http://lod.bco-dmo.org/id/dataset/743018
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-07-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012
2018-07-24
publication
2018-07-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-04-27
publication
https://doi.org/10.26008/1912/bco-dmo.743018.1
Carol Arnosti
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arnosti, C. (2020) Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.743018.1 [access date]
ARK27-3: sediment MCA Dataset Description: <p>This dataset includes peptidase hydrolysis rates from sediments to measure microbial enzyme activities. Links to archived CTD data are also provided.&nbsp;Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.</p> Methods and Sampling: <p>Surficial sediments were collected using a multi-corer.</p>
<p>Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. For sediment measurements, peptidase activities were measured in a 1:2 (vol: vol) sediment: autoclaved seawater slurry. 100 micromolar concentrations of substrate were added to triplicate live and single autoclaved killed control incubations. 2 ml of slurry was centrifuged at each timepoint (0, 1h ,2h, and 4 h), filtered, and diluted with 1 ml borate buffer; fluorescence was measured in a 4 ml cuvette.&nbsp; Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore.</p>
<p>All incubations were conducted at 0 C in the dark.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1332881 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1332881
completed
Carol Arnosti
University of North Carolina at Chapel Hill
919-962-5754
Dept. of Marine Sciences 3117A Venable/Murray Hall
Chapel Hill
NC
27599-3300
USA
arnosti@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
station_no
depth_no
depth_m
cast_no
ISO_DateTime_UTC
Latitude
Longitude
substrate
timepoint
time_elapsed_hr
rep1_rate
rep2_rate
rep3_rate
average
std_dev
comments
mini fluorometer
theme
None, User defined
station
No BCO-DMO term
depth
cast
ISO_DateTime_UTC
latitude
longitude
time_point
time_elapsed
external_link
featureType
BCO-DMO Standard Parameters
Centrifuge
CTD - profiler
Fluorometer
Multi Corer
instrument
BCO-DMO Standard Instruments
ARK-XXVII-3
service
Deployment Activity
Central Arctic Ocean, Laptev Sea
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
https://osprey.bco-dmo.org/project/712359
Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
<p><em>NSF Award Abstract:</em><br />
Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean.</p>
<p>Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.</p>
Patterns of activities
largerWorkCitation
project
eng; USA
oceans
Central Arctic Ocean, Laptev Sea
31.21
130.5795
79.6502
88.809
2012-08-09
2012-09-22
Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
0
BCO-DMO catalogue of parameters from Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/743028.rdf
Name: station_no
Units: unitless
Description: <p>refers to station number for cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/743029.rdf
Name: depth_no
Units: unitless
Description: <p>sequence of depths sampled (1 is surface; higher numbers at greater depths)</p>
http://lod.bco-dmo.org/id/dataset-parameter/743030.rdf
Name: depth_m
Units: meters
Description: <p>actual depth at which water collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/743031.rdf
Name: cast_no
Units: unitless
Description: <p>cast number (refers to cast of CTD/Niskin bottles on cruise)</p>
http://lod.bco-dmo.org/id/dataset-parameter/743032.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>date and time in ISO format (yyyy-mm-ddTHH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/743033.rdf
Name: Latitude
Units: decimal degreed
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/743034.rdf
Name: Longitude
Units: decimal degreed
Description: <p>longitude; east is postivie</p>
http://lod.bco-dmo.org/id/dataset-parameter/743035.rdf
Name: substrate
Units: unitless
Description: <p>substrates for measurement of enzymatic activities. ara:arabinogalactan; chn:chondroitin sulfate; fuc:fucoidan; lam:laminarin ; pul:pullulan; xyl:xylan</p>
http://lod.bco-dmo.org/id/dataset-parameter/743036.rdf
Name: timepoint
Units: unitless
Description: <p>sampling point post-incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/743037.rdf
Name: time_elapsed_hr
Units: hours
Description: <p>incubation time</p>
http://lod.bco-dmo.org/id/dataset-parameter/743038.rdf
Name: rep1_rate
Units: nanomoles/liter/hour (nmol L-1 h-1)
Description: <p>replicate 1 hydrolysis rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/743039.rdf
Name: rep2_rate
Units: nanomoles/liter/hour (nmol L-1 h-1)
Description: <p>replicate 2 hydrolysis rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/743040.rdf
Name: rep3_rate
Units: nanomoles/liter/hour (nmol L-1 h-1)
Description: <p>replicate 3 hydrolysis rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/743041.rdf
Name: average
Units: nanomoles/liter/hour (nmol L-1 h-1)
Description: <p>average of hydrolysis rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/743042.rdf
Name: std_dev
Units: nanomoles/liter/hour (nmol L-1 h-1)
Description: <p>std deviation of hydrolysis rates</p>
http://lod.bco-dmo.org/id/dataset-parameter/743043.rdf
Name: comments
Units: unitless
Description: <p>url of CTD data in Pangaea database</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11130
https://darchive.mblwhoilibrary.org/bitstream/1912/25688/1/dataset-743018_ark27-3-sediment-mca-hydrolysis-rates__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.743018.1
download
onLine
dataset
<p>Surficial sediments were collected using a multi-corer.</p>
<p>Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. For sediment measurements, peptidase activities were measured in a 1:2 (vol: vol) sediment: autoclaved seawater slurry. 100 micromolar concentrations of substrate were added to triplicate live and single autoclaved killed control incubations. 2 ml of slurry was centrifuged at each timepoint (0, 1h ,2h, and 4 h), filtered, and diluted with 1 ml borate buffer; fluorescence was measured in a 4 ml cuvette.&nbsp; Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore.</p>
<p>All incubations were conducted at 0 C in the dark.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- reduced decimal precision of rate columns from 9 to 6 places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
PI Supplied Instrument Name: Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
mini fluorometer
mini fluorometer
PI Supplied Instrument Name: mini fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
PI Supplied Instrument Name: Instrument Name: Multi Corer Instrument Short Name:Multi Corer Instrument Description: The Multi Corer is a benthic coring device used to collect multiple, simultaneous, undisturbed sediment/water samples from the seafloor. Multiple coring tubes with varying sampling capacity depending on tube dimensions are mounted in a frame designed to sample the deep ocean seafloor. For more information, see Barnett et al. (1984) in Oceanologica Acta, 7, pp. 399-408. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/51/
Cruise: ARK-XXVII-3
ARK-XXVII-3
Community Standard Description
International Council for the Exploration of the Sea
R/V Polarstern
vessel
ARK-XXVII-3
Antje Boetius
Alfred Wegener Institute for Polar and Marine Research
Community Standard Description
International Council for the Exploration of the Sea
R/V Polarstern
vessel