http://lod.bco-dmo.org/id/dataset/737887
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-05-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Radiocarbon DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin collected from R/V Endeavor cruise EN541 in July 2014
2018-05-30
publication
2018-05-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-03-05
publication
https://doi.org/10.1575/1912/bco-dmo.737887.1
John D. Kessler
University of Rochester
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kessler, J. (2018) Radiocarbon DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin collected from R/V Endeavor cruise EN541 in July 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-05-30 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.737887.1 [access date]
Radiocarbon DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin Dataset Description: <p>Measurements of natural radiocarbon of DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin.</p>
<p>Methane concentration data was published by Weinstein et al. (2016) and Leonte et al., (2017).<br />
The DIC and pH data are published as: Garcia-Tigreros, F. and Kessler, J.D. (2018)</p>
<p>Underway data for cruise EN541 was published and can be access at R2R: <a href="https://doi.org/10.7284/903242" target="_blank">https://doi.org/10.7284/903242</a></p> Methods and Sampling: <p><strong>Methodology:</strong><br />
Dissolved inorganic carbon concentration ([DIC]) samples were measured by acidification and subsequent release of CO<sub>2</sub> with a CO<sub>2</sub> cavity-ringdown spectrometer (CRDS; Picarro G1101-<em>i</em>) detector to an accuracy and precision better than ± 3 umol/kg. Precision is based on a set of 15 duplicates. Accuracy is based on certified reference materials prepared at the Scripps Institution of Oceanography of the University of California, San Diego.</p>
<p>Samples for pH were measured spectrophotometrically on board using an Agilent Cary 100 UV-Visible spectrophotometer and m-cresol purple dye (Clayton and Byrne, 1993; Liu et al., 2011). The pH values are reported on the total scale with a precision better than 0.0002 based on duplicate measurements. The estimated accuracy of spectrophotometric pH is better than 0.002.</p>
<p>Natural radiocarbon samples of DIC (∆<sup>14</sup>C-DIC) were analyzed at the W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory at the University of California, Irvine. The accuracy and precision of the ∆<sup>14</sup>C-DIC measurements is 0.1‰ and 1.7‰, respectively.</p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong><br />
Discrete seawater samples were collected inside Hudson Canyon, US Atlantic Margin aboard the R/V Endeavor from 9 - 13 of July 2014. A total 19 stations and 216 samples were collected along Hudson Canyon and another station was sampled outside the canyon (station E2-HC2). Samples were collected from a bottle Rosette following the procedures in SOP 1 and 6b in Dickson et al., (2007).</p>
<p>[DIC] samples were collected in acid washed and combusted 120 mL serum vials. Vials were allowed to overflow three times their volume, poisoned with 50 uL of a 55 uM HgCl<sub>2</sub>, capped, and store at 4 degrees C&nbsp;until analysis.</p>
<p>∆<sup>14</sup>C-DIC samples were collected in combusted 1L borosilicate bottles, poisoned with 200 uL of a 55 uM HgCl<sub>2</sub>, sealed and stored in the dark at room temperature until isotope analysis.</p>
<p>CH<sub>4</sub> concentration analyses were collected by filling 60, 120, or 160 mL glass serum vials. Vials were filled from the bottom with a length of 1/4" Tygon tubing. Vials were flushed with seven vial volumes of seawater to expel any bubbles and ensure collection of a clean sample, and then were sealed with butyl rubber stoppers taking care not to introduce bubbles during sealing. Immediately after sealing, a 10 mL headspace of ultrahigh purity nitrogen was introduced, displacing an equal volume of water, and the samples were preserved by adding 25 uL of a saturated solution of mercuric chloride. The vials were stored inverted to minimize diffusive gas exchange through the butyl rubber stopper.</p>
<p>CH<sub>4 </sub>concentration measurements were performed using an Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID) following established protocols reported in Weinstein et al. (2016). The uncertainty associated with these measurements was 5.2% based on duplicate measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1318102 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1318102
completed
John D. Kessler
University of Rochester
585-273-4572
227 Hutchison Hall Department of Earth and Environmental Sciences
Rochester
NY
14627
USA
john.kessler@rochester.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_ID
Station_ID
CTD_hex_file
ISO_DateTime
GMT_Date
GMT_Time
Latitude
Longitude
depth
pressure
temp
sal
Density
pH
DIC
CH3
d14C_DIC
C14_DIC_age
W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory
Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID)
Agilent 6850 gas chromatograph
bottle Rosette
CO2 cavity-ringdown spectrometer (CRDS; Picarro G1101-i)
Agilent Cary 100 UV-Visible spectrophotometer
theme
None, User defined
Cruise identfier
station
cast
ISO_DateTime_UTC
date_gmt
time_gmt
latitude
longitude
depth
water pressure
water temperature at measurement depth
salinity
density
pH
dissolved inorganic Carbon
methane
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Accelerator Mass Spectrometer
Flame Ionization Detector
Gas Chromatograph
Niskin bottle
Spectrometer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
EN541
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Investigating the chemical and isotopic kinetics of aerobic methane oxidation
https://osprey.bco-dmo.org/project/737782
Investigating the chemical and isotopic kinetics of aerobic methane oxidation
<p><em>NSF Award Abstract:</em><br />
Roughly 8 billion moles of methane (CH4) were emitted in 83 days during the Deepwater Horizon disaster in the northern Gulf of Mexico in 2010. Interestingly, none of this CH4 was emitted to the atmosphere, but instead stayed dissolved and suspended as "plume" or "intrusion" layers approximately 1000m below the ocean surface. Based on measurements of CH4 concentration and oxidation rates, dissolved oxygen anomalies, and microbial community structure as well as a CH4 geochemical model, it was determined that all the CH4 emitted during this disaster was respired within 120 days of the initial well blowout. In addition, the methanotrophic bacteria responsible for the oxidation of this CH4 appeared to experience all stages of microbial growth, limited only by the availability of CH4. This finding suggests that releases of CH4 into deepwater, be them anthropogenic or natural, will have minimal direct influence on the radiative budget of the atmosphere.</p>
<p>The major weakness in these previous investigations is that CH4 related parameters were only measured at the beginning (May - June 2010) and end (September - October 2010) of this massive CH4 feast, primarily because the rapid demise of CH4 was unanticipated. Thus, the time- and growth phase-dependent understanding of the kinetics of this bloom response is only based on model interpolation between endpoints. A more complete, and measurement-based, understanding of the chemical kinetics is necessary to predict an oceanographic environment's ability to respire large CH4 perturbations. And while measurements of CH4 stable isotopes in theory can be used to assess the extent that the released CH4 has been oxidized, this kinetic isotope effect can only be used in a quantitative fashion if it is known how the isotopic fractionation factor changes with varying chemical and temperature conditions and throughout all stages of the microbial bloom.</p>
<p>In this study, researchers at the Texas A & M University will test two fundamental hypotheses relating to aerobic CH4 oxidation and ultimately produce a thorough characterization of the time-, growth phase-, and temperature-dependency of CH4 oxidation rates, oxidation rate constants, and isotopic fractionation factors. Hypothesis 1: Excluding mixing processes, the bacterial response to a large CH4 perturbation will be limited primarily by the availability of CH4 or dissolved oxygen. Hypothesis 2: Without knowing the stage of microbial growth, measurements of natural stable isotopes of CH4 and dissolved carbon (organic and/or inorganic) cannot be used to assess the extent of CH4 oxidation in situations of large CH4 perturbations. In order to test these hypotheses, with the goal of disproving hypothesis 2, a suite of mesocosm and pure culture incubations will be conducted. Throughout these incubations, concentrations of CH4 and dissolved inorganic carbon as well as their 13C isotopes will be measured in extremely high resolution with new equipment and experimental designs. In addition, dissolved oxygen, nutrient concentrations, trace metals, CH4 oxidation rates, and microbial community structure will be measured.</p>
<p>Broader Impacts. In addition to the normal dissemination of results in publications, meeting presentations, and on a project web site, this work will have strong educational and research impacts with close interactions between the PIs, postdoctoral scholar, graduate student, and undergraduate researchers with collaborations between Texas A&M University and the University of California Santa Barbara. The students will have extended visits at each lab for skill development, knowledge transfer, and general academic growth. During 2010, an informal collaboration was established with Ms. Vicki Soutar, a high school science teacher in Watkinsville, GA, to develop high school science laboratory exercises using real scientific data. This proposed project will involve Ms. Soutar to formalize, enhance, extend, and disseminate the products of this collaboration</p>
Aerobic methane oxidation
largerWorkCitation
project
eng; USA
oceans
-72.42284
-72.20012
39.2878
39.5668
2014-07-09
2014-07-13
0
BCO-DMO catalogue of parameters from Radiocarbon DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin collected from R/V Endeavor cruise EN541 in July 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/737897.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise identifier (EN = R/V Endeavor)</p>
http://lod.bco-dmo.org/id/dataset-parameter/737898.rdf
Name: Station_ID
Units: unitless
Description: <p>Cruise station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/737899.rdf
Name: CTD_hex_file
Units: untiless
Description: <p>CTD hex file name</p>
http://lod.bco-dmo.org/id/dataset-parameter/737900.rdf
Name: ISO_DateTime
Units: unitless
Description: <p>Date and time formatted to ISO8601 standard: yyyy-mm-ddTHH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/737901.rdf
Name: GMT_Date
Units: unitless
Description: <p>Date GMT (yyyy-mm-dd)</p>
http://lod.bco-dmo.org/id/dataset-parameter/737902.rdf
Name: GMT_Time
Units: unitless
Description: <p>Time GMT (HH:MM:SS)</p>
http://lod.bco-dmo.org/id/dataset-parameter/737903.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude North at start of CTD cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/737904.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude West at start of CTD cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/737905.rdf
Name: depth
Units: meters (m)
Description: <p>Niskin bottle depth (m) at sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/737906.rdf
Name: pressure
Units: Digiquartz (db)
Description: <p>Pressure at sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/737907.rdf
Name: temp
Units: Degrees Celsius
Description: <p>Temperature at sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/737908.rdf
Name: sal
Units: Practical (PSU)
Description: <p>Salinity at sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/737909.rdf
Name: Density
Units: kilograms per cubic meter (Kg/m^3)
Description: <p>Density at sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/737910.rdf
Name: pH
Units: Unitless; pH scale
Description: <p>Spectrophotometric pH measured at 25 C using the total hydrogen ion scale</p>
http://lod.bco-dmo.org/id/dataset-parameter/737911.rdf
Name: DIC
Units: micromoles per kilogram (umol/kg)
Description: <p>Dissolved Inorganic Carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/737912.rdf
Name: CH3
Units: nanomolar (nM)
Description: <p>Dissolved methane concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/737913.rdf
Name: d14C_DIC
Units: Per mil (‰)
Description: <p>Natural radiocarbon of DIC</p>
http://lod.bco-dmo.org/id/dataset-parameter/737914.rdf
Name: C14_DIC_age
Units: Years before present
Description: <p>Age of DIC</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
29833
https://darchive.mblwhoilibrary.org/bitstream/1912/25479/1/dataset-737887_hudson-canyon-dic-and-ph__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.737887.1
download
onLine
dataset
<p><strong>Methodology:</strong><br />
Dissolved inorganic carbon concentration ([DIC]) samples were measured by acidification and subsequent release of CO<sub>2</sub> with a CO<sub>2</sub> cavity-ringdown spectrometer (CRDS; Picarro G1101-<em>i</em>) detector to an accuracy and precision better than ± 3 umol/kg. Precision is based on a set of 15 duplicates. Accuracy is based on certified reference materials prepared at the Scripps Institution of Oceanography of the University of California, San Diego.</p>
<p>Samples for pH were measured spectrophotometrically on board using an Agilent Cary 100 UV-Visible spectrophotometer and m-cresol purple dye (Clayton and Byrne, 1993; Liu et al., 2011). The pH values are reported on the total scale with a precision better than 0.0002 based on duplicate measurements. The estimated accuracy of spectrophotometric pH is better than 0.002.</p>
<p>Natural radiocarbon samples of DIC (∆<sup>14</sup>C-DIC) were analyzed at the W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory at the University of California, Irvine. The accuracy and precision of the ∆<sup>14</sup>C-DIC measurements is 0.1‰ and 1.7‰, respectively.</p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong><br />
