19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T887,

The three ponds are located in the high marsh (1.43-1.46 m above North American Vertical Datum of 1988) of the Plum Island Ecosystems – Long Term Ecological Research (PIE-LTER) site. Surface sediments were collected weekly for TOC, TN, δ¹³C, and δ¹⁵N (June 25-August 13 and November 11-25, 2014). Cores (5 cm diameter x 2 cm deep) were collected from three 1 m² quadrats placed at random locations along two crisscrossing transects in each pond. Sediments were combined in combusted glass vials to form composite samples and stored (-80 ℃) until analysis. Lipid biomarker compounds were extracted using a modified Bligh & Dyer (1959) method (Spivak and Reeve 2015). Sediments were extracted with a methanol : chloroform : phosphate buffer saline mixture (2:1:0.8, v:v:v) using a microwave-accelerated reaction system (MARS6); samples were heated to 80°C for 10 min with continuous stirring. Samples were then partitioned and the organic phase removed. The total lipid extract was concentrated under N₂ and samples were eluted on silica gel columns with chloroform, acetone (F1/2), and methanol (F3) (Guckert et al. 1985). The F3 (phospholipids) was dried under N₂ and saponified with 0.5 M NaOH at 70°C for 4 h. Saponified samples were acidified and extracted 3 times with hexane. The extract was methylated with acidic methanol (95:5 methanol:HCl) and heated overnight at 70°C to form fatty acid methyl esters (FAME). Samples were analyzed with an Agilent 7890 gas chromatograph with the effluent split ~70:30 between a 5975C mass spectrometer and a flame ionization detector. Compounds were separated on an Agilent DB-5 ms column (60 m, 0.25 mm inner diameter, 0.25 μm film). FAME concentrations were quantified using methyl heneicosanoate as an internal standard. FAs are designated A:BωC, where A is the number of carbon atoms, B is the number of double bonds, and C is the position of the first double bond from the aliphatic ‘ω’ end of the molecule. Iso- and anteiso refer to whether the methyl group of branched compounds is attached to the penultimate or antepenultimate carbon atom.

1b:T88e,Extracted from the NSF award abstract: This project will address how rates of benthic microalgal production respond to eutrophication and geomorphological changes in human-impacted tidal creeks. Excess nutrient loading increases benthic algal biomass and likely stimulates production rates but the magnitude of nutrient and geomorphological effects on rates of production is unknown. Will changes in benthic algal productivity affect algal-bacterial coupling? Furthermore, how is algal-bacterial coupling affected by geomorphological changes, which may be exacerbated by excess nutrient loading but can also occur in pristine marshes? This project will take advantage of the infrastructure of the TIDE project, a long-term saltmarsh eutrophication experiment at the Plum Island Ecosystem - Long Term Ecological Research site in Northeastern Massachusetts. Specifically, the PIs will measure benthic metabolism and examine algal- bacterial coupling in fertilized and ambient nutrient tidal creeks in the first field season. The following field season, they will compare sediment metabolism and carbon dynamics on slumped tidal creek walls (i.e. areas where low marsh has collapsed into the tidal creek) to that on the bottom of tidal creeks. In both years, gross and net production will be determined using an innovative triple oxygen isotope technique and traditional dissolved oxygen and inorganic carbon flux measurements. Comparisons between these methods will be useful in informing studies of sediment metabolism. Lipid biomarkers will be used to characterize the sources of organic matter to creek sediments, and stable isotope analysis of bacterial specific biomarkers to identify the sources of organic carbon utilized by sediment bacteria. The biomarkers will reveal whether sediment bacteria use organic matter substrates, such as benthic microalgal carbon, selectively or in proportion to availability. Overall, results from the proposed study will provide important information about how sediment carbon dynamics in shallow tidal creeks respond to long term eutrophication. Furthermore, findings will enhance understanding of the role of tidal creeks in coastal biogeochemistry.6:["$","$L19",null,{"dataset":{"_id":"738160","description":"

Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA). Data were collected over 11 weeks in the summer and fall of 2014.

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BCO-DMO Data Processing Notes:

-reformatted column names to comply with BCO-DMO standards
\n-reformatted date to \"yyyy-mm-dd\"
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\n","bcodmoProcessing":"","title":"Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA) from 2014.","nickname":"Concentrations of phospholipid-linked fatty acids (PLFAs)","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2018-06-05T04:00:00.000Z","abstract":"Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA). Data were collected over 11 weeks in the summer and fall of 2014.","citation":"Spivak, A. (2018) Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA) from 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-06-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.738160.1 [access date]","citationParts":{"identifier":"doi:10.1575/1912/bco-dmo.738160.1","versionDate":"2018-06-05","versionId":"1","year":"2018","title":"Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA) from 2014.","authors":"Spivak, A.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.1575/1912/bco-dmo.738160.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2014-09-01T04:00:00.000Z","end":"2014-11-30T05:00:00.000Z"},"temporal":{"resolution":"time:unitYear","type":"time:Interval","start":{"value":"1409544000","label":"2014"},"end":{"value":"1417323600","label":"2014"}},"spatial_extent":{"shape":"point","center":{"latitude":42.738349,"longitude":-70.809432},"wkt":" POINT (-70.809432 42.738349)","north":0,"east":0,"south":0,"west":0},"dataset_type":["Other Field Results"],"keywords":["oceans"],"data_files":[{"id":"902166","title":"PLFA.csv","url":"https://datadocs.bco-dmo.org/dataset/738160/file/DwwxDQ5ujypowR/PLFA.csv","showTableUrl":"https://www.bco-dmo.org/dataset/738160/data/view/902166","description":"Primary data file for dataset ID 738160","filename":"PLFA.csv","bytesize":5998,"checksum":{"value":"04c6a3c6f452e3d5ad303159b5823805","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"bcodmo_dataset_738160","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"529580","name":"Amanda Spivak"},"performedAtOrganization":{"_id":"2","name":"Woods Hole Oceanographic Institution"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"650173","name":"Hannah Ake"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"669365","name":"Plum_Island","synonyms":[],"dates":{"start":"2012-07-27T00:00:00.000Z","end":"2012-08-15T00:00:00.000Z"},"platform":{"_id":"658580","name":"shoreside Massachusetts"},"chiefScientists":[]}],"funding":[{"_id":"529582","name":"OCE-1233678","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233678"}],"instruments":[{"_id":"738354","name":"Agilent 7890 gas chromatograph","description":"Used to analyze samples","type_id":"661","type_name":"Gas Chromatograph","type_desc":"Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)","type_acronym":"Gas Chromatograph"},{"_id":"738353","name":"Corer","description":"Used for sediment samples","type_id":"628287","type_name":"Push Corer","type_desc":"Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.\r\n\r\nDescription obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/","type_acronym":""}],"parameters":[{"_id":"738363","name":"Pond","format":"","frictionlessType":"","description":"

Site number

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Alphanumeric string used as the ID for a sampling site

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Week number

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Season at time of sampling

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Tide type

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An association with a community-wide standard parameter has not yet been made.

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Sampling date; yyyy/mm/dd

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date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

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Concentration C12 0

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An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738369","name":"iso_C13","format":"","frictionlessType":"","description":"

Concentration iso C13

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An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738370","name":"anteiso_C13","format":"","frictionlessType":"","description":"

Concentration anteiso C13

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An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738371","name":"C13_0","format":"","frictionlessType":"","description":"

Concentration C13 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738372","name":"iso_C14","format":"","frictionlessType":"","description":"

Concentration iso C14

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738373","name":"C14_0","format":"","frictionlessType":"","description":"

Concentration C14 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738374","name":"iso_C15","format":"","frictionlessType":"","description":"

Concentration iso C15

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738375","name":"anteiso_C15","format":"","frictionlessType":"","description":"

Concentration anteiso C15

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738376","name":"C15_0","format":"","frictionlessType":"","description":"

Concentration C15 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738377","name":"iso_C16","format":"","frictionlessType":"","description":"

Concentration iso C16

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738378","name":"iso_16_1w9c","format":"","frictionlessType":"","description":"

Concentration iso 16 1w9c

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738379","name":"iso_16_1w9t","format":"","frictionlessType":"","description":"

Concentration iso 16 1w9t

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738380","name":"iso_16_1w7","format":"","frictionlessType":"","description":"

Concentration iso 16 1w7

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738381","name":"C16_0","format":"","frictionlessType":"","description":"

Concentration C16 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738382","name":"methyl_10_C16","format":"","frictionlessType":"","description":"

Concentration 10 methyl C16

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738383","name":"iso_C17","format":"","frictionlessType":"","description":"

Concentration C17

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738384","name":"anteiso_C17","format":"","frictionlessType":"","description":"

Concentration anteiso C17

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738385","name":"C17_1","format":"","frictionlessType":"","description":"

Concentration C17 1

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738386","name":"C17_0","format":"","frictionlessType":"","description":"

Concentration C17 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738387","name":"C18_4","format":"","frictionlessType":"","description":"

Concentration C18 4

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738388","name":"C18_2","format":"","frictionlessType":"","description":"

Concentration C18 2

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738389","name":"C18_1","format":"","frictionlessType":"","description":"

Concentration C18 1

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738390","name":"C18_0","format":"","frictionlessType":"","description":"

Concentration C18 0

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An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738391","name":"iso_C19_0","format":"","frictionlessType":"","description":"

Concentration iso C19 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738392","name":"C19_1","format":"","frictionlessType":"","description":"

Concentration C19 1

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738393","name":"C20_4w6","format":"","frictionlessType":"","description":"

Concentration C20 4w6

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738394","name":"C20_5w3","format":"","frictionlessType":"","description":"

Concentration C20 5w3

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738395","name":"C20_3","format":"","frictionlessType":"","description":"

Concentration C20 3

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738396","name":"C20_2","format":"","frictionlessType":"","description":"

Concentration C20 2

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738397","name":"C20_1","format":"","frictionlessType":"","description":"

Concentration C20 1

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738398","name":"C20_0","format":"","frictionlessType":"","description":"

Concentration C20 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738399","name":"C22_6w6","format":"","frictionlessType":"","description":"

Concentration C22 6w6

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738400","name":"C22_6w3","format":"","frictionlessType":"","description":"

Concentration C22 6w3

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738401","name":"C22_5w6","format":"","frictionlessType":"","description":"

Concentration C22 5w6

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738402","name":"C22_5w3","format":"","frictionlessType":"","description":"

Concentration C22 5w3

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738403","name":"C22_1","format":"","frictionlessType":"","description":"

Concentration C22 1

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738404","name":"C22_0","format":"","frictionlessType":"","description":"

Concentration C22 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738405","name":"C24_0","format":"","frictionlessType":"","description":"

Concentration C24 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738406","name":"C25_0","format":"","frictionlessType":"","description":"

Concentration C25 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738407","name":"C26_0","format":"","frictionlessType":"","description":"

Concentration C26 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738408","name":"C28_0","format":"","frictionlessType":"","description":"

Concentration C28 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}},{"_id":"738409","name":"C30_0","format":"","frictionlessType":"","description":"

Concentration C30 0

","units":{"_id":"","name":"per mil"},"type":{"_id":"1073","name":"no_bcodmo_term","description":"

An association with a community-wide standard parameter has not yet been made.

"}}],"related_publications":[{"id":"740026","citation":"Spivak, A. C., Gosselin, K. M., & Sylva, S. P. (2018). Shallow ponds are biogeochemically distinct habitats in salt marsh ecosystems. Limnology and Oceanography. doi:10.1002/lno.10797\r\n","relation_type":["Methods","Results"],"relation_desc":""}],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/738160/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"ERDDAP","items":[{"name":"SUBSET DATA","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.html","contentType":"text/html","actionType":"tool"},{"name":"VIEW TABLE","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.htmlTable","contentType":"text/html","actionType":"view"},{"name":"CSV","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.csv","contentType":"text/csv","actionType":"download"},{"name":"TSV","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.tsv","contentType":"text/tsv","actionType":"download"},{"name":"MATLAB","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.mat","contentType":"text/x-matlab","actionType":"download"},{"name":"netCDF","description":"","url":"https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_738160.nc","contentType":"application/x-netcdf","actionType":"download"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/738160/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/738160/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/738160/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"529583","name":"Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary","acronym":"Benthic_PP_at_TIDE","description":"$1b"}],"archives":[{"id_value":"10.1575/1912/bco-dmo.738160.1","url":"https://doi.org/10.1575/1912/bco-dmo.738160.1","date_assigned":"2019-06-07","version_date":"2018-06-05","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":189},"downloads":{"count":285}}}}]