http://lod.bco-dmo.org/id/dataset/734915
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-05-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate organic matter (PON, POC, POP) concentrations collected on R/V Roger Revelle cruise RR1604 along the hydrographic line IO9 in the Eastern Indian Ocean from March to April 2016
2020-09-09
publication
2020-09-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.734915.2
Adam Martiny
University of California-Irvine
principalInvestigator
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Martiny, A., Lomas, M. W. (2021) Particulate organic matter (PON, POC, POP) concentrations collected on R/V Roger Revelle cruise RR1604 along the hydrographic line IO9 in the Eastern Indian Ocean from March to April 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 3) Version Date 2020-09-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.734915.3 [access date]
POM concentrations in the Eastern Indian Ocean (IO9N) Dataset Description: <p>Particulate organic nitrogen, carbon, and phosphorus&nbsp;concentrations collected on R/V Roger Revelle cruise RR1604 along the hydrographic line IO9 in the Eastern Indian Ocean from March to April 2016.</p> Methods and Sampling: <p>Water was collected from a circulating seawater system distributed via plastic tubing for POC/PON/POP around 3m deep. An underway system was chosen to vastly increase sampling coverage, replicate number, and sample volume. The water intake is located near the ship sea chest, which may have missed particle production in the subsurface. The circulating seawater was never turned off during the entirety of the transect and kept at a constant flow. Water was passed through a 30 μm nylon mesh (Small Parts #7050-1220-000-12) to remove larger plankton and particles from the sample. Each replicate was collected into a separate 8.5L plastic carboys (Thermo Scientific, Waltham, Massachusetts)<em>.</em> In between stations, carboys were rinsed with 30 μm filtered sample water just prior to collection. Six 8 L seawater samples were divided into POC/PON and POP triplicates. Carboys were placed at ~45° angle to avoid particle settling below the nozzle. Each replicate was passed through a 25 mm pre-combusted (500°C for 5 h) GF/F filter (Whatman, Florham Park, New Jersey) with a nominal pore size of 0.7 μm. The vacuum filtration was an in-line setup with 25 mm filter holders connected to an aspirator pump at -0.08 MPa. POP filters were rinsed with 5 ml of 0.17 M Na₂SO₄ to remove traces of dissolved phosphorus from the filter. All filters were stored in pre-combusted aluminum packets and immediately frozen at -80°C during the cruise and -20°C for shipment.</p>
<p><strong>Particulate Organic Carbon/Nitrogen: </strong>Prior to analysis, the filters for POC and PON were dried according to the JGOFS protocol (Knap et al., 1996). The protocol has a detection range of 0.43-43.13 µM for POC and 0.037-7.39 µM for PON in sea water (Knap et al., 1996). First, the filters were dried in an incubator at 55°C for 24-48 h and then stored in a desiccator with concentrated HCl fumes for 24 h to remove inorganic carbonates. Secondly, the filters were dried again at 55°C for 48 h before being folded and packed into pre-combusted tin capsules (CE Elantech, Lakewood, New Jersey). The packaged filters are analyzed on a CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts) against an atropine standard curve (chemical formula C₁₇H₂₃NO₃).</p>
<p><strong>Particulate Organic Phosphorus:</strong> Particulate organic phosphorus (POP) were analyzed according to a modified ash-hydrolysis protocol (Lomas et al., 2010). Thawed filters were placed in along with a corresponding standard curve of KH₂PO₄. 2 mL of 0.017M MgSO₄ was added to the acid-washed glass vials containing filters and covered with pre-combusted aluminum foil. The vials were placed in an incubator at 90°C for 24 h and then combusted (500°C, 2 h). Once cooled, 5 mL 0.2 M HCl was added and incubated at 90°C for at least 30 min. Next, the supernatant plus 5 mL milli-Q water was mixed with 2:5:1:2 parts ammonium molybdate tetrahydrate, 5N sulfuric acid, potassium antimonyl tartrate, and ascorbic acid for 30 min. Finally the standards and samples were analyzed on a spectrophotometer (Genesys 10vis, Thermo Scientific, Waltham, Massachusetts) at a wavelength of 885 nm to determine POP concentration with an assay detection limit ~0.1 nmol l⁻¹.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559002 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559002
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
207-315-2567 ext 311
60 Bigelow Drive PO Box 380
East Boothbay
ME
04544
United States
mlomas@bigelow.org
pointOfContact
asNeeded
Dataset Version: 3
Unknown
Sample
Station
Latitude
Longitude
ISO_DateTime_UTC
Volume
POC_Rep1
POC_Rep2
POC_Rep3
PON_Rep1
PON_Rep2
PON_Rep3
POP_Rep1
POP_Rep2
POP_Rep3
CN FlashEA 1112 Elemental Analyzer
Genesys 10vis spectrophotometer
theme
None, User defined
sample identification
station
latitude
longitude
ISO_DateTime_UTC
volume of water filtered
particulate organic Carbon (POC)
particulate organic nitrogen
particulate organic phosphorus
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
RR1604
service
Deployment Activity
Hydrographic line IO9, Indian Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Regional variation of phytoplankton diversity and biogeochemical functioning in the subtropical Indian Ocean
https://osprey.bco-dmo.org/project/628972
Collaborative Research: Regional variation of phytoplankton diversity and biogeochemical functioning in the subtropical Indian Ocean
<p><em>Description from NSF award abstract:</em><br />
The Indian Ocean accounts for nearly a fifth of global ocean photosynthesis and is likely a key component in global ocean nutrient and carbon cycles. However, the Indian Ocean may be the least studied major marine body on the planet. Our limited understanding suggests extensive variations in physical and chemical environmental conditions, but how this variation influences biodiversity, nutrient stress, and more broadly regional differences in the functioning of phytoplankton is unknown. To help address these gaps, the investigators will conduct a study by joining an already-funded major research cruise to this region. It will cover a northern region with some of the highest temperatures recorded in open ocean waters, an area around 10°S of predicted (but not tested in situ) iron stress, and a southern subtropical gyre with unique nitrogen to phosphorous(or N:P) ratios. The focus of this project is to quantify and synthesize the interconnectedness of environmental conditions, phytoplankton diversity and genome content, and nutrient biogeochemistry, with the goal of understanding how these may lead to unique biogeochemical regions in Indian Ocean. The research will have broader impacts on many levels. First, it will increase public awareness of the role of phytoplankton on ocean functioning, climate, and people's lives through a new partnership with the Aquarium of the Pacific (AOP), which is the fourth most-attended aquarium in the nation. Secondly, the project will train a postdoctoral scholar as well as a graduate and undergraduate students. Third, the research will dramatically increase our basic knowledge ocean biogeochemistry and in many cases will be the first measurements of their kind made in the Indian Ocean.</p>
<p>This project will address two major questions: How do environmental conditions, phytoplankton diversity, phytoplankton physiology, and biogeochemistry vary across the central Indian Ocean? Are there distinct biogeochemical regimes in the central IO? The researchers hypothesize that environmental conditions, including the relative availability of nitrogen (N) and iron (Fe), lead to three distinct phytoplankton communities and biogeochemical regimes. They will employ a series of advanced analytical tools including high sensitivity measurements of dissolved and particulate nutrients (nitrogen, phosphorus, and iron), genomics, bioassays to test for nutrient stress, and cell-sorting of specific taxa followed by measures of nutrient content and uptake. A focus of this project is to quantify and synthesize the interconnectedness of environmental conditions, phytoplankton diversity and genome content, and nutrient biogeochemistry, and how these lead to unique biogeochemical regions in Indian Ocean. This extensive set of observations can ultimately be linked to ocean models and satellite data to provide a comprehensive view of regional differences in chemistry, biodiversity and phytoplankton biogeochemical functioning in the Indian Ocean.</p>
IO Phytoplankton
largerWorkCitation
project
eng; USA
oceans
Hydrographic line IO9, Indian Ocean
84.7526
110.4547
-31.0335
17.8831
2016-03-22
2016-04-24
GO-SHIP IO9N transect: 20S 95E to 20N 95E
0
BCO-DMO catalogue of parameters from Particulate organic matter (PON, POC, POP) concentrations collected on R/V Roger Revelle cruise RR1604 along the hydrographic line IO9 in the Eastern Indian Ocean from March to April 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/734927.rdf
Name: Sample
Units: unitless
Description: <p>Sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/734928.rdf
Name: Station
Units: unitless
Description: <p>GO-SHIP I9N transect station</p>
http://lod.bco-dmo.org/id/dataset-parameter/734929.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/734930.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/734931.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample collection (UTC); formatted to ISO 8601 standard (yyyy-mm-ddTHH:MM)</p>
http://lod.bco-dmo.org/id/dataset-parameter/734932.rdf
Name: Volume
Units: liters
Description: <p>Volume of sea water filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/734933.rdf
Name: POC_Rep1
Units: micromolar (uM)
Description: <p>Particulate organic carbon replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/734934.rdf
Name: POC_Rep2
Units: micromolar (uM)
Description: <p>Particulate organic carbon replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/734935.rdf
Name: POC_Rep3
Units: micromolar (uM)
Description: <p>Particulate organic carbon replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/734936.rdf
Name: PON_Rep1
Units: micromolar (uM)
Description: <p>Particulate organic nitrogen replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/734937.rdf
Name: PON_Rep2
Units: micromolar (uM)
Description: <p>Particulate organic nitrogen replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/734938.rdf
Name: PON_Rep3
Units: micromolar (uM)
Description: <p>Particulate organic nitrogen replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/734939.rdf
Name: POP_Rep1
Units: nanomolar (nM)
Description: <p>Particulate organic phosphorus replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/734940.rdf
Name: POP_Rep2
Units: nanomolar (nM)
Description: <p>Particulate organic phosphorus replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/734941.rdf
Name: POP_Rep3
Units: nanomolar (nM)
Description: <p>Particulate organic phosphorus replicate 3</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
25996
https://darchive.mblwhoilibrary.org/bitstream/1912/26702/1/dataset-734915_pom-concentrations__v3.tsv
download
25775
https://darchive.mblwhoilibrary.org/bitstream/1912/23838/1/dataset-734915_pom-concentrations__v2.tsv
download
https://doi.org/10.1575/1912/bco-dmo.734915.2
download
onLine
dataset
<p>Water was collected from a circulating seawater system distributed via plastic tubing for POC/PON/POP around 3m deep. An underway system was chosen to vastly increase sampling coverage, replicate number, and sample volume. The water intake is located near the ship sea chest, which may have missed particle production in the subsurface. The circulating seawater was never turned off during the entirety of the transect and kept at a constant flow. Water was passed through a 30 μm nylon mesh (Small Parts #7050-1220-000-12) to remove larger plankton and particles from the sample. Each replicate was collected into a separate 8.5L plastic carboys (Thermo Scientific, Waltham, Massachusetts)<em>.</em> In between stations, carboys were rinsed with 30 μm filtered sample water just prior to collection. Six 8 L seawater samples were divided into POC/PON and POP triplicates. Carboys were placed at ~45° angle to avoid particle settling below the nozzle. Each replicate was passed through a 25 mm pre-combusted (500°C for 5 h) GF/F filter (Whatman, Florham Park, New Jersey) with a nominal pore size of 0.7 μm. The vacuum filtration was an in-line setup with 25 mm filter holders connected to an aspirator pump at -0.08 MPa. POP filters were rinsed with 5 ml of 0.17 M Na₂SO₄ to remove traces of dissolved phosphorus from the filter. All filters were stored in pre-combusted aluminum packets and immediately frozen at -80°C during the cruise and -20°C for shipment.</p>
<p><strong>Particulate Organic Carbon/Nitrogen: </strong>Prior to analysis, the filters for POC and PON were dried according to the JGOFS protocol (Knap et al., 1996). The protocol has a detection range of 0.43-43.13 µM for POC and 0.037-7.39 µM for PON in sea water (Knap et al., 1996). First, the filters were dried in an incubator at 55°C for 24-48 h and then stored in a desiccator with concentrated HCl fumes for 24 h to remove inorganic carbonates. Secondly, the filters were dried again at 55°C for 48 h before being folded and packed into pre-combusted tin capsules (CE Elantech, Lakewood, New Jersey). The packaged filters are analyzed on a CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts) against an atropine standard curve (chemical formula C₁₇H₂₃NO₃).</p>
<p><strong>Particulate Organic Phosphorus:</strong> Particulate organic phosphorus (POP) were analyzed according to a modified ash-hydrolysis protocol (Lomas et al., 2010). Thawed filters were placed in along with a corresponding standard curve of KH₂PO₄. 2 mL of 0.017M MgSO₄ was added to the acid-washed glass vials containing filters and covered with pre-combusted aluminum foil. The vials were placed in an incubator at 90°C for 24 h and then combusted (500°C, 2 h). Once cooled, 5 mL 0.2 M HCl was added and incubated at 90°C for at least 30 min. Next, the supernatant plus 5 mL milli-Q water was mixed with 2:5:1:2 parts ammonium molybdate tetrahydrate, 5N sulfuric acid, potassium antimonyl tartrate, and ascorbic acid for 30 min. Finally the standards and samples were analyzed on a spectrophotometer (Genesys 10vis, Thermo Scientific, Waltham, Massachusetts) at a wavelength of 885 nm to determine POP concentration with an assay detection limit ~0.1 nmol l⁻¹.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- changed date format from mm/dd/yyyy HH:MM to yyyy-mm-ddTHH:MM;<br />
- modified parameter names to conform with BCO-DMO naming conventions;<br />
- 21 June 2018 (v2): replaced original file with new version where values contain 4 digits after the decimal (original version contained only 2 digits);<br />
- 09 Sept 2020 (v3): replaced data with revised version that includes major corrections to the POP values, which were previously reported one magnitude too large.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CN FlashEA 1112 Elemental Analyzer
CN FlashEA 1112 Elemental Analyzer
PI Supplied Instrument Name: CN FlashEA 1112 Elemental Analyzer PI Supplied Instrument Description:CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts) Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Genesys 10vis spectrophotometer
Genesys 10vis spectrophotometer
PI Supplied Instrument Name: Genesys 10vis spectrophotometer PI Supplied Instrument Description:Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: RR1604
RR1604
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel