http://lod.bco-dmo.org/id/dataset/745492
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-09-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 1B: Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments
2018-09-04
publication
2018-09-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-31
publication
https://doi.org/10.26008/1912/bco-dmo.745492.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U. (2020) Series 1B: Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.745492.1 [access date]
1B: T. pseudonana growth: light and temperature effects Dataset Description: <p>This dataset reports supplemental experiments that were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335. Experiments were conducted at three temperatures and two light levels. Optical density measurements, dark-adapted Instantaneous Chlorophyll Fluorescence, and the quantum yield and cell concentrations were determined daily. Particulate organic carbon and nitrogen as well as Chl a were measured when volume allowed.</p> Methods and Sampling: <p>T. pseudonana was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975) with 50mL autoclaved natural filtered seawater per liter added. Experiments were conducted at 13.5, 22, and 26˚C. Cultures were kept on a 12-hour light 12-hour dark cycle under light intensities ranging from 35 µmol m-2 s-1 to 350 µmol m-2 s-1. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope. Cell concentrations on day 0 were calculated based on the inoculum and the dilution. Particulate organic carbon and nitrogen as well as Chl a were measured when volume allowed. Combusted 25mm GFF were prepared for particulate organic carbon and particulate organic nitrogen (POC, PON) and measured in a CEC44OHA elemental analyzer (Control Equipment). Chl a was collected on 47mm 0.45 HAWP filters, then extracted in 90% acetone for 24hrs and measured in a fluorometer (Turner 700).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
asNeeded
Dataset Version: 1
Unknown
expt
temp
light_level
date_expt
light
day
avg_OD680
avg_OD720
F0
cell_counts
QY
Chla_repl_1
Chla_repl_2
POC_repl_1
POC_repl_2
PON_repl_1
PON_repl_2
Multicultivator MC-1000 OD (Qubit Systems)
Z985 Cuvette Aquapen (Qubit Systems)
theme
None, User defined
experiment id
water temperature at measurement depth
No BCO-DMO term
date
irradiance
day of month
optical_density
fluorescence
cell_concentration
Fv to Fm ratio
chlorophyll a
particulate organic Carbon (POC)
particulate organic nitrogen
featureType
BCO-DMO Standard Parameters
Cell Cultivator
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2017-05-08
2017-07-07
0
BCO-DMO catalogue of parameters from Series 1B: Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335 conducted to supplement series 1A experiments
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/745501.rdf
Name: expt
Units: unitless
Description: <p>experiment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/745502.rdf
Name: temp
Units: degrees Celsius
Description: <p>experimental temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/745503.rdf
Name: light_level
Units: unitless
Description: <p>experimental light level</p>
http://lod.bco-dmo.org/id/dataset-parameter/745504.rdf
Name: date_expt
Units: unitless
Description: <p>date of experiment formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/745505.rdf
Name: light
Units: micromoles/square meter/second (umol m-2 s-1)
Description: <p>irradiance</p>
http://lod.bco-dmo.org/id/dataset-parameter/745506.rdf
Name: day
Units: day
Description: <p>sampling day where day 0 equals inoculation</p>
http://lod.bco-dmo.org/id/dataset-parameter/745507.rdf
Name: avg_OD680
Units: instrument units
Description: <p>optical density at 680 nm; averaged from 7 measurements taken automatically in 10 minute intervals</p>
http://lod.bco-dmo.org/id/dataset-parameter/745508.rdf
Name: avg_OD720
Units: instrument units
Description: <p>optical density at 720 nm; averaged from 7 measurements taken automatically in 10 minute intervals</p>
http://lod.bco-dmo.org/id/dataset-parameter/745509.rdf
Name: F0
Units: instrument units
Description: <p>instantaneous chlorophyll fluorescence (F0)</p>
http://lod.bco-dmo.org/id/dataset-parameter/745510.rdf
Name: cell_counts
Units: cells/milliliter
Description: <p>cell concentrations obtained microscopically</p>
http://lod.bco-dmo.org/id/dataset-parameter/745511.rdf
Name: QY
Units: dimensionless
Description: <p>the quantum yield: QY=Fv/Fm; where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity</p>
http://lod.bco-dmo.org/id/dataset-parameter/745512.rdf
Name: Chla_repl_1
Units: microgram/liter
Description: <p>total chlorophyll a measured fluorometrically for replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/745513.rdf
Name: Chla_repl_2
Units: microgram/liter
Description: <p>total chlorophyll a measured fluorometrically for replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/745514.rdf
Name: POC_repl_1
Units: microgram/liter
Description: <p>Particulate organic carbon for replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/745515.rdf
Name: POC_repl_2
Units: microgram/liter
Description: <p>Particulate organic carbon for replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/745516.rdf
Name: PON_repl_1
Units: microgram/liter
Description: <p>Particulate orgainic nitrogen for replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/745517.rdf
Name: PON_repl_2
Units: microgram/liter
Description: <p>Particulate orgainic nitrogen for replicate #2</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13628
https://darchive.mblwhoilibrary.org/bitstream/1912/26146/1/dataset-745492_series-1b-light-and-temperature-growth-follow-expts__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.745492.1
download
onLine
dataset
<p>T. pseudonana was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975) with 50mL autoclaved natural filtered seawater per liter added. Experiments were conducted at 13.5, 22, and 26˚C. Cultures were kept on a 12-hour light 12-hour dark cycle under light intensities ranging from 35 µmol m-2 s-1 to 350 µmol m-2 s-1. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope. Cell concentrations on day 0 were calculated based on the inoculum and the dilution. Particulate organic carbon and nitrogen as well as Chl a were measured when volume allowed. Combusted 25mm GFF were prepared for particulate organic carbon and particulate organic nitrogen (POC, PON) and measured in a CEC44OHA elemental analyzer (Control Equipment). Chl a was collected on 47mm 0.45 HAWP filters, then extracted in 90% acetone for 24hrs and measured in a fluorometer (Turner 700).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- replaced blank cells with 'nd' (no data)<br />
- combined data for all four experiments into one table (dataset)<br />
- added columns for experiment identifier, temperature, light level and date of experiment<br />
- reformatted integers to remove embedded commas</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Multicultivator MC-1000 OD (Qubit Systems)
Multicultivator MC-1000 OD (Qubit Systems)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubations and optical density measurements. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
Z985 Cuvette Aquapen (Qubit Systems)
Z985 Cuvette Aquapen (Qubit Systems)
PI Supplied Instrument Name: Z985 Cuvette Aquapen (Qubit Systems) PI Supplied Instrument Description:Used to measure instantaneous chlorophyll fluorescence (F0) and quantum Yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity).
A 20-minute dark adaptation time was used.
AquaPen settings: f = 30, F=71, A = 50. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/