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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/747699.rdf" xlink:actuate="onRequest">Matrix of taxon by sample for hard substrates collected by HOV Alvin during R/V Atlantis cruise AT37-13 and AT42-03 in the Pacific margin of Costa Rica in 2017 and 2018.</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Levin, L. A., Rouse, G., Pereira, O. S. (2021) Matrix of taxon by sample for hard substrates collected by HOV Alvin during R/V Atlantis cruise AT37-13 and AT42-03 in the Pacific margin of Costa Rica in 2017 and 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2021-03-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.747699.2 [access date]</gco:CharacterString>
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        <gco:CharacterString>Hard substrates matrix Dataset Description: &amp;lt;p&amp;gt;Matrix of taxon (columns) by sample (rows) for substrates collected during AT 37-13.&amp;amp;nbsp;Samples were collected with HOV Alvin.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt; In situ carbonate rocks were taken at sites with different seepage activity in 2017 (AT37-13) and 2018 (AT42-03) using HOV Alvin. These were used as control samples for two experiments: (1) Carbonate rocks, woods, and bones deployed for 7.4 years were collected across seepage gradients at Mound 12 using HOV Alvin (Colonization 7.4 years), and (2) Carbonate rocks were moved by the HOV to different seepage conditions with exposure for 17 months (Transplant 17 months).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;All substrates were photographed intact upon recovery, and wrapped in aluminum foil to determine the surface area. Before preserving, the sample was kept cold and animals were picked to sample tissue for isotope analysis (see files “AT37-13 and AT42-03 Hard substrate isotopes” for isotopic data).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The remaining sample was sieved through 0.3 mm mesh, separating the sample in two fractions (a fine fraction with the meiofauna, and a coarser one with the macrofauna), both preserved in 96% Ethanol. In the laboratory, the samples were washed in distilled water and sorted, and the animals were identified to the lowest level possible, counted and preserved in 96% Ethanol.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/710021.rdf" xlink:title="OCE-1634172" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1634172 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1634172</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;NSF abstract:&lt;/em&gt;&lt;br /&gt;
If life were to disappear from the deep sea, would we notice? We only have a cursory understanding of this vast region and the connectivity among its communities and the rest of the oceans, and yet the ecosystems of the deep sea have been implicated in the larger function of the global marine ecosystems. We now rely on the deep ocean for food, energy, novel drugs and materials, and for its role in the global cycling of carbon, as well as for supporting services such as habitat creation, nutrient replenishment for shallow waters, and the maintenance of biodiversity. Cold seeps, active areas of the seafloor where methane and other chemicals are released, are key features along the continental margins worldwide. To characterize how methane seep communities interact with the surrounding ecosystems and vice versa, we will study methane seeps off the Pacific coast of Costa Rica in 2017 and 2018. It is the sphere of influence around the seep, both along the seafloor and up into the water column, that we seek to better understand. We will map the structure and the chemistry surrounding these habitats using a novel 3-dimensional framework, combining typical transects with vertical characterizations of the water column just above the seafloor. This will include measurements of methane flux into the water column and changes in the overlying carbonate chemistry and oxygen levels that are critical to our understanding of the effect of warming, oxygen loss and ocean acidification in this region. Within this framework, we will collect seep organisms in sediments and on rocks (including all sizes from microbes to large animals), and transplant some of these from within the area of seep influence to the background deep sea, and vice-versa. Together, these studies will help us to measure the size of the seep sphere of influence, and also demonstrate the role of these seeps within the deep sea and the greater, global, marine ecosystem. We will share this information with a group of teachers during a series of workshops in the San Diego area, at an exhibit at the Birch Aquarium, and through the work of an artist who has worked extensively with marine organisms in extreme environments.&lt;/p&gt;
&lt;p&gt;Chemosynthetic ecosystems are inextricably linked to the broader world-ocean biome and global biogeochemical cycles in ways that we are just beginning to understand. This research will identify the form, extent, and nature of the physical, chemical, and biological linkages between methane seeps and the surrounding deep-sea ecosystem. The proposed research builds critical understanding of the structural and functional processes that underpin the ecosystem services provided by chemosynthetic ecosystems. We target a critical continental margin, Costa Rica, where methane fates and dynamics loom large and play out in an setting that reflects many oceanographic stressors. We will use quantitative sampling and manipulative studies within a 3-dimensional oceanographic framework. We will ask what are the shapes of the diversity and density functions for organisms of different size classes and trophic position over the transition from the seep habitat through the ecotone to the background deep sea? Further, we will ask how do depth, dissolved oxygen concentrations, pH and carbonate ion availability, relative rates of fluid flux, and substrate (biogenic, authigenic carbonate, sediments) alter these linkages and interactions with the surrounding deep sea? Evidence for distinct transitional communities and biotic patterns in density and alpha and beta diversity will be quantified and placed in a global biogeographic context. All of these investigations will occur across biological size spectra: for microorganisms (archaea, bacteria, microeukaryotes), the macrofauna, and the megafauna that form biogenic habitats. Our research results will be interpreted in the context of potential effects of global ocean change in the equatorial Pacific to determine how the linkages with the surrounding deep sea will be altered as anthropogenic impacts proceed in the future. &lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Related publications:&lt;/strong&gt;&lt;br /&gt;
Levin, L.A., V.J. Orphan, G.W. Rouse, W. Ussler, A. E. Rathburn, G. S. Cook, S. Goffredi, E. Perez, A. Waren, B. Grupe, G. Chadwick, B. Strickrott. (2012). A hydrothermal seep on the Costa Rica margin: Middle ground in a continuum of reducing ecosystems. &lt;em&gt;Proc. Royal Soc. B.&lt;/em&gt; 279: 2580-88 doi: &lt;a href=&quot;http://dx.doi.org/10.1098/rspb.2012.0205&quot; target=&quot;_blank&quot;&gt;10.1098/rspb.2012.0205&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Sahling, H., Masson, D. G., Ranero, C. R., Hühnerbach, V., Weinrebe, W., Klaucke, I., &amp;amp; Suess, E. (2008). Fluid seepage at the continental margin offshore Costa Rica and southern Nicaragua. &lt;em&gt;Geochemistry, Geophysics, Geosystems&lt;/em&gt; 9: doi: &lt;a href=&quot;http://dx.doi.org/10.1029/2008GC001978&quot; target=&quot;_blank&quot;&gt;10.1029/2008GC001978&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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http://lod.bco-dmo.org/id/dataset-parameter/845635.rdf
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	Units: decimal degrees
	Description: &lt;p&gt;Latitude of sampling location&lt;/p&gt; 
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	Description: &lt;p&gt;Longitude of sampling location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845639.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845641.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845644.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845645.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845646.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845647.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845648.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845649.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845650.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845651.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845652.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845653.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845654.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845655.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845656.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845657.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/845660.rdf
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	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845666.rdf
	Name: Nereididae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845667.rdf
	Name: Capitellidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845668.rdf
	Name: Orbiniidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845669.rdf
	Name: Pilargiidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845670.rdf
	Name: Terebellidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845671.rdf
	Name: Onuphidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845672.rdf
	Name: Eunicidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845673.rdf
	Name: Chaetopteridae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845674.rdf
	Name: Sigalionidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845675.rdf
	Name: Opheliidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845676.rdf
	Name: Arenicolidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845677.rdf
	Name: Goniadidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845678.rdf
	Name: Nephtyidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845679.rdf
	Name: Actiniaria
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845680.rdf
	Name: Hydroidolina
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845681.rdf
	Name: Alcyonacea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845682.rdf
	Name: Trombidiformes
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845683.rdf
	Name: Amphipoda
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845684.rdf
	Name: Anomura
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845685.rdf
	Name: Brachyura
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845686.rdf
	Name: Cumacea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845687.rdf
	Name: Isopoda
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845688.rdf
	Name: Tanaidacea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845689.rdf
	Name: Ostracoda
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845690.rdf
	Name: Caridea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845691.rdf
	Name: Sessilia
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845692.rdf
	Name: Mysidacea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845693.rdf
	Name: Asteroidea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845694.rdf
	Name: Ophiuroidea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845695.rdf
	Name: Holothuroidea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845696.rdf
	Name: Aplacophora
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845697.rdf
	Name: Nuculanidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845698.rdf
	Name: Juvenile
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845699.rdf
	Name: Xylophagaidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845700.rdf
	Name: Cuspidariidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845701.rdf
	Name: Mytilidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845702.rdf
	Name: Teredinidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845703.rdf
	Name: Vesicomyidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845704.rdf
	Name: Solemyidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845705.rdf
	Name: Pyramidellidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845706.rdf
	Name: Eucyclidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845707.rdf
	Name: Seguenziidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845708.rdf
	Name: Skeneidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845709.rdf
	Name: Cataegidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845710.rdf
	Name: Hyalogyniridae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845711.rdf
	Name: Provannidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845712.rdf
	Name: Lepetodrilidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845713.rdf
	Name: Cocculinidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845714.rdf
	Name: Neolepetopsidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845715.rdf
	Name: Pyropeltidae
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845716.rdf
	Name: Polyplacophora
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845717.rdf
	Name: Nemertea
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845718.rdf
	Name: Platyhelminthes
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/845719.rdf
	Name: Pycnogonida
	Units: unitless
	Description: &lt;p&gt;Number of individuals identified from taxon&lt;/p&gt; 
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