a 5 um low protein binding syringe filter (Fisher Scientific), and the filter rinsed with extraction buffer. The extracts were concentrated by 5 kD membrane centrifugation to a small volume, washed with extraction buffer, and concentrated again. Each sample was precipitated with cold 50% methanol (MeOH) 50% acetone 0.5 mM HCL for 3 days at –20C, centrifuged at 14100 x g (14500 rpm) for 30 m at 4C, decanted and dried by vacuum concentration (Thermo Savant Speedvac) for 10 min or until dry. Pellets were resuspended in 1% SDS extraction buffer and left at room temperature (RT) for 1 h to redissolve. Total protein was quantified (Bio-Rad DC protein assay, Hercules, CA) with BSA as a standard.</p>
<p>Extracted proteins were purified from SDS detergent, reduced, alkylated, and&nbsp; trypsin digested, while embedded within a polyacrylamide tube gel, using a modified protocol from a previously published method (38). The tube gel approach allowed all proteins including membrane proteins to be solubilized by detergent and purified while immobilized in the gel matrix. A gel premix was made by combining 1 M Tris HCL (pH 7.5) and 40% Bis-acrylimide L 29:1 (Acros Organics) at a ratio of 1:3. The premix (103 ul) was combined with an extracted protein sample (usually 25 ug-200 ug), TE, 7 ul 1% APS and 3 ul of TEMED (Acros Organics) to a final volume of 200 ul. After 1 h of polymerization at RT, 200 ul of gel fix solution (50% ETOH, 10% acetic acid in LC/MS grade water) was added to the top of the gel and incubated at RT for 20 minutes. Liquid was then removed and the tube gel was transferred into a new 1.5 mL microtube.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1260233 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1260233
Funding provided by Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) Award Number: GBMF3782 Award URL: https://www.moore.org/grant-detail?grantId=GBMF3782
onGoing
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
asNeeded
Dataset Version: 5
Unknown
sample_id
MS_MS_sample_name
station_id
depth_m
latitude_dd
longitude_dd
date_y_m_d
time_h_m_s
ISO_DateTime_UTC
minimum_filter_size_microns
maximum_filter_size_microns
cruise_id
protein_id
spectral_count_sum
molecular_weight_kDa
protein_name
ncbi_id
ncbi_name
kegg_id
kegg_description
kegg_pathway
pfams_id
pfams_name
uniprot_id
enzyme_comm_id
5 kD membrane centrifuge
McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA)
theme
None, User defined
sample identification
station
depth
latitude
longitude
date_local
time_local
ISO_DateTime_UTC
filter_size
Cruise identfier
protein_ID
spectral counts
weight
sample description
taxon_code
verbatim identification
accession number
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Centrifuge
McLane Pump
instrument
BCO-DMO Standard Instruments
KM1128
service
Deployment Activity
tropical North Pacific along 150 degrees West from 18 degrees North to the equator
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03)
https://osprey.bco-dmo.org/project/2236
Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03)
<p>MetZyme project researchers will determine the role of enzymatic activity in the cycling of trace metals. Specifically the research will address the following questions: (1) degradation of sinking particulate organic material in the Tropical North Pacific can be influenced by the ability of microbes to synthesize zinc proteases, which in turn is controlled by the abundance or availability of zinc, and (2) methylation of mercury is controlled, in part, by the activity of cobalt-containing enzymes, and therefore the supply of labile cobalt to the corrinoid-containing enzymes or co-factors responsible for methylation. To attain their goal, they will collect dissolved and particulate samples for trace metals and metalloenzymes from three stations along a biogeochemical gradient in the Tropical North Pacific (along 150 degrees West from 18 degrees North to the equator). Sinking particles from metal clean sediment traps will also be obtained. The samples will also be used to carry out shipboard incubation experiments using amendments of metals, metal-chelators, B12, and proteases to examine the sensitivity and metal limitation of heterotrophic, enzymatic degradation of organic matter within the oceanic "Twilight Zone" (100-500 m). This study will result in a novel metaproteomic/metalloenzyme datasets that should provide insights into the biogeochemical cycling of metals, as well as co-limitation of primary productivity and controls on the export of carbon from the photic zone. In addition to the final data being contributed to BCO-DMO, an online metaproteomic data server will be created so the community has access to the raw data files generated by this research.</p>
MetZyme
largerWorkCitation
project
eng; USA
oceans
tropical North Pacific along 150 degrees West from 18 degrees North to the equator
153.2745
175.9503
-15.7878
20.6372
2011-10-01
2011-10-25
Tropical North Pacific along 150 degrees West from 18 degrees North to the equator
0
BCO-DMO catalogue of parameters from Total spectral count of proteins from R/V Kilo Moana cruise KM1128 for the METZYME expedition in the tropical North Pacific in 2011.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/983873.rdf
Name: sample_id
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/983874.rdf
Name: MS_MS_sample_name
Units: unitless
Description: <p>Unique name for the mass spec sample and run</p>
http://lod.bco-dmo.org/id/dataset-parameter/983875.rdf
Name: station_id
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/983876.rdf
Name: depth_m
Units: meters
Description: <p>Depth of sampling event</p>
http://lod.bco-dmo.org/id/dataset-parameter/983877.rdf
Name: latitude_dd
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/983878.rdf
Name: longitude_dd
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/983879.rdf
Name: date_y_m_d
Units: unitless
Description: <p>Date (local) of sampling at station and depth (HST, UTC-10)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983880.rdf
Name: time_h_m_s
Units: unitless
Description: <p>Time (local) of sampling at station and depth (HST, UTC-10)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983881.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Sampling datetime with timezone (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/983882.rdf
Name: minimum_filter_size_microns
Units: microns
Description: <p>Minimum filter size</p>
http://lod.bco-dmo.org/id/dataset-parameter/983883.rdf
Name: maximum_filter_size_microns
Units: microns
Description: <p>Maximum filter size</p>
http://lod.bco-dmo.org/id/dataset-parameter/983884.rdf
Name: cruise_id
Units: unitless
Description: <p>Cruise deployment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/983885.rdf
Name: protein_id
Units: unitless
Description: <p>The specific name of the full protein length sequence assembled in the metagenome that was used for peptide identification. An identifier that uniquely identifies this protein within this dataset and the FASTA file (see Related Datasets).</p>
http://lod.bco-dmo.org/id/dataset-parameter/983886.rdf
Name: spectral_count_sum
Units: count
Description: <p>Spectral count of proteins at each station and depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/983887.rdf
Name: molecular_weight_kDa
Units: kilodalton (kDa)
Description: <p>Molecular weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/983888.rdf
Name: protein_name
Units: unitless
Description: <p>Descriptive name of the function of the protein</p>
http://lod.bco-dmo.org/id/dataset-parameter/983889.rdf
Name: ncbi_id
Units: unitless
Description: <p>NCBI Taxonomy organism identifier (for ncbi_name)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983890.rdf
Name: ncbi_name
Units: unitless
Description: <p>NCBI Taxonomy name (corresponding to ncbi_id)</p>
http://lod.bco-dmo.org/id/dataset-parameter/983891.rdf
Name: kegg_id
Units: unitless
Description: <p>The Kegg Orthology Entry identifier for the best Kegg match.</p>
http://lod.bco-dmo.org/id/dataset-parameter/983892.rdf
Name: kegg_description
Units: unitless
Description: <p>Description of the function of the specific KEGG protein group</p>
http://lod.bco-dmo.org/id/dataset-parameter/983893.rdf
Name: kegg_pathway
Units: unitless
Description: <p>Decription of the cellular pathway that the KEGG protein is a part of.</p>
http://lod.bco-dmo.org/id/dataset-parameter/983894.rdf
Name: pfams_id
Units: unitless
Description: <p>Protein family (Pfam) ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/983895.rdf
Name: pfams_name
Units: unitless
Description: <p>Protein family (Pfam) description</p>
http://lod.bco-dmo.org/id/dataset-parameter/983896.rdf
Name: uniprot_id
Units: unitless
Description: <p>Uniprot database ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/983897.rdf
