http://lod.bco-dmo.org/id/dataset/746185 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2018-09-14 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Metabolomic fingerprints of individual algal cells using the Single-Probe Mass Spectrometry technique from experiments conducted between May and August of 2017 2018-09-21 publication 2018-09-21 revision BCO-DMO Linked Data URI 2018-09-21 creation http://lod.bco-dmo.org/id/dataset/746185 Boris Wawrik University of Oklahoma principalInvestigator Zhibo Yang University of Oklahoma principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Wawrik, B., Yang, Z. (2018) Metabolomic fingerprints of individual algal cells using the Single-Probe Mass Spectrometry technique from experiments conducted between May and August of 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-21 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/746185 [access date] Dataset Description: &lt;p&gt;These data were published in Sun et al., 2018.&lt;/p&gt; &lt;p&gt;A tabular version of this dataset is accessible&amp;nbsp;by clicking the &quot;Get Data&quot; button on this page.&amp;nbsp; The columns of the tabular dataset are described in the &quot;Parameters&quot; section on this page.&amp;nbsp; This dataset is available as&amp;nbsp;standard MS file format (.mzML) files within the following tar.gz file:&lt;br /&gt; &lt;a href=&quot;https://datadocs.bco-dmo.org/docs/Creatine_Cycling/746185/1/data_docs/Algal_Cell_Data_mzML.tar.gz&quot; target=&quot;_blank&quot;&gt;Algal_Cell_Data_mzML.tar.gz&lt;/a&gt;&amp;nbsp;(2.4 GB, contains 20 .mzML files)&lt;/p&gt; &lt;p&gt;.mzML is a standard MS file format that can be viewed using freeware such as&amp;nbsp;mMass&amp;nbsp;(http://www.mmass.org/) and ProteoWizard (http://proteowizard.sourceforge.net/index.shtml).&lt;/p&gt; Methods and Sampling: Methodology The following are excerpts from Sun et al., 2018. Please refer to this publication for more details. Non-axenic Scrippsiella trochoidea CCMP 3099 was originally obtained from the National Center for Marine Algae and Microbiota (Provasoil-Guillard NCMA, Boothbay Harbor, ME, United States). For maintenance, cultures were grown in L1 seawater media (Guillard and Ryther, 1962; Guillard et al., 1973; Guillard and Hargraves, 1993). Medium was prepared from natural seawater collected near Key West (salinity of 33), which was aged for at least 6 month in the dark and autoclaved. Maintenance and experimental cultures were grown in a light/dark incubator at 23–24°C and 30–40 μmol quanta⋅m-2 s-1 light under a 12-h light:12-h dark cycle. For the light/dark comparisons, cultures were grown under replete conditions in full L1 media containing 880 μM NaNO3 and 36 μM NaH2PO4 (N/P ratio 24:1). Experimental cultures were started as a 1:10 inoculum from exponentially growing cultures into 1 L of media in 2.5 L Pyrex Fernbach flasks without shaking and monitored daily via cell counts and chlorophyll measurements. Cell counts were conducted by addition of 1% Lugol’s iodine and direct counting of cells in 96-well microtiter plates using a dissection microscope. Dilutions were made as necessary and at least 10 wells containing 100 μL diluted culture were counted to average cell counts. Chlorophyll a was measured via fluorometry (Welschmeyer, 1994) by filtering 5 mL of culture in triplicate onto GF/F filters, over-night extraction with methanol, and quantification using a Turner Trilogy Laboratory Fluorometer. Cultures were grown into late-log phase (data not shown) and then sampled 3 h before and 3 h after the light was turned on. Cultures were sub-sampled for MS analysis, making sure to keep ‘dark’ sample exposure to light to a minimum by wrapping sampling tubes in aluminum foil. N-deplete cultures were generated by first growing cells on L1 media with an N:P ratio of 2.4:1 (88 mM nitrate: 36 mM phosphate) analogous what has been previously described (Harke et al., 2017). This lower ratio stoichiometrically limited cultures in nitrogen and at least three transfers were performed to ensure no carryover from higher nutrient full L1 medium. Cultures were monitored daily via cell counts (see above), chlorophyll a quantification (see above), and quantification of nitrate/nitrite via a Vanadium reduction method (Miranda et al., 2001). Parallel cultures were set up in which one culture was allowed to run out of nitrogenous nutrients (N-deplete), while the culture (control) was fed additional nitrate every second day to bring total nitrate/nitrite concentrations back to starting levels. Once nitrate/nitrite levels dropped below the limit of detection (∼1–2 μM) in the N-deplete culture, cultures were grown for an additional 24 h before sampling to ensure that N-depletion was complete. Both the N-deplete and replete cultures were then sampled for MS analysis of single cells. Individual cells of S. trochoidea were analyzed via the ‘Single-probe’ MS techniques. For the analysis, cells were deposited onto 0.2 μm polycarbonate membranes by gentle filtration, and the cells were rinsed with phosphate buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.47 mM KH2PO4; pH of 7.4) to remove culture medium. Filters were then placed on a home-built XYZ-translation stage system and spatial motion was controlled by a custom designed LabView software package (Lanekoff et al., 2012). The Single-probe tip (&lt;10 μm) was then precisely insert into single S. trochoidea cells (typically ∼20–30 μm cellular diameter) using a microscope as a guide. During the experiment, a syringe (250 μl; Hamilton, Co., Reno, NV, United States) was used to continuously provide the sampling solvent (acetonitrile; Sigma-Aldrich, St. Louis, MO, United States), and a liquid junction formed at the Single-probe tip to perform highly efficient extraction of cellular contents. The analytes were withdrawn by capillary action toward the nano-ESI emitter, and ionized for analysis using a Thermo LTQ Orbitrap XL mass spectrometer (Thermo Scientific, Waltham, MA, United States). Mass analyze parameters were as follows: mass resolution 60,000, +4 kV ionization voltage at positive ion mode (0.05–0.07 μA of ion current), 1 microscan, 100 ms max injection time, and automatic gain control on. Data processing: The Thermo Xcalibur Qual Browser (Thermo Scientific, Waltham, MA, United States) was used to export MS data (m/z values with relative intensities) as tab-delimited data files. As a conservative approach, only relatively abundant peaks with ion intensities &gt; 103 were exported. This approach excluded 6% of low signal peaks as background while retaining 94% of total signal intensity. The relative ion intensities were normalized to the total ion current to minimize the influence induced by fluctuations of ion signals during experiments. The Geena 2 online software tool1 was then used for peak alignment (Romano et al., 2016), and the aligned m/z values were then used for comparisons. Parameters used in Geena 2 include analysis range (from 100 to 1500 m/z), maximum number of isotopic replicas (5), maximum delta between isotopic peaks (0.05 Da), and maximum delta for aligning replicates (0.01 Da). Metaboanalyst 3.02 was used to conduct statistical data analysis, including PLS-DA (partial least squares discriminant analysis) and t-tests (Xia et al., 2015; Xia and Wishart, 2016). PLS-DA was used to visualize differences in chemical composition profiles among treatment groups, while t-tests were applied to extract molecular peaks with significant abundance changes (p &lt; 0.05). Finally, the online database METLIN3 was used to tentatively label all ions of interest (Smith et al., 2005; Guijas et al., 2018), to perform hierarchical clustering, and to generate heat maps. Lastly, Pathos4 was used to attempt identification of significantly regulated metabolic pathways by considering all KEGG maps in all organisms (Leader et al., 2011). Single-Probe description: Detailed fabrication protocols of the Single-probe have been previously described (Pan et al., 2014; Rao et al., 2015; Sun et al., 2017). Briefly, a Single-probe has three components: a dual-bore quartz tubing [outer diameter (OD) 500 μm; inner diameter (ID) 127 μm, Friedrich &amp; Dimmock, Inc., Millville, NJ, United States] pulled using a laser pipette puller (P-2000 micropipette puller, Sutter Instrument, Novato, CA, United States), a fused silica capillary (OD 105 μm; ID 40 μm, Polymicro Technologies, Phoenix, AZ, United States), and a nano-ESI emitter made from the same type of fused silica capillary. A Single-probe is fabricated by embedding a fused silica capillary and a nano-ESI emitter into both of the channels of the laser-pulled dual-bore quartz needle. Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1634630 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1634630 onGoing Boris Wawrik University of Oklahoma 301-903-4742 Burke VA USA Boris.Wawrik@science.doe.gov pointOfContact Zhibo Yang University of Oklahoma 405-325-1772 101 Stephenson Parkway Department of Chemistry and Biochemistry Norman Oklahoma 73019 USA zhibo.yang@ou.edu pointOfContact asNeeded Dataset Version: 1 Unknown light1_cell1_mz light1_cell1_intensity light1_cell2_mz light1_cell2_intensity light1_cell3_mz light1_cell3_intensity light1_cell4_mz light1_cell4_intensity light1_cell5_mz light1_cell5_intensity light1_cell6_mz light1_cell6_intensity light2_cell1_mz light2_cell1_intensity light2_cell2_mz light2_cell2_intensity light3_cell1_mz light3_cell1_intensity light3_cell2_mz light3_cell2_intensity light3_cell3_mz light3_cell3_intensity light3_cell4_mz light3_cell4_intensity light3_cell5_mz light3_cell5_intensity light3_cell6_mz light3_cell6_intensity light4_cell1_mz light4_cell1_intensity light4_cell2_mz light4_cell2_intensity light4_cell3_mz light4_cell3_intensity light4_cell4_mz light4_cell4_intensity light5_cell1_mz light5_cell1_intensity light5_cell2_mz light5_cell2_intensity light5_cell3_mz light5_cell3_intensity light5_cell4_mz light5_cell4_intensity light5_cell5_mz light5_cell5_intensity light5_cell6_mz light5_cell6_intensity dark1_cell1_mz dark1_cell1_intensity dark1_cell2_mz dark1_cell2_intensity dark2_cell1_mz dark2_cell1_intensity dark2_cell2_mz dark2_cell2_intensity dark2_cell3_mz dark2_cell3_intensity dark2_cell4_mz dark2_cell4_intensity dark2_cell5_mz dark2_cell5_intensity dark2_cell6_mz dark2_cell6_intensity dark2_cell7_mz dark2_cell7_intensity dark2_cell8_mz dark2_cell8_intensity dark3_cell1_mz dark3_cell1_intensity dark3_cell2_mz dark3_cell2_intensity dark3_cell3_mz dark3_cell3_intensity dark3_cell4_mz dark3_cell4_intensity dark3_cell5_mz dark3_cell5_intensity dark3_cell6_mz dark3_cell6_intensity dark3_cell7_mz dark3_cell7_intensity dark4_cell1_mz dark4_cell1_intensity dark4_cell2_mz dark4_cell2_intensity dark4_cell3_mz dark4_cell3_intensity dark4_cell4_mz dark4_cell4_intensity dark4_cell5_mz dark4_cell5_intensity n-limited1_cell1_mz n-limited1_cell1_intensity n-limited1_cell2_mz n-limited1_cell2_intensity n-limited1_cell3_mz n-limited1_cell3_intensity n-limited1_cell4_mz n-limited1_cell4_intensity n-limited1_cell5_mz n-limited1_cell5_intensity n-limited1_cell6_mz n-limited1_cell6_intensity n-limited1_cell7_mz n-limited1_cell7_intensity n_limited2_cell1_mz n-limited2_cell1_intensity n-limited2_cell2_mz n-limited2_cell2_intensity n_limited3_cell1_mz n-limited3_cell1_intensity n_limited4_cell1_mz n-limited4_cell1_intensity n-limited4_cell2_mz n-limited4_cell2_intensity n_limited5_cell1_mz n-limited5_cell1_intensity n-limited5_cell2_mz n-limited5_cell2_intensity n_limited6_cell1_mz n-limited6_cell1_intensity n-limited6_cell2_mz n-limited6_cell2_intensity replete1_cell1_mz replete1_cell1_intensity replete1_cell2_mz replete1_cell2_intensity replete2_cell1_mz replete2_cell1_intensity replete3_cell1_mz replete3_cell1_intensity replete3_cell2_mz replete3_cell2_intensity replete3_cell3_mz replete3_cell3_intensity replete4_cell1_mz replete4_cell1_intensity replete4_cell2_mz replete4_cell2_intensity replete4_cell3_mz replete4_cell3_intensity replete5_cell1_mz replete5_cell1_intensity replete5_cell2_mz replete5_cell2_intensity replete5_cell3_mz replete5_cell3_intensity replete5_cell4_mz replete5_cell4_intensity replete5_cell5_mz replete5_cell5_intensity replete5_cell6_mz replete5_cell6_intensity Thermo LTQ Orbitrap XL mass spectrometer theme None, User defined No BCO-DMO term featureType BCO-DMO Standard Parameters Mass Spectrometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Creatine Cycling in Marine Bacterial and Phytoplankton Assemblages https://osprey.bco-dmo.org/project/709493 Collaborative Research: Creatine Cycling in Marine Bacterial and Phytoplankton Assemblages <p><em>NSF Award Abstract:</em><br /> High rates of dissolved organic nitrogen (DON) production and utilization in aquatic systems are typically attributed to microbial activity. Though it is known that there is a tight coupling between the production and consumption of biologically available DON, the composition, dynamics, and ecological significance of this rapidly cycled DON pool are less well understood. This proposal focuses on a component of the DON pool, creatine, which is historically understood as a product of metazoan activity, but appears to be both produced by phytoplankton and consumed by marine bacteria. Creatine is present in seawater in measurable quantities, which led to the hypothesis that creatine may be a significant component of the marine DON cycle. DON cycling likely has a bearing on fundamental marine ecosystem processes with large implications for carbon and nitrogen turnover on a global scale. Broader impacts of this project will include outreach that focuses on connecting scientists with K-12 students through research experiences for teachers and lesson development in collaboration with the K20 Center for Educational and Community Renewal, a statewide education research and development center at the University of Oklahoma. The project will integrate the research with inquiry-based teaching of rural secondary science teachers through Authentic Research Experiences in oceanographic science and microbial ecology. The K20 network includes 96% of Oklahoma schools, providing a unique opportunity to impact STEM education in Oklahoma.</p> <p>The results of this project will help develop a better understanding of DON cycling, the ecological context of creatine uptake activity, and identify both creatine-producing and consuming organisms in the marine environment. The importance of creatine cycling will be assessed via 15N tracer studies along the natural coastal-to-offshore productivity gradient observed in the North Atlantic. Tracer and molecular approaches will be used to investigate the importance of phytoplankton vs. bacteria in creatine uptake and, the taxonomic identities of creatine-utilizing bacteria will be interrogated via molecular, stable isotope probing (SIP), and RT-qPCR approaches.</p> Creatine Cycling largerWorkCitation project eng; USA oceans 2017-05-01 2017-08-01 Atlantic bight 0 BCO-DMO catalogue of parameters from Metabolomic fingerprints of individual algal cells using the Single-Probe Mass Spectrometry technique from experiments conducted between May and August of 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/748198.rdf Name: light1_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748199.rdf Name: light1_cell1_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748200.rdf Name: light1_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748201.rdf Name: light1_cell2_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748202.rdf Name: light1_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748203.rdf Name: light1_cell3_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748204.rdf Name: light1_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748205.rdf Name: light1_cell4_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748206.rdf Name: light1_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748207.rdf Name: light1_cell5_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748208.rdf Name: light1_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 1 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748209.rdf Name: light1_cell6_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 1 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748210.rdf Name: light2_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 2 cell 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748211.rdf Name: light2_cell1_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 2 cell 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748212.rdf Name: light2_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 2 cell 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748213.rdf Name: light2_cell2_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 2 cell 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748214.rdf Name: light3_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748215.rdf Name: light3_cell1_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748216.rdf Name: light3_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748217.rdf Name: light3_cell2_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748218.rdf Name: light3_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate3 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748219.rdf Name: light3_cell3_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 3 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748220.rdf Name: light3_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 3 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748221.rdf Name: light3_cell4_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 3 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748222.rdf Name: light3_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 3 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748223.rdf Name: light3_cell5_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 3 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748224.rdf Name: light3_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 3 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748225.rdf Name: light3_cell6_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 3 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748226.rdf Name: light4_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748227.rdf Name: light4_cell1_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748228.rdf Name: light4_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748229.rdf Name: light4_cell2_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748230.rdf Name: light4_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate4 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748231.rdf Name: light4_cell3_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 4 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748232.rdf Name: light4_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 4 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748233.rdf Name: light4_cell4_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 4 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748234.rdf Name: light5_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748235.rdf Name: light5_cell1_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748236.rdf Name: light5_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748237.rdf Name: light5_cell2_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748238.rdf Name: light5_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate5 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748239.rdf Name: light5_cell3_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 5 cell 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748240.rdf Name: light5_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 5 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748241.rdf Name: light5_cell4_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 5 cell 4</p> http://lod.bco-dmo.org/id/dataset-parameter/748242.rdf Name: light5_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 5 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748243.rdf Name: light5_cell5_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 5 cell 5</p> http://lod.bco-dmo.org/id/dataset-parameter/748244.rdf Name: light5_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of light contition experiment replicate 5 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748245.rdf Name: light5_cell6_intensity Units: unitless Description: <p>Relative intensity of light contition experiment replicate 5 cell 6</p> http://lod.bco-dmo.org/id/dataset-parameter/748246.rdf Name: dark1_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748247.rdf Name: dark1_cell1_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748248.rdf Name: dark1_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748249.rdf Name: dark1_cell2_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748250.rdf Name: dark2_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748251.rdf Name: dark2_cell1_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748252.rdf Name: dark2_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748253.rdf Name: dark2_cell2_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748254.rdf Name: dark2_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748255.rdf Name: dark2_cell3_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748256.rdf Name: dark2_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748257.rdf Name: dark2_cell4_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748258.rdf Name: dark2_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748259.rdf Name: dark2_cell5_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748260.rdf Name: dark2_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748261.rdf Name: dark2_cell6_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748262.rdf Name: dark2_cell7_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748263.rdf Name: dark2_cell7_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748264.rdf Name: dark2_cell8_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate2 cell8</p> http://lod.bco-dmo.org/id/dataset-parameter/748265.rdf Name: dark2_cell8_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate2 cell8</p> http://lod.bco-dmo.org/id/dataset-parameter/748266.rdf Name: dark3_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748267.rdf Name: dark3_cell1_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748268.rdf Name: dark3_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748269.rdf Name: dark3_cell2_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748270.rdf Name: dark3_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748271.rdf Name: dark3_cell3_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748272.rdf Name: dark3_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748273.rdf Name: dark3_cell4_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748274.rdf Name: dark3_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748275.rdf Name: dark3_cell5_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748276.rdf Name: dark3_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748277.rdf Name: dark3_cell6_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748278.rdf Name: dark3_cell7_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate3 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748279.rdf Name: dark3_cell7_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate3 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748280.rdf Name: dark4_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748281.rdf Name: dark4_cell1_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748282.rdf Name: dark4_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748283.rdf Name: dark4_cell2_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748284.rdf Name: dark4_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate4 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748285.rdf Name: dark4_cell3_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate4 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748286.rdf Name: dark4_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate4 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748287.rdf Name: dark4_cell4_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate4 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748288.rdf Name: dark4_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of dark contition experiment replicate4 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748289.rdf Name: dark4_cell5_intensity Units: unitless Description: <p>Relative intensity of dark contition experiment replicate4 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748290.rdf Name: n-limited1_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748291.rdf Name: n-limited1_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748292.rdf Name: n-limited1_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748293.rdf Name: n-limited1_cell2_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748294.rdf Name: n-limited1_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748295.rdf Name: n-limited1_cell3_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748296.rdf Name: n-limited1_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748297.rdf Name: n-limited1_cell4_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748298.rdf Name: n-limited1_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748299.rdf Name: n-limited1_cell5_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748300.rdf Name: n-limited1_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748301.rdf Name: n-limited1_cell6_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748302.rdf Name: n-limited1_cell7_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate1 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748303.rdf Name: n-limited1_cell7_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate1 cell7</p> http://lod.bco-dmo.org/id/dataset-parameter/748304.rdf Name: n_limited2_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748305.rdf Name: n-limited2_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748306.rdf Name: n-limited2_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate2 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748307.rdf Name: n-limited2_cell2_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate2 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748308.rdf Name: n_limited3_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748309.rdf Name: n-limited3_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748310.rdf Name: n_limited4_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748311.rdf Name: n-limited4_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748312.rdf Name: n-limited4_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748313.rdf Name: n-limited4_cell2_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748314.rdf Name: n_limited5_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748315.rdf Name: n-limited5_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748316.rdf Name: n-limited5_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748317.rdf Name: n-limited5_cell2_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748318.rdf Name: n_limited6_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate6 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748319.rdf Name: n-limited6_cell1_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate6 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748320.rdf Name: n-limited6_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of n-limited contition experiment replicate6 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748321.rdf Name: n-limited6_cell2_intensity Units: unitless Description: <p>Relative intensity of n-limited contition experiment replicate6 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748322.rdf Name: replete1_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748323.rdf Name: replete1_cell1_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate1 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748324.rdf Name: replete1_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replete contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748325.rdf Name: replete1_cell2_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate1 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748326.rdf Name: replete2_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748327.rdf Name: replete2_cell1_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate2 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748328.rdf Name: replete3_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748329.rdf Name: replete3_cell1_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate3 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748330.rdf Name: replete3_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replete contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748331.rdf Name: replete3_cell2_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate3 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748332.rdf Name: replete3_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate3 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748333.rdf Name: replete3_cell3_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate3 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748334.rdf Name: replete4_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748335.rdf Name: replete4_cell1_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate4 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748336.rdf Name: replete4_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replete contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748337.rdf Name: replete4_cell2_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate4 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748338.rdf Name: replete4_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate4 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748339.rdf Name: replete4_cell3_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate4 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748340.rdf Name: replete5_cell1_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748341.rdf Name: replete5_cell1_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate5 cell1</p> http://lod.bco-dmo.org/id/dataset-parameter/748342.rdf Name: replete5_cell2_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replete contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748343.rdf Name: replete5_cell2_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate5 cell2</p> http://lod.bco-dmo.org/id/dataset-parameter/748344.rdf Name: replete5_cell3_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate5 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748345.rdf Name: replete5_cell3_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate5 cell3</p> http://lod.bco-dmo.org/id/dataset-parameter/748346.rdf Name: replete5_cell4_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate5 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748347.rdf Name: replete5_cell4_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate5 cell4</p> http://lod.bco-dmo.org/id/dataset-parameter/748348.rdf Name: replete5_cell5_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replete contition experiment replicate5 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748349.rdf Name: replete5_cell5_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate5 cell5</p> http://lod.bco-dmo.org/id/dataset-parameter/748350.rdf Name: replete5_cell6_mz Units: dimensionless Description: <p>Mass to charge ratio (m/z) of replte contition experiment replicate6 cell6</p> http://lod.bco-dmo.org/id/dataset-parameter/748351.rdf Name: replete5_cell6_intensity Units: unitless Description: <p>Relative intensity of replete contition experiment replicate6 cell6</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 21937513 https://datadocs.bco-dmo.org/file/ZZZgx0nfQnnGBP/algae_cell_data.csv algae_cell_data.csv Primary data file for dataset ID 746185 download https://osprey.bco-dmo.org/dataset/746185/data/download download onLine dataset Methodology The following are excerpts from Sun et al., 2018. Please refer to this publication for more details. Non-axenic Scrippsiella trochoidea CCMP 3099 was originally obtained from the National Center for Marine Algae and Microbiota (Provasoil-Guillard NCMA, Boothbay Harbor, ME, United States). For maintenance, cultures were grown in L1 seawater media (Guillard and Ryther, 1962; Guillard et al., 1973; Guillard and Hargraves, 1993). Medium was prepared from natural seawater collected near Key West (salinity of 33), which was aged for at least 6 month in the dark and autoclaved. Maintenance and experimental cultures were grown in a light/dark incubator at 23–24°C and 30–40 μmol quanta⋅m-2 s-1 light under a 12-h light:12-h dark cycle. For the light/dark comparisons, cultures were grown under replete conditions in full L1 media containing 880 μM NaNO3 and 36 μM NaH2PO4 (N/P ratio 24:1). Experimental cultures were started as a 1:10 inoculum from exponentially growing cultures into 1 L of media in 2.5 L Pyrex Fernbach flasks without shaking and monitored daily via cell counts and chlorophyll measurements. Cell counts were conducted by addition of 1% Lugol’s iodine and direct counting of cells in 96-well microtiter plates using a dissection microscope. Dilutions were made as necessary and at least 10 wells containing 100 μL diluted culture were counted to average cell counts. Chlorophyll a was measured via fluorometry (Welschmeyer, 1994) by filtering 5 mL of culture in triplicate onto GF/F filters, over-night extraction with methanol, and quantification using a Turner Trilogy Laboratory Fluorometer. Cultures were grown into late-log phase (data not shown) and then sampled 3 h before and 3 h after the light was turned on. Cultures were sub-sampled for MS analysis, making sure to keep ‘dark’ sample exposure to light to a minimum by wrapping sampling tubes in aluminum foil. N-deplete cultures were generated by first growing cells on L1 media with an N:P ratio of 2.4:1 (88 mM nitrate: 36 mM phosphate) analogous what has been previously described (Harke et al., 2017). This lower ratio stoichiometrically limited cultures in nitrogen and at least three transfers were performed to ensure no carryover from higher nutrient full L1 medium. Cultures were monitored daily via cell counts (see above), chlorophyll a quantification (see above), and quantification of nitrate/nitrite via a Vanadium reduction method (Miranda et al., 2001). Parallel cultures were set up in which one culture was allowed to run out of nitrogenous nutrients (N-deplete), while the culture (control) was fed additional nitrate every second day to bring total nitrate/nitrite concentrations back to starting levels. Once nitrate/nitrite levels dropped below the limit of detection (∼1–2 μM) in the N-deplete culture, cultures were grown for an additional 24 h before sampling to ensure that N-depletion was complete. Both the N-deplete and replete cultures were then sampled for MS analysis of single cells. Individual cells of S. trochoidea were analyzed via the ‘Single-probe’ MS techniques. For the analysis, cells were deposited onto 0.2 μm polycarbonate membranes by gentle filtration, and the cells were rinsed with phosphate buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.47 mM KH2PO4; pH of 7.4) to remove culture medium. Filters were then placed on a home-built XYZ-translation stage system and spatial motion was controlled by a custom designed LabView software package (Lanekoff et al., 2012). The Single-probe tip (&lt;10 μm) was then precisely insert into single S. trochoidea cells (typically ∼20–30 μm cellular diameter) using a microscope as a guide. During the experiment, a syringe (250 μl; Hamilton, Co., Reno, NV, United States) was used to continuously provide the sampling solvent (acetonitrile; Sigma-Aldrich, St. Louis, MO, United States), and a liquid junction formed at the Single-probe tip to perform highly efficient extraction of cellular contents. The analytes were withdrawn by capillary action toward the nano-ESI emitter, and ionized for analysis using a Thermo LTQ Orbitrap XL mass spectrometer (Thermo Scientific, Waltham, MA, United States). Mass analyze parameters were as follows: mass resolution 60,000, +4 kV ionization voltage at positive ion mode (0.05–0.07 μA of ion current), 1 microscan, 100 ms max injection time, and automatic gain control on. Data processing: The Thermo Xcalibur Qual Browser (Thermo Scientific, Waltham, MA, United States) was used to export MS data (m/z values with relative intensities) as tab-delimited data files. As a conservative approach, only relatively abundant peaks with ion intensities &gt; 103 were exported. This approach excluded 6% of low signal peaks as background while retaining 94% of total signal intensity. The relative ion intensities were normalized to the total ion current to minimize the influence induced by fluctuations of ion signals during experiments. The Geena 2 online software tool1 was then used for peak alignment (Romano et al., 2016), and the aligned m/z values were then used for comparisons. Parameters used in Geena 2 include analysis range (from 100 to 1500 m/z), maximum number of isotopic replicas (5), maximum delta between isotopic peaks (0.05 Da), and maximum delta for aligning replicates (0.01 Da). Metaboanalyst 3.02 was used to conduct statistical data analysis, including PLS-DA (partial least squares discriminant analysis) and t-tests (Xia et al., 2015; Xia and Wishart, 2016). PLS-DA was used to visualize differences in chemical composition profiles among treatment groups, while t-tests were applied to extract molecular peaks with significant abundance changes (p &lt; 0.05). Finally, the online database METLIN3 was used to tentatively label all ions of interest (Smith et al., 2005; Guijas et al., 2018), to perform hierarchical clustering, and to generate heat maps. Lastly, Pathos4 was used to attempt identification of significantly regulated metabolic pathways by considering all KEGG maps in all organisms (Leader et al., 2011). Single-Probe description: Detailed fabrication protocols of the Single-probe have been previously described (Pan et al., 2014; Rao et al., 2015; Sun et al., 2017). Briefly, a Single-probe has three components: a dual-bore quartz tubing [outer diameter (OD) 500 μm; inner diameter (ID) 127 μm, Friedrich &amp; Dimmock, Inc., Millville, NJ, United States] pulled using a laser pipette puller (P-2000 micropipette puller, Sutter Instrument, Novato, CA, United States), a fused silica capillary (OD 105 μm; ID 40 μm, Polymicro Technologies, Phoenix, AZ, United States), and a nano-ESI emitter made from the same type of fused silica capillary. A Single-probe is fabricated by embedding a fused silica capillary and a nano-ESI emitter into both of the channels of the laser-pulled dual-bore quartz needle. Specified by the Principal Investigator(s) BCO-DMO Data Manager Processing Notes: * added a conventional header with dataset name, PI name, version date * modified parameter names to conform with BCO-DMO naming conventions Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Thermo LTQ Orbitrap XL mass spectrometer Thermo LTQ Orbitrap XL mass spectrometer PI Supplied Instrument Name: Thermo LTQ Orbitrap XL mass spectrometer PI Supplied Instrument Description:Thermo LTQ Orbitrap XL mass spectrometer (Thermo Scientific, Waltham, MA, United States). Mass analyze parameters were as follows: mass resolution 60,000, +4 kV ionization voltage at positive ion mode (0.05–0.07 μA of ion current), 1 microscan, 100 ms max injection time, and automatic gain control on. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/