http://lod.bco-dmo.org/id/dataset/740414
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-07-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results of turbulence-exposed sand dollar Dendraster excentricus larvae and their response to a variety of settlement cues
2018-07-19
publication
2018-07-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-12-04
publication
https://doi.org/10.1575/1912/bco-dmo.740414.1
Dr Matthew Ferner
San Francisco State University
principalInvestigator
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Dr Jason Hodin
University of Washington Friday Harbor Laboratories
principalInvestigator
Dr Christopher Lowe
Stanford University - Hopkins
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ferner, M., Gaylord, B., Hodin, J., Lowe, C. (2018) Experimental results of turbulence-exposed sand dollar Dendraster excentricus larvae and their response to a variety of settlement cues. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-19 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.740414.1 [access date]
Figs 1, 2, 3, BMC Zoology Dataset Description: <p>These data are presented in Hodin et al (2018).</p>
<p>Adult Dendraster excentricus were collected from two geographically distinct populations: 1) a subtidal population (~ 100&nbsp;m offshore and 1.5m below the surface at mean lower low water) approximately 30m east of Municipal Pier 2 in downtown Monterey CA, USA; 2) an intertidal population on Crescent Beach in East Sound, Orcas Island, WA, USA. Adults in Monterey were collected by snorkeling&nbsp;and transported to Hopkins Marine Station (“HMS”; Pacific Grove CA, USA). Adults in East Sound were collected at low tide&nbsp;and transported to Friday Harbor Labs (“FHL”; Friday Harbor, WA, USA). In both locations, the sand dollars were partially buried within fine sediments (collected from the adult beds) in flowing seawater aquaria until spawning. (Hodin et al, 2018). These adults served as sources for the larvae used in the experiments.</p>
<p>We raised larval sand dollars (Dendraster excentricus) through their feeding larval stage using standard methods [Strathmann, 1987 and 2014], and on various days after larvae had become competent to settle, we subjected a subset of them to a brief turbulence exposure of a specified high intensity (6 W/kg) conforming to that measured on wave-exposed rocky coasts. Immediately after exposure, we transferred the larvae into different settlement inducing media: Millipore-filtered seawater (MFSW), extracts of sand from inside or outside sand dollar beds, or excess potassium chloride in MFSW. We then quantified settlement as it relates to turbulence exposure and settlement medium. For more detail refer to Hodin et al (2018) and prior papers [Gaylord et al, 2013; Hodin et al, 2015].</p> Methods and Sampling: <p>Figure 1: Early competent sand dollar larvae do not display 'desperate' behaviors at settlement, with or without turbulence exposure. We either exposed D. excentricus larvae 10 day post fertilization (dpf) (reared at ~20°C) to 3 min of 6 W/kg turbulent shear or did not. Then, we transferred exposed and control larvae into one of two settlement conditions: 24 hrs in MFSW alone (left side of graph) or a 1 hr exposure to 40mM excess KCl in MFSW –to assess competence– followed by a 24 hr recovery in MFSW. We detected an effect both of turbulence exposure (F1,12=5.36, p&lt;0.02) and settlement medium (F1,12=228.93, p&lt;0.001) on proportion of larvae settled, but no clear interaction (F1,12=3.41, p=0.09).</p>
<p>Figure 2: Fully competent sand dollar larvae exposed to turbulence are less choosy about settlement substrate, and thus behave like 'desperate' larvae. We either exposed D.&nbsp;excentricus larvae 11&nbsp;dpf&nbsp;(reared at ~20°C) to 3 min of 6 W/kg turbulent shear or did not ("no turbulence"). Then, we transferred exposed and control larvae into one of two settlement conditions: 0% extract of sand from sand dollar aquaria (MFSW; no inducer) or 40% extract of sand from sand dollar aquaria (strong natural inducer), and counted the numbers settled at 1 and 16 hrs. We also exposed a separate set of control (no turbulence) larvae for 1 hr to 40mM excess KCl in MFSW, followed by recovery in MFSW. More than 95% of these latter larvae settled, confirming that the larvae in this experiment were indeed fully competent.</p>
<p>Figure 3: Fully competent sand dollar larvae exposed to turbulence are less choosy about settlement substrate, and thus behave like 'desperate' larvae. We either exposed D. excentricus larvae 40 dpf (reared at ~14°C) to 3 min of 6 W/kg turbulent shear or did not ("no turbulence"). Then, we transferred exposed and control larvae into one of two settlement conditions: 0% extract of sand from sand dollar aquaria (MFSW; no inducer) or 200% extract of sand from a beach without sand dollars (poor quality natural inducer), and counted the numbers settled at 1.5, 3, 7.5 and 19 hrs. We also exposed a separate set of control (no turbulence) larvae to a strong natural inducer (30% extract of sand from sand dollar aquaria; right side of graph). This treatment not only confirms that the larvae were fully competent (100% of larvae settled by 8 hrs), but indicates the expected rate of settlement response in a strong cue as a basis of comparison to the sub-optimal cues described above.</p>
<p>We performed all statistical analyses using R (version 3.4.2) and the lme4 package.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1356966 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1356966
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357033 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357033
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357077 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357077
completed
Dr Matthew Ferner
San Francisco State University
415-338-3724
Romberg Tiburon Center and San Francisco Bay National Estuarine Research Reserve 3150 Paradise Drive
San Francisco
CA
94920
USA
mferner@sfsu.edu
pointOfContact
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
707-875-1940
Bodega Marine Laboratory & Department of Evolution and Ecology 2099 Westshore Road, P.O. Box 247
Bodega Bay
CA
94923
USA
bpgaylord@ucdavis.edu
pointOfContact
Dr Jason Hodin
University of Washington Friday Harbor Laboratories
831-869-6792
620 University Rd.
Friday Harbor
WA
98250
USA
larvador@uw.edu
pointOfContact
Dr Christopher Lowe
Stanford University - Hopkins
Hopkins Marine Station 120 Ocean View Blvd.
Pacific Grove
CA
USA
clowe@stanford.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
figure
competence
date_expt
species
rpm
replicate
dpf
cue
number_larvae
number_settled
number_settled_0hr
number_settled_1hr
number_settled_16hr
number_on_bottom
number_settled_1_5hr
number_settled_8hr
number_settled_18hr
theme
None, User defined
No BCO-DMO term
date
species scientific name (binomial)
replicate
days
treatment
count
featureType
BCO-DMO Standard Parameters
Taylor-Couette system
instrument
BCO-DMO Standard Instruments
Gaylord_Turb-Settlement
service
Deployment Activity
Bodega Marine Lab, Bodega Bay, CA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Turbulence-spurred settlement: Deciphering a newly recognized class of larval response
https://osprey.bco-dmo.org/project/472793
Turbulence-spurred settlement: Deciphering a newly recognized class of larval response
<p><em>Description from NSF award abstract:</em><br />
With this award the investigators will explore a habitat-scale oceanographic process that has the potential to integrate studies of larval delivery with an understanding of how larvae respond to substrate-associated cues. This work will build on published and preliminary data indicating that turbulent shear characteristic of high-energy near shore environments primes larvae to initiate settlement and to transform into the juvenile stage. These prior findings suggest that: 1) Because turbulence intensity varies predictably as a function of the strength of wave breaking and other factors, turbulence could operate as an indicator for larvae of their approach to suitable habitat, providing a link between larger-scale dispersal phenomena, and near-bottom search and selection behaviors; and 2) The larval response to turbulence acts in an unprecedented fashion. In contrast to typical cues, turbulence does not induce settlement directly, but rather spurs otherwise "pre-competent" larvae that are refractory to chemical cues to become "competent", thereby causing them to acquire responsiveness to such cues and undergo settlement. The interdisciplinary team has combined expertise in larval biology, sensory ecology, and organism-flow interactions necessary to address this topic. They will employ a phylogenetically robust approach to explore the scope and adaptive significance of the turbulence response in a widespread and ecologically important class of organisms (echinoids; sea urchins and their relatives), and will determine whether the response is aligned with environmental conditions characteristic of these organisms' adult habitat. They will also test for ecologically important functional consequences of precocious, turbulence-induced settlement. This work will provide a detailed look at an entirely new class of settlement inducer, one with strong potential for changing current conceptualizations of dispersing larval stages, their ability to detect signatures of habitat across multiple scales, and the ways in which organism-level traits might influence population connectivity.</p>
<p>How organisms with dispersing life stages find their way back to adult habitat is a fundamental question in marine ecology. Considerable research has explored links between transport, delivery, settlement, and recruitment, with important advances in knowledge. However, a complete understanding of the larval recruitment process remains elusive. Standard tools for estimating dispersal (e.g., numerical circulation models) have limited spatial resolution, which prevents them from predicting at scales below a few hundred meters how larvae will interact with the shore. Studies investigating larval attachment have focused on chemical, tactile, or near-bottom hydrodynamic cues active across microns to centimeters. The novelty of the present project is that it will focus on processes at habitat scales -- between transport and settlement -- where there is a gap in the understanding of processes.</p>
<p>This project will provide a framework for integrating key concepts of propagule dispersal and settlement, two fundamental but largely disjunct themes in marine science. The understanding that will come from this study will provide key information for ecosystem based management of coastal marine resources. The investigators will develop a "Surfing to Settlement" virtual lab activity based on their research that will be incorporated into the VirtualUrchin web platform, a widely exploited educational resource at Stanford that gets thousands of unique users per month. Through connections to the San Francisco Bay National Estuarine Research Reserve, they will integrate the "Surfing to Settlement" activity into one of NERRs professional development workshops for central California educators, thus disseminating this resource to and gaining valuable feedback from dozens of teachers and thousands of students.</p>
Turbulence-spurred settlement
largerWorkCitation
project
eng; USA
biota
oceans
Bodega Marine Lab, Bodega Bay, CA
-122.8951
-121.8891
36.6017
48.6944
2015-07-23
2017-05-22
Northeast Pacific
0
BCO-DMO catalogue of parameters from Experimental results of turbulence-exposed sand dollar Dendraster excentricus larvae and their response to a variety of settlement cues
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/741000.rdf
Name: figure
Units: unitless
Description: <p>figure number from BMC Zoology publication</p>
http://lod.bco-dmo.org/id/dataset-parameter/741001.rdf
Name: competence
Units: unitless
Description: <p>readiness of the larvae to settle</p>
http://lod.bco-dmo.org/id/dataset-parameter/741002.rdf
Name: date_expt
Units: unitless
Description: <p>date of experiment formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/741003.rdf
Name: species
Units: unitless
Description: <p>species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/741004.rdf
Name: rpm
Units: rotations per minute
Description: <p>rotations per minute of the Taylor-Couette cell; unmanip = no rotation applied</p>
http://lod.bco-dmo.org/id/dataset-parameter/741005.rdf
Name: replicate
Units: unitless
Description: <p>replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/741006.rdf
Name: dpf
Units: days
Description: <p>days post fertilization</p>
http://lod.bco-dmo.org/id/dataset-parameter/741007.rdf
Name: cue
Units: unitless
Description: <p>settlement inducing media; MFSW = Millipore-filtered seawater; KCl = potassium chloride in seawater</p>
http://lod.bco-dmo.org/id/dataset-parameter/741008.rdf
Name: number_larvae
Units: larvae
Description: <p>number of larvae in experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/741009.rdf
Name: number_settled
Units: larvae
Description: <p>number of settled larvae</p>
http://lod.bco-dmo.org/id/dataset-parameter/741010.rdf
Name: number_settled_0hr
Units: larvae
Description: <p>number of settled larvae at time 0</p>
http://lod.bco-dmo.org/id/dataset-parameter/741011.rdf
Name: number_settled_1hr
Units: larvae
Description: <p>number of settled larvae at 1 hour</p>
http://lod.bco-dmo.org/id/dataset-parameter/741012.rdf
Name: number_settled_16hr
Units: larvae
Description: <p>number of settled larvae at 16 hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/741013.rdf
Name: number_on_bottom
Units: larvae
Description: <p>number of settled larvae</p>
http://lod.bco-dmo.org/id/dataset-parameter/741014.rdf
Name: number_settled_1_5hr
Units: larvae
Description: <p>number of settled larvae at 1.5 hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/741015.rdf
Name: number_settled_8hr
Units: larvae
Description: <p>number of settled larvae at 8 hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/741016.rdf
Name: number_settled_18hr
Units: larvae
Description: <p>number of settled larvae at 18 hours</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4043
https://darchive.mblwhoilibrary.org/bitstream/1912/24931/1/dataset-740414_settling-response-sand-dollar-dexcentricus-turbulence-bmc-zoology__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.740414.1
download
onLine
dataset
<p>Figure 1: Early competent sand dollar larvae do not display 'desperate' behaviors at settlement, with or without turbulence exposure. We either exposed D. excentricus larvae 10 day post fertilization (dpf) (reared at ~20°C) to 3 min of 6 W/kg turbulent shear or did not. Then, we transferred exposed and control larvae into one of two settlement conditions: 24 hrs in MFSW alone (left side of graph) or a 1 hr exposure to 40mM excess KCl in MFSW –to assess competence– followed by a 24 hr recovery in MFSW. We detected an effect both of turbulence exposure (F1,12=5.36, p&lt;0.02) and settlement medium (F1,12=228.93, p&lt;0.001) on proportion of larvae settled, but no clear interaction (F1,12=3.41, p=0.09).</p>
<p>Figure 2: Fully competent sand dollar larvae exposed to turbulence are less choosy about settlement substrate, and thus behave like 'desperate' larvae. We either exposed D.&nbsp;excentricus larvae 11&nbsp;dpf&nbsp;(reared at ~20°C) to 3 min of 6 W/kg turbulent shear or did not ("no turbulence"). Then, we transferred exposed and control larvae into one of two settlement conditions: 0% extract of sand from sand dollar aquaria (MFSW; no inducer) or 40% extract of sand from sand dollar aquaria (strong natural inducer), and counted the numbers settled at 1 and 16 hrs. We also exposed a separate set of control (no turbulence) larvae for 1 hr to 40mM excess KCl in MFSW, followed by recovery in MFSW. More than 95% of these latter larvae settled, confirming that the larvae in this experiment were indeed fully competent.</p>
<p>Figure 3: Fully competent sand dollar larvae exposed to turbulence are less choosy about settlement substrate, and thus behave like 'desperate' larvae. We either exposed D. excentricus larvae 40 dpf (reared at ~14°C) to 3 min of 6 W/kg turbulent shear or did not ("no turbulence"). Then, we transferred exposed and control larvae into one of two settlement conditions: 0% extract of sand from sand dollar aquaria (MFSW; no inducer) or 200% extract of sand from a beach without sand dollars (poor quality natural inducer), and counted the numbers settled at 1.5, 3, 7.5 and 19 hrs. We also exposed a separate set of control (no turbulence) larvae to a strong natural inducer (30% extract of sand from sand dollar aquaria; right side of graph). This treatment not only confirms that the larvae were fully competent (100% of larvae settled by 8 hrs), but indicates the expected rate of settlement response in a strong cue as a basis of comparison to the sub-optimal cues described above.</p>
<p>We performed all statistical analyses using R (version 3.4.2) and the lme4 package.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date, reference information<br />
- renamed parameters to BCO-DMO standard<br />
- replaced blank cells with '-' ('not applicable')</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:To generate turbulence intensities (quantified in terms of the energy dissipation rate, in units ofWkg−1) ranging from those found in open ocean waters to those arising on wave-battered coasts, we employed a Taylor–Couette cell [29], an apparatus composed of two vertically oriented, coaxial cylinders separated by a 3.5mm gap that contains seawater (described in greater detail in [1]). We held the stationary inner cylinder, and thus the water in the gap, at 19–21◦C by means of a circulating water stream from a temperature-controlled water bath passing through the cylinder’s interior. During operation, the outer cylinder rotated at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them. At rotation speeds employed for testing sand dollar larvae, the
sheared flow was turbulent [1].
[1]Gaylord B, Hodin J, Ferner MC. 2013 Turbulent shear spurs settlement in larval sea urchins. Proc. Natl Acad. Sci. USA 110, 6901–6906. (doi:10.1073/pnas.
1220680110) Instrument Name: Taylor-Couette system Instrument Short Name: Instrument Description: An apparatus composed of two vertically oriented, coaxial cylinders separated by a gap that contains seawater. During operation, the outer cylinder rotates at a prescribed speed causing relative motion between the cylinders and thereby shearing the seawater between them.
Deployment: Gaylord_Turb-Settlement
Gaylord_Turb-Settlement
lab Bodega Marine Laboratory
laboratory
lab Bodega Marine Laboratory
laboratory