http://lod.bco-dmo.org/id/dataset/732091 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2018-03-27 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Wirewalker data from the EAGER DNA BioPump project publication revision BCO-DMO Linked Data URI creation http://lod.bco-dmo.org/id/dataset/732091 Melissa Omand University of Rhode Island principalInvestigator Colleen Durkin Moss Landing Marine Laboratories principalInvestigator Margaret L. Estapa Skidmore College principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Omand, M., Estapa, M. L., Durkin, C. (1969) Wirewalker data. Biological and Chemical Oceanography Data Management Office (BCO-DMO). [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/732091 [access date] Dataset Description: &lt;p&gt;This dataset includes data from Wirewalker deployments during the R/V Falkor cruise&amp;nbsp;FK170124 as part of the&amp;nbsp;Sea to Space Particle Investigation project (see &lt;a href=&quot;https://schmidtocean.org/cruises/&quot; target=&quot;_blank&quot;&gt;https://schmidtocean.org/cruises/&lt;/a&gt;).&lt;/p&gt; &lt;p&gt;These data were utilized in the following publication (Omand et al., 2017).&lt;br /&gt; Methods and Sampling: &lt;p&gt;The data was acquired from a Wirewalker platform. The Wirewalker uses wave energy to propel an instrument package vertically along a wire suspended from a buoy at the sea surface. A more detailed description of the Wirewalker can be found in Rainville and PInkel, (2001) “Wirewalker: An Autonomous Wave-Powered Vertical Profiler”. The average descent rate of the Wirewalker was about 0.4 m/s and the ascent was 0.7 m/s, quickly reaching a terminal velocity determined by a combination of buoyancy and drag. The time required to make a complete round-trip to 120 m varied from 7-10 minutes. Temperature, salinity, and depth were measured with a Maestro CTD system (Richard Branker Research), and an integrated WET Labs Ecotriplet measured chlorophyll a fluorescence (FL), chromophoric dissolved organic matter (CDOM), and backscatter at λ = 700 nm. Downwelling solar irradiance was measured with a JFE Advantech cosine miniature photo- synthetically active radiation (PAR) sensor. Beam attenuation was measured with a WET Labs C-Star (λ = 650 nm) mounted vertically with brackets that allowed a minimally interrupted flow past the sensing volume during ascent. The Wirewalker also had a Rinko dissolved oxygen sensor, but the sensor appears to have gotten bleached and was not useable. The CTD sampled a 6 Hz. The bio-optical and PAR sensors sampled at 1 Hz. Individual profiles were isolated and the downcast data was discarded. The upcast data was interpolated onto 2m vertical bins.&amp;nbsp;&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1703336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1703336 onGoing Melissa Omand University of Rhode Island 401-874-6610 215 S Ferry Rd Narrangsett RI 02882-1197 USA momand@uri.edu pointOfContact Colleen Durkin Moss Landing Marine Laboratories 831-771-4431 8272 Moss Landing Road Moss Landing CA 95039-9647 USA cdurkin@mbari.org pointOfContact Margaret L. Estapa Skidmore College 207-581-5176 193 Clarks Cove Road Walpole ME 04573 United States margaret.estapa@maine.edu pointOfContact asNeeded Unknown FK170124 service Deployment Activity North Pacific Ocean place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: EAGER: Particle-specific DNA sequencing to directly observe ecological mechanisms of the biological pump https://osprey.bco-dmo.org/project/675296 Collaborative Research: EAGER: Particle-specific DNA sequencing to directly observe ecological mechanisms of the biological pump <p><em>NSF Award Abstract:</em><br /> Carbon is fixed into organic matter by phytoplankton growing in the surface ocean, and is naturally sequestered in the ocean interior when particles and organisms sink: a process called the "biological pump." Because of its recognized influence on the global carbon cycle, ocean scientists have studied the biological pump for decades. However, we still do not have a sufficient understanding of the underlying processes to accurately quantify and predict carbon cycling. Much of this uncertainty stems from an inability to directly link specific plankton in the surface ocean with the types of particles sinking out of the surface ocean. To address this missing link in biological pump research, this work will directly observe how plankton are transported out of the surface ocean using novel, particle-specific observational approaches embedded within an interdisciplinary field program that will finely resolve upper ocean plankton groups and the resulting amount of sinking carbon across space and in time. The genetic identity of organisms within different types of sinking particles will be determined by sequencing the genetic contents of individually collected particles. This new application of a molecular method will definitively link surface plankton with sinking particles at five locations across the Pacific Ocean. This work has the potential to transform our understanding of the biological pump by identifying previously unknown links between surface ecosystems and sinking carbon particles. Because this work is embedded within an interdisciplinary field program, including biogeochemical modelers and remote sensing scientists, these data will feed directly into new models of the biological pump, improving our ability to quantify and predict carbon uptake by the ocean. This project will train 1 graduate student and at least 2 undergraduate researchers. Findings will be communicated to the non-scientific public through blogs, videos, and the public communication channels of participating institutions.