Name: enzyme_comm_id
Units: unitless
Description: <p>Enzyme Commission ID number</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/730722/data/download
download
onLine
dataset
<p>Ocean metaproteomes were sampled and extracted as described in Saito et al., 2014. The protocols are reproduced here:</p>
<p>Samples were collected during the KM1128 METZYME research expedition (Metals and Enzymes in the Pacific) on the R/V Kilo Moana October 1-25, 2011 from Oahu, Hawaii to Apia, Samoa, with Carl Lamborg and Mak Saito as Chief Scientists. Microbial biomass for protein analyses was collected on vertical profiles using in situ high volume particle filtration pumps with a focus on the North Pacific and Equatorial regions. Protein samples were collected by a suite of 4 L/min and 8 L/min McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA) outfitted with custom Mini-MULVS multiple filter head systems. The 0.2-3.0 micron size fraction was collected on 142 mm filters (Supor, Pall Corp.) for analyses used in this study. The volume of water was pumped until a minimal flow rate was achieved or the allotted cast time period expired, typically ~300-500 L. Filters were sectioned immediately after pump retrieval, and protein samples (¼ filter) were stored in RNAlater reagent (Ambion, Life Technologies), which has been shown to be an effective preservative for cyanobacterial biomass (Saito et al., 2011), frozen at -80C, transported back to the laboratory on dry ice, and stored at -80C until analysis.</p>
<p>Total microbial protein (0.2-3.0um fraction) was extracted using detergent-based methods, described below. Total protein showed enhanced concentrations in the photic zone, particularly in the Equatorial and South Pacific portions of the transect (Fig. S1F). For protein extraction, samples were thawed and the filter and RNAlater preservative (Ambion Life Technologies) were separated. The removed preservative was spin-concentrated by a 5K MWCO membrane (Sartorius Stedim Biotech 6 mL, 5 K MWCO Vivaspin units; Goettingen, Germany), and rinsed with 0.1M Tris buffer to recover, desalt, and concentrate any suspended material. The sample filter was unfolded and placed in a larger tube to which 1% SDS extraction buffer (1% SDS, 0.1M Tris/HCL pH 7.5, 10mM EDTA) and the rinsed/desalted RNAlater fraction was added back. Each sample was incubated at room temperature for 15 minutes, heated at 95C for 10 minutes, and shaken at room temperature for 350 rpm for 1 h. The protein extract was decanted and placed in a new tube and centrifuged for 30 min x 3220 g at room temperature. The supernatants were removed and filtered through a 5 um low protein binding syringe filter (Fisher Scientific), and the filter rinsed with extraction buffer. The extracts were concentrated by 5 kD membrane centrifugation to a small volume, washed with extraction buffer, and concentrated again. Each sample was precipitated with cold 50% methanol (MeOH) 50% acetone 0.5 mM HCL for 3 days at –20C, centrifuged at 14100 x g (14500 rpm) for 30 m at 4C, decanted and dried by vacuum concentration (Thermo Savant Speedvac) for 10 min or until dry. Pellets were resuspended in 1% SDS extraction buffer and left at room temperature (RT) for 1 h to redissolve. Total protein was quantified (Bio-Rad DC protein assay, Hercules, CA) with BSA as a standard.</p>
<p>Extracted proteins were purified from SDS detergent, reduced, alkylated, and&nbsp; trypsin digested, while embedded within a polyacrylamide tube gel, using a modified protocol from a previously published method (38). The tube gel approach allowed all proteins including membrane proteins to be solubilized by detergent and purified while immobilized in the gel matrix. A gel premix was made by combining 1 M Tris HCL (pH 7.5) and 40% Bis-acrylimide L 29:1 (Acros Organics) at a ratio of 1:3. The premix (103 ul) was combined with an extracted protein sample (usually 25 ug-200 ug), TE, 7 ul 1% APS and 3 ul of TEMED (Acros Organics) to a final volume of 200 ul. After 1 h of polymerization at RT, 200 ul of gel fix solution (50% ETOH, 10% acetic acid in LC/MS grade water) was added to the top of the gel and incubated at RT for 20 minutes. Liquid was then removed and the tube gel was transferred into a new 1.5 mL microtube.</p>
Specified by the Principal Investigator(s)
<p>&nbsp;</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
Data Version 5 (2022-06-06) replaces data version 4.
