http://lod.bco-dmo.org/id/dataset/732438
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-03-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
2018-03-22
publication
2018-03-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-05
publication
https://doi.org/10.1575/1912/bco-dmo.732438.1
Janet E. Kubler
California State University Northridge
principalInvestigator
Steve Dudgeon
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-22 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.732438.1 [access date]
Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions Dataset Description: <p>This dataset reports the chlorophyll a concentration per unit biomass of Ulva lactuca&nbsp;grown in closed culture pots at varying pCO2 levels.</p>
<p><strong>Related Datasets:</strong><br />
<a href="https://www.bco-dmo.org/dataset/732177">Ulva: Carbonate chemistry pCO2</a>: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732564">Ulva: CHN and stable isotopes</a>: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732600">Ulva: Growth</a>: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732493">Ulva: pH and temperature time-series</a>: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732464">Ulva: pH Drift</a>: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732625">Ulva: Photosynthesis and respiration</a>: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732587">Ulva: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)</p> Methods and Sampling: <p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining nitrogen, oxygen and carbon dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being nitrogen.</p>
<p>Chlorophyll a was extracted from Ulva tissue following the procedure described in Duncan and Harrison (1982). Chlorophyll a concentrations were measured using a Shimadzu UV-2450 UV-visible spectrophotometer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316198
completed
Janet E. Kubler
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
janet.kubler@csun.edu
pointOfContact
Steve Dudgeon
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
steve.dudgeon@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Label
pCO2_avg
pCO2_sd
Fresh_Wt
DMSO_A665
Methanol_A668
Methanol_A635
DMSO_ext
Meth_ext
Total_chl_mg
chl_a
Shimadzu UV-2450 UV-visible spectrophotometer
theme
None, User defined
sample identification
Partial pressure of CO2
weight
absorbance
chlorophyll a
chl_tot
featureType
BCO-DMO Standard Parameters
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
https://osprey.bco-dmo.org/project/2275
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
<p>Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, <em>Ulva</em> spp. and <em>Plocamium cartilagineum</em>, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in <em>Plocamium</em>, which relies solely on diffusive uptake of CO2, and populations of<em> Ulva</em> spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of<em> Ulva</em> spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on <em>Ulva</em> spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.</p>
Seaweed OA Resilience
largerWorkCitation
project
eng; USA
biota
oceans
-119
-118
33
34
2015-05-11
2015-07-27
Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
0
BCO-DMO catalogue of parameters from Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/732453.rdf
Name: Label
Units: unitless
Description: <p>Trial number-pot number.frozen tissue replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/732454.rdf
Name: pCO2_avg
Units: microatmospheres (µatm)
Description: <p>Average pCO2 partial pressure in seawater tanks</p>
http://lod.bco-dmo.org/id/dataset-parameter/732455.rdf
Name: pCO2_sd
Units: microatmospheres (µatm)
Description: <p>Variability of pCO2 partial pressure - standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/732456.rdf
Name: Fresh_Wt
Units: grams
Description: <p>Fresh weight of tissue sample prior to deep freeze</p>
http://lod.bco-dmo.org/id/dataset-parameter/732457.rdf
Name: DMSO_A665
Units: unitless
Description: <p>Absorbance of light by ground Ulva tissue at 665nm in 1.25 ml of an 80% DMSO solution in water</p>
http://lod.bco-dmo.org/id/dataset-parameter/732458.rdf
Name: Methanol_A668
Units: unitless
Description: <p>Absorbance of light by ground Ulva tissue at 668nm in 6 ml methanol</p>
http://lod.bco-dmo.org/id/dataset-parameter/732459.rdf
Name: Methanol_A635
Units: unitless
Description: <p>Absorbance of light by ground Ulva tissue at 635nm in 6 ml methanol</p>
http://lod.bco-dmo.org/id/dataset-parameter/732460.rdf
Name: DMSO_ext
Units: milligram/liter (mg/L)
Description: <p>Chlorophyll a extracted in DMSO</p>
http://lod.bco-dmo.org/id/dataset-parameter/732461.rdf
Name: Meth_ext
Units: milligram/liter (mg/L)
Description: <p>Chlorophyll a in pooled extracts of 12 ml of methanol</p>
http://lod.bco-dmo.org/id/dataset-parameter/732462.rdf
Name: Total_chl_mg
Units: milligrams (mg)
Description: <p>Total chlorophyll a extracted from ground tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/732463.rdf
Name: chl_a
Units: milligrams/gram (mg/g)
Description: <p>Chlorophyll a concentration per unit biomass</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6205
https://darchive.mblwhoilibrary.org/bitstream/1912/24201/1/dataset-732438_ulva-chl__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.732438.1
download
onLine
dataset
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining nitrogen, oxygen and carbon dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being nitrogen.</p>
<p>Chlorophyll a was extracted from Ulva tissue following the procedure described in Duncan and Harrison (1982). Chlorophyll a concentrations were measured using a Shimadzu UV-2450 UV-visible spectrophotometer.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added a conventional header with dataset&nbsp;name and description, PI names, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Shimadzu UV-2450 UV-visible spectrophotometer
Shimadzu UV-2450 UV-visible spectrophotometer
PI Supplied Instrument Name: Shimadzu UV-2450 UV-visible spectrophotometer PI Supplied Instrument Description:Used to measure chlorophyll a concentration. Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/