</p> <p>Accurate prediction of the global carbon cycle requires an understanding of the specific processes that link surface plankton communities and sinking particulate carbon flux (export) out of the surface ocean, but current methodological paradigms in biological pump research do not directly observe these processes. This project will comprehensively determine who is exported from the surface ocean and how using new, particle-resolving optical and molecular techniques embedded within a sampling scheme that characterizes export events at high time and space resolution. The investigation suggests that different plankton types in the surface waters are transported out of the surface ocean by distinct export pathways, and that an understanding of these connections is critical knowledge for global carbon cycle modeling. If successful, this work has the potential to transform our conceptual understanding of the biological pump by directly identifying mechanisms that link surface plankton with particle export, without relying on bulk sampling schemes and large-scale correlation analysis. Particle export environments will be studied at five open ocean locations during a cruise from Hawaii to Seattle in January-February 2017. The surface plankton communities will be characterized by a combination of satellite observations, sensors attached to a free-drifting, continuously profiling WireWalker, an in situ holographic camera, microscopy, and by sequencing 18S and 16S rRNA gene fragments. Exported particles will simultaneously be captured by various specialized sediment traps and their characteristics will be directly related to their sources in the surface community by identifying the genetic contents of individual particle types. Individual particles will be isolated from gel layers and the 16S and 18S rRNA gene fragments will be amplified and sequenced. This work would, for the first time, combine molecular approaches with particle-specific observations to enable simultaneous identification of both which organisms are exported and the processes responsible for their export.</p> EAGER DNA BioPump largerWorkCitation project eng; USA oceans North Pacific Ocean Eastern Pacific 0 BCO-DMO catalogue of parameters from Wirewalker data from the EAGER DNA BioPump project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/732091/data/download download onLine dataset &lt;p&gt;The data was acquired from a Wirewalker platform. The Wirewalker uses wave energy to propel an instrument package vertically along a wire suspended from a buoy at the sea surface. A more detailed description of the Wirewalker can be found in Rainville and PInkel, (2001) “Wirewalker: An Autonomous Wave-Powered Vertical Profiler”. The average descent rate of the Wirewalker was about 0.4 m/s and the ascent was 0.7 m/s, quickly reaching a terminal velocity determined by a combination of buoyancy and drag. The time required to make a complete round-trip to 120 m varied from 7-10 minutes. Temperature, salinity, and depth were measured with a Maestro CTD system (Richard Branker Research), and an integrated WET Labs Ecotriplet measured chlorophyll a fluorescence (FL), chromophoric dissolved organic matter (CDOM), and backscatter at λ = 700 nm. Downwelling solar irradiance was measured with a JFE Advantech cosine miniature photo- synthetically active radiation (PAR) sensor. Beam attenuation was measured with a WET Labs C-Star (λ = 650 nm) mounted vertically with brackets that allowed a minimally interrupted flow past the sensing volume during ascent. The Wirewalker also had a Rinko dissolved oxygen sensor, but the sensor appears to have gotten bleached and was not useable. The CTD sampled a 6 Hz. The bio-optical and PAR sensors sampled at 1 Hz. Individual profiles were isolated and the downcast data was discarded. The upcast data was interpolated onto 2m vertical bins.&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;RBR Ruskin software was used to download data and export as a text file.&lt;/p&gt; &lt;p&gt;MatLab was used for all subsequent data processing.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;QUALITY CONTROL STEPS:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Minimal QC was required for this dataset. The raw temp, sal, PAR were in good shape. The bio-optics required minor adjustment as described below.&lt;/p&gt; &lt;p&gt;FL contained spurious negative values (0.1% of the total). These were modified with a criteria that set values &amp;lt; 50 (lowest dark counts) to 50.&lt;/p&gt; &lt;p&gt;bbp contained spurious negative values (0.08% of the total). These were modified with a criteria that set values &amp;lt; 50 (lowest dark counts) to 50.&lt;/p&gt; &lt;p&gt;CDOM contained spurious high points (0.18% of the total) and negative values (3.2% of the total). These were modified with a criteria that set values &amp;lt; 41 (lowest dark counts) to 41, and values &amp;gt; 55 to 44 (median value).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;CORRECTION FOR NON-PHOTOCHEMICAL QUENCHING&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;The FL data show a clear diel pattern in the upper 30 m, correlated with PAR. This quenching during high light is attributed to NPQ of fluorescence. Therefore as a first steps toward getting values useful for calibration to mgChl/m^3, the data was corrected for this signal. The correction was obtained from a linear regression between the PAR and raw FL values at each depth bin. These regressions show a relatively linear relationship over the course of each 24 hour period, with the highest FL observed at night and quenching during higher light conditions. The daytime quenching is adjusted based on the slope and intercept at each depth to approximately match the night-time values.&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Cruise: FK170124 FK170124 R/V Falkor Community Standard Description International Council for the Exploration of the Sea R/V Falkor vessel FK170124 Ivona Cetinic National Aeronautics and Space Administration https://datadocs.bco-dmo.org/docs/302/EAGER_DNA_BioPump/data_docs/DurkinOmandEstapa_Cruise_report.pdf Report describing FK170124 R/V Falkor Community Standard Description International Council for the Exploration of the Sea R/V Falkor vessel