http://lod.bco-dmo.org/id/dataset/746398
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-09-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradiance
2018-09-18
publication
2018-09-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-09-30
publication
https://doi.org/10.26008/1912/bco-dmo.746398.1
Edward Laws
Louisiana State University College of the Coast and Environment
principalInvestigator
Uta Passow
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Laws, E., Passow, U. (2020) Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradiance. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.746398.1 [access date]
Thalassiosira pseudonana cyclostat experimental results Dataset Description: <p><em>Thalassiosira </em><em>pseudonanana</em> were grown in nitrate limited culture at five temperatures, 400 umol photons m–2 s–1, and 1000 ppm CO2 and irradiance levels of 50 and 300&nbsp;umol photons m-2 s-1. Growth rates, photosynthetic rates, respiration&nbsp;rates, C:N&nbsp;ratio, C:Chlorophyll-a ratio, maximum quantum yield are reported.</p> Methods and Sampling: <p>The Thalassiosira pseudonana culture was grown in a nitrate-limited or nitrate-replete continuous culture system on a 14:10 L:D cycle of illumination at temperatures of 10, 15, 20, 25, and 30°C. The irradiance during the photoperiod was either 50 or 300 mmol photons m–2 s–1. Continuous aeration of the culture was with 0.2 µm-filtered ambient air with a CO2 concentration of either 400 or 1000 ppm. Photosynthetically active radiation (400–700 nm) was measured with a Biospherical Instruments model QSL 2100 quantum sensor. Temperature was controlled to within 0.1°C by circulating water from a Haake model DC10 temperature-controlled water bath through the outer jacket of the reaction chamber. The dilution rate of the growth chamber was controlled with a peristaltic pump (Masterflex Model 77200-60) to within ± 0.002 per day. For 400 ppm CO2 treatments, the CO2 concentration in the laboratory was monitored with a nondispersive infrared absorption-based CO2 meter (AZ-0004, CO2meter.com, Ormond Beach, FL) calibrated at 0 and 400 ppm CO2 with standard gas mixture. For elevated CO2 treatments (1000 ppm), the growth chambers and media reservoirs were bubbled constantly (2–5 L/min each) with sterile-filtered ambient air amended with 100% Bone-Dry 3.0 grade CO2 (Airgas, Inc., Radnor, PA) using the system described in Fig. 1. The CO2 concentration of the amended air was monitored using a CO2 meter as for 400 ppm treatments.</p>
<p>See Figure 1 for Schematic of CO2 amendment and distribution. (See Supplemental Documents below)</p>
<p>The system was judged to be in steady state when cell counts, measured with a Beckman Coulter model Z1 particle counter, and been reproducible to within 2% for at least 4 doubling times. Chlorophyll a concentrations were determined from samples collected on glass fiber filters and extracted in methanol. The absorbances were measured at 664 and 750 nm with a Cary Model 50 spectrophotometer. Total-scale pH was measured with a Thermo Spectronic Helios spectrophotometer, as described in SOP 6B by Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. with minor modifications, and with a Hach SensION model PH31 pH meter calibrated with standards on the total pH scale, prepared as per Millero, F.J., et al. "The use of buffers to measure the pH of seawater." Marine Chemistry 44.2 (1993): 143-152. with minor modifications. The growth medium consisted of artificial seawater with a total alkalinity of 2.365 meq L–1. Nutrient concentrations corresponded to f/2 medium, with the exception of nitrate, which was added at a concentration of 40 mM. The medium was sterile filtered (0.2 mm) into a 40-liter glass carboy that had been previously autoclaved. The growth chamber was an autoclaved glass reaction flask with a working volume of 2143 mL. The cells in the growth chamber were uniformly labeled with C-14 by adding 20 microcuries of C-14 bicarbonate to the nutrient reservoir. Five-milliliter samples for C-14 activity in the organic carbon were withdrawn in triplicate from the growth chamber at two-hour intervals. The samples were acidified with 1 mL of 1 N HCl to drive off inorganic carbon. The activity of C-14 in the samples