Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Mitochondiral DNA cytochrome b and radloci genotype identification data from fish sampled in the Belizean Barrier Reef in 2014. Each of the 59 loci appear in two separate columns for each allele except for the mitochondrial DNA, which only appears in one column because there is only one allele at the locus.

We collected tissue samples from 1126 Elacatinus lori individuals at 39 locations across the Belize barrier reef for population genetic analyses. We marked the location of each collection site with a Garmin GPSMAP 76Cx unit. Individuals were caught with slurp guns and we took a small clip from the caudal fin using scissors. All tissue samples were store in 95% EtOH. Genetic data were collected based on standard DNA extractions and a targeted amplicon sequencing approach. Details on all methods can be found in D’Aloia et al. (2017), Conservation Genetics.

To generate the two genotype files, we used a custom Perl script that was specifically developed to run on Cornell University’s servers. All script details and filtering steps are described in detail in D’Aloia et al. 2017, Conservation Genetics. Missing data from genotype columns is denoted by 0.

BCO-DMO Data Processing Notes:

-Added decimal degree lat and lon to data
-Added date to data
-Merged cytb and radloci data into one spreadsheet

Understanding the patterns, causes and consequences of larval dispersal is a major goal of 21st century marine ecology. Patterns of dispersal determine the rates of larval exchange, or connectivity, between populations. Both physical factors (e.g., water movement) and biological factors (e.g., larval behavior) cause variation in population connectivity. Population connectivity, in turn, has major consequences for all aspects of an organism's biology, from individual behavior to metapopulation dynamics, and from evolution within metapopulations to the origin and extinction of species. Further, understanding population connectivity is critical for the design of effective networks of marine reserves, creation of vital tools in conservation, and the development of sustainable fisheries.

Over the last decade, three methods, each of which tells something slightly different, have emerged as leading contenders to provide the greatest insights into population connectivity. First, coupled biophysical models make assumptions regarding water flow, larval behavior and ecology, to predict population connectivity. Second, indirect genetic methods use spatial distributions of allele frequencies to infer population connectivity. Third, direct genetic methods use parentage analyses, tracing recruits to specific adults, to measure population connectivity. Despite advances, lack of integration means that we do not know the predictive skill of biophysical models, or the extent to which patterns of dispersal predict spatial genetic structure. The overall objective of this proposal is to conduct an integrated investigation of population connectivity, using all three methods in one tractable system: the neon goby, Elacatinus lori, on the Belizean Barrier Reef. There are three motives for this choice of study system: i) fourteen highly polymorphic microsatellite loci have been developed, facilitating the assignment of recruits to parents using parentage analyses and the measurement of dispersal; ii) the physical oceanography of the Belizean Barrier Reef is well-studied, facilitating the development and testing of coupled biophysical models; and, iii) E. lori has a relatively small biogeographic range, facilitating analysis of the spatial distribution of allele frequencies throughout its range.

Broader Impacts. The grant will support one postdoc and two graduate students who will be trained in scientific diving, marine fieldwork, population genetics, biophysical modeling, and mathematical modeling, and will gain collaborative research experience. PIs will incorporate research findings in their courses, which cover all these topics. The grant will also broaden participation of under-represented groups by supporting six undergraduates from groups traditionally underrepresented in STEM fields. In each year of the project there will be an All Participants meeting to reinforce the network of participants. A project website will be developed, in English and Spanish, on the theme of larval dispersal and population connectivity. This will include a resource for K-12 marine science educators developed in collaboration with a marine science educator. All PIs will ensure that results are broadly disseminated to the scientific community and general public via appropriate forms of media.