http://lod.bco-dmo.org/id/dataset/731187
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-03-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)
2018-03-26
publication
2018-03-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-03
publication
https://doi.org/10.1575/1912/bco-dmo.731187.1
Janet E. Kubler
California State University Northridge
principalInvestigator
Steve Dudgeon
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-26 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.731187.1 [access date]
Plocamium carbon and nitrogen content and stable isotope values Dataset Description: <p>This dataset includes carbon and nitrogen content and stable isotope values from <em>Plocamium cartilagineum</em> tissue grown under various temperatures and CO2 levels, from July 2014 to February 2015.</p>
<p><strong>Related Datasets:</strong><br />
<a href="https://www.bco-dmo.org/dataset/726664">Plocamium culture carbonate chemistry</a>: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731237">Plocamium culture: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731134">Plocamium cultures pH and temperature</a>: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731452">Plocamium exptl treatments summary</a>: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726452">Plocamium growth and biomass</a>: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731213">Plocamium pigments</a>: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726480">Plocamium: pH drift</a>: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/733238">Rapid Light Curves_PAM</a>: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions</p> Methods and Sampling: <p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Tissue samples were dried for 24 hours at 60°C. Dried samples were prepared for analysis by homogenizing samples using a metal laboratory scoop, cleaned with ethanol between each sample, which resulted in a fine power. Then, approximately 3 mg of the Plocamium cartilagineum tissue powder was measured, using an analytical balance, into a tin capsule and carefully enclosed with cleaned forceps. The tin capsules were put into 96-well trays and sent to the University of California, Davis Stable Isotope Facility (UCD-SIF). The samples were analyzed for delta-15N and delta-13C using the elemental analysis – isotope ratio mass spectrometry technique, which also provides results for tissue C and N content.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316198
completed
Janet E. Kubler
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
janet.kubler@csun.edu
pointOfContact
Steve Dudgeon
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
steve.dudgeon@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Sample_ID
d13C
C_mass_sample
pcent_C
d15N
N_mass_sample
pcent_N
C_N
mass_plant
Flag
Analytical balance (Mettler Toledo XP205)
theme
None, User defined
date_local
sample identification
d13C measured in biota
total organic Carbon
d15N measured in biota
total organic Nitrogen
Carbon to Nitrogen ratio (C:N)
mass_dry
flag
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
https://osprey.bco-dmo.org/project/2275
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
<p>Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, <em>Ulva</em> spp. and <em>Plocamium cartilagineum</em>, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in <em>Plocamium</em>, which relies solely on diffusive uptake of CO2, and populations of<em> Ulva</em> spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of<em> Ulva</em> spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on <em>Ulva</em> spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.</p>
Seaweed OA Resilience
largerWorkCitation
project
eng; USA
biota
oceans
-119
-118
33
34
2014-02-23
2015-02-23
Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
0
BCO-DMO catalogue of parameters from Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/731201.rdf
Name: Date
Units: unitless
Description: <p>Sample collection date formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/731202.rdf
Name: Sample_ID
Units: unitless
Description: <p>Trial number: time=0 or end (E); pot number; replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/731203.rdf
Name: d13C
Units: ppt
Description: <p>Isotopic composition of delta 13C of plant relative to Pee Dee Belemnite (PDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/731204.rdf
Name: C_mass_sample
Units: micrograms
Description: <p>Mass of carbon in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/731205.rdf
Name: pcent_C
Units: unitless
Description: <p>Percentage of total mass that is carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/731206.rdf
Name: d15N
Units: ppt
Description: <p>isotopic composition of delta 15N of plant relative to air</p>
http://lod.bco-dmo.org/id/dataset-parameter/731207.rdf
Name: N_mass_sample
Units: micrograms
Description: <p>Mass of nitrogen in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/731208.rdf
Name: pcent_N
Units: unitless
Description: <p>Percentage of total mass that is Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/731209.rdf
Name: C_N
Units: unitless
Description: <p>Ratio (by mass) of Carbon to Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/731210.rdf
Name: mass_plant
Units: milligrams
Description: <p>Mass of plant tissue sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/731211.rdf
Name: Flag
Units: unitless
Description: <p>Quality flag: y means UCD Stable Isotope facility flagged as imprecise due to low quantity of C or N</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5970
https://darchive.mblwhoilibrary.org/bitstream/1912/24190/1/dataset-731187_plocamium-chn-and-stable-isotopes__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.731187.1
download
onLine
dataset
<p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Tissue samples were dried for 24 hours at 60°C. Dried samples were prepared for analysis by homogenizing samples using a metal laboratory scoop, cleaned with ethanol between each sample, which resulted in a fine power. Then, approximately 3 mg of the Plocamium cartilagineum tissue powder was measured, using an analytical balance, into a tin capsule and carefully enclosed with cleaned forceps. The tin capsules were put into 96-well trays and sent to the University of California, Davis Stable Isotope Facility (UCD-SIF). The samples were analyzed for delta-15N and delta-13C using the elemental analysis – isotope ratio mass spectrometry technique, which also provides results for tissue C and N content.</p>
<p>Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.</p>
<p>See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- removed trailing hyphen after date<br />
- replaced blank cells with 'nd', no data<br />
- sorted records by date</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Instrument located at the University of California, Davis Stable Isotope Facility (UCD-SIF) and used to measure isotope carbon and nitrogen isotopes. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Analytical balance (Mettler Toledo XP205)
Analytical balance (Mettler Toledo XP205)
PI Supplied Instrument Name: Analytical balance (Mettler Toledo XP205) PI Supplied Instrument Description:Used to weigh dried plant tissue samples. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/