was then determined by counting on a Packard Tri-Carb model 3100 TR liquid scintillation counter. Short-term (5-minute) photosynthesis versus irradiance curves were measured at the start, middle, and end of the photoperiod. For these experiments, triplicate 5-mL aliquots from the growth chamber were added to liquid scintillation vials pre-inoculated with 0.85 microcuries of C-14 bicarbonate. The vials were incubated at irradiances of 0, 5, 10, 20, 30, 55, 80, 120, 150, 200, 250, 300, and 350 mmol photons m–2 s–1 for 5 minutes. Fixation was stopped by adding 0.5 mL of 1 N HCl to the vials. Total alkalinity was determined using the open cell titration method described as SOP 3B by Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. DIC and equilibrium CO2 concentrations were then calculated from temperature, salinity, total alkalinity, and pH using the equations in Zeebe and Wolf-Gladrow, CO2 in Seawater: Equilibrium, Kinetics, Isotopes. Photosynthetic rates as a function of irradiance were fit via least squares with the following functions: piecewise linear, simple hyperbola, hyperbolic tangent, and negative exponential. Each of these functions is described by two parameters, the light-saturated photosynthetic rate (Pmax) with units of grams carbon per gram chlorophyll a per hour and a parameter Ek with units of mmol photons m–2 s–1 that determines the characteristics of the function at irradiances sub-saturating irradiances. For example, for a simple hyperbola, Ek is the irradiance at which the photosynthetic rate equals one-half Pmax. Dark-adapted photosynthetic quantum yield (QY) was measured in triplicate for each continuous culture in steady state at mid-photoperiod. QY measurements were made with a PSI AquaPen C100 with manufacturer’s supplied plastic cuvettes containing 4 mL of culture each. Dark-adaptation of the culture samples was achieved by wrapping each of three cuvettes in aluminum foil and incubating at the treatment temperature for 30 minutes, after which QY was measured in a darkened room.&nbsp;</p>
<p>See Figure 2 for plotted results of Thalassiosia pseudonana CO2 and light experiments. (See Supplemental Documents below)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536581 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536581
completed
Edward Laws
Louisiana State University College of the Coast and Environment
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date
temp
Limiting_factor
irradiance
pCO2
treatment
growth
PI
dark_resp
Fv_Fm
PM_lights_on
PM_midday
PM_lights_off
PM_mean
Ek_lights_on
Ek_midday
Ek_lights_off
Ek_mean
min_quanta_lights_on
min_quanta_midday
min_quanta_lights_off
min_quanta_mean
C_to_N
C_to_Chl
P_to_Pm
Hach SensION model PH31 pH meter
Varian Cary Model 50 spectrophotometer
an Exeter Analytical model CE-440 elemental analyzer
Beckman Coulter model Z1 particle counter
PSI AquaPen C100
Packard Tri-Carb model 3100 TR liquid scintillation counter
CO2METER model AZ-004
Masterflex Model 77200-60 peristaltic pump
Biospherical Instruments model QSL 2100 quantum sensor
Thermo Spectronic Heios spectrophotometer
theme
None, User defined
date
water temperature at measurement depth
treatment
irradiance
Partial pressure of CO2
growth
photosynthetic rate
dark on-deck incubation oxygen respiration
Fv to Fm ratio
max photosynthesis
No BCO-DMO term
Carbon to Nitrogen ratio (C:N)
featureType
BCO-DMO Standard Parameters
Benchtop pH Meter
Cary 50 spectrophotometer
Chemostat
CHN Elemental Analyzer
Coulter Counter
Fluorometer
Liquid Scintillation Counter
pCO2 Sensor
Pump
Radiometer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2016-01-25
2018-07-22
0
BCO-DMO catalogue of parameters from Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradiance
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/746445.rdf
Name: date
Units: date
Description: <p>measurement date formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/746446.rdf
Name: temp
Units: degrees Celsius
Description: <p>target treatment temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/746447.rdf
Name: Limiting_factor
Units: unitless
Description: <p>Limiting factor for photosynthesis: either nitrate or temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/746448.rdf
Name: irradiance
Units: micro-mol photons per square meter per second (mmol photons/m^2/s)
Description: <p>irradiance during photoperiod</p>
http://lod.bco-dmo.org/id/dataset-parameter/746449.rdf
Name: pCO2
Units: parts per million by volume (ppmv)
Description: <p>pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/746450.rdf
Name: treatment
Units: unitless
Description: <p>experimental treatment: high and low levels of irradiance and temperature (in that order): CO2 treatments: L = 400 ppm CO2; H = 1000 ppm CO2; irradiance tmt: L = 50 umol photons m-2 s-1; H = 300 umol photons m-2 s-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/746451.rdf
Name: growth
Units: per day
Description: <p>growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/746452.rdf
Name: PI
Units: grams Carbon per gram chlorophpyll per hour (gC/g chl/hour)
Description: <p>mean Photosynthetic Irradiance</p>
http://lod.bco-dmo.org/id/dataset-parameter/746453.rdf
Name: dark_resp
Units: per day
Description: <p>dark respiration rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/746454.rdf
Name: Fv_Fm
Units: dimensionless
Description: <p>The normalized ratio of variable fluorescence to maximum fluorescence; Fv/Fm</p>
http://lod.bco-dmo.org/id/dataset-parameter/746455.rdf
Name: PM_lights_on
Units: grams Carbon per gram chlorophpyll per hour (gC/g chl/hour)
Description: <p>Maximum photosynthesis (Pmax) when growth chamber lights first come on.</p>
http://lod.bco-dmo.org/id/dataset-parameter/746456.rdf
Name: PM_midday
Units: grams Carbon per gram chlorophpyll per hour (gC/g chl/hour)
Description: <p>Maximum photosynthesis (Pmax) at midday</p>
http://lod.bco-dmo.org/id/dataset-parameter/746457.rdf
Name: PM_lights_off
Units: grams Carbon per gram chlorophpyll per hour (gC/g chl/hour)
Description: <p>Maximum photosynthesis (Pmax) when the lights go off.</p>
http://lod.bco-dmo.org/id/dataset-parameter/746458.rdf
Name: PM_mean
Units: grams Carbon per gram chlorophpyll per hour (gC/g chl/hour)
Description: <p>mean maximum photosynthesis (Pmax); calculated for the 14-hour photoperiod.</p>
http://lod.bco-dmo.org/id/dataset-parameter/746459.rdf
Name: Ek_lights_on
Units: micro-mol photons per square meter per second (umol photons/m^2/s)
Description: <p>the light saturation index or saturation irradiance (Ek) when the lights first come on.</p>
http://lod.bco-dmo.org/id/dataset-parameter/746460.rdf
Name: Ek_midday
Units: micro-mol photons per square meter per second (umol photons/m^2/s)
Description: <p>the light saturation index or saturation irradiance (Ek) at midday</p>
http://lod.bco-dmo.org/id/dataset-parameter/746461.rdf
Name: Ek_lights_off
Units: micro-mol photons per square meter per second (umol photons/m^2/s)
Description: <p>the light saturation index or saturation irradiance (Ek) at lights go off</p>
http://lod.bco-dmo.org/id/dataset-parameter/746462.rdf
Name: Ek_mean
Units: micro-mol photons per square meter per second (umol photons/m^2/s)
Description: <p>the mean light saturation index or saturation irradiance (Ek)</p>
http://lod.bco-dmo.org/id/dataset-parameter/746463.rdf
Name: min_quanta_lights_on
Units: mol quanta per mol Carbon (mol quanta/mol C )
Description: <p>minimum mol quanta per mol C at lights on</p>
http://lod.bco-dmo.org/id/dataset-parameter/746464.rdf
Name: min_quanta_midday
Units: mol quanta per mol Carbon (mol quanta/mol C )
Description: <p>minimum mol quanta per mol C at midday</p>
http://lod.bco-dmo.org/id/dataset-parameter/746465.rdf
Name: min_quanta_lights_off
Units: mol quanta per mol Carbon (mol quanta/mol C )
Description: <p>minimum mol quanta per mol C at lights off</p>
http://lod.bco-dmo.org/id/dataset-parameter/746466.rdf
Name: min_quanta_mean
Units: mol quanta per mol Carbon (mol quanta/mol C )
Description: <p>mean of minimum quanta per mol C</p>
http://lod.bco-dmo.org/id/dataset-parameter/746467.rdf
Name: C_to_N
Units: grams per grams
Description: <p>carbon to nitrogen content ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/746468.rdf
Name: C_to_Chl
Units: grams per grams
Description: <p>ratio of carbon to chlorophyl a</p>
http://lod.bco-dmo.org/id/dataset-parameter/746469.rdf
Name: P_to_Pm
Units: dimensionless
Description: <p>ratio of photosynthesis to maximum photosynthesis</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5296
https://darchive.mblwhoilibrary.org/bitstream/1912/26244/1/dataset-746398_thalassiosira-pseudonana-cyclostat__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.746398.1
download
onLine
dataset
<p>The Thalassiosira pseudonana culture was grown in a nitrate-limited or nitrate-replete continuous culture system on a 14:10 L:D cycle of illumination at temperatures of 10, 15, 20, 25, and 30°C. The irradiance during the photoperiod was either 50 or 300 mmol photons m–2 s–1. Continuous aeration of the culture was with 0.2 µm-filtered ambient air with a CO2 concentration of either 400 or 1000 ppm. Photosynthetically active radiation (400–700 nm) was measured with a Biospherical Instruments model QSL 2100 quantum sensor. Temperature was controlled to within 0.1°C by circulating water from a Haake model DC10 temperature-controlled water bath through the outer jacket of the reaction chamber. The dilution rate of the growth chamber was controlled with a peristaltic pump (Masterflex Model 77200-60) to within ± 0.002 per day. For 400 ppm CO2 treatments, the CO2 concentration in the laboratory was monitored with a nondispersive infrared absorption-based CO2 meter (AZ-0004, CO2meter.com, Ormond Beach, FL) calibrated at 0 and 400 ppm CO2 with standard gas mixture. For elevated CO2 treatments (1000 ppm), the growth chambers and media reservoirs were bubbled constantly (2–5 L/min each) with sterile-filtered ambient air amended with 100% Bone-Dry 3.0 grade CO2 (Airgas, Inc., Radnor, PA) using the system described in Fig. 1. The CO2 concentration of the amended air was monitored using a CO2 meter as for 400 ppm treatments.</p>
<p>See Figure 1 for Schematic of CO2 amendment and distribution. (See Supplemental Documents below)</p>
<p>The system was judged to be in steady state when cell counts, measured with a Beckman Coulter model Z1 particle counter, and been reproducible to within 2% for at least 4 doubling times. Chlorophyll a concentrations were determined from samples collected on glass fiber filters and extracted in methanol. The absorbances were measured at 664 and 750 nm with a Cary Model 50 spectrophotometer. Total-scale pH was measured with a Thermo Spectronic Helios spectrophotometer, as described in SOP 6B by Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. with minor modifications, and with a Hach SensION model PH31 pH meter calibrated with standards on the total pH scale, prepared as per Millero, F.J., et al. "The use of buffers to measure the pH of seawater." Marine Chemistry 44.2 (1993): 143-152. with minor modifications. The growth medium consisted of artificial seawater with a total alkalinity of 2.365 meq L–1. Nutrient concentrations corresponded to f/2 medium, with the exception of nitrate, which was added at a concentration of 40 mM. The medium was sterile filtered (0.2 mm) into a 40-liter glass carboy that had been previously autoclaved. The growth chamber was an autoclaved glass reaction flask with a working volume of 2143 mL. The cells in the growth chamber were uniformly labeled with C-14 by adding 20 microcuries of C-14 bicarbonate to the nutrient reservoir. Five-milliliter samples for C-14 activity in the organic carbon were withdrawn in triplicate from the growth chamber at two-hour intervals. The samples were acidified with 1 mL of 1 N HCl to drive off inorganic carbon. The activity of C-14 in the samples was then determined by counting on a Packard Tri-Carb model 3100 TR liquid scintillation counter. Short-term (5-minute) photosynthesis versus irradiance curves were measured at the start, middle, and end of the photoperiod. For these experiments, triplicate 5-mL aliquots from the growth chamber were added to liquid scintillation vials pre-inoculated with 0.85 microcuries of C-14 bicarbonate. The vials were incubated at irradiances of 0, 5, 10, 20, 30, 55, 80, 120, 150, 200, 250, 300, and 350 mmol photons m–2 s–1 for 5 minutes. Fixation was stopped by adding 0.5 mL of 1 N HCl to the vials. Total alkalinity was determined using the open cell titration method described as SOP 3B by Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. DIC and equilibrium CO2 concentrations were then calculated from temperature, salinity, total alkalinity, and pH using the equations in Zeebe and Wolf-Gladrow, CO2 in Seawater: Equilibrium, Kinetics, Isotopes. Photosynthetic rates as a function of irradiance were fit via least squares with the following functions: piecewise linear, simple hyperbola, hyperbolic tangent, and negative exponential. Each of these functions is described by two parameters, the light-saturated photosynthetic rate (Pmax) with units of grams carbon per gram chlorophyll a per hour and a parameter Ek with units of mmol photons m–2 s–1 that determines the characteristics of the function at irradiances sub-saturating irradiances. For example, for a simple hyperbola, Ek is the irradiance at which the photosynthetic rate equals one-half Pmax. Dark-adapted photosynthetic quantum yield (QY) was measured in triplicate for each continuous culture in steady state at mid-photoperiod. QY measurements were made with a PSI AquaPen C100 with manufacturer’s supplied plastic cuvettes containing 4 mL of culture each. Dark-adaptation of the culture samples was achieved by wrapping each of three cuvettes in aluminum foil and incubating at the treatment temperature for 30 minutes, after which QY was measured in a darkened room.&nbsp;</p>
<p>See Figure 2 for plotted results of Thalassiosia pseudonana CO2 and light experiments. (See Supplemental Documents below)</p>
Specified by the Principal Investigator(s)
<p>The rate of change of the concentration of particulate organic carbon in the growth chamber is described by the differential equation</p>
<p>d(POC)/dt = P – u x&nbsp;POC</p>
<p>Where POC is the concentration of particulate organic carbon, u is the dilution rate of the growth chamber, and P is the photosynthetic rate. The solution of this equation for P over the time interval t<sub>1</sub> to t<sub>2</sub> is</p>
<p>P = (POC_2 e^(u(t_2-t_1)) – POC_1 / (e^(–u(t_2 - t_1))^-1)</p>
<p>Where POC<sub>1</sub> and POC<sub>2</sub> are the concentrations of POC at times t<sub>1</sub> and t<sub>2</sub>, respectively. Photosynthetic rates were calculated on the basis of POC concentrations measured at two-hour intervals throughout the 14-h photoperiod. The photosynthetic rates so-calculated were divided by the corresponding chlorophyll a concentrations to determine the productivity indices (photosynthetic rates normalized to chlorophyll a) in units of g C g<sup>–1</sup> chl a h<sup>–1</sup> and then averaged over the photoperiod.</p>
<p>Short-term (5-minute) photosynthesis versus irradiance plots were described by the Hill equation with a Hill coefficient of 2:</p>
<p>P = (P_m * I^2) / ((E_K)^2 + I^2)</p>
<p>Where&nbsp;I&nbsp;is the irradiance,&nbsp;P&nbsp;is the photosynthetic rate,&nbsp;P_m&nbsp;is the light-saturated photosynthetic rate, and&nbsp;E_K&nbsp;is a constant. When&nbsp;I&nbsp;=&nbsp;E_K,&nbsp;P&nbsp;is equal to&nbsp;P_m/2. Based on this equation, the minimum number of photons required to fix one carbon atom is 2E_K/P_m.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- removed blank rows<br />
- added parameter name 'treatment' to a&nbsp;column with no header<br />
- copied temp and limiting factor values to cells below within same treatment series (4 rows)<br />
- converted date format from yyyy.m.d to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hach SensION model PH31 pH meter
Hach SensION model PH31 pH meter
PI Supplied Instrument Name: Hach SensION model PH31 pH meter Instrument Name: Benchtop pH Meter Instrument Short Name:Benchtop pH Meter Instrument Description: An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
Varian Cary Model 50 spectrophotometer
Varian Cary Model 50 spectrophotometer
PI Supplied Instrument Name: Varian Cary Model 50 spectrophotometer PI Supplied Instrument Description:Used to measure absorbances were measured at 664 and 750 nm Instrument Name: Cary 50 spectrophotometer Instrument Short Name:Cary 50 Instrument Description: A Cary 50 spectrophotometer measures absorbance (200-800 nm). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0523/
PI Supplied Instrument Name: Instrument Name: Chemostat Instrument Short Name: Instrument Description: Devices in which controlled conditions are maintained for a chemical process to be carried out by organisms or biochemically active substances derived from such organisms.
an Exeter Analytical model CE-440 elemental analyzer
an Exeter Analytical model CE-440 elemental analyzer
PI Supplied Instrument Name: an Exeter Analytical model CE-440 elemental analyzer PI Supplied Instrument Description:Used to measure concentrations of particulate organic carbon (POC) and particulate nitrogen (PN) Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Beckman Coulter model Z1 particle counter
Beckman Coulter model Z1 particle counter
PI Supplied Instrument Name: Beckman Coulter model Z1 particle counter PI Supplied Instrument Description:Use to make cell counts Instrument Name: Coulter Counter Instrument Short Name: Instrument Description: An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
from https://en.wikipedia.org/wiki/Coulter_counter
PSI AquaPen C100
PSI AquaPen C100
PI Supplied Instrument Name: PSI AquaPen C100 PI Supplied Instrument Description:Used to measure the maximum quantum yield, QY (Fv/Fm) with the manufacturer’s supplied plastic cuvettes containing 4 mL of culture each. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Packard Tri-Carb model 3100 TR liquid scintillation counter
Packard Tri-Carb model 3100 TR liquid scintillation counter
PI Supplied Instrument Name: Packard Tri-Carb model 3100 TR liquid scintillation counter PI Supplied Instrument Description:Used to measure the activity of C-14 in the samples Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
CO2METER model AZ-004
CO2METER model AZ-004
PI Supplied Instrument Name: CO2METER model AZ-004 PI Supplied Instrument Description:A nondispersive infrared absorption-based CO2 meter. Used to monitor CO2 concentration in the laboratory. Calibrated at 0 and 400 ppm CO2 with a standard gas mixture. Instrument Name: pCO2 Sensor Instrument Short Name:pCO2 Sensor Instrument Description: A sensor that measures the partial pressure of CO2 in water (pCO2)
Masterflex Model 77200-60 peristaltic pump
Masterflex Model 77200-60 peristaltic pump
PI Supplied Instrument Name: Masterflex Model 77200-60 peristaltic pump PI Supplied Instrument Description:Used to control the dilution rate of the growth chamber Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Biospherical Instruments model QSL 2100 quantum sensor
Biospherical Instruments model QSL 2100 quantum sensor
PI Supplied Instrument Name: Biospherical Instruments model QSL 2100 quantum sensor PI Supplied Instrument Description:Used to measure photosynthetically active radiation (400–700 nm) Instrument Name: Radiometer Instrument Short Name:Radiometer Instrument Description: Radiometer is a generic term for a range of instruments used to measure electromagnetic radiation (radiance and irradiance) in the atmosphere or the water column. For example, this instrument category includes free-fall spectral radiometer (SPMR/SMSR System, Satlantic, Inc), profiling or deck cosine PAR units (PUV-500 and 510, Biospherical Instruments, Inc). This is a generic term used when specific type, make and model were not specified. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/122/
Thermo Spectronic Heios spectrophotometer
Thermo Spectronic Heios spectrophotometer
PI Supplied Instrument Name: Thermo Spectronic Heios spectrophotometer PI Supplied Instrument Description:Used to measure pH Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/