Discrete seawater samples were collected inside Hudson Canyon, US Atlantic Margin aboard the R/V Endeavor from 9 - 13 of July 2014. A total 19 stations and 216 samples were collected along Hudson Canyon and another station was sampled outside the canyon (station E2-HC2). Samples were collected from a bottle Rosette following the procedures in SOP 1 and 6b in Dickson et al., (2007).</p>
<p>[DIC] samples were collected in acid washed and combusted 120 mL serum vials. Vials were allowed to overflow three times their volume, poisoned with 50 uL of a 55 uM HgCl<sub>2</sub>, capped, and store at 4 degrees C&nbsp;until analysis.</p>
<p>∆<sup>14</sup>C-DIC samples were collected in combusted 1L borosilicate bottles, poisoned with 200 uL of a 55 uM HgCl<sub>2</sub>, sealed and stored in the dark at room temperature until isotope analysis.</p>
<p>CH<sub>4</sub> concentration analyses were collected by filling 60, 120, or 160 mL glass serum vials. Vials were filled from the bottom with a length of 1/4" Tygon tubing. Vials were flushed with seven vial volumes of seawater to expel any bubbles and ensure collection of a clean sample, and then were sealed with butyl rubber stoppers taking care not to introduce bubbles during sealing. Immediately after sealing, a 10 mL headspace of ultrahigh purity nitrogen was introduced, displacing an equal volume of water, and the samples were preserved by adding 25 uL of a saturated solution of mercuric chloride. The vials were stored inverted to minimize diffusive gas exchange through the butyl rubber stopper.</p>
<p>CH<sub>4 </sub>concentration measurements were performed using an Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID) following established protocols reported in Weinstein et al. (2016). The uncertainty associated with these measurements was 5.2% based on duplicate measurements.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- modified parameter names to conform with BCO-DMO naming conventions;<br />
- created date/time column formatted to ISO8601 standard.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory
W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory
PI Supplied Instrument Name: W. M. Keck Carbon Cycle Accelerator Mass Spectrometry Laboratory Instrument Name: Accelerator Mass Spectrometer Instrument Short Name:AMS Instrument Description: An AMS measures "long-lived radionuclides that occur naturally in our environment. AMS uses a particle accelerator in conjunction with ion sources, large magnets, and detectors to separate out interferences and count single atoms in the presence of 1x1015 (a thousand million million) stable atoms, measuring the mass-to-charge ratio of the products of sample molecule disassociation, atom ionization and ion acceleration." AMS permits ultra low-level measurement of compound concentrations and isotope ratios that traditional alpha-spectrometry cannot provide. More from Purdue University: http://www.physics.purdue.edu/primelab/introduction/ams.html Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB17/
Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID)
Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID)
PI Supplied Instrument Name: Agilent 6850 gas chromatograph with a flame ionization detector (GC-FID) Instrument Name: Flame Ionization Detector Instrument Short Name:FID Instrument Description: A flame ionization detector (FID) is a scientific instrument that measures the concentration of organic species in a gas stream. It is frequently used as a detector in gas chromatography. Standalone FIDs can also be used in applications such as landfill gas monitoring, fugitive emissions monitoring and internal combustion engine emissions measurement in stationary or portable instruments.
Agilent 6850 gas chromatograph
Agilent 6850 gas chromatograph
PI Supplied Instrument Name: Agilent 6850 gas chromatograph Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
bottle Rosette
bottle Rosette
PI Supplied Instrument Name: bottle Rosette Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
CO2 cavity-ringdown spectrometer (CRDS; Picarro G1101-i)
CO2 cavity-ringdown spectrometer (CRDS; Picarro G1101-i)
PI Supplied Instrument Name: CO2 cavity-ringdown spectrometer (CRDS; Picarro G1101-i) Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
Agilent Cary 100 UV-Visible spectrophotometer
Agilent Cary 100 UV-Visible spectrophotometer
PI Supplied Instrument Name: Agilent Cary 100 UV-Visible spectrophotometer Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: EN541
EN541
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN541
John D. Kessler
University of Rochester
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel