<div><p><em>Plocamium </em><em>cartilagineum</em> was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.</p>
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 micro-moles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses. In each culture pot, pCO2 was set by the supply rate of CO2 in the corresponding mass-flow controlled gas mixing system to be within a target range of either near ambient, moderately elevated or highly elevated in each trial. The near-ambient range was narrower than the other target ranges (set points typically ~380 - 390 µatm) as it served as the control range in each trial. However, actual pCO2 in solution in each culture pot varied slightly from constant target values on a diurnal cycle associated with the metabolic activities of the algae contained within each pot. Unique average values of pCO2 in each culture pot based on different set values for each mass-flow controlled mixer within the qualitative ranges of ambient, moderate and highly increased pCO2 levels that were replicated in each trial of the experiment enabled a more powerful regression-type experimental design. With a regression type approach, we could estimate the functional relationship between response variables and pCO2, which was not possible with a simple categorical treatment design.</p>
<p>See Supplementary File for a table of culture conditions for each of the 8 trials (pdf).</p></div>
Plocamium experimental treatments summary
<div><p>This dataset includes a summary of the experimental pCO2 and temperature treatments for <em>Plocamium cartilagineum</em> cultures.</p>
<p><strong>Related Datasets:</strong><br /><a href="https://www.bco-dmo.org/dataset/731187">Plocamium carbon nitrogen and stable isotopes</a>: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726664">Plocamium culture carbonate chemistry</a>: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731237">Plocamium culture: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731134">Plocamium cultures pH and temperature</a>: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726452">Plocamium growth and biomass</a>: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/731213">Plocamium pigments</a>: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/726480">Plocamium: pH drift</a>: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)</p>
<p><a href="https://www.bco-dmo.org/dataset/733238">Rapid Light Curves_PAM</a>: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions</p></div>
Plocamium exptl treatments summary
<div><p><strong>BCO-DMO Processing Notes:</strong><br />
- added a conventional header with dataset name and description, PI names, version date</p></div>
731452
Plocamium exptl treatments summary
2018-03-23T10:13:38-04:00
2018-03-23T10:13:38-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:731452
Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)
Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial.
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Kubler, J. E., Dudgeon, S. (2018) Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/731452 [access date]
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