* This version updates the to match the data ingested into the Ocean Protein Portal as version 5 at OPP.
* Both the OPP and the BCO-DMO datasets page imported data from source file "METZYME_02_proteins_for_OPP.csv" file date 2022-06-06. These data will appear as file "730722_v5_metzyme_0_pt_2_proteins.csv" on the BCO-DMO dataset page.
* This version of the BCO-DMO dataset did not join with the related station information dataset (https://www.bco-dmo.org/dataset/730891 version 3) as was done with past versions since the collection information (lat,lon, date,time (local), cruise) was included in source file METZYME_02_peptides_for_OPP.csv.
* Additional datetime column added in ISO Format (utc time zone), coverted from local HST time provided.
Data Version 4 (2021-03-30) replaces data version 2 (2018-05-17). There was never a published version 3 of this dataset.
- Data version 4 uses updated station information (dataset 730891 version 3) joined with the submitted file Metzyme_Proteins_V1_targetdecoyPSMvalidator_030918.xlsx. Also several transformation and find and replace operations performed as described below.
- Sheet "Final" imported into the BCO-DMO data system from file Metzyme_Proteins_V1_targetdecoyPSMvalidator_030918.xlsx
#- N/A interpreted as no data value
- skipped row that started "END"
- Data unpivoted to transform data in the header into depth and station columns and put all spectral counts in one "spectral_count" column.
- Columns renamed to fit the OPP template (see https://github.com/oceanproteinportal/data-file-templates).
- Added minimum_filter_size_microns column with value '0.2'
- Added maximum_filter_size_microns with 'value 3.0'
- Added station information from dataset 730891 version 3 using a join operation station and depth as keys. - Added dates times (local and UTC), and lat, lon.
- removed prefixes and suffixes (e.g. \|\|_GAP_) and changed delimiter within a value to ; from || in kegg_id, kegg_descriptoin, pframs_id, and pframs_name, uniprot_id, enzyme_comm_id.
stripped whitespace around internal cell delimiter ;
- file saved as csv format.
BCO-DMO Processing Notes Data version 2 (2018-05-17):
- Added column KO_link for the protein links at NCBI
- Replaced blank cells with nd
- Replaced #N/A with NA
- Replaced commas in cells with ;
- Removed first column which contained the row number
- Added cruise column for relevant deployment information
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5 kD membrane centrifuge
5 kD membrane centrifuge
PI Supplied Instrument Name: 5 kD membrane centrifuge PI Supplied Instrument Description:Used to concentrate the extracts Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA)
McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA)
PI Supplied Instrument Name: McLane Pumps (WTS-LV; McLane Research Laboratories Inc., Falmouth MA, USA) PI Supplied Instrument Description:Used to collect microbial biomass for protein analyses Instrument Name: McLane Pump Instrument Short Name:McLane Pump Instrument Description: McLane pumps sample large volumes of seawater at depth. They are attached to a wire and lowered to different depths in the ocean. As the water is pumped through the filter, particles suspended in the ocean are collected on the filters. The pumps are then retrieved and the contents of the filters are analyzed in a lab.
Cruise: KM1128
KM1128
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1128
Carl Lamborg
Woods Hole Oceanographic Institution
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel