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Samples were collected at 0 (initial) and 24 hours and parameters of carbonate chemistry were measured (DIC,CO2, HCO3,CO3-described below). pH drift assay experiments consisted of 2 independent variables; inhibitor treatment and algal presence, with 4 and 2 levels, respectively for each independent variable. The inhibitor treatments included: seawater only (control), Acetazolamide (A), 4,4\u2019-Diisothiocyano-2,2\u2019-stilbenedisulfonic acid (DIDS), and Ethoxyzolamide (E). Algae were either present or absent (control) in a vial. Two replicate water samples were collected from each of 10 experimental culture pots of algae grown for 3 weeks at different combinations of average and standard deviations of pCO2 level during each of 3 experimental trials and assayed after 24 hours with or without the presence of Ulva and combinations of inhibitor treatments. In addition, 3 different levels of pCO2 in seawater generated independently of the seawater in algal culture that represented the range of pCO2 levels assayed across the entire experiment were assayed over the same 24 hour period.<\/p>\n
Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Carbonate Chemistry over a time course with Ulva in pH drift experiments","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Seawater samples were collected from a pH drift experiment with Ulva lactuca to test the mechanism of carbon acquisition by the alga and the effect on carbonate chemistry parameters reported in these datasets. This dataset reports the pH, dissolved inorganic carbon (DIC), carbon diaoxide (CO2), bicarbonate and carbonate concentrations of seawater at initial and 24 hour times from pH drift experiment that used Ulva lactuca grown in closed culture pots at varying pCO2 levels.<\/p>\n
Related Datasets:<\/strong>
Ulva: Carbonate chemistry pCO2<\/a>: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: Chl a<\/a>: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Ulva: CHN and stable isotopes<\/a>: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Ulva: Growth<\/a>: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: pH and temperature time-series<\/a>: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: Photosynthesis and respiration<\/a>: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: seawater delta13C<\/a>: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Ulva: pH Drift","@type":"xsd:string"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"732464","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Ulva: pH Drift"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2018-03-28T08:53:51-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2018-03-28T08:53:51-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-09-06T15:20:05-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:732464","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/732464"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier732464"}],"http:\/\/ocean-data.org\/schema\/hasDataManagementProcessing":[{"@value":"- added a conventional header with dataset name and description, PI names, version date\r\n- modified parameter names to conform with BCO-DMO naming conventions","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"Carbonate chemistry measurements over a time course with Ulva in pH drift experiments.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent732464"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage732464"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Kubler, J., Dudgeon, S. 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