<div><p>Surficial sediments were collected using a multi-corer.</p>
<p>Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. For sediment measurements, peptidase activities were measured in a 1:2 (vol: vol) sediment: autoclaved seawater slurry. 100 micromolar concentrations of substrate were added to triplicate live and single autoclaved killed control incubations. 2 ml of slurry was centrifuged at each timepoint (0, 1h ,2h, and 4 h), filtered, and diluted with 1 ml borate buffer; fluorescence was measured in a 4 ml cuvette. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore.</p>
<p>All incubations were conducted at 0 C in the dark.</p></div>
ARK27-3: sediment MCA
<div><p>This dataset includes peptidase hydrolysis rates from sediments to measure microbial enzyme activities. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.</p></div>
ARK27-3: Sediment MCA hydrolysis rates
<div><p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- reduced decimal precision of rate columns from 9 to 6 places</p></div>
743018
ARK27-3: Sediment MCA hydrolysis rates
2018-07-30T16:11:24-04:00
2018-07-30T16:11:24-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:743018
Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012
This dataset includes peptidase hydrolysis rates from sediments to measure microbial enzyme activities. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.
false
Arnosti, C. (2020) Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.743018.1 [access date]
true
1
10.26008/1912/bco-dmo.743018.1
false
2018-07-24
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