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            <gco:CharacterString>Cite this dataset as: Roman, M., Kimmel, D., Pierson, J., Huebert, K. (2018) Mesozooplankton sample data from R/V Pelican cruises PE03-NGOMEX, PE04-NGOMEX, PE06-NGOMEX, PE07-NGOMEX, PE09-05 in the Northern Gulf of Mexico from 2003-2008. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2018-10-22 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.749705 [access date]</gco:CharacterString>
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        <gco:CharacterString>Analysis of mesozooplankton from pump samples Dataset Description: &amp;lt;p&amp;gt;Subsets of these data were published in the papers listed in the &amp;quot;Related Resources&amp;quot; section.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Related dataset:&amp;lt;br /&amp;gt;
* ScanFish Optical Plankton Counter (OPC) data:&amp;amp;nbsp;https://www.bco-dmo.org/dataset/746081&amp;lt;/p&amp;gt; Methods and Sampling: Methodology: 
Plankton samples were collected from discrete depths during CTD casts using a high-capacity, diaphragm pump with an intake hose mounted to the CTD Rosette. Subsamples of sample contents were manually counted, identified, and measured in the laboratory

Sampling and analytical procedures: 
For each sample, the pump was run for a timed period of nominally 5 min. The flow rate of the pump was usually measured immediately prior to sampling, by recording the time required to fill a barrel of known volume. Samples were preserved and stored in 5% buffered formalin. The laboratory protocol was to separate samples into two size fractions by passing them through large (200 or 500 μm) then small (64 μm) mesh sieves; split, dilute, and sub-sample each size fraction as needed; count and identify all plankters (minimum n = 50) in each subsample; and estimate the length and width of exactly 100 plankters (the first 50 from the large and small fraction, respectively) to the nearest 50 μm using a dissecting microscope. Three replicate small size fractions per sample were processed starting in 2006.

Data column &amp;quot;vol&amp;quot; was calculated as follows: 
vol = length(unique(replicate)*aliquot/beaker/2^splits*50/s_per_50l*d_min*60*1e-3

Data column &amp;quot;flag&amp;quot; contains the following codes:
flag 1: length &amp;amp; width were not measured, but estimated from similar samples
flag 2: shallow &amp;amp; deep samples not labeled, but deduced from sample contents
flag 3: depth estimate less accurate than usual (± ~3 m) 
flag 4: time estimate less accurate than usual (± ~15 min)
flag 5: pump flow rate estimated by nominal flow rate</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54725.rdf" xlink:title="OCE-1043261" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1043261 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1043261</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54830.rdf" xlink:title="OCE-1043248" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1043248 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1043248</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54947.rdf" xlink:title="OCE-1043249" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1043249 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1043249</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/748455.rdf" xlink:title="NA06NOS4780148" xlink:actuate="onRequest">Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: NA06NOS4780148 Award URL: https://grantsonline.rdc.noaa.gov/flows/publicSearch/showAwardDetails.do?awdNum=NA06NOS4780148</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/748458.rdf" xlink:title="NA09NOS4780198" xlink:actuate="onRequest">Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: NA09NOS4780198 Award URL: https://grantsonline.rdc.noaa.gov/flows/publicSearch/showAwardDetails.do?awdNum=NA09NOS4780198</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/748463.rdf" xlink:title="NAS-GRP-2000006418" xlink:actuate="onRequest">Funding provided by Gulf Research Program of the National Academies of Sciences, Engineering, and Medicine (GRP) Award Number: NAS-GRP-2000006418 Award URL: http://www.nas.edu/gulf/grants/grantees/index.htm</gmx:Anchor>
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A synthesis of data collected in the Northern Gulf of Mexico from 2003-2004, 2006-2008 and 2010&lt;br /&gt;
Data include:&lt;br /&gt;
- CTD Profiles&lt;br /&gt;
- Rosette Samples&lt;br /&gt;
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- Towed SCANFISH&lt;br /&gt;
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&lt;p&gt;&lt;img alt=&quot;&quot; src=&quot;https://datadocs.bco-dmo.org/d2/NGOMEX/NGOMEX_Overall_Approach_By_Year.jpg&quot; /&gt;&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;References:&lt;/strong&gt;&lt;br /&gt;
Kimmel, D. G., W. C. Boicourt, J. J. Pierson, M. R. Roman, X. Zhang. 2010. The vertical distribution and diel variability of mesozooplankton biomass, abundance and size in response to hypoxia in the northern Gulf of Mexico USA. Journal of Plankton Research 32(8): 1185-1202. doi:10.1093/plankt/fbp136&lt;/p&gt;
&lt;p&gt;Pierson, J. J., M. R. Roman, D. G. Kimmel, W. C. Boicourt, &amp;amp; X. Zhang. 2009. Quantifying changes in the vertical distribution of mesozooplankton in response to hypoxic bottom waters. Journal of Experimental Marine Biology and Ecology 381: S74-S79. doi.org/10.1016/j.jembe.2009.07.013&lt;/p&gt;
&lt;p&gt;Kimmel, D. G., W. C. Boicourt, J. J. Pierson, M. R. Roman, &amp;amp; X. Zhang. 2009. A comparison of the mesozooplankton response to hypoxia in Chesapeake Bay and the northern Gulf of Mexico using the biomass size spectrum. Journal of Experimental Marine Biology and Ecology 381: S65-S73. doi.org/10.1016/j.jembe.2009.07.012&lt;/p&gt;
&lt;p&gt;Zhang, H., S. A. Ludsin, D. M. Mason, A. T. Adamack, S. B. Brandt, X. Zhang, D. G. Kimmel, M. R. Roman, &amp;amp; W. C. Boicourt. 2009. Hypoxia-driven changes in the behavior and spatial distribution of pelagic fish and mesozooplankton in the northern Gulf of Mexico. Journal of Experimental Marine Biology and Ecology. 381: S80-91. &lt;a href=&quot;http://dx.doi.org/10.1016/j.jembe.2009.07.014&quot;&gt;http://dx.doi.org/10.1016/j.jembe.2009.07.014&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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Sampling and analytical procedures: 
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flag 2: shallow &amp;amp; deep samples not labeled, but deduced from sample contents
flag 3: depth estimate less accurate than usual (± ~3 m) 
flag 4: time estimate less accurate than usual (± ~15 min)
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