http://lod.bco-dmo.org/id/dataset/732818
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-04-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments
2018-04-05
publication
2018-04-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2018-07-24
publication
https://doi.org/10.1575/1912/bco-dmo.742200
Hannes Baumann
University of Connecticut
principalInvestigator
Christopher S. Murray
University of Connecticut
principalInvestigator
Janet Nye
Stony Brook University - SoMAS
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Murray, C., Baumann, H. (2018) CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 05 April 2018) Version Date 2018-04-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.742200 [access date]
CO2 x temperature specific early life survival and growth assessed by 5 factorial experiments Dataset Description: <p>CO2 x temperature specific early life survival and growth assessed by 5 factorial experiments.</p>
<p>These data are published in: Murray, C.S. &amp; Baumann, H. 2018 You better repeat it: complex temperature × CO2 effects in Atlantic silverside offspring revealed by serial experimentation. Diversity 10, 1-19. doi:<a href="http://www.dx.doi.org/10.3390/d10030069" target="_blank">10.3390/d10030069</a></p> Methods and Sampling: <p><strong>CO2 × temperature manipulations and measurements:</strong><br />
For 2 × 2 and 3 × 2 factorial designs, replicate rearing containers (20 L) were placed into large temperature-controlled water baths. Elevated CO<sub>2</sub> levels were achieved via gas proportioners (ColeParmer®) mixing air with 100% CO<sub>2 </sub>(bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone. To counteract metabolic CO<sub>2</sub> accumulation, control CO<sub>2</sub> conditions were achieved by forcing compressed laboratory air through a series of CO<sub>2</sub> stripping units containing granular soda lime (AirGas<sup>®</sup>), a particle filter (1 µm), and then to each replicate via airstone. Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach® HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pH<sub>NBS</sub> references. Continuous bubbling maintained dissolved oxygen saturation (&gt;8 mg/ DO) in rearing vessels. Target treatment temperatures were controlled by thermostats (Aqualogic<sup>®</sup>) which powered chillers (DeltaStar®) or glass submersible heaters to maintain water bath temperatures. For 3 × 3 factorial experiments, we developed an automated acidification system composed of nine discrete recirculation units designed for larval fish rearing. We designed a LabView (National Instruments<sup>®</sup>) based program to fully automate the control of seawater chemistry. The software interfaces with the recirculating units via a data-acquisition module (NI cDAQ-9184, National Instruments<sup>®</sup>), which controls nine sampling pumps (one per tank) and a series of gas and water solenoid valves, while receiving input from a central pH electrode (Hach pHD<sup>®</sup> digital electrode calibrated weekly using 2-point pH<sub>NBS</sub> references) and dissolved oxygen probe (Hach LDO<sup>®</sup> Model 2). The software sequentially assesses the pH conditions in each rearing unit (each tank once per hour) by pumping water for ~450 seconds through the housing of the central pH probe, comparing measured pH levels to set-points and then adjusting levels by bubbling standardized amounts 100% CO<sub>2</sub> (bone dry grade, AirGas<sup>®</sup>) or CO<sub>2</sub>-stripped air into the sump of each tank. The software also maintains DO saturation (&gt;8 mg/l) by bubbling in CO<sub>2</sub>-stripped air. LabView logs current pH, temperature, and DO conditions before cycling to the next unit. Temperatures were controlled by thermostats (Aqualogic<sup>®</sup>) that powered submersible heaters or in-line chillers (DeltaStar<sup>®</sup>).</p>
<p>Actual treatment CO<sub>2</sub> levels were determined based on measurements of pH, temperature, salinity, and total alkalinity (<em>A</em><sub>T</sub>). Treatment tanks were sampled three times per experiment for measurements of <em>A</em><sub>T</sub> (μmol kg<sup>-1</sup>). Seawater was siphoned and filtered (to 10 µm) into 300 ml borosilicate bottles. Salinity was measured at the time of sampling using a refractometer. Bottles were stored at 3˚C and measured for <em>A</em><sub>T</sub> within two weeks of sampling using an endpoint titration (Mettler Toledo<sup>®</sup>&nbsp;G20 Potentiometric Titrator). Methodological accuracy (within ±1%) of alkalinity titrations were verified and calibrated using Dr. Andrew Dickson’s (University of California San Diego, Scripps Institution of Oceanography) certified reference material for <em>A</em><sub>T</sub> in seawater. The partial pressure of CO<sub>2</sub> (pCO<sub>2,</sub>; μatm) was calculated in CO2SYS (V2.1, <a href="http://cdiac.ornl.gov/ftp/co2sys">http://cdiac.ornl.gov/ftp/co2sys</a>) based on measured <em>A</em><sub>T</sub>, pH<sub>NBS</sub>, temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973)&nbsp; refit by Dickson and Millero (1987)&nbsp; and Dickson (1990) for KHSO<sub>4</sub>.</p>
<p><strong>Field sampling and experimental designs:</strong><br />
Collections of wild, spawning ripe Atlantic silversides were made during high tide 1-3 days prior to full or new moons during the species spawning season. Adults were caught with a 30 m × 2 m beach seine from local salt marshes and transported live to our laboratory facilities. Ripe adults were held overnight at 20°C in well aerated tanks at low densities with no food and strip spawned the next day.</p>
<p>For each experiment, eggs from 20+ running-ripe females were gently mixed into shallow plastic dishes lined with 1 mm plastic window screening. 20+ males were stripped-spawned together into 500 ml glass beakers, mixed with seawater, stirred, then gently poured into spawning dishes and mixed with eggs for ~15 minutes. Screens were rinsed with seawater to remove unfertilized eggs and then soaked in a 100 ppm buffered iodine (Ovadine<sup>®</sup>) solution for 15 minutes to prevent fungal infection. Experiments were initiated within two hours of fertilization when replicate rearing vessels received precisely 100 embryos. Vessels were filled with clean seawater (filtered to 1 µm and UV sterilized). Optimal salinity (27-31) and light conditions (15 h light:9 h dark) for rearing <em>M. menidia </em>were maintained across experiments. Upon hatching larvae were immediately provided <em>ad libitum</em> rations of newly hatched brine shrimp nauplii (<em>Artemia salina, </em>San Francisco strain, brineshrimpdirect.com) and equal rations of powdered weaning diet (Otohime Marine Fish Diet, size A1, Reed Mariculture®). To quantify hatching survival, one day post first hatch larvae were counted by gently scooping small groups into replacement rearing vessels. For initial hatch measurements, random sub-samples (N = 10) from each replicate were preserved in 5% formaldehyde/freshwater solution buffered with saturated sodium tetraborate. All experiments were terminated when larvae reached ~10 mm standard length (SL). At termination, all survivors were counted and measured for standard length (SL, nearest 0.01 mm) via calibrated digital images (Image Pro Premier<sup>®</sup> V9.0).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536336
completed
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Christopher S. Murray
University of Connecticut
christopher.murray@whoi.edu
pointOfContact
Janet Nye
Stony Brook University - SoMAS
252-726-6841 ext 158
Institute of Marine Sciences 3431 Arendell St.
Morehead City
NC
28557
USA
jnye@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 05 April 2018
Unknown
experiment
species
adult_collection_site
latitude
longitude
tank
replicate
temperature
pH
pCO2
fertilization_date
hatch_sample_date
hatch_sample_age
final_sample_date
final_sample_age
rel_embryo_survival
rel_larval_survival
hatch_length
final_length
growth_rate
airstone
DeltaStar chiller
glass submersible heaters
dissolved oxygen probe
handheld pH probe
refractometer
beach seine
Mettler Toledo G20 Potentiometric Titrator
theme
None, User defined
experiment id
species scientific name (binomial)
site
latitude
longitude
tank
replicate
water temperature at measurement depth
pH
Partial pressure of CO2
date
age
No BCO-DMO term
length
growth
featureType
BCO-DMO Standard Parameters
Airstone
Aquarium chiller
Immersion heater
Oxygen Sensor
pH Sensor
Refractometer
Seine Net
Titrator
instrument
BCO-DMO Standard Instruments
Mumford_Cove_Subsurface_Buoy
Poquot_Beach
service
Deployment Activity
Mumford Cove, CT USA
Poquot Beach, NY USA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
https://osprey.bco-dmo.org/project/650184
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
<p><em>Description from NSF award abstract:</em><br />
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.</p>
<p>This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.</p>
<p><strong>Related references:</strong><br />
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:<a href="http://dx.doi.org/10.1098/rsbl.2015.0976" target="_blank">10.1098/rsbl.2015.0976</a></p>
<p>Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:<a href="http://dx.doi.org/10.1002/loe2.10002" target="_blank">10.1002/loe2.10002</a></p>
<p>Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:<a href="http://dx.doi.org/10.3354/meps11142" target="_blank">10.3354/meps11142</a></p>
HYPOA
largerWorkCitation
project
eng; USA
biota
oceans
Mumford Cove, CT USA; Poquot Beach, NY USA
-73.10258
-72.015247
40.947376
41.321526
2014-05-05
2017-06-01
Eastern Long Island Sound, CT, USA
0
BCO-DMO catalogue of parameters from CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experiments
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/732836.rdf
Name: experiment
Units: unitless
Description: <p>Experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/732837.rdf
Name: species
Units: unitless
Description: <p>Scientific name of the speices (Menidia menidia, the Atlantic silverside)</p>
http://lod.bco-dmo.org/id/dataset-parameter/732838.rdf
Name: adult_collection_site
Units: unitless
Description: <p>Site of the collection of wild adult spawners</p>
http://lod.bco-dmo.org/id/dataset-parameter/732839.rdf
Name: latitude
Units: decimal degrees
Description: <p>Latitude of adult collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/732840.rdf
Name: longitude
Units: decimal degrees
Description: <p>Longitude of adult collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/732841.rdf
Name: tank
Units: unitless
Description: <p>Tank number</p>
http://lod.bco-dmo.org/id/dataset-parameter/732842.rdf
Name: replicate
Units: unitless
Description: <p>Within experiment replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/732843.rdf
Name: temperature
Units: degrees Celsius
Description: <p>Average temperature experienced by fish in degrees celsius</p>
http://lod.bco-dmo.org/id/dataset-parameter/732844.rdf
Name: pH
Units: pH NBS
Description: <p>Average pH level experienced by the fish NBS</p>
http://lod.bco-dmo.org/id/dataset-parameter/732845.rdf
Name: pCO2
Units: atm
Description: <p>Calculated average pCO2 levels in µatm calculated by CO2SYS based on alkalinity, pH, and temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/732846.rdf
Name: fertilization_date
Units: unitless
Description: <p>Date offspring were fertilized by stripspawning 20+ adults of each sex; formatted as yyyy/mm/dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/732847.rdf
Name: hatch_sample_date
Units: unitless
Description: <p>Date offspring were sampled for hatch survival and measurements; formatted as yyyy/mm/dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/732848.rdf
Name: hatch_sample_age
Units: days
Description: <p>Age in days post fertilization (dpf) on the date of hatch sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/732849.rdf
Name: final_sample_date
Units: unitless
Description: <p>Date offspring were sampled for final survival and measurements; formatted as yyyy/mm/dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/732850.rdf
Name: final_sample_age
Units: days
Description: <p>Age in days post fertilization (dpf) on the date of final sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/732851.rdf
Name: rel_embryo_survival
Units: unitless (fraction)
Description: <p>relative survival of embryos (0-1)</p>
http://lod.bco-dmo.org/id/dataset-parameter/732852.rdf
Name: rel_larval_survival
Units: unitless (fraction)
Description: <p>relative survival from hatch to experiment termination (0-1)</p>
http://lod.bco-dmo.org/id/dataset-parameter/732853.rdf
Name: hatch_length
Units: millimeters (mm)
Description: <p>Average replicate hatch length (mm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/732854.rdf
Name: final_length
Units: millimeters (mm)
Description: <p>Average replicate final length (mm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/732855.rdf
Name: growth_rate
Units: millimeters per day (mm d-1)
Description: <p>average replicate growth rate (mm d-1) of offspring from hatch to experiment termination</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
19127
https://darchive.mblwhoilibrary.org/bitstream/1912/10489/1/data_co2-x-temperature-effects-menidia-menidia-offspring.tsv
download
https://doi.org/10.1575/1912/bco-dmo.742200
download
onLine
dataset
<p><strong>CO2 × temperature manipulations and measurements:</strong><br />
For 2 × 2 and 3 × 2 factorial designs, replicate rearing containers (20 L) were placed into large temperature-controlled water baths. Elevated CO<sub>2</sub> levels were achieved via gas proportioners (ColeParmer®) mixing air with 100% CO<sub>2 </sub>(bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone. To counteract metabolic CO<sub>2</sub> accumulation, control CO<sub>2</sub> conditions were achieved by forcing compressed laboratory air through a series of CO<sub>2</sub> stripping units containing granular soda lime (AirGas<sup>®</sup>), a particle filter (1 µm), and then to each replicate via airstone. Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach® HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pH<sub>NBS</sub> references. Continuous bubbling maintained dissolved oxygen saturation (&gt;8 mg/ DO) in rearing vessels. Target treatment temperatures were controlled by thermostats (Aqualogic<sup>®</sup>) which powered chillers (DeltaStar®) or glass submersible heaters to maintain water bath temperatures. For 3 × 3 factorial experiments, we developed an automated acidification system composed of nine discrete recirculation units designed for larval fish rearing. We designed a LabView (National Instruments<sup>®</sup>) based program to fully automate the control of seawater chemistry. The software interfaces with the recirculating units via a data-acquisition module (NI cDAQ-9184, National Instruments<sup>®</sup>), which controls nine sampling pumps (one per tank) and a series of gas and water solenoid valves, while receiving input from a central pH electrode (Hach pHD<sup>®</sup> digital electrode calibrated weekly using 2-point pH<sub>NBS</sub> references) and dissolved oxygen probe (Hach LDO<sup>®</sup> Model 2). The software sequentially assesses the pH conditions in each rearing unit (each tank once per hour) by pumping water for ~450 seconds through the housing of the central pH probe, comparing measured pH levels to set-points and then adjusting levels by bubbling standardized amounts 100% CO<sub>2</sub> (bone dry grade, AirGas<sup>®</sup>) or CO<sub>2</sub>-stripped air into the sump of each tank. The software also maintains DO saturation (&gt;8 mg/l) by bubbling in CO<sub>2</sub>-stripped air. LabView logs current pH, temperature, and DO conditions before cycling to the next unit. Temperatures were controlled by thermostats (Aqualogic<sup>®</sup>) that powered submersible heaters or in-line chillers (DeltaStar<sup>®</sup>).</p>
<p>Actual treatment CO<sub>2</sub> levels were determined based on measurements of pH, temperature, salinity, and total alkalinity (<em>A</em><sub>T</sub>). Treatment tanks were sampled three times per experiment for measurements of <em>A</em><sub>T</sub> (μmol kg<sup>-1</sup>). Seawater was siphoned and filtered (to 10 µm) into 300 ml borosilicate bottles. Salinity was measured at the time of sampling using a refractometer. Bottles were stored at 3˚C and measured for <em>A</em><sub>T</sub> within two weeks of sampling using an endpoint titration (Mettler Toledo<sup>®</sup>&nbsp;G20 Potentiometric Titrator). Methodological accuracy (within ±1%) of alkalinity titrations were verified and calibrated using Dr. Andrew Dickson’s (University of California San Diego, Scripps Institution of Oceanography) certified reference material for <em>A</em><sub>T</sub> in seawater. The partial pressure of CO<sub>2</sub> (pCO<sub>2,</sub>; μatm) was calculated in CO2SYS (V2.1, <a href="http://cdiac.ornl.gov/ftp/co2sys">http://cdiac.ornl.gov/ftp/co2sys</a>) based on measured <em>A</em><sub>T</sub>, pH<sub>NBS</sub>, temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973)&nbsp; refit by Dickson and Millero (1987)&nbsp; and Dickson (1990) for KHSO<sub>4</sub>.</p>
<p><strong>Field sampling and experimental designs:</strong><br />
Collections of wild, spawning ripe Atlantic silversides were made during high tide 1-3 days prior to full or new moons during the species spawning season. Adults were caught with a 30 m × 2 m beach seine from local salt marshes and transported live to our laboratory facilities. Ripe adults were held overnight at 20°C in well aerated tanks at low densities with no food and strip spawned the next day.</p>
<p>For each experiment, eggs from 20+ running-ripe females were gently mixed into shallow plastic dishes lined with 1 mm plastic window screening. 20+ males were stripped-spawned together into 500 ml glass beakers, mixed with seawater, stirred, then gently poured into spawning dishes and mixed with eggs for ~15 minutes. Screens were rinsed with seawater to remove unfertilized eggs and then soaked in a 100 ppm buffered iodine (Ovadine<sup>®</sup>) solution for 15 minutes to prevent fungal infection. Experiments were initiated within two hours of fertilization when replicate rearing vessels received precisely 100 embryos. Vessels were filled with clean seawater (filtered to 1 µm and UV sterilized). Optimal salinity (27-31) and light conditions (15 h light:9 h dark) for rearing <em>M. menidia </em>were maintained across experiments. Upon hatching larvae were immediately provided <em>ad libitum</em> rations of newly hatched brine shrimp nauplii (<em>Artemia salina, </em>San Francisco strain, brineshrimpdirect.com) and equal rations of powdered weaning diet (Otohime Marine Fish Diet, size A1, Reed Mariculture®). To quantify hatching survival, one day post first hatch larvae were counted by gently scooping small groups into replacement rearing vessels. For initial hatch measurements, random sub-samples (N = 10) from each replicate were preserved in 5% formaldehyde/freshwater solution buffered with saturated sodium tetraborate. All experiments were terminated when larvae reached ~10 mm standard length (SL). At termination, all survivors were counted and measured for standard length (SL, nearest 0.01 mm) via calibrated digital images (Image Pro Premier<sup>®</sup> V9.0).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- modified parameter names to conform with BCO-DMO naming conventions&nbsp;(replaced spaces with underscores);<br />
- changed date format from mm/dd/yyyy to yyyy/mm/dd;<br />
- replaced "n/a" with "nd";<br />
- replaced spaces with underscores in columns: species, adult_collection_site;<br />
- removed commas from adult_collection_site field;<br />
- replaced original lat/lon values with decimal degree values provided by PI.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
airstone
airstone
PI Supplied Instrument Name: airstone PI Supplied Instrument Description:Elevated CO2 levels were achieved via gas proportioners (ColeParmer) mixing air with 100% CO2 (bone dry grade) that was delivered continuously to the bottom of each replicate rearing container via airstone. Instrument Name: Airstone Instrument Short Name:Airstone Instrument Description: Airstone - Also called an aquarium bubbler, is a piece of aquarium furniture, traditionally a piece of limewood or porous stone, whose purpose is to gradually diffuse air into the tank, eliminating the noise and large bubbles of conventional air filtration systems
DeltaStar chiller
DeltaStar chiller
PI Supplied Instrument Name: DeltaStar chiller PI Supplied Instrument Description:Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
glass submersible heaters
glass submersible heaters
PI Supplied Instrument Name: glass submersible heaters PI Supplied Instrument Description:Target treatment temperatures were controlled by thermostats (Aqualogic) which powered chillers (DeltaStar) or glass submersible heaters to maintain water bath temperatures. Instrument Name: Immersion heater Instrument Short Name:Immersion heater Instrument Description: Submersible heating element for water tanks and aquaria.
dissolved oxygen probe
dissolved oxygen probe
PI Supplied Instrument Name: dissolved oxygen probe Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
handheld pH probe
handheld pH probe
PI Supplied Instrument Name: handheld pH probe PI Supplied Instrument Description:Target pH levels were monitored daily using a handheld pH probes (Orion Ross Ultra pH/ATC Triode with Orion Star A121 pH Portable Meter; Intellical PHC281 pH Electrode with Hach HQ11D Handheld pH/ORP Meter) calibrated bi-weekly with 2-point pHNBS references. Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
refractometer
refractometer
PI Supplied Instrument Name: refractometer PI Supplied Instrument Description:Salinity was measured at the time of sampling using a refractometer. Instrument Name: Refractometer Instrument Short Name:Refractometer Instrument Description: A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.
In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.
beach seine
beach seine
PI Supplied Instrument Name: beach seine PI Supplied Instrument Description:Adults were caught with a 30 m × 2 m beach seine from local salt marshes and transported live to our laboratory facilities. Instrument Name: Seine Net Instrument Short Name: Instrument Description: A seine net is a very long net, with or without a bag in the centre, which is set either from the shore or from a boat for surrounding a certain area and is operated with two (long) ropes fixed to its ends (for hauling and herding the fish).
Seine nets are operated both in inland and in marine waters. The surrounded and catching area depends on the length of the seine and of the hauling lines.
(definition from: fao.org)
Mettler Toledo G20 Potentiometric Titrator
Mettler Toledo G20 Potentiometric Titrator
PI Supplied Instrument Name: Mettler Toledo G20 Potentiometric Titrator PI Supplied Instrument Description:Bottles were stored at 3˚C and measured for AT within two weeks of sampling using an endpoint titration (Mettler Toledo G20 Potentiometric Titrator). Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: Mumford_Cove_Subsurface_Buoy
Mumford_Cove_Subsurface_Buoy
Avery_Point
Avery_Point
laboratory
Mumford_Cove_Subsurface_Buoy
Hannes Baumann
University of Connecticut
Deployment: Poquot_Beach
Poquot_Beach
Avery_Point
Avery_Point
laboratory
Poquot_Beach
Hannes Baumann
University of Connecticut
Avery_Point
Avery_Point
laboratory