<div><p>Symbiodinium culture preparation: Cultures were obtained from the LaJeunesse algal collection at Penn State University. Phylogenetic relationships of these strains are described in Parkinson et al., (2015). Symbiodinium cultures were grown and maintained in liquid media (ASPA-8A, Blank, 1987) at 26 ˚C with fluorescent lights delivering 80-100 μmol quanta m-2 s-1 (measured using a DIVING-PAM equipped with a flat cosine-corrected Fiber Quantum Sensor, Walz, Germany) on a 12:12 hours Light:Dark photoperiod in Innovator 44 incubators (New Brunswick, USA). Symbiodinium cultures of equal starting concentration (3 x 104 cells 126 mL-1) for each strain were grown for 7.5 days at 26 ˚C at logarithmic growth phase prior to experimentation. After 7.5 days of incubation at 26 ˚C, one culture of each strain was subjected to a high temperature treatment of 31 ˚C for two light cycles and one dark cycle (i.e., 1.5 d), whilst another was maintained at 26 ˚C.</p>
<p>Data collection: 100 μL aliquots (8.33 x 104 cells mL-1, calculated as the average of twelve independent hemocytometer measurements) of dark-adapted cultures were then subjected to 5 minutes of elevated temperatures in a thermocycler (Eppendorf Mastercycler Pro S).</p>
<p>Chlorophyll fluorescence parameters were measured immediately after the 5 min temperature exposure using a Fluorometer (see below). Measurements were performed at 25 temperatures (i.e. 26, 30.3, 30.5, 31, 31.7, 32.6, 33.7, 34.8, 35.9, 36.8, 37.6, 38.1, 38.3, 38.8, 38.9, 39.4, 40.1, 41,42, 43, 44, 44.9, 45.6, 46.1, 46.2 ˚C) and replicated three times per Symbiodinium strain per treatment. A modified protocol by Díaz-Almeyda et al., 2011 was used.</p>
<p>Fv/Fm was automatically calculated/determined by fitting each fluorescence transient to the bio-physical model of Kolber et al. (1998) using the FIREPRO software (Satlantic, Version 1.4.3) integrated with the FIRe system.</p>
<p>Reference excitation profile used by FIReView to normalize the variable fluorescence profile: <a href="https://datadocs.bco-dmo.org/docs/medina/Varadero_Reef/data_docs/732890/1/EXCFOP.TXT" target="_blank">EXCFOP.TXT</a><br />
Column 1: incremental sample counter<br />
Column 2: elapsed time measured in microSeconds (uS)<br />
Column 3: reference excitation profile<br />
Column 4: relative fluorescence profile normalized to the reference excitation, or the fluorescence yield<br />
Calibration date: 1/ 1/2002<br />
Time (in seconds) of day reading was taken = 79931</p>
<p>Methodology Reference:<br />
Díaz-Almeyda, E., Thomé, P. E., Hafidi, M. El, and Iglesias-Prieto, R. (2011). Differential stability of photosynthetic membranes and fatty acid composition at elevated temperature in Symbiodinium. Coral Reefs, 30(1), 217–225. <a href="https://doi.org/10.1007/s00338-010-0691-5">https://doi.org/10.1007/s00338-010-0691-5</a>.</p></div>
Symbiodinium Fv/Fm over a temperature gradient
<div><p>This dataset includes the maximum quantum yield (Fv/Fm) of clade A and B Symbiodinium cultures exposed to a range of temperatures.</p>
<p>These data are presented in Mansour et al., 2018.</p></div>
Symbiodinium Fv/Fm over a temperature gradient
<div><p><strong>BCO-DMO Processing Notes:</strong><br />
- added a conventional header with dataset name and description, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p></div>
732890
Symbiodinium Fv/Fm over a temperature gradient
2018-04-04T15:41:57-04:00
2018-04-04T15:41:57-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:732890
Fv/Fm for cultured Clade A & B Symbiodinium with 2 treatments measured over a range of temperatures
Coral photosynthetic endosymbionts (Symbiodinium) are phylogenetically very diverse, yet the extent of inter- and intraspecific functional variation within clades remains largely underexplored. Understanding this variability will be critical for future research on climate change mediated responses. A properly functioning thylakoid membrane is essential for optimal photosynthetic performance both in free-living and in hospite conditions. Here we analyze the thylakoid membrane melting points of 13 Symbiodinium strains from species in Clades B and A, grown at both control (26 °C) and high temperature (31 °C). We observed a broad range of responses to thermal stress regardless of taxonomic rank. Our results support and augment a growing body of literature demonstrating that functional differences among Symbiodinium spp. are as distinct at lower taxonomic levels (i.e. interspecific) as they are among major clades. These findings highlight the importance of assessing the variability of plastid traits across the Symbiodinium tree.
false
Mansour, J., Pollock, F., Díaz-Almeyda, E., Iglesias-Prieto, R., Medina, M. (2018) Fv/Fm for cultured Clade A & B Symbiodinium with 2 treatments measured over a range of temperatures. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-26 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.732890.1 [access date]
true
1
10.1575/1912/bco-dmo.732890.1
false
Symbiodinium
thermal tolerance
phenotypic plasticity
photochemical efficiency
thylakoid membrane
thermal stress
symbiotic dinoflagellates
coral bleaching
2018-03-26
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