http://lod.bco-dmo.org/id/dataset/743072
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-07-31
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved macronutrient concentrations from incubation experiments performed on RVIB Nathaniel B. Palmer cruise NBP 16-08 from September to October 2016
2018-07-31
publication
2018-07-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-01-03
publication
https://doi.org/10.1575/1912/bco-dmo.743072.1
Kristen Nicolle Buck
University of South Florida
principalInvestigator
Phoebe Dreux Chappell
Old Dominion University
principalInvestigator
Bethany D. Jenkins
University of Rhode Island
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Buck, K., Chappell, P., Jenkins, B. (2018) Dissolved macronutrient concentrations from incubation experiments performed on RVIB Nathaniel B. Palmer cruise NBP 16-08 from September to October 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-31 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.743072.1 [access date]
NBP16-08 incubation dissolved macronutrient concentrations Dataset Description: <p>NBP16-08 incubation dissolved macronutrient concentrations.</p> Methods and Sampling: <p><strong>Sampling and analytical procedures:<br />
Incubation 1</strong> was setup from unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from three consecutive casts to 25-35 m depth at -62.332N, -64.647E and the seawater from each cast was homogenized in three acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys, then distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling. Once filled, incubation bottles were spiked with their treatments in laminar flow hoods in a shipboard trace metal bubble, sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p><strong>Incubation 2</strong>&nbsp;was setup from unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from two consecutive casts to 25-35 m depth at -62.46N, -59.565E and the seawater from each cast was homogenized in three acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys, then distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling. Once filled, incubation bottles were spiked with their treatments in laminar flow hoods in a shipboard trace metal bubble, sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p><strong>Incubation 3</strong>&nbsp;was setup from a combination of filtered and unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from one cast to 25-35 m depth at -62.46N, -59.565E, the location of Incubation 2, filtered through a 0.2 um Acropak membrane capsule filter (Pall) and stored in an acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboy. Additional unfiltered seawater was collected from one cast to 25-35 m depth at -62.332N, -64.647E, the location of Incubation 1, and homogenized in two acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys. The unfiltered water from the Incubation 1 station was distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling and used for one treatment of Incubation 3 (Q in the light, V in the dark). The filtered seawater from the Incubation 2 station was mixed in a 50:50 ratio with the unfiltered water from the Incubation 1 station in acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling and used for the second treatment in Incubation 3 (R in the light, W in the dark). Once filled, incubation bottles were sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p>For all incubations, samples for macronutrients were filtered through sequential 5 um and 0.4 um acid-cleaned polycarbonate track etched filters (Whatman Nuclepore) on Teflon filtration rigs (Savillex) and the filtrate collected in 50 mL Falcon tubes that had been rinsed with distilled water (DIW), soaked overnight in 10% hydrocholoric acid (HCl, Fisher, Trace Metal Grade), rinsed three times with DIW again, dried and rinsed three times with sample prior to filling. Samples were analyzed shipboard, typically within 24 hours, for nitrate+nitrite, phosphate, silicate, and occasionally nitrite. Until analyzed shipboard, samples were stored sealed at 4 ºC in the dark and, following analyses, samples were frozen at -20 ºC and shipped back to the University of South Florida for laboratory-based analyses of nitrite and ammonium, and in some cases again for nitrate+nitrite, phosphate and silicate.</p>
<p>Analytical methodology was based on established methods (Parsons 1984; Gordon et al. 1993) as described for the Lachat 8500 QuickChem in the Lachat QuickChem methods manuals and for the Technicon AAII in the CARIACO methods manual. All labware were either glass or high density polyethylene and were cleaned by an initial distilled water (DIW) rinse, followed by an overnight 10% hydrochloric acid (Fisher, Trace Metal Grade) soak, then rinsed three times with DIW and three times with solvent, analyte or sample prior to fill. Dedicated glassware was used for reagents and standards to avoid cross contamination issues. All reagents were made in high purity Milli-Q (&gt;18 MΩ cm) water.</p>
<p>An artificial seawater matrix was used as the carrier for the analyses on the Lachat QuickChem 8500 at sea and on the Technicon AAII in the lab. 4L batches of artificial seawater were made by dissolving pre-weighed salts (128.50 g NaCl, 28.50 g MgSO4*7H2O, 0.6714 g NaHCO3) in Milli-Q to match Southern Ocean seawater salinity and adjusted to match Southern Ocean seawater pH with 10% hydrochloric acid.</p>
<p>Five-point standard curves were analyzed in duplicate at the beginning and end of each run with duplicate reagent blanks, and quality control checks every seventh sample. Quality control check consisted of either an international reference sample or a quality control sample. The certified nutrient reference samples used, lots CC and CD, were purchased from Kanso Technos in Osaka, Japan. The quality control sample was made from a 20-L homogenized surface Southern Ocean filtered and autoclaved seawater sample. The midpoint standard from the calibration curve was also analyzed every fourteenth sample to check for drift during the runs.</p>
<p>Detection limits for all five parameters on the two instruments used were determined from three times the standard deviation of replicate artificial seawater blanks (n&gt;6). On the Lachat QuickChem 8500, limits of detection were 0.01 uM for nitrate+nitrite, 0.02 uM for phosphate, 0.03 uM for silicate, 0.05 uM for nitrite and 0.5 uM for ammonium. On the Technicon AAII, limits of detection were 0.06 uM for nitrate+nitrite, 0.02 uM for phosphate, 0.2 uM for silicate, 0.01 uM for nitrite, and 0.05 uM for ammonium.</p>
<p>Sample analyses for macronutrients were performed by William Abbott (USF).</p>
<p>Gordon, L.I., Jennings, J., J.C., Ross, A.A. and Krest, J.M. 1993. A suggested protocol for continuous flow automated analysis of seawater nutrients (phosphate, nitrate, nitrite and silicic acid) in the WOCE Hydrographic Program and the Joint Global Ocean Fluxes Study, Methods Manual WHPO 91-1. WOCE Hydrographic Program Office.</p>
<p>Hopkinson, B. M., B. G. Mitchell, R. A. Reynolds, H. Wang, K. E. Selph, C. I. Measures, C. D. Hewes, O. Holm-Hansen, and K. A. Barbeau. 2007. Iron limitation across chlorophyll gradients in the southern Drake Passage: Phytoplankton responses to iron addition and photosynthetic indicators of iron stress. Limnology and Oceanography 52: 2540-2554.</p>
<p>Parsons, T.R., Maita, Y. and Lalli, C.M. 1984. A manual of chemical and biological methods for seawater analysis. Pergammon Press, Oxford, 173 pp.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1443483 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1443483
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1443474 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1443474
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1443646 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1443646
completed
Kristen Nicolle Buck
University of South Florida
541-737-7401
2651 SW Orchard Avenue CEOAS
Corvallis
OR
97331
USA
kristen.buck@oregonstate.edu
pointOfContact
Phoebe Dreux Chappell
Old Dominion University
USF St. Petersburg campus 140 7th Ave. South
St. Petersburg
FL
33701
USA
dreux@usf.edu
pointOfContact
Bethany D. Jenkins
University of Rhode Island
401-874-7551
Department of Cell and Molecular Biology and Graduate School of Oceanography 279 CBLS, 120 Flagg Road
Kingston
RI
02881
USA
bdjenkins@uri.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
INCUBATION
DATE
DAY
ID
TREATMENT
BTLNBR
NO3_NO2
NO3_NO2_STDEV
NO3_NO2_pcnt_RSD
NO3_NO2_INSTR
PO4
PO4_STDEV
PO4_pcnt_RSD
PO4_INSTR
SiO4
SiO4_STDEV
SiO4_pcnt_RSD
SiO4_INSTR
NO2
NO2_STDEV
NO2_pcnt_RSD
NO2_INSTR
NH4
NH4_STDEV
NH4_pcnt_RSD
NH4_INSTR
Lachat QuickChem 8500
Technicon AAII
theme
None, User defined
experiment id
date
incubation time or duration
sample identification
treatment
bottle
nitrate plus nitrite
instrument
reactive phosphorus (PO4)
Silicate (reactive silica)
Nitrite
Ammonium
featureType
BCO-DMO Standard Parameters
Flow Injection Analyzer
Technicon AutoAnalyzer II
instrument
BCO-DMO Standard Instruments
NBP1608
service
Deployment Activity
Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigating Iron-binding Ligands in Southern Ocean Diatom Communities: The Role of Diatom-Bacteria Associations
https://osprey.bco-dmo.org/project/738582
Collaborative Research: Investigating Iron-binding Ligands in Southern Ocean Diatom Communities: The Role of Diatom-Bacteria Associations
<p>This project focuses on an important group of photosynthetic algae in the Southern Ocean (SO), diatoms, and the roles associated bacterial communities play in modulating their growth. Diatom growth fuels the SO food web and balances atmospheric carbon dioxide by sequestering the carbon used for growth to the deep ocean on long time scales as cells sink below the surface. The diatom growth is limited by the available iron in the seawater, most of which is not freely available to the diatoms but instead is tightly bound to other compounds. The nature of these compounds and how phytoplankton acquire iron from them is critical to understanding productivity in this region and globally. The investigators will conduct experiments to characterize the relationship between diatoms, their associated bacteria, and iron in open ocean and inshore waters. Experiments will involve supplying nutrients at varying nutrient ratios to natural phytoplankton assemblages to determine how diatoms and their associated bacteria respond to different conditions. This will provide valuable data that can be used by climate and food web modelers and it will help us better understand the relationship between iron, a key nutrient in the ocean, and the organisms at the base of the food web that use iron for photosynthetic growth and carbon uptake. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The project supports early career senior investigators and the training of graduate and undergraduate students as well as outreach activities with middle school Girl Scouts in Rhode Island, inner city middle and high school age girls in Virginia, and middle school girls in Florida.</p>
<p>The project combines trace metal biogeochemistry, phytoplankton cultivation, and molecular biology to address questions regarding the production of iron-binding compounds and the role of diatom-bacterial interactions in this iron-limited region. Iron is an essential micronutrient for marine phytoplankton. Phytoplankton growth in the SO is limited by a lack of sufficient iron, with important consequences for carbon cycling and climate in this high latitude regime. Some of the major outstanding questions in iron biogeochemistry relate to the organic compounds that bind >99.9% of dissolved iron in surface oceans. The investigators' prior research in this region suggests that production of strong iron-binding compounds in the SO is linked to diatom blooms in waters with high nitrate to iron ratios. The sources of these compounds are unknown but the investigators hypothesize that they may be from bacteria, which are known to produce such compounds for their own use. The project will test three hypotheses concerning the production of these iron-binding compounds, limitations on the biological availability of iron even if present in high concentrations, and the roles of diatom-associated bacteria in these processes. Results from this project will provide fundamental information about the biogeochemical trigger, and biological sources and function, of natural strong iron-binding compound production in the SO, where iron plays a critical role in phytoplankton productivity, carbon cycling, and climate regulation.</p>
Diatom_Bacteria_Ligands
largerWorkCitation
project
eng; USA
oceans
Southern Ocean
-64.647
-59.565
-62.46
-62.332
2016-09-13
2016-10-09
Southern Ocean, Western Antarctic Peninsula 60-65 S, 63 W
0
BCO-DMO catalogue of parameters from Dissolved macronutrient concentrations from incubation experiments performed on RVIB Nathaniel B. Palmer cruise NBP 16-08 from September to October 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/743086.rdf
Name: INCUBATION
Units: unitless
Description: <p>Incubation identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/743087.rdf
Name: DATE
Units: unitless
Description: <p>GMT date when incubation sample was pulled from the incubation bottle for filtering, in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/743088.rdf
Name: DAY
Units: unitless
Description: <p>Day of incubation when sample was collected. Days start from 0 for the day the incubation was setup.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743089.rdf
Name: ID
Units: unitless
Description: <p>Sample identifier for incubation bottle and treatment that sample was collected from.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743090.rdf
Name: TREATMENT
Units: unitless
Description: <p>Incubation treatment identifier. Treatments A-F were exposed to light, treatments G-L were kept in the dark. Treatments were as follows: A and G = +0, control; B and H = +1 nM 57FeCl3; C and I = +4 nM Fe 57FeCl3; D and J = +10 nM Fe 57FeCl3; E and K = +600 pM Vitamin B12; F and L = +4 nM 57FeCl3 and +600 pM Vitamin B12. The R-A, R-B, R-C, R-D, R-E, and R-F bottles were the same light treatments as A, B, C, D, E, and F, respectively, in replicate R-labeled 4-L incubation bottles.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743091.rdf
Name: BTLNBR
Units: unitless
Description: <p>Incubation bottle number. Each 4-L incubation bottle was assigned a unique number from 1-99 across all shipboard incubations.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743092.rdf
Name: NO3_NO2
Units: micromoles per liter (uM)
Description: <p>Concentration of dissolved nitrate+nitrite. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value. Noted as "bdl" when result was below detection limit of instrument(s) used.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743093.rdf
Name: NO3_NO2_STDEV
Units: micromoles per liter (uM)
Description: <p>Standard deviation of replicate nitrate+nitrite concentration measurements. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743094.rdf
Name: NO3_NO2_pcnt_RSD
Units: unitless (percent)
Description: <p>Percent relative standard deviation of replicate nitrate+nitrite concentration measurements. Calculated as NO3_NO2_STDEV divided by NO3_NO2 and multiplied by 100. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743095.rdf
Name: NO3_NO2_INSTR
Units: unitless
Description: <p>Notes which instrument was used for nitrate+nitrite measurements. 1 = Lachat 8500 QuickChem, 2 = Technicon AAII, 3 = Both Lachat 8500 QuickChem and Technicon AAII. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743096.rdf
Name: PO4
Units: micromoles per liter (uM)
Description: <p>Concentration of dissolved reactive phosphate. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value. Noted as "bdl" when result was below detection limit of instrument(s) used.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743097.rdf
Name: PO4_STDEV
Units: micromoles per liter (uM)
Description: <p>Standard deviation of replicate phosphate concentration measurements. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743098.rdf
Name: PO4_pcnt_RSD
Units: unitless (percent)
Description: <p>Percent relative standard deviation of replicate phosphate concentration measurements. Calculated as PO4_STDEV divided by PO4 and multiplied by 100. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743099.rdf
Name: PO4_INSTR
Units: unitless
Description: <p>Notes which instrument was used for phosphate measurements. 1 = Lachat 8500 QuickChem, 2 = Technicon AAII, 3 = Both Lachat 8500 QuickChem and Technicon AAII. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743100.rdf
Name: SiO4
Units: micromoles per liter (uM)
Description: <p>Concentration of dissolved silicate. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value. Noted as "bdl" when result was below detection limit of instrument(s) used.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743101.rdf
Name: SiO4_STDEV
Units: micromoles per liter (uM)
Description: <p>Standard deviation of replicate silicate concentration measurements. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743102.rdf
Name: SiO4_pcnt_RSD
Units: unitless (percent)
Description: <p>Percent relative standard deviation of replicate silicate concentration measurements. Calculated as SiO4_STDEV divided by SiO4 and multiplied by 100. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743103.rdf
Name: SiO4_INSTR
Units: unitless
Description: <p>Notes which instrument was used for silicate measurements. 1 = Lachat 8500 QuickChem, 2 = Technicon AAII, 3 = Both Lachat 8500 QuickChem and Technicon AAII. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743104.rdf
Name: NO2
Units: micromoles per liter (uM)
Description: <p>Concentration of dissolved nitrite. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value. Noted as "bdl" when result was below detection limit of instrument(s) used.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743105.rdf
Name: NO2_STDEV
Units: micromoles per liter (uM)
Description: <p>Standard deviation of replicate nitrite concentration measurements. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743106.rdf
Name: NO2_pcnt_RSD
Units: unitless (percent)
Description: <p>Percent relative standard deviation of replicate nitrite concentration measurements. Calculated as NO2_STDEV divided by NO2 and multiplied by 100. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743107.rdf
Name: NO2_INSTR
Units: unitless
Description: <p>Notes which instrument was used for nitrite measurements. 1 = Lachat 8500 QuickChem, 2 = Technicon AAII, 3 = Both Lachat 8500 QuickChem and Technicon AAII. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743108.rdf
Name: NH4
Units: micromoles per liter (uM)
Description: <p>Concentration of dissolved ammonium. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value. Noted as "bdl" when result was below detection limit of instrument(s) used.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743109.rdf
Name: NH4_STDEV
Units: micromoles per liter (uM)
Description: <p>Standard deviation of replicate ammonium concentration measurements. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743110.rdf
Name: NH4_pcnt_RSD
Units: unitless (percent)
Description: <p>Percent relative standard deviation of replicate ammonium concentration measurements. Calculated as NH4_STDEV divided by NH4 and multiplied by 100. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
http://lod.bco-dmo.org/id/dataset-parameter/743111.rdf
Name: NH4_INSTR
Units: unitless
Description: <p>Notes which instrument was used for ammonium measurements. 1 = Lachat 8500 QuickChem, 2 = Technicon AAII, 3 = Both Lachat 8500 QuickChem and Technicon AAII. Noted as "nda" when no data available for this sample, no sample analyzed, or obviously erroneous data value.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
38211
https://darchive.mblwhoilibrary.org/bitstream/1912/25102/1/dataset-743072_nbp1608-incubation-nutrients__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.743072.1
download
onLine
dataset
<p><strong>Sampling and analytical procedures:<br />
Incubation 1</strong> was setup from unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from three consecutive casts to 25-35 m depth at -62.332N, -64.647E and the seawater from each cast was homogenized in three acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys, then distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling. Once filled, incubation bottles were spiked with their treatments in laminar flow hoods in a shipboard trace metal bubble, sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p><strong>Incubation 2</strong>&nbsp;was setup from unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from two consecutive casts to 25-35 m depth at -62.46N, -59.565E and the seawater from each cast was homogenized in three acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys, then distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling. Once filled, incubation bottles were spiked with their treatments in laminar flow hoods in a shipboard trace metal bubble, sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p><strong>Incubation 3</strong>&nbsp;was setup from a combination of filtered and unfiltered seawater collected aboard the R/V/I/B Nathanial B. Palmer using a SeaBird GEOTRACES style SBE32 rosette system deployed on a conducting Kevlar line with OceanTestEquipment, Inc. X-Niskin samplers modified for trace element sampling. Seawater was collected from one cast to 25-35 m depth at -62.46N, -59.565E, the location of Incubation 2, filtered through a 0.2 um Acropak membrane capsule filter (Pall) and stored in an acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboy. Additional unfiltered seawater was collected from one cast to 25-35 m depth at -62.332N, -64.647E, the location of Incubation 1, and homogenized in two acid-cleaned and Milli-Q (&gt;18.2 MΩ cm) conditioned 50-L polypropylene carboys. The unfiltered water from the Incubation 1 station was distributed into acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling and used for one treatment of Incubation 3 (Q in the light, V in the dark). The filtered seawater from the Incubation 2 station was mixed in a 50:50 ratio with the unfiltered water from the Incubation 1 station in acid-cleaned and Milli-Q conditioned 4-L polycarbonate incubation bottles that were rinsed three times with the seawater prior to filling and used for the second treatment in Incubation 3 (R in the light, W in the dark). Once filled, incubation bottles were sealed with the caps wrapped in parafilm and placed in a 2 ºC temperature-controlled lit incubator van with 24 hour blue light (Hopkinson et al. 2007). Dark treatment bottles were placed in the same van but wrapped in heavy-duty black construction garbage bags.</p>
<p>For all incubations, samples for macronutrients were filtered through sequential 5 um and 0.4 um acid-cleaned polycarbonate track etched filters (Whatman Nuclepore) on Teflon filtration rigs (Savillex) and the filtrate collected in 50 mL Falcon tubes that had been rinsed with distilled water (DIW), soaked overnight in 10% hydrocholoric acid (HCl, Fisher, Trace Metal Grade), rinsed three times with DIW again, dried and rinsed three times with sample prior to filling. Samples were analyzed shipboard, typically within 24 hours, for nitrate+nitrite, phosphate, silicate, and occasionally nitrite. Until analyzed shipboard, samples were stored sealed at 4 ºC in the dark and, following analyses, samples were frozen at -20 ºC and shipped back to the University of South Florida for laboratory-based analyses of nitrite and ammonium, and in some cases again for nitrate+nitrite, phosphate and silicate.</p>
<p>Analytical methodology was based on established methods (Parsons 1984; Gordon et al. 1993) as described for the Lachat 8500 QuickChem in the Lachat QuickChem methods manuals and for the Technicon AAII in the CARIACO methods manual. All labware were either glass or high density polyethylene and were cleaned by an initial distilled water (DIW) rinse, followed by an overnight 10% hydrochloric acid (Fisher, Trace Metal Grade) soak, then rinsed three times with DIW and three times with solvent, analyte or sample prior to fill. Dedicated glassware was used for reagents and standards to avoid cross contamination issues. All reagents were made in high purity Milli-Q (&gt;18 MΩ cm) water.</p>
<p>An artificial seawater matrix was used as the carrier for the analyses on the Lachat QuickChem 8500 at sea and on the Technicon AAII in the lab. 4L batches of artificial seawater were made by dissolving pre-weighed salts (128.50 g NaCl, 28.50 g MgSO4*7H2O, 0.6714 g NaHCO3) in Milli-Q to match Southern Ocean seawater salinity and adjusted to match Southern Ocean seawater pH with 10% hydrochloric acid.</p>
<p>Five-point standard curves were analyzed in duplicate at the beginning and end of each run with duplicate reagent blanks, and quality control checks every seventh sample. Quality control check consisted of either an international reference sample or a quality control sample. The certified nutrient reference samples used, lots CC and CD, were purchased from Kanso Technos in Osaka, Japan. The quality control sample was made from a 20-L homogenized surface Southern Ocean filtered and autoclaved seawater sample. The midpoint standard from the calibration curve was also analyzed every fourteenth sample to check for drift during the runs.</p>
<p>Detection limits for all five parameters on the two instruments used were determined from three times the standard deviation of replicate artificial seawater blanks (n&gt;6). On the Lachat QuickChem 8500, limits of detection were 0.01 uM for nitrate+nitrite, 0.02 uM for phosphate, 0.03 uM for silicate, 0.05 uM for nitrite and 0.5 uM for ammonium. On the Technicon AAII, limits of detection were 0.06 uM for nitrate+nitrite, 0.02 uM for phosphate, 0.2 uM for silicate, 0.01 uM for nitrite, and 0.05 uM for ammonium.</p>
<p>Sample analyses for macronutrients were performed by William Abbott (USF).</p>
<p>Gordon, L.I., Jennings, J., J.C., Ross, A.A. and Krest, J.M. 1993. A suggested protocol for continuous flow automated analysis of seawater nutrients (phosphate, nitrate, nitrite and silicic acid) in the WOCE Hydrographic Program and the Joint Global Ocean Fluxes Study, Methods Manual WHPO 91-1. WOCE Hydrographic Program Office.</p>
<p>Hopkinson, B. M., B. G. Mitchell, R. A. Reynolds, H. Wang, K. E. Selph, C. I. Measures, C. D. Hewes, O. Holm-Hansen, and K. A. Barbeau. 2007. Iron limitation across chlorophyll gradients in the southern Drake Passage: Phytoplankton responses to iron addition and photosynthetic indicators of iron stress. Limnology and Oceanography 52: 2540-2554.</p>
<p>Parsons, T.R., Maita, Y. and Lalli, C.M. 1984. A manual of chemical and biological methods for seawater analysis. Pergammon Press, Oxford, 173 pp.</p>
Specified by the Principal Investigator(s)
<p>Data were processed using Omnion 4.2 software.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- changed date format from m/dd/yyyy to yyyy-mm-dd;<br />
- replaced % with "pcnt" in param names;<br />
- replaced spaces with underscores in TREATMENT column;<br />
- combined 3 separate files into one dataset.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
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info@bco-dmo.org
http://www.bco-dmo.org
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For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Lachat QuickChem 8500
Lachat QuickChem 8500
PI Supplied Instrument Name: Lachat QuickChem 8500 PI Supplied Instrument Description:Lachat QuickChem 8500 series 2, 4 channel analyzer Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
Technicon AAII
Technicon AAII
PI Supplied Instrument Name: Technicon AAII PI Supplied Instrument Description:Technicon AAII Instrument Name: Technicon AutoAnalyzer II Instrument Short Name: Instrument Description: A rapid flow analyzer that may be used to measure nutrient concentrations in seawater. It is a continuous segmented flow instrument consisting of a sampler, peristaltic pump, analytical cartridge, heating bath, and colorimeter. See more information about this instrument from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0291/
Cruise: NBP1608
NBP1608
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1608
Bethany D. Jenkins
University of Rhode Island
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel