http://lod.bco-dmo.org/id/dataset/743763
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-08-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity.
2018-08-20
publication
2018-08-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-10
publication
https://doi.org/10.1575/1912/bco-dmo.743763.1
Erik Sotka
Grice Marine Laboratory - College of Charleston
principalInvestigator
Courtney Murren
College of Charleston
principalInvestigator
Allan Strand
Grice Marine Laboratory - College of Charleston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sotka, E., Murren, C., Strand, A. (2018) Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-08-20 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.743763.1 [access date]
Palatability of non-native alga Dataset Description: <p>Bleaching scores of introduced seaweed when stressed by heat, cold, or low salinity.</p> Methods and Sampling: <p>These<em> </em>data are described in detail in Sotka et al., 2018. From May until October 2015, we collected live thalli at each of 40 populations: 15 Japanese (six source and nine nonsource), five western North American, 10 eastern North American, and 10 European populations. We exposed a haphazardly chosen set of approximately 20 thalli per population to extreme heat (1, 2, and 4 hr in 40°C), extreme cold (45, 75, and 105 min at −20°C), and low salinities (8 days at 0, 5, and 10 ppt). For the heat tolerance assay, four apices per thallus were individually placed into unsealed 2- ml microcentrifuge tubes containing 175 μl of seawater. A control apex was kept in a 15°C growth chamber, while three apices were placed in a 40°C water bath for 1, 2, and 4 hr. For the cold tolerance assay, four apices were independently placed into sealed 250 μl PCR tubes containing 200 μl seawater. A control apex was kept in a 15°C incubator, while three apices were placed in a −20° C freezer for 45, 75, and 105 min. Apices were then placed into randomly assigned wells of 12- well tissue culture plates (2.21- cm- diameter wells) filled with 4 ml of seawater and incubated at 15°C for 8 days with a 12- hr light/dark cycle. For the salinity tolerance assay, four apices were independently placed directly into the 12- well tissue culture plates filled with 4 ml of seawater at a titration of salinities diluted to 0, 5, and 10 ppt, with 30 ppt serving as a control. Every 2 days for 8 days, we changed water and assessed thallus bleaching (i.e., loss of photosynthetic pigments; Figure S2) as a proxy of mortality, itself an important component of fitness. We generated a Bleaching Score (BS), where “1” indicated no bleaching, “2” indicated partial bleaching or color change to pink or white, and “3” indicated full bleaching.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357386 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357386
completed
Erik Sotka
Grice Marine Laboratory - College of Charleston
843-953-9191
Grice Marine Laboratory 205 Fort Johnson Road
Charleston
SC
29412
USA
SotkaE@cofc.edu
pointOfContact
Courtney Murren
College of Charleston
843-953-8077
Dept. of Biology
Charleston
SC
29424
USA
murenc@cofc.edu
pointOfContact
Allan Strand
Grice Marine Laboratory - College of Charleston
843-953-9189
Grice Marine Laboratory, 66 George St. Room 216
Charleston
SC
29424
USA
stranda@cofc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
assay
ind_type
pop
ind
trt
day
bleach_score
theme
None, User defined
sample description
sample identification
treatment
day of month
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Detecting genetic adaptation during marine invasions
https://osprey.bco-dmo.org/project/472708
Detecting genetic adaptation during marine invasions
<p><em>Description from NSF award abstract:</em><br />
Biological introductions, defined as the establishment of species in geographic regions outside the reach of their natural dispersal mechanisms, have dramatically increased in frequency during the 20th century and are now altering community structure and ecosystem function of virtually all marine habitats. To date, studies on marine invasions focus principally on demographic and ecological processes, and the importance of evolutionary processes has been rarely tested. This knowledge gap has implications for management policies, which attempt to prevent biological introductions and mitigate their impacts. The Asian seaweed <em>Gracilaria vermiculophylla</em> has been introduced to every continental margin in the Northern Hemisphere, and preliminary data indicate that non-native populations are both more resistant to heat stress and resistant to snail herbivory. The project will integrate population genetics, field survey and common-garden laboratory experiments to comprehensively address the role of rapid evolutionary adaptation in the invasion success of this seaweed. Specifically, the PIs will answer the following. What is the consequence of introductions on seaweed demography and mating systems? How many successful introductions have occurred in North America and Europe? Where did introduced propagules originate? Do native, native-source and non-native locations differ in environmental conditions? Do native, native-source and non-native populations differ in phenotype?</p>
<p>The intellectual merit of this project is based on three gaps in the literature. First, while biological invasions are widely recognized as a major component of global change, there are surprisingly few studies that compare native and non-native populations in their biology or ecology. Native and non-native populations will be surveyed in a similar manner, allowing assessment of differences in population dynamics, mating system, epifaunal and epiphytic communities, and the surrounding abiotic and biotic environment. Second, <em>G. vermiculophylla</em> exhibits a life cycle typical of other invasive species (including some benthic invertebrates), yet we still lack data on the effects of decoupling the haploid and diploid stages on genetic structure, and in turn, on the evolvability of their populations. Finally, this project will provide unequivocal evidence of an adaptive shift in a marine invasive. To our knowledge, such evolutionary change has been described previously for only a complex of marine copepod species. <em>G. vermiculophylla</em> will serve as a model for understanding evolution in other nuisance invasions, and perhaps lead to novel methods to counter future invasions or their spread.</p>
Genetic Adaptation Marine Inv
largerWorkCitation
project
eng; USA
oceans
-123.04
-64.05
-64.77
38.32
1996-01-01
2014-12-31
Estuaries of NW and NE Pacific; estuaries of NW and NE Atlantic
0
BCO-DMO catalogue of parameters from Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/744015.rdf
Name: assay
Units: unitless
Description: <p>Type of assay; heat, cold, or salinity assay.</p>
http://lod.bco-dmo.org/id/dataset-parameter/744016.rdf
Name: ind_type
Units: unitless
Description: <p>Common garden vs field collected samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/744017.rdf
Name: pop
Units: unitless
Description: <p>Three letter abbreviation; see Sotka et al. 2018 Evolutionary Applications</p>
http://lod.bco-dmo.org/id/dataset-parameter/744018.rdf
Name: ind
Units: unitless
Description: <p>Individual ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/744019.rdf
Name: trt
Units: unitless
Description: <p>Levels of treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/744020.rdf
Name: day
Units: days
Description: <p>Day 0, 2, 4, 6, 8 after treatment started</p>
http://lod.bco-dmo.org/id/dataset-parameter/744021.rdf
Name: bleach_score
Units: unitless
Description: <p>1=healthy; 2=partially healthy; 3=bleached</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2001449
https://darchive.mblwhoilibrary.org/bitstream/1912/24236/1/dataset-743763_bleaching-scores-introduced-seaweed__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.743763.1
download
onLine
dataset
<p>These<em> </em>data are described in detail in Sotka et al., 2018. From May until October 2015, we collected live thalli at each of 40 populations: 15 Japanese (six source and nine nonsource), five western North American, 10 eastern North American, and 10 European populations. We exposed a haphazardly chosen set of approximately 20 thalli per population to extreme heat (1, 2, and 4 hr in 40°C), extreme cold (45, 75, and 105 min at −20°C), and low salinities (8 days at 0, 5, and 10 ppt). For the heat tolerance assay, four apices per thallus were individually placed into unsealed 2- ml microcentrifuge tubes containing 175 μl of seawater. A control apex was kept in a 15°C growth chamber, while three apices were placed in a 40°C water bath for 1, 2, and 4 hr. For the cold tolerance assay, four apices were independently placed into sealed 250 μl PCR tubes containing 200 μl seawater. A control apex was kept in a 15°C incubator, while three apices were placed in a −20° C freezer for 45, 75, and 105 min. Apices were then placed into randomly assigned wells of 12- well tissue culture plates (2.21- cm- diameter wells) filled with 4 ml of seawater and incubated at 15°C for 8 days with a 12- hr light/dark cycle. For the salinity tolerance assay, four apices were independently placed directly into the 12- well tissue culture plates filled with 4 ml of seawater at a titration of salinities diluted to 0, 5, and 10 ppt, with 30 ppt serving as a control. Every 2 days for 8 days, we changed water and assessed thallus bleaching (i.e., loss of photosynthetic pigments; Figure S2) as a proxy of mortality, itself an important component of fitness. We generated a Bleaching Score (BS), where “1” indicated no bleaching, “2” indicated partial bleaching or color change to pink or white, and “3” indicated full bleaching.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>-Reformatted column names to comply with BCO-DMO